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Albrectsen, Benedicte RiberORCID iD iconorcid.org/0000-0002-9337-4540
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Publikasjoner (10 av 41) Visa alla publikasjoner
Westman, S., Kloth, K. J., Hanson, J., Ohlsson, A. B. & Albrectsen, B. R. (2019). Defence priming in Arabidopsis: a Meta-Analysis. Scientific Reports, 9, Article ID 13309.
Åpne denne publikasjonen i ny fane eller vindu >>Defence priming in Arabidopsis: a Meta-Analysis
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2019 (engelsk)Inngår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, artikkel-id 13309Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Defence priming by organismal and non-organismal stimulants can reduce effects of biotic stress in plants. Thus, it could help efforts to enhance the sustainability of agricultural production by reducing use of agrochemicals in protection of crops from pests and diseases. We have explored effects of applying this approach to both Arabidopsis plants and seeds of various crops in meta-analyses. The results show that its effects on Arabidopsis plants depend on both the priming agent and antagonist. Fungi and vitamins can have strong priming effects, and priming is usually more effective against bacterial pathogens than against herbivores. Moreover, application of bio-stimulants (particularly vitamins and plant defence elicitors) to seeds can have promising defence priming effects. However, the published evidence is scattered, does not include Arabidopsis, and additional studies are required before we can draw general conclusions and understand the molecular mechanisms involved in priming of seeds' defences. In conclusion, defence priming of plants has clear potential and application of bio-stimulants to seeds may protect plants from an early age, promises to be both labour- and resource-efficient, poses very little environmental risk, and is thus both economically and ecologically promising.

sted, utgiver, år, opplag, sider
Nature Publishing Group, 2019
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-164400 (URN)10.1038/s41598-019-49811-9 (DOI)000485861500034 ()31527672 (PubMedID)
Tilgjengelig fra: 2019-11-11 Laget: 2019-11-11 Sist oppdatert: 2019-11-11bibliografisk kontrollert
Papazian, S., Girdwood, T., Wessels, B. A., Poelman, E. H., Dicke, M., Moritz, T. & Albrectsen, B. R. (2019). Leaf metabolic signatures induced by real and simulated herbivory in black mustard (Brassica nigra). Metabolomics, 15(10), Article ID 130.
Åpne denne publikasjonen i ny fane eller vindu >>Leaf metabolic signatures induced by real and simulated herbivory in black mustard (Brassica nigra)
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2019 (engelsk)Inngår i: Metabolomics, ISSN 1573-3882, E-ISSN 1573-3890, Vol. 15, nr 10, artikkel-id 130Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Introduction The oxylipin methyl jasmonate (MeJA) is a plant hormone active in response signalling and defence against herbivores. Although MeJA is applied experimentally to mimic herbivory and induce plant defences, its downstream effects on the plant metabolome are largely uncharacterized, especially in the context of primary growth and tissue-specificity of the response. Objectives We investigated the effects of MeJA-simulated and real caterpillar herbivory on the foliar metabolome of the wild plant Brassica nigra and monitored the herbivore-induced responses in relation to leaf ontogeny. Methods As single or multiple herbivory treatments, MeJA- and mock-sprayed plants were consecutively exposed to caterpillars or left untreated. Gas chromatography (GC) and liquid chromatography (LC) time-of-flight mass-spectrometry (TOF-MS) were combined to analyse foliar compounds, including central primary and specialized defensive plant metabolites. Results Plant responses were stronger in young leaves, which simultaneously induced higher chlorophyll levels. Both MeJA and caterpillar herbivory induced similar, but not identical, accumulation of tricarboxylic acids (TCAs), glucosinolates (GSLs) and phenylpropanoids (PPs), but only caterpillar feeding led to depletion of amino acids. MeJA followed by caterpillars caused higher induction of defence compounds, including a three-fold increase in the major defence compound allyl-GSL (sinigrin). When feeding on MeJA-treated plants, caterpillars gained less weight indicative of the reduced host-plant quality and enhanced resistance. Conclusions The metabolomics approach showed that plant responses induced by herbivory extend beyond the regulation of defence metabolism and are tightly modulated throughout leaf development. This leads to a new understanding of the plant metabolic potential that can be exploited for future plant protection strategies.

sted, utgiver, år, opplag, sider
Springer, 2019
Emneord
Metabolomics, Methyl jasmonate, Brassica nigra, Growth-defence allocation, Priming, Herbivore-induced responses, Leaf ontogeny, Glucosinolates
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-164033 (URN)10.1007/s11306-019-1592-4 (DOI)000487922100001 ()31563978 (PubMedID)
Tilgjengelig fra: 2019-10-16 Laget: 2019-10-16 Sist oppdatert: 2019-10-16bibliografisk kontrollert
Kloth, K. J., Abreu, I. N., Delhomme, N., Petrik, I., Villard, C., Ström, C., . . . Albrectsen, B. R. (2019). PECTIN ACETYLESTERASE9 Affects the Transcriptome and Metabolome and Delays Aphid Feeding1[OPEN]. Plant Physiology, 181(4), 1704-1720
Åpne denne publikasjonen i ny fane eller vindu >>PECTIN ACETYLESTERASE9 Affects the Transcriptome and Metabolome and Delays Aphid Feeding1[OPEN]
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2019 (engelsk)Inngår i: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 181, nr 4, s. 1704-1720Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The plant cell wall plays an important role in damage-associated molecular pattern-induced resistance to pathogens and herbivorous insects. Our current understanding of cell wall-mediated resistance is largely based on the degree of pectin methylesterification. However, little is known about the role of pectin acetylesterification in plant immunity. This study describes how one pectin-modifying enzyme, PECTIN ACETYLESTERASE 9 (PAE9), affects the Arabidopsis (Arabidopsis thaliana) transcriptome, secondary metabolome, and aphid performance. Electro-penetration graphs showed that Myzus persicae aphids established phloem feeding earlier on pae9 mutants. Whole-genome transcriptome analysis revealed a set of 56 differentially expressed genes (DEGs) between uninfested pae9-2 mutants and wild-type plants. The majority of the DEGs were enriched for biotic stress responses and down-regulated in the pae9-2 mutant, including PAD3 and IGMT2, involved in camalexin and indole glucosinolate biosynthesis, respectively. Relative quantification of more than 100 secondary metabolites revealed decreased levels of several compounds, including camalexin and oxylipins, in two independent pae9 mutants. In addition, absolute quantification of phytohormones showed that jasmonic acid (JA), jasmonoyl-Ile, salicylic acid, abscisic acid, and indole-3-acetic acid were compromised due to PAE9 loss of function. After aphid infestation, however, pae9 mutants increased their levels of camalexin, glucosinolates, and JA, and no long-term effects were observed on aphid fitness. Overall, these data show that PAE9 is required for constitutive up-regulation of defense-related compounds, but that it is not required for aphid-induced defenses. The signatures of phenolic antioxidants, phytoprostanes, and oxidative stress-related transcripts indicate that the processes underlying PAE9 activity involve oxidation-reduction reactions. PECTIN ACETYLESTERASE9 is involved in the accumulation of jasmonic acid, camalexin and antioxidants, and delays establishment of aphid phloem feeding, but is not required for aphid-induced defenses.

sted, utgiver, år, opplag, sider
Rockville: American Society of Plant Biologists, 2019
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-167072 (URN)10.1104/pp.19.00635 (DOI)000501767700025 ()31551361 (PubMedID)
Tilgjengelig fra: 2020-01-13 Laget: 2020-01-13 Sist oppdatert: 2020-01-13bibliografisk kontrollert
Albrectsen, B. R., Siddique, A. B., Decker, V. H., Unterseher, M. & Robinson, K. M. (2018). Both plant genotype and herbivory shape aspen endophyte communities. Oecologia, 187(2), 535-545
Åpne denne publikasjonen i ny fane eller vindu >>Both plant genotype and herbivory shape aspen endophyte communities
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2018 (engelsk)Inngår i: Oecologia, ISSN 0029-8549, E-ISSN 1432-1939, Vol. 187, nr 2, s. 535-545Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Salicinoid phenolic glycosides are common defence substances in salicaceous trees and specialist leaf beetles use these compounds for their own defence against predators. Salicinoids vary qualitatively and qualitatively in aspen (Populus tremula) and this variation has a genetic basis. The foliar endophyte mycobiome is plentiful and we hypothesised that it is related to plant genotype, potentially mediated by salicinoid composition, and that interactions with the leaf beetle Chrysomela tremula may alter this relationship. We studied these three-way interactions in controlled greenhouse experiments. Endophytic fungi were isolated from sterilised leaf tissues with and without beetle damage, and from beetles. We confirmed that endophyte composition was influenced by host genotype. Beetle activity added generalist morphs to the mycobiome that overrode the initial host association. Yeast-like genera (Cryptococcus and Rhodotorula) were isolated only from beetle-damaged tissues and from beetles, whereas fast-growing filamentous fungi dominated beetle-free control plants. Competition experiments between filamentous fungi of plant origin and beetle-related yeasts suggested interaction of both stimulating and inhibiting modes of action amongst the fungi. As a result, we detected examples of amensalism, commensalism, parasitism and competition between the morphs tested, but we found no evidence of mutualism, and consequently no co-evolutionary relationship could be demonstrated, between yeasts carried by beetles, host genotype and associated filamentous morphs. Endophyte studies are method-dependent and high-throughput sequencing technology best define the fungal mycobiome, culturing however continues to be a cheap way to provide fundamental ecological insights and it is also required for experimental studies.

sted, utgiver, år, opplag, sider
Springer, 2018
Emneord
Herbivory, Arboreal endophytes, Salicinoid, Bipartite graphics, Competition
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-150882 (URN)10.1007/s00442-018-4097-3 (DOI)000435111800016 ()29492690 (PubMedID)2-s2.0-85042590826 (Scopus ID)
Tilgjengelig fra: 2018-08-31 Laget: 2018-08-31 Sist oppdatert: 2018-08-31bibliografisk kontrollert
Randriamanana, T. R., Nissinen, K., Ovaskainen, A., Lavola, A., Peltola, H., Albrectsen, B. R. & Julkunen-Tiitto, R. (2018). Does fungal endophyte inoculation affect the responses of aspen seedlings to carbon dioxide enrichment?. Fungal ecology, 33, 24-31
Åpne denne publikasjonen i ny fane eller vindu >>Does fungal endophyte inoculation affect the responses of aspen seedlings to carbon dioxide enrichment?
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2018 (engelsk)Inngår i: Fungal ecology, ISSN 1754-5048, E-ISSN 1878-0083, Vol. 33, s. 24-31Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Endophytes are microorganisms that live inside plants without causing visible symptoms, at least during some parts of their life cycle. We studied, for the first time, the combined effects of CO2 enrichment (700 ppm) and fungal endophyte inoculation on the growth, the concentrations of low-molecular weight phenolics, and condensed tannins of aspen (Populus tremula) seedlings. As expected, we found that the endophyte strain we inoculated was neutral to plant growth and was able to bypass major plant defences. In addition, CO2 enrichment alone boosted plant growth, but had only minor effects on plant phenolics. Neither did it affect the plant-endophyte relationship. Based on our findings, we suggest that the successful and asymptomatic colonization of endophytes that we found in aspen might be due to the endophytes' special attributes enabling them to thrive inside plant tissues and to avoid or counteract the plant's chemical defences.

sted, utgiver, år, opplag, sider
Elsevier, 2018
Emneord
balanced antagonism, carbon dioxide, condensed tannins, endophytes, phenolics, Populus
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-148824 (URN)10.1016/j.funeco.2017.12.002 (DOI)000431938500003 ()2-s2.0-85042186407 (Scopus ID)
Tilgjengelig fra: 2018-06-13 Laget: 2018-06-13 Sist oppdatert: 2018-06-13bibliografisk kontrollert
Agostinelli, M., Cleary, M., Martin, J. A., Albrectsen, B. R. & Witzell, J. (2018). Pedunculate Oaks (Quercus robur L.) Differing in Vitality as Reservoirs for Fungal Biodiversity. Frontiers in Microbiology, 9, Article ID 1758.
Åpne denne publikasjonen i ny fane eller vindu >>Pedunculate Oaks (Quercus robur L.) Differing in Vitality as Reservoirs for Fungal Biodiversity
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2018 (engelsk)Inngår i: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 9, artikkel-id 1758Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Ecological significance of trees growing in urban and peri-urban settings is likely to increase in future land-use regimes, calling for better understanding of their role as potential reservoirs or stepping stones for associated biodiversity. We studied the diversity of fungal endophytes in woody tissues of asymptomatic even aged pedunculate oak trees, growing as amenity trees in a peri-urban setting. The trees were classified into three groups according to their phenotypic vitality (high, medium, and low). Endophytes were cultured on potato dextrose media from surface sterilized twigs and DNA sequencing was performed to reveal the taxonomic identity of the morphotypes. In xylem tissues, the frequency and diversity of endophytes was highest in oak trees showing reduced vitality. This difference was not found for bark samples, in which the endophyte infections were more frequent and communities more diverse than in xylem. In general, most taxa were shared across the samples with few morphotypes being recovered in unique samples. Leaf phenolic profiles were found to accurately classify the trees according to their phenotypic vitality. Our results confirm that xylem is more selective substrate for endophytes than bark and that endophyte assemblages in xylem are correlated to the degree of host vitality. Thus, high vitality of trees may be associated with reduced habitat quality to wood-associated endophytes.

Emneord
fungal diversity, endophytes, tree vitality, Quercus robur, network analysis, phenolics
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-150816 (URN)10.3389/fmicb.2018.01758 (DOI)000440694600001 ()
Forskningsfinansiär
Swedish Research Council Formas, 2012-1358
Tilgjengelig fra: 2018-08-20 Laget: 2018-08-20 Sist oppdatert: 2018-08-20bibliografisk kontrollert
Decker, V. H., Bandau, F., Gundale, M. J., Cole, C. T. & Albrectsen, B. R. (2017). Aspen phenylpropanoid genes’ expression levels correlate with genets’ tannin richness and vary both in responses to soil nitrogen and associations with phenolic profiles. Tree Physiology, 37(2), 270-279
Åpne denne publikasjonen i ny fane eller vindu >>Aspen phenylpropanoid genes’ expression levels correlate with genets’ tannin richness and vary both in responses to soil nitrogen and associations with phenolic profiles
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2017 (engelsk)Inngår i: Tree Physiology, ISSN 0829-318X, E-ISSN 1758-4469, Vol. 37, nr 2, s. 270-279Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Condensed tannin (CT) contents of European aspen (Populus tremula L.) vary among genotypes, and increases in nitrogen (N) availability generally reduce plants’ tannin production in favor of growth, through poorly understood mechanisms. We hypothesized that intrinsic tannin production rates may co-vary with gene expression responses to soil N and resource allocation within the phenylpropanoid pathway (PPP). Thus, we examined correlations between soil N levels and both expression patterns of eight PPP genes (measured by quantitative-reverse transcription PCR) and foliar phenolic compounds (measured by liquid chromatography–mass spectrometry) in young aspen genets with intrinsically extreme CT levels. Monitored phenolics included salicinoids, lignins, flavones, flavonols, CT precursors and CTs. The PPP genes were consistently expressed more strongly in high-CT trees. Low N supplements reduced expression of genes throughout the PPP in all genets, while high N doses restored expression of genes at the beginning and end of the pathway. These PPP changes were not reflected in pools of tannin precursors, but varying correlations between gene expression and foliar phenolic pools were detected in young and mature leaves, suggesting that processes linking gene expression and the resulting phenolics vary spatially and temporally. Precursor fluxes suggested that CT-related metabolic rate or sink controls are linked to intrinsic carbon allocation strategies associated with N responses. Overall, we found more negative correlations (indicative of allocation trade-offs) between PPP gene expression and phenolic products following N additions in low-CT plants than in high-CT plants. The tannin-related expression dynamics suggest that, in addition to defense, relative tannin levels may also be indicative of intraspecific variations in the way aspen genets respond to soil fertility. 

Emneord
condensed tannins, European aspen, genotypic variation, nitrogen addition, phenylpropanoid pathway, Populus tremula, salicinoids
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-126704 (URN)10.1093/treephys/tpw118 (DOI)000397052300012 ()
Tilgjengelig fra: 2016-10-13 Laget: 2016-10-13 Sist oppdatert: 2018-06-09bibliografisk kontrollert
Ponzio, C., Papazian, S., Albrectsen, B. R., Dicke, M. & Gols, R. (2017). Dual herbivore attack and herbivore density affect metabolic profiles of Brassica nigra leaves. Plant, Cell and Environment, 40(8), 1356-1367
Åpne denne publikasjonen i ny fane eller vindu >>Dual herbivore attack and herbivore density affect metabolic profiles of Brassica nigra leaves
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2017 (engelsk)Inngår i: Plant, Cell and Environment, ISSN 0140-7791, E-ISSN 1365-3040, Vol. 40, nr 8, s. 1356-1367Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Plant responses to dual herbivore attack are increasingly studied, but effects on the metabolome have largely been restricted to volatile metabolites and defence-related non-volatile metabolites. However, plants subjected to stress, such as herbivory, undergo major changes in both primary and secondary metabolism. Using a naturally occurring system, we investigated metabolome-wide effects of single or dual herbivory on Brassica nigra plants by Brevicoryne brassicae aphids and Pieris brassicae caterpillars, while also considering the effect of aphid density. Metabolomic analysis of leaf material showed that single and dual herbivory had strong effects on the plant metabolome, with caterpillar feeding having the strongest influence. Additionally, aphid-density-dependent effects were found in both the single and dual infestation scenarios. Multivariate analysis revealed treatment-specific metabolomic profiles, and effects were largely driven by alterations in the glucosinolate and sugar pools. Our work shows that analysing the plant metabolome as a single entity rather than as individual metabolites provides new insights into the subcellular processes underlying plant defence against multiple herbivore attackers. These processes appear to be importantly influenced by insect density.

Emneord
Brevicoryne brassicae, dual herbivory, induced defence, metabolomics, Pieris brassicae
HSV kategori
Forskningsprogram
biologi
Identifikatorer
urn:nbn:se:umu:diva-134649 (URN)10.1111/pce.12926 (DOI)000405275300010 ()
Tilgjengelig fra: 2017-05-10 Laget: 2017-05-10 Sist oppdatert: 2018-06-09bibliografisk kontrollert
Bandau, F., Albrectsen, B. R., Julkunen-Tiitto, R. & Gundale, M. J. (2017). Genotypic variability in Populus tremula L. affects how anthropogenic nitrogen enrichment influences litter decomposition. Plant and Soil, 410(1-2), 467-481
Åpne denne publikasjonen i ny fane eller vindu >>Genotypic variability in Populus tremula L. affects how anthropogenic nitrogen enrichment influences litter decomposition
2017 (engelsk)Inngår i: Plant and Soil, ISSN 0032-079X, E-ISSN 1573-5036, Vol. 410, nr 1-2, s. 467-481Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Boreal forests can receive substantial nitrogen (N) enrichment via atmospheric N deposition and industrial forest fertilization. While it is known that N enrichment can impact ecosystem properties, such as litter decomposition, it remains poorly understood how genetic variability within plant species modifies these impacts. We grew replicates of ten Populus tremula L. genotypes (GTs) under 3 N conditions; ambient, and levels representing atmospheric N deposition and industrial forest fertilization. We measured leaf and litter physical and chemical traits, and conducted a litter decomposition assay. Leaf traits varied due to N treatment, GT, and constitutive tannin levels. Leaf traits were in some cases correlated with litter traits, and decomposition was influenced by single and interactive effects of N and GT. Nitrogen addition unexpectedly decelerated decomposition, potentially due to changes in specific leaf area (SLA). Variation in decomposition rates among the GTs was best explained by their differences in SLA, and lignin:N ratio. Nitrogen addition also caused a shift in which traits most strongly influenced decomposition. Our findings highlight that the considerable diversity present in tree species can have a strong influence on ecosystem processes, such as decomposition, and how these processes respond to environmental change.

Emneord
Aspen, Condensed tannins, Genotypic variation, Genotype-environment interaction, Mass loss, trogen enrichment
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-131879 (URN)10.1007/s11104-016-3033-8 (DOI)000392392200035 ()
Tilgjengelig fra: 2017-02-24 Laget: 2017-02-24 Sist oppdatert: 2018-06-09bibliografisk kontrollert
Kloth, K. J., Busscher-Lange, J., Wiegers, G. L., Kruijer, W., Buijs, G., Meyer, R. C., . . . Jongsma, M. A. (2017). SIEVE ELEMENT-LINING CHAPERONE1 Restricts Aphid Feeding on Arabidopsis during Heat Stress. The Plant Cell, 29(10), 2450-2464
Åpne denne publikasjonen i ny fane eller vindu >>SIEVE ELEMENT-LINING CHAPERONE1 Restricts Aphid Feeding on Arabidopsis during Heat Stress
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2017 (engelsk)Inngår i: The Plant Cell, ISSN 1040-4651, E-ISSN 1532-298X, Vol. 29, nr 10, s. 2450-2464Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The role of phloem proteins in plant resistance to aphids is still largely elusive. By genome-wide association mapping of aphid behavior on 350 natural Arabidopsis thaliana accessions, we identified the small heat shock-like SIEVE ELEMENT-LINING CHAPERONE1 (SLI1). Detailed behavioral studies on near-isogenic and knockout lines showed that SLI1 impairs phloem feeding. Depending on the haplotype, aphids displayed a different duration of salivation in the phloem. On sli1 mutants, aphids prolonged their feeding sessions and ingested phloem at a higher rate than on wild-type plants. The largest phenotypic effects were observed at 26 degrees C, when SLI1 expression is upregulated. At this moderately high temperature, sli1 mutants suffered from retarded elongation of the inflorescence and impaired silique development. Fluorescent reporter fusions showed that SLI1 is confined to the margins of sieve elements where it lines the parietal layer and colocalizes in spherical bodies around mitochondria. This localization pattern is reminiscent of the clamp-like structures observed in previous ultrastructural studies of the phloem and shows that the parietal phloem layer plays an important role in plant resistance to aphids and heat stress.

sted, utgiver, år, opplag, sider
AMER SOC PLANT BIOLOGISTS, 2017
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-142272 (URN)10.1105/tpc.16.00424 (DOI)000414861100015 ()
Tilgjengelig fra: 2017-11-30 Laget: 2017-11-30 Sist oppdatert: 2018-06-09bibliografisk kontrollert
Organisasjoner
Identifikatorer
ORCID-id: ORCID iD iconorcid.org/0000-0002-9337-4540