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Thornell, Lars-Eric
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Thornell, L.-E., Carlsson, L., Eriksson, P.-O., Liu, J.-X., Österlund, C., Stål, P. & Pedrosa-Domellöf, F. (2015). Fibre typing of intrafusal fibres. Journal of Anatomy, 227(2), 136-156
Öppna denna publikation i ny flik eller fönster >>Fibre typing of intrafusal fibres
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2015 (Engelska)Ingår i: Journal of Anatomy, ISSN 0021-8782, E-ISSN 1469-7580, Vol. 227, nr 2, s. 136-156Artikel, forskningsöversikt (Refereegranskat) Published
Abstract [en]

The first descriptions of muscle spindles with intrafusal fibres containing striated myofibrils and nervous elements were given approximately 150years ago. It took, however, another 100years to establish the presence of two types of intrafusal muscle fibres: nuclear bag and nuclear chain fibres. The present paper highlights primarily the contribution of Robert Banks in fibre typing of intrafusal fibres: the confirmation of the principle of two types of nuclear bag fibres in mammalian spindles and the variation in occurrence of a dense M-band along the fibres. Furthermore, this paper summarizes how studies from the Umea University group (Laboratory of Muscle Biology in the Department of Integrative Medical Biology) on fibre typing and the structure and composition of M-bands have contributed to the current understanding of muscle spindle complexity in adult humans as well as to muscle spindle development and effects of ageing. The variable molecular composition of the intrafusal sarcomeres with respect to myosin heavy chains and M-band proteins gives new perspectives on the role of the intrafusal myofibrils as stretch-activated sensors influencing tension/stiffness and signalling to nuclei.

Ort, förlag, år, upplaga, sidor
John Wiley & Sons, 2015
Nyckelord
cytoskeleton, M-band, M-protein, muscle spindle, myomesin, nuclear bag, nuclear chain, titin
Nationell ämneskategori
Annan biologi
Identifikatorer
urn:nbn:se:umu:diva-106768 (URN)10.1111/joa.12338 (DOI)000357951900004 ()26179023 (PubMedID)
Tillgänglig från: 2015-08-20 Skapad: 2015-08-07 Senast uppdaterad: 2018-06-07Bibliografiskt granskad
Österlund, C., Liu, J.-X., Thornell, L.-E. & Eriksson, P.-O. (2013). Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences. Histochemistry and Cell Biology, 139(6), 895-907
Öppna denna publikation i ny flik eller fönster >>Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences
2013 (Engelska)Ingår i: Histochemistry and Cell Biology, ISSN 0948-6143, E-ISSN 1432-119X, Vol. 139, nr 6, s. 895-907Artikel i tidskrift (Övrigt vetenskapligt) Published
Abstract [en]

Muscle spindles are skeletal muscle mechanoreceptors that provide proprioceptive information to the central nervous system. The human adult masseter muscle has greater number, larger and more complex muscle spindles than the adult biceps. For a better knowledge of muscle diversity and physiological properties, this study examined the myosin heavy chain (MyHC) expression of muscle spindle intrafusal fibres in the human young masseter and young biceps muscles by using a panel of monoclonal antibodies (mAbs) against different MyHC isoforms. Eight MyHC isoforms were detected in both muscles-slow-tonic, I, IIa, IIx, foetal, embryonic, α-cardiac and an isoform not previously reported in intrafusal fibres, termed IIx'. Individual fibres co-expressed 2-6 isoforms. MyHC-slow tonic separated bag(1), AS-bag(1) and bag(2) fibres from chain fibres. Typically, bag fibres also expressed MyHC-I and α-cardiac, whereas chain fibres expressed IIa and foetal. In the young masseter 98 % of bag(1) showed MyHC-α cardiac versus 30 % in the young biceps, 35 % of bag(2) showed MyHC-IIx' versus none in biceps, 17 % of the chain fibres showed MyHC-I versus 61 % in the biceps. In conclusion, the result showed fundamental similarities in intrafusal MyHC expression between young masseter and biceps, but also marked differences implying muscle-specific proprioceptive control, probably related to diverse evolutionary and developmental origins. Finding of similarities in MyHC expression between young and adult masseter and biceps muscle spindles, respectively, in accordance with previously reported similarities in mATPase fibre type composition suggest early maturation of muscle spindles, preceding extrafusal fibres in growth and maturation.

Ort, förlag, år, upplaga, sidor
Springer, 2013
Nyckelord
Jaw, Limb, Human, Muscle spindles, Intrafusal fibres, Myosin heavy chain, Immuno-histochemistry
Nationell ämneskategori
Odontologi
Identifikatorer
urn:nbn:se:umu:diva-46771 (URN)10.1007/s00418-012-1072-7 (DOI)000319167800008 ()23306907 (PubMedID)
Tillgänglig från: 2011-09-13 Skapad: 2011-09-13 Senast uppdaterad: 2018-06-08Bibliografiskt granskad
Yu, J.-G., Liu, J.-X., Carlsson, L., Thornell, L.-E. & Stål, P. S. (2013). Re-evaluation of sarcolemma injury and muscle swelling in human skeletal muscles after eccentric exercise. PLoS ONE, 8(4), Article ID e62056.
Öppna denna publikation i ny flik eller fönster >>Re-evaluation of sarcolemma injury and muscle swelling in human skeletal muscles after eccentric exercise
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2013 (Engelska)Ingår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, nr 4, artikel-id e62056Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The results regarding the effects of unaccustomed eccentric exercise on muscle tissue are often conflicting and the aetiology of delayed onset muscle soreness (DOMS) induced by eccentric exercise is still unclear. This study aimed to re-evaluate the paradigm of muscular alterations with regard to muscle sarcolemma integrity and fibre swelling in human muscles after voluntary eccentric exercise leading to DOMS. Ten young males performed eccentric exercise by downstairs running. Biopsies from the soleus muscle were obtained from 6 non-exercising controls, 4 exercised subjects within 1 hour and 6 exercised subjects at 2-3 days and 7-8 days after the exercise. Muscle fibre sarcolemma integrity, infiltration of inflammatory cells and changes in fibre size and fibre phenotype composition as well as capillary supply were examined with specific antibodies using enzyme histochemistry and immunohistochemistry. Although all exercised subjects experienced DOMS which peaked between 1.5 to 2.5 days post exercise, no significant sarcolemma injury or inflammation was detected in any post exercise group. The results do not support the prevailing hypothesis that eccentric exercise causes an initial sarcolemma injury which leads to subsequent inflammation after eccentric exercise. The fibre size was 24% larger at 7-8 days than at 2-3 days post exercise (p<0.05). In contrast, the value of capillary number per fibre area tended to decrease from 2-3 days to 7-8 days post exercise (lower in 5 of the 6 subjects at 7-8 days than at 2-3 days; p<0.05). Thus, the increased fibre size at 7-8 days post exercise was interpreted to reflect fibre swelling. Because the fibre swelling did not appear at the time that DOMS peaked (between 1.5 to 2.5 days post exercise), we concluded that fibre swelling in the soleus muscle is not directly associated with the symptom of DOMS.

Nationell ämneskategori
Idrottsvetenskap
Identifikatorer
urn:nbn:se:umu:diva-68821 (URN)10.1371/journal.pone.0062056 (DOI)000317563300048 ()23614012 (PubMedID)
Tillgänglig från: 2013-04-25 Skapad: 2013-04-25 Senast uppdaterad: 2018-06-08Bibliografiskt granskad
Dardevet, D., Savary-Auzeloux, I., Remond, D., Mosoni, L., Marzetti, E., Buford, T. W., . . . Ulfhake, B. (2012). Commentaries on Viewpoint: muscle atrophy is not always sarcopenia.. Journal of applied physiology, 113(4), 680-684
Öppna denna publikation i ny flik eller fönster >>Commentaries on Viewpoint: muscle atrophy is not always sarcopenia.
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2012 (Engelska)Ingår i: Journal of applied physiology, ISSN 8750-7587, E-ISSN 1522-1601, Vol. 113, nr 4, s. 680-684Artikel i tidskrift, Editorial material (Refereegranskat) Published
Ort, förlag, år, upplaga, sidor
American Physiological Society, 2012
Nationell ämneskategori
Medicinska och farmaceutiska grundvetenskaper
Identifikatorer
urn:nbn:se:umu:diva-82679 (URN)10.1152/japplphysiol.00667.2012 (DOI)22896680 (PubMedID)
Tillgänglig från: 2013-11-06 Skapad: 2013-11-06 Senast uppdaterad: 2018-06-08Bibliografiskt granskad
Österlund, C., Lindström, M., Thornell, L.-E. & Eriksson, P.-O. (2012). Remarkable heterogeneity in myosin heavy-chain composition of the human young masseter compared with young biceps brachii. Histochemistry and Cell Biology, 138(4), 669-682
Öppna denna publikation i ny flik eller fönster >>Remarkable heterogeneity in myosin heavy-chain composition of the human young masseter compared with young biceps brachii
2012 (Engelska)Ingår i: Histochemistry and Cell Biology, ISSN 0948-6143, E-ISSN 1432-119X, Vol. 138, nr 4, s. 669-682Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Adult human jaw muscles differ from limb and trunk muscles in enzyme-histochemical fibre type composition. Recently, we showed that the human masseter and biceps differ in fibre type pattern already at childhood. The present study explored the myosin heavy-chain (MyHC) expression in the young masseter and biceps muscles by means of gel electrophoresis (GE) and immuno-histochemical (IHC) techniques. Plasticity in MyHC expression during life was evaluated by comparing the results with the previously reported data for adult muscles. In young masseter, GE identified MyHC-I, MyHC-IIa MyHC-IIx and small proportions of MyHC-fetal and MyHC-alpha cardiac. Western blots confirmed the presence of MyHC-I, MyHC-IIa and MyHC-IIx. IHC revealed in the masseter six isomyosins, MyHC-I, MyHC-IIa, MyHC-IIx, MyHC-fetal, MyHC alpha-cardiac and a previously not reported isoform, termed MyHC-IIx'. The majority of the masseter fibres co-expressed two to four isoforms. In the young biceps, both GE and IHC identified MyHC-I, MyHC-IIa and MyHC-IIx. MyHC-I predominated in both muscles. Young masseter showed more slow and less-fast and fetal MyHC than the adult and elderly masseter. These results provide evidence that the young masseter muscle is unique in MyHC composition, expressing MyHC-alpha cardiac and MyHC-fetal isoforms as well as hitherto unrecognized potential spliced isoforms of MyHC-fetal and MyHC-IIx. Differences in masseter MyHC expression between young adult and elderly suggest a shift from childhood to adulthood towards more fast contractile properties. Differences between masseter and biceps are proposed to reflect diverse evolutionary and developmental origins and confirm that the masseter and biceps present separate allotypes of muscle.

Nationell ämneskategori
Odontologi
Identifikatorer
urn:nbn:se:umu:diva-46738 (URN)10.1007/s00418-012-0985-5 (DOI)
Tillgänglig från: 2011-09-13 Skapad: 2011-09-13 Senast uppdaterad: 2018-06-08Bibliografiskt granskad
Thornell, L.-E., Gustafsson, T., Cederholm, T. & Ulfhake, B. (2012). "Sarcopenia" has earned its living [Letter to the editor]. Journal of applied physiology, 113(4), 683-684
Öppna denna publikation i ny flik eller fönster >>"Sarcopenia" has earned its living
2012 (Engelska)Ingår i: Journal of applied physiology, ISSN 8750-7587, E-ISSN 1522-1601, Vol. 113, nr 4, s. 683-684Artikel i tidskrift, Letter (Refereegranskat) Published
Ort, förlag, år, upplaga, sidor
American Physiological Society, 2012
Nationell ämneskategori
Idrottsvetenskap Fysiologi
Identifikatorer
urn:nbn:se:umu:diva-60340 (URN)000308000800029 ()
Tillgänglig från: 2013-01-03 Skapad: 2012-10-09 Senast uppdaterad: 2018-06-08Bibliografiskt granskad
Österlund, C., Thornell, L.-E. & Eriksson, P.-O. (2011). Differences in fibre type composition between human masseter and biceps muscles in young and adults reveal unique masseter fibre type growth pattern. Anatomical Record, 294(7), 1158-1169
Öppna denna publikation i ny flik eller fönster >>Differences in fibre type composition between human masseter and biceps muscles in young and adults reveal unique masseter fibre type growth pattern
2011 (Engelska)Ingår i: Anatomical Record, ISSN 0003-276X, E-ISSN 1097-0185, Vol. 294, nr 7, s. 1158-1169Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The human jaw system is different from those of other primates, carnivores, ruminants, and rodents in temporomandibular joint and muscle anatomy. In adults, jaw muscles also differ markedly from limb and trunk muscles in composition and distribution of fibre types. It can be assumed that age-related changes between young age to adulthood in terms of craniofacial growth, teeth eruption, and improvement of jaw functions are paralleled by alterations also in composition and distribution of jaw muscle fibre types. To address this question, we have examined the fibre type composition of the human masseter, a jaw closing muscle, at young age. For comparison, the young biceps brachii was examined. The results were compared with previous data for adult masseter and biceps muscles. Young masseter and biceps were similar in that type I fibres outnumbered other fibre types and were of the same diameter. However, they differed in composition of other fibre types. Young masseter contained fibre types I, IM, IIC, IIAB, IIB, and scarce IIA, with regional differences, whereas young biceps showed types I, IIA, IIAB, and few IIB. Young masseter differed from young biceps also by smaller type II fibre diameter and by containing fetal MyHC. In addition, the masseter and biceps differed in age-related changes of composition and distribution of fibre types between young age and adulthood. We conclude that the human masseter is specialized in fibre types already at young age and shows a unique fibre type growth pattern, in concordance with being a separate allotype of muscle.

Ort, förlag, år, upplaga, sidor
Wiley-Liss, Inc., 2011
Nyckelord
enzyme- and immuno-histochemistry, fibre types, jaw, limb, morphology, myosin heavy chain
Nationell ämneskategori
Odontologi
Forskningsämne
anatomi
Identifikatorer
urn:nbn:se:umu:diva-46418 (URN)10.1002/ar.21272 (DOI)
Tillgänglig från: 2011-09-01 Skapad: 2011-09-01 Senast uppdaterad: 2018-06-08Bibliografiskt granskad
Österlund, C., Liu, J.-X., Thornell, L.-E. & Eriksson, P.-O. (2011). Muscle spindle composition and distribution in human young masseter and biceps brachii muscles reveal early growth and maturation. Anatomical Record, 294(4), 683-693
Öppna denna publikation i ny flik eller fönster >>Muscle spindle composition and distribution in human young masseter and biceps brachii muscles reveal early growth and maturation
2011 (Engelska)Ingår i: Anatomical Record, ISSN 0003-276X, E-ISSN 1097-0185, Vol. 294, nr 4, s. 683-693Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Significant changes in extrafusal fiber type composition take place in the human masseter muscle from young age, 3-7 years, to adulthood, in parallel with jaw-face skeleton growth, changes of dentitions and improvement of jaw functions. As motor and sensory control systems of muscles are interlinked, also the intrafusal fiber population, that is, muscle spindles, should undergo age-related changes in fiber type appearance. To test this hypothesis, we examined muscle spindles in the young masseter muscle and compared the result with previous data on adult masseter spindles. Also muscle spindles in the young biceps brachii muscle were examined. The result showed that muscle spindle composition and distribution were alike in young and adult masseter. As for the adult masseter, young masseter contained exceptionally large muscle spindles, and with the highest spindle density and most complex spindles found in the deep masseter portion. Hence, contrary to our hypothesis, masseter spindles do not undergo major morphological changes between young age and adulthood. Also in the biceps, young spindles were alike adult spindles. Taken together, the results showed that human masseter and biceps muscle spindles are morphologically mature already at young age. We conclude that muscle spindles in the human young masseter and biceps precede the extrafusal fiber population in growth and maturation. This in turn suggests early reflex control and proprioceptive demands in learning and maturation of jaw motor skills. Similarly, well-developed muscle spindles in young biceps reflect early need of reflex control in learning and performing arm motor behavior.

Ort, förlag, år, upplaga, sidor
Wiley-Liss, Inc., 2011
Nyckelord
fiber types; intrafusal fibers; jaw muscle; skeletal muscle
Nationell ämneskategori
Odontologi
Forskningsämne
odontologi
Identifikatorer
urn:nbn:se:umu:diva-41345 (URN)10.1002/ar.21347 (DOI)21370492 (PubMedID)
Tillgänglig från: 2011-03-23 Skapad: 2011-03-23 Senast uppdaterad: 2018-06-08Bibliografiskt granskad
Thornell, L.-E. (2011). Sarcopenic obesity: satellite cells in the aging muscle. Current opinion in clinical nutrition and metabolic care, 14(1), 22-27
Öppna denna publikation i ny flik eller fönster >>Sarcopenic obesity: satellite cells in the aging muscle
2011 (Engelska)Ingår i: Current opinion in clinical nutrition and metabolic care, ISSN 1363-1950, E-ISSN 1473-6519, Vol. 14, nr 1, s. 22-27Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Human satellite cells are indispensable for maintenance of human muscle mass, but their implications in the pathogenesis of sarcopenia and sarcopenic obesity are still under debate. Nevertheless, satellite cell activation upon exercise seems unequivocally together with adequate nutrition to be the most effective countermeasure for sarcopenia and sarcopenic obesity.

Nyckelord
adipogenesis; muscle damage; regeneration; satellite cells; stem cells
Nationell ämneskategori
Näringslära
Identifikatorer
urn:nbn:se:umu:diva-41362 (URN)10.1097/MCO.0b013e3283412260 (DOI)21088571 (PubMedID)
Tillgänglig från: 2011-03-23 Skapad: 2011-03-23 Senast uppdaterad: 2018-06-08Bibliografiskt granskad
Nordin, A., Larsson, E., Thornell, L.-E. & Holmberg, M. (2011). Tissue-specific splicing of ISCU results in a skeletal muscle phenotype in myopathy with lactic acidosis, while complete loss of ISCU results in early embryonic death in mice. Human Genetics, 129(4), 371-378
Öppna denna publikation i ny flik eller fönster >>Tissue-specific splicing of ISCU results in a skeletal muscle phenotype in myopathy with lactic acidosis, while complete loss of ISCU results in early embryonic death in mice
2011 (Engelska)Ingår i: Human Genetics, ISSN 0340-6717, E-ISSN 1432-1203, Vol. 129, nr 4, s. 371-378Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Hereditary myopathy with lactic acidosis (HML) is caused by an intron mutation in the iron-sulphur cluster assembly gene (ISCU) leading to incorporation of intron sequence into the mRNA. This results in a deficiency of Fe-S cluster proteins, affecting the TCA cycle and the respiratory chain. The proteins involved in the Fe-S machinery are evolutionary conserved and shown to be fundamental in all organisms examined. ISCU is expressed at high levels in numerous tissues in mammals, including high metabolic tissues like the heart, suggesting that a drastic mutation in the ISCU gene would be damaging to all energy-demanding organs. In spite of this, the symptoms in patients with HML are restricted to skeletal muscle, and it has been proposed that splicing events may contribute to the muscle specificity. In this study we confirm that a striking difference in the splicing pattern of mutant ISCU exists between different tissues. The highest level of incorrectly spliced ISCU mRNA was found in skeletal muscle, while the normal splice form predominated in patient heart. The splicing differences were also reflected at a functional level, where loss of Fe-S cluster carrying enzymes and accumulation of iron were present in muscle, but absent in other tissues. We also show that complete loss of ISCU in mice results in early embryonic death. The mice data confirm a fundamental role for ISCU in mammals and further support tissue-specific splicing as the major mechanism limiting the phenotype to skeletal muscle in HML.

Nyckelord
iron-sulfur proteins; succinate-dehydrogenase; paroxysmal myoglobinuria; deficiency; exercise; clusters; mutation; mitochondria; metabolism; maturation
Nationell ämneskategori
Medicinsk genetik
Forskningsämne
medicin
Identifikatorer
urn:nbn:se:umu:diva-40798 (URN)10.1007/s00439-010-0931-3 (DOI)000289275200002 ()21165651 (PubMedID)
Tillgänglig från: 2011-03-09 Skapad: 2011-03-09 Senast uppdaterad: 2018-06-08Bibliografiskt granskad
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