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Derba-Maceluch, Marta
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Ratke, C., Terebieniec, B. K., Winestrand, S., Derba-Maceluch, M., Grahn, T., Schiffthaler, B., . . . Mellerowicz, E. J. (2018). Downregulating aspen xylan biosynthetic GT43 genes in developing wood stimulates growth via reprograming of the transcriptome. New Phytologist, 219(1), 230-245
Öppna denna publikation i ny flik eller fönster >>Downregulating aspen xylan biosynthetic GT43 genes in developing wood stimulates growth via reprograming of the transcriptome
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2018 (Engelska)Ingår i: New Phytologist, ISSN 0028-646X, Vol. 219, nr 1, s. 230-245Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Xylan is one of the main compounds determining wood properties in hardwood species. The xylan backbone is thought to be synthesized by a synthase complex comprising two members of the GT43 family. We downregulated all GT43 genes in hybrid aspen (Populus tremulaxtremuloides) to understand their involvement in xylan biosynthesis.

All three clades of the GT43 family were targeted for downregulation using RNA interference individually or in different combinations, either constitutively or specifically in developing wood.

Simultaneous downregulation in developing wood of the B (IRX9) and C (IRX14) clades resulted in reduced xylan Xyl content relative to reducing end sequence, supporting their role in xylan backbone biosynthesis. This was accompanied by a higher lignocellulose saccharification efficiency. Unexpectedly, GT43 suppression in developing wood led to an overall growth stimulation, xylem cell wall thinning and a shift in cellulose orientation. Transcriptome profiling of these transgenic lines indicated that cell cycling was stimulated and secondary wall biosynthesis was repressed. We suggest that the reduced xylan elongation is sensed by the cell wall integrity surveying mechanism in developing wood.

Our results show that wood-specific suppression of xylan-biosynthetic GT43 genes activates signaling responses, leading to increased growth and improved lignocellulose saccharification.

Nyckelord
cellulose microfibril angle, GT43, Populus, saccharification, secondary wall, wood development, xylan biosynthesis
Nationell ämneskategori
Växtbioteknologi
Identifikatorer
urn:nbn:se:umu:diva-150384 (URN)10.1111/nph.15160 (DOI)000434153200026 ()29708593 (PubMedID)2-s2.0-85046148362 (Scopus ID)
Projekt
Bio4Energy
Tillgänglig från: 2018-08-06 Skapad: 2018-08-06 Senast uppdaterad: 2020-01-30Bibliografiskt granskad
Wang, Z., Pawar, P., Derba-Maceluch, M., Hedenström, M., Chong, S.-L., Tenkanen, M., . . . Jönsson, L. J.Bioprocessing properties of hybrid aspen expressing a Carbohydrate Esterase Family 5 acetyl xylan esterase under control of a wood-specific promoter.
Öppna denna publikation i ny flik eller fönster >>Bioprocessing properties of hybrid aspen expressing a Carbohydrate Esterase Family 5 acetyl xylan esterase under control of a wood-specific promoter
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(Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
Nationell ämneskategori
Pappers-, massa- och fiberteknik
Identifikatorer
urn:nbn:se:umu:diva-145113 (URN)
Tillgänglig från: 2018-02-21 Skapad: 2018-02-21 Senast uppdaterad: 2020-01-30Bibliografiskt granskad
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