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Talsi, O., Berggren, R. K., Johansson, G. & Winsö, O. (2019). A national survey on routines regarding sedation in Swedish intensive care units. Upsala Journal of Medical Sciences, 37(23), 3088-3096
Öppna denna publikation i ny flik eller fönster >>A national survey on routines regarding sedation in Swedish intensive care units
2019 (Engelska)Ingår i: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 37, nr 23, s. 3088-3096Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Previous studies concerning sedation in Swedish intensive care units (ICU) have shown variability in drug choices and strategies. Currently, there are no national guidelines on this topic. As an update to a Nordic survey from 2004, and as a follow-up to a recently introduced quality indicator from the Swedish Intensive Care Registry, we performed a national survey.

Methods: A digital survey was sent to the ICUs in Sweden, asking for sedation routines regarding hypnosedatives, analgosedatives, protocols, sedation scales, etc.

Results: Fifty out of 80 ICUs responded to the survey. All units used sedation scales, and 88% used the RASS scale; 80% used written guidelines for sedation. Propofol and dexmedetomidine were the preferred short-term hypnosedatives. Propofol, dexmedetomidine, and midazolam were preferred for long-term hypnosedation. Remifentanil, morphine, and fentanyl were the most frequently used agents for analgosedation.

Conclusions: All ICUs used a sedation scale, an increase compared with previous studies. Concerning the choice of hypno- and analgosedatives, the use of dexmedetomidine, clonidine, and remifentanil has increased, and the use of benzodiazepines has decreased since the Nordic survey in 2004.

Ort, förlag, år, upplaga, sidor
Taylor & Francis, 2019
Nyckelord
Analgesics, intensive care, mechanical ventilation, sedation scale, sedatives
Nationell ämneskategori
Anestesi och intensivvård
Identifikatorer
urn:nbn:se:umu:diva-160299 (URN)10.1080/03009734.2019.1616339 (DOI)000469543300001 ()31119971 (PubMedID)
Tillgänglig från: 2019-06-17 Skapad: 2019-06-17 Senast uppdaterad: 2019-06-17Bibliografiskt granskad
Åkesson, O., Falkenback, D., Johansson, G. & Abrahamsson, P. (2019). Surface Microdialysis Detects Ischemia After Esophageal Resection: An Experimental Animal Study. Journal of Surgical Research, 245, 537-543
Öppna denna publikation i ny flik eller fönster >>Surface Microdialysis Detects Ischemia After Esophageal Resection: An Experimental Animal Study
2019 (Engelska)Ingår i: Journal of Surgical Research, ISSN 0022-4804, E-ISSN 1095-8673, Vol. 245, s. 537-543Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

BACKGROUND: After an esophageal resection, continuity is commonly restored by a gastric tube reconstruction and an intrathoracic anastomosis to the remaining proximal esophagus. Ischemia of the anastomotic region is considered to play a pivotal role in anastomotic leakage. Microdialysis (μD) is an excellent method to measure local biochemical substances and parameters in a specific organ or compartment aiming at early detection of ischemia. This animal study evaluates ischemia of the gastric tube reconstruction using a novel method-μD on organ surfaces. This promising method may have the potential to detect an anastomotic leakage before clinical symptoms develop.

METHODS: Anesthetized normoventilated pigs were used. Surface microdialysis (S-μD) catheters and an intraparenchymal oxygen tension catheter were placed on the stomach. A gastric tube was made and the gastroepiploic artery was divided halfway along the greater curvature to produce severe ischemia at the top of the gastric tube. μD data from four locations (gastric tube, ileum and peritoneal cavity) were recorded every 20 min during the experiment. Tissue samples from all catheter sites underwent histopathological analysis. Intraparenchymal oxygen partial pressure, systemic blood tests, and hemodynamic parameters were recorded.

RESULTS: S-μD data showed values indicating severe ischemia at the top of the gastric tube and intermediate ischemia at the level of transection of the gastroepiploic artery. Ischemia was verified by histopathological analysis of tissue samples and intraparenchymal oxygen tension data.

CONCLUSIONS: S-μD can detect and grade severity of local ischemia in real time, in an animal model.

Ort, förlag, år, upplaga, sidor
Elsevier, 2019
Nyckelord
Ischemia, Metabolism, Microdialysis
Nationell ämneskategori
Kirurgi Anestesi och intensivvård Gastroenterologi
Identifikatorer
urn:nbn:se:umu:diva-162922 (URN)10.1016/j.jss.2019.07.060 (DOI)31470334 (PubMedID)
Tillgänglig från: 2019-09-02 Skapad: 2019-09-02 Senast uppdaterad: 2019-09-03Bibliografiskt granskad
Winsö, O., Kral, J., Wang, W., Kralova, I., Abrahamsson, P., Johansson, G. & Blind, P.-J. (2018). Thoracic epidural anaesthesia reduces insulin resistance and inflammatory response in experimental acute pancreatitis. Upsala Journal of Medical Sciences, 123(4), 207-215
Öppna denna publikation i ny flik eller fönster >>Thoracic epidural anaesthesia reduces insulin resistance and inflammatory response in experimental acute pancreatitis
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2018 (Engelska)Ingår i: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 123, nr 4, s. 207-215Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

AIMS: The activity of the sympathetic nervous system (SNS) is crucial at an early stage in the development of an inflammatory reaction. A study of metabolic events globally and locally in the early phase of acute pancreatitis (AP), implying hampered SNS activity, is lacking. We hypothesized that thoracic epidural anaesthesia (TEA) modulates the inflammatory response and alleviates the severity of AP in pigs.

MATERIAL AND METHODS: The taurocholate (TC) group (n = 8) had only TC AP. The TC + TEA group (n = 8) had AP and TEA. A control group (n = 8) underwent all the preparations, without having AP or TEA. Metabolic changes in the pancreas were evaluated by microdialysis and by histopathological examination.

RESULTS: The relative increase in serum lipase concentrations was more pronounced in the TC group than in TC + TEA and control groups. A decrease in relative tissue oxygen tension (PtiO2) levels occurred one hour later in the TC + TEA group than in the TC group. The maintenance of normoglycaemia in the TC group required a higher glucose infusion rate than in the TC + TEA group. The relative decrease in serum insulin concentrations was most pronounced in the TC + TEA group.

CONCLUSION: TEA attenuates the development of AP, as indicated by changes observed in haemodynamic parameters and by the easier maintenance of glucose homeostasis. Further, TEA was associated with attenuated insulin resistance and fewer local pathophysiological events.

Ort, förlag, år, upplaga, sidor
Abingdon: Taylor & Francis, 2018
Nyckelord
Acute pancreatitis, epidural anaesthesia, insulin, microdialysis, sodium-taurocholic acid, sympathetic nervous system
Nationell ämneskategori
Anestesi och intensivvård Kirurgi
Identifikatorer
urn:nbn:se:umu:diva-153672 (URN)10.1080/03009734.2018.1539054 (DOI)000455702800003 ()30468105 (PubMedID)
Tillgänglig från: 2018-11-26 Skapad: 2018-11-26 Senast uppdaterad: 2019-02-25Bibliografiskt granskad
Jacobsson, S., Larsson, P., Johansson, G., Norberg, M., Wadell, G., Hallmans, G., . . . Söderberg, S. (2017). Leptin independently predicts development of sepsis and its outcome. Journal of Inflammation, 14, Article ID 19.
Öppna denna publikation i ny flik eller fönster >>Leptin independently predicts development of sepsis and its outcome
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2017 (Engelska)Ingår i: Journal of Inflammation, ISSN 1476-9255, E-ISSN 1476-9255, Vol. 14, artikel-id 19Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Sepsis is a life-threatening condition and obesity is related to the clinical outcome. The underlying reasons are incompletely understood, but the adipocyte derived hormones leptin and adiponectin may be involved.

Methods: Patients aged 18 years or more with documented first time sepsis events were included in a nested case-referent study if they had participated in previous health surveys. Two matched referents free of known sepsis were identified. Circulating levels of leptin and adiponectin were determined in stored plasma, and their impact on a future sepsis event and its outcome was evaluated.

Results: We identified 152 patients (62% women) with a sepsis event and a previous participation in a health survey. Eighty-three % had also blood samples from the acute event. Hyperleptinemia at health survey associated with a future sepsis event (OR 1.77, 95% CI 1.04-3.00) and with hospital death. After adjustment for BMI leptin remained associated with sepsis in men, but not in women. High levels in the acute phase associated with increased risk for in hospital death in women (OR 4.18, 95% CI 1.17-15.00), while being protective in men (OR 0.05, 95% CI 0.01-0.48). Furthermore, leptin increased more from baseline to the acute phase in men than in women. Adiponectin did not predict sepsis and did not relate to outcome.

Conclusions: Hyperleptinemia independently predicted the development of sepsis and an unfavourable outcome in men, and inertia in the acute response related to worse outcome.

Ort, förlag, år, upplaga, sidor
London: BioMed Central, 2017
Nyckelord
Sepsis, Leptin, Adiponectin, Obesity, Case-referent study, Sex
Nationell ämneskategori
Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi
Identifikatorer
urn:nbn:se:umu:diva-140039 (URN)10.1186/s12950-017-0167-2 (DOI)000410649100001 ()28919840 (PubMedID)
Tillgänglig från: 2017-10-05 Skapad: 2017-10-05 Senast uppdaterad: 2019-05-23Bibliografiskt granskad
Brändström, H., Sundelin, A., Hoseason, D., Sundström, N., Birgander, R., Johansson, G., . . . Haney, M. (2017). Risk for intracranial pressure increase related to enclosed air in post-craniotomy patients during air ambulance transport: a retrospective cohort study with simulation. Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, 25, Article ID 50.
Öppna denna publikation i ny flik eller fönster >>Risk for intracranial pressure increase related to enclosed air in post-craniotomy patients during air ambulance transport: a retrospective cohort study with simulation
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2017 (Engelska)Ingår i: Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, ISSN 1757-7241, E-ISSN 1757-7241, Vol. 25, artikel-id 50Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

BACKGROUND: Post-craniotomy intracranial air can be present in patients scheduled for air ambulance transport to their home hospital. We aimed to assess risk for in-flight intracranial pressure (ICP) increases related to observed intracranial air volumes, hypothetical sea level pre-transport ICP, and different potential flight levels and cabin pressures. METHODS: A cohort of consecutive subdural hematoma evacuation patients from one University Medical Centre was assessed with post-operative intracranial air volume measurements by computed tomography. Intracranial pressure changes related to estimated intracranial air volume effects of changing atmospheric pressure (simulating flight and cabin pressure changes up to 8000 ft) were simulated using an established model for intracranial pressure and volume relations. RESULTS: Approximately one third of the cohort had post-operative intracranial air. Of these, approximately one third had intracranial air volumes less than 11 ml. The simulation estimated that the expected changes in intracranial pressure during 'flight' would not result in intracranial hypertension. For intracranial air volumes above 11 ml, the simulation suggested that it was possible that intracranial hypertension could develop 'inflight' related to cabin pressure drop. Depending on the pre-flight intracranial pressure and air volume, this could occur quite early during the assent phase in the flight profile. DISCUSSION: These findings support the idea that there should be radiographic verification of the presence or absence of intracranial air after craniotomy for patients planned for long distance air transport. CONCLUSIONS: Very small amounts of air are clinically inconsequential. Otherwise, air transport with maintained ground-level cabin pressure should be a priority for these patients.

Ort, förlag, år, upplaga, sidor
BioMed Central, 2017
Nyckelord
Air ambulance, Intracranial pressure, Pneumocephalus
Nationell ämneskategori
Anestesi och intensivvård Neurovetenskaper Kirurgi Radiologi och bildbehandling
Identifikatorer
urn:nbn:se:umu:diva-134974 (URN)10.1186/s13049-017-0394-9 (DOI)000401225800001 ()28499454 (PubMedID)
Tillgänglig från: 2017-05-15 Skapad: 2017-05-15 Senast uppdaterad: 2018-06-09Bibliografiskt granskad
Axelsson, B., Häggmark, S., Svenmarker, S., Johansson, G., Gupta, A., Tyden, H., . . . Haney, M. (2016). Effects of Combined Milrinone and Levosimendan Treatment on Systolic and Diastolic Function During Postischemic Myocardial Dysfunction in a Porcine Model. Journal of Cardiovascular Pharmacology and Therapeutics, 21(5), 495-503
Öppna denna publikation i ny flik eller fönster >>Effects of Combined Milrinone and Levosimendan Treatment on Systolic and Diastolic Function During Postischemic Myocardial Dysfunction in a Porcine Model
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2016 (Engelska)Ingår i: Journal of Cardiovascular Pharmacology and Therapeutics, ISSN 1074-2484, E-ISSN 1940-4034, Vol. 21, nr 5, s. 495-503Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

It is not known whether there are positive or negative interactions on ventricular function when a calcium-sensitizing inotrope is added to a phosphodiesterase inhibitor in the clinical setting of acute left ventricular (LV) dysfunction. We hypothesized that when levosimendan is added to milrinone treatment, there will be synergetic inotropic and lusitropic effects. This was tested in an anesthetized porcine postischemic global LV injury model, where ventricular pressures and volumes (conductance volumetry) were measured. A global ischemic injury was induced by repetitive left main stem coronary artery occlusions. Load-independent indices of LV function were assessed before and after ventricular injury, after milrinone treatment, and finally after addition of levosimendan to the milrinone treatment. Nonparametric, within-group comparisons were made. The protocol was completed in 12 pigs, 7 of which received the inotrope treatment and 5 of which served as controls. Milrinone led to positive lusitropic effects seen by improvement in tau after myocardial stunning. The addition of levosimendan to milrinone further increased lusitropic state. The latter effect could however not be attributed solely to levosimendan, since lusitropic state also improved spontaneously in time-matched controls at the same rate during the corresponding period. When levosimendan was added to milrinone infusion, there was no increase in systolic function (preload recruitable stroke work) compared to milrinone treatment alone. We conclude that in this model of postischemic LV dysfunction, there appears to be no clear improvement in systolic or diastolic function after addition of levosimendan to established milrinone treatment but also no negative effects of levosimendan in this context.

Ort, förlag, år, upplaga, sidor
Sage Publications, 2016
Nyckelord
cardiac pharmacology, cardioactive agents, experimental and clinical heart failure, ischemia-reperfusion injury
Nationell ämneskategori
Anestesi och intensivvård Kardiologi
Identifikatorer
urn:nbn:se:umu:diva-116180 (URN)10.1177/1074248416628675 (DOI)000382567800008 ()26837238 (PubMedID)1940-4034 (Electronic) 1074-2484 (Linking) (ISBN)
Tillgänglig från: 2016-02-09 Skapad: 2016-02-09 Senast uppdaterad: 2019-05-22Bibliografiskt granskad
Abrahamsson, P., Johansson, G., Åberg, A.-M., Winsö, O. & Blind, P. J. (2016). Outcome of microdialysis sampling on liver surface and parenchyma. Journal of Surgical Research, 200(2), 480-487
Öppna denna publikation i ny flik eller fönster >>Outcome of microdialysis sampling on liver surface and parenchyma
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2016 (Engelska)Ingår i: Journal of Surgical Research, ISSN 0022-4804, E-ISSN 1095-8673, Vol. 200, nr 2, s. 480-487Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: To investigate whether surface microdialysis (μD) sampling in probes covered by a plastic film, as compared to noncovered and to intraparenchymatous probes, would increase the technique's sensitivity for pathophysiologic events occurring in a liver ischemia-reperfusion model. Placement of μD probes in the parenchyma of an organ, as is conventionally done, may cause adverse effects, e.g., bleeding, possibly influencing outcome.

Methods: A transient ischemia-reperfusion model of the liver was used in six anesthetized normoventilated pigs. μD probes were placed in the parenchyma and on the liver surface. Surface probes were either left uncovered or were covered by plastic film.

Results: Lactate and glucose levels were significantly higher in plastic film covered probes than in uncovered surface probes throughout the ischemic period. Glycerol levels were significantly higher in plastic film covered probes than in uncovered surface probes at 30 and 45 min into ischemia.

Conclusions: Covering the μD probe increases the sensibility of the μD–technique in monitoring an ischemic insult and reperfusion in the liver. These findings confirm that the principle of surface μD works, possibly replacing need of intraparenchymatous placement of μD probes. Surface μD seemingly allows, noninvasively from an organ's surface, via the extracellular compartment, assessment of intracellular metabolic events. The finding that covered surface μD probes allows detection of local metabolic changes earlier than do intraparenchymatous probes, merit further investigation focusing on μD probe design.

Nyckelord
Microdialysis, Liver, Ischemia, Reperfusion, Surface probe, Metabolism
Nationell ämneskategori
Fysiologi Biomedicinsk laboratorievetenskap/teknologi Kirurgi
Identifikatorer
urn:nbn:se:umu:diva-112153 (URN)10.1016/j.jss.2015.09.009 (DOI)000366841500010 ()26505659 (PubMedID)
Tillgänglig från: 2015-12-03 Skapad: 2015-12-03 Senast uppdaterad: 2018-06-07Bibliografiskt granskad
Åkesson, O., Abrahamsson, P., Johansson, G. & Blind, P.-J. (2016). Surface microdialysis on small bowel serosa in monitoring of ischemia. Journal of Surgical Research, 204(1), 39-46
Öppna denna publikation i ny flik eller fönster >>Surface microdialysis on small bowel serosa in monitoring of ischemia
2016 (Engelska)Ingår i: Journal of Surgical Research, ISSN 0022-4804, E-ISSN 1095-8673, Vol. 204, nr 1, s. 39-46Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Ischemic injury of an organ causes metabolic change from aerobic to anaerobic metabolism. It has been shown in experimental studies on the heart and liver that such conversion may be detected by conventional microdialysis probes placed intraparenchymatously, as well as on organ surfaces, by assaying lactate, pyruvate, glucose, and glycerol in dialysate. We developed a microdialysis probe (S-mu D) intended for use solely on organ surfaces. The aim of this study was to assess whether the newly developed S-mu D probe could be used for detection and monitoring of small bowel ischemia. Methods: In anesthetized normoventilated pigs, a control S-mu D probe was applied on the jejunal serosa 50 cm downstream from the duodenojejunal junction (DJJ). Starting 100 cm from DJJ, a 100-cm long ischemic segment was created by division of all mesenteric vessels. S-mu Ds were applied at 2.5, 5, 20, and 50 cm from the starting point of ischemia by serosal sutures. A standard mu D probe was placed in the abdominal cavity as a further control. Dialysate was harvested before inducing ischemia and subsequently every 20 min for 4 h. Central venous blood was drawn every hour to monitor systemic lactate, C-reactive protein, and white blood cell count. Results: Microdialysis lactate levels were significantly higher than baseline from 20 min on into protocol time in the ischemic segment and in the control S-mu D probe. The peritoneal cavity probe showed no significant elevation. Lactate levels from the ischemic segment reached a plateau at 60 min. Courses of pyruvate, glucose, and glycerol levels were in accordance with transition from an aerobic to anaerobic metabolism in the bowel wall. No statistically significant changes in hemoglobin, white blood cell count, or lactate values in central venous blood were recorded. Conclusions: Assaying the aforementioned compounds in dialysate, harvested by the newly developed S-mu D probe, allowed detection and monitoring of small bowel ischemia from 20 min on following its onset.

Nyckelord
Surface microdialysis, Bowel, Ischemia, Pig, Experimental surgery
Nationell ämneskategori
Kirurgi Anestesi och intensivvård Gastroenterologi
Identifikatorer
urn:nbn:se:umu:diva-125597 (URN)10.1016/j.jss.2016.04.001 (DOI)000380750000008 ()27451866 (PubMedID)
Tillgänglig från: 2016-09-13 Skapad: 2016-09-13 Senast uppdaterad: 2018-06-07Bibliografiskt granskad
Brändström, H., Johansson, G., Giesbrecht, G. G., Ängquist, K.-A. & Haney, M. F. (2014). Accidental cold-related injury leading to hospitalization in northern Sweden: an eight-year retrospective analysis. Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, 22, 6
Öppna denna publikation i ny flik eller fönster >>Accidental cold-related injury leading to hospitalization in northern Sweden: an eight-year retrospective analysis
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2014 (Engelska)Ingår i: Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, ISSN 1757-7241, E-ISSN 1757-7241, Vol. 22, s. 6-Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Cold injuries are rare but important causes of hospitalization. We aimed to identify the magnitude of cold injury hospitalization, and assess causes, associated factors and treatment routines in a subarctic region. Methods: In this retrospective analysis of hospital records from the 4 northernmost counties in Sweden, cases from 2000-2007 were identified from the hospital registry by diagnosis codes for accidental hypothermia, frostbite, and cold-water drowning.Results were analyzed for pre-hospital site events, clinical events in-hospital, and complications observed with mild (temperature 34.9 - 32 degrees C), moderate (31.9 - 28 degrees C) and severe (<28 degrees C), hypothermia as well as for frostbite and cold-water drowning. Results: From the 362 cases, average annual incidences for hypothermia, frostbite, and cold-water drowning were estimated to be 3.4/100 000, 1.5/100 000, and 0.8/100 000 inhabitants, respectively. Annual frequencies for hypothermia hospitalizations increased by approximately 3 cases/year during the study period. Twenty percent of the hypothermia cases were mild, 40% moderate, and 24% severe. For 12%, the lowest documented core temperature was 35 degrees C or higher, for 4% there was no temperature documented. Body core temperature was seldom measured in pre-hospital locations. Of 362 cold injury admissions, 17 (5%) died in hospital related to their injuries. Associated co-factors and co-morbidities included ethanol consumption, dementia, and psychiatric diagnosis. Conclusions: The incidence of accidental hypothermia seems to be increasing in this studied sub-arctic region. Likely associated factors are recognized (ethanol intake, dementia, and psychiatric diagnosis).

Ort, förlag, år, upplaga, sidor
BioMed Central, 2014
Nyckelord
accidental hypothermia, frostbite, body temperature, rewarming, cold-water drowning
Nationell ämneskategori
Anestesi och intensivvård
Identifikatorer
urn:nbn:se:umu:diva-89251 (URN)10.1186/1757-7241-22-6 (DOI)000334792400001 ()
Tillgänglig från: 2014-05-26 Skapad: 2014-05-26 Senast uppdaterad: 2018-06-07Bibliografiskt granskad
Svennerholm, K., Bergh, N., Larsson, P., Jern, S., Johansson, G., Biber, B. & Haney, M. (2014). Histone Deacetylase Inhibitor Treatment Increases Coronary t-PA Release in a Porcine Ischemia Model. PLoS ONE, 9(5), e97260
Öppna denna publikation i ny flik eller fönster >>Histone Deacetylase Inhibitor Treatment Increases Coronary t-PA Release in a Porcine Ischemia Model
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2014 (Engelska)Ingår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 9, nr 5, s. e97260-Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

BACKGROUND: The expression of the tissue plasminogen activator gene can be affected by histone deacetylation inhibition and thus appears to be under epigenetic control.

OBJECTIVES: The study aimed to test if in vivo pharmacological intervention by valproic acid treatment would lead to increase in tissue plasminogen activator release capacity. METHODS: In an anaesthetized pig model, a controlled transient coronary occlusion was used to stimulate coronary tissue plasminogen activator release in a valproic acid treated (one week) and a non-treated group. Coronary venous blood samples from the ischemic region were collected, great cardiac vein thermodilution flow measurements were performed, and trans-coronary tissue plasminogen activator fluxes were calculated. Plasminogen activator inhibitor-1 was also measured.

RESULTS: Adequate sampling from the affected area after the 10 minute ischemic period was confirmed by lactate measurements. Fluxes for tissue plasminogen activator at minutes 1, 3, 5, 7 and 10 were measured and then used to present cumulative net tissue plasminogen activator release for the whole measurement period for both groups. Area under the curve was higher for the valproic acid treated group at 10 minutes; 932+/-173 nanograms (n = 12) compared to the non-treated group, 451+/-78 nanograms (n = 10, p = 0.023). There was no difference in levels of plasminogen activator inhibitor-1 between groups.

CONCLUSIONS: These findings support a proof of concept for histone deacetylation inhibition positive effect on tissue plasminogen activator expression in an in vivo setting. Further studies are needed to find an optimal way to implement histone deacetylation inhibition to achieve desired clinical changes in tissue plasminogen activator expression.

Nationell ämneskategori
Anestesi och intensivvård
Identifikatorer
urn:nbn:se:umu:diva-89079 (URN)10.1371/journal.pone.0097260 (DOI)000336653300099 ()24818610 (PubMedID)
Tillgänglig från: 2014-05-21 Skapad: 2014-05-21 Senast uppdaterad: 2018-06-07Bibliografiskt granskad
Organisationer
Identifikatorer
ORCID-id: ORCID iD iconorcid.org/0000-0002-5325-2688

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