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Strömberg, Jessica
Publications (6 of 6) Show all publications
Strömberg, J., Lundgren, P., Taube, M., Bäckström, T., Wang, M. & Haage, D. (2009). The effect of the neuroactive steroid 5β-pregnane-3β, 20(R)-diol on the time course of GABA evoked currents is different to that of pregnenolone sulphate. European Journal of Pharmacology, 605(1-3), 78-86
Open this publication in new window or tab >>The effect of the neuroactive steroid 5β-pregnane-3β, 20(R)-diol on the time course of GABA evoked currents is different to that of pregnenolone sulphate
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2009 (English)In: European Journal of Pharmacology, ISSN 0014-2999, E-ISSN 1879-0712, Vol. 605, no 1-3, p. 78-86Article in journal (Refereed) Published
Abstract [en]

The endogenous progesterone metabolite allopregnanolone has a number of properties including anesthetic, sedative, antiepileptic, anxiolytic, impaired memory function and negative mood symptoms. Allopregnanolone is a potent positive GABA(A) receptor function modulators. In contrast, 3beta-hydroxy-steroids (3beta-steroids) usually modulate the GABA(A) receptor negatively. They have attracted some interest for their possible use as therapeutic agents that could counteract the negative symptoms induced by allopregnanolone. Two hypotheses for the action of 3beta-steroids have been proposed: 1) 3beta-steroids act in a similar way to pregnenolone sulphate, which non-competitively reduces GABA(A) receptor activity. 2) 3beta-steroids specifically antagonize the effect of allopregnanolone. We have therefore tried to clarify this issue by comparing the effect of pregnenolone sulphate and 5beta-pregnane-3beta, 20(R)-diol on the GABA-evoked currents by the patch clamp technique on neurons from the medial preoptic nucleus. Both pregnenolone sulphate and 5beta-pregnane-3beta, 20(R)-diol increase the desensitization rate of the current response evoked by a 2 s GABA application. However, their effects on other parameters of the GABA evoked currents differed in degree and sometimes even in direction. The actions of pregnenolone sulphate and 5beta-pregnane-3beta, 20(R)-diol were not altered in the presence of allopregnanolone, which indicates that they do not directly interact with allopregnanolone. In addition, when 5beta-pregnane-3beta, 20(R)-diol was tested on spontaneous inhibitory postsynaptic currents (sIPSCs), it dramatically reduced the allopregnanolone-induced prolongation of the decay time constant but it had no effect on the decay under control conditions. In conclusion, the effect of 5beta-pregnane-3beta, 20(R)-diol on GABA-evoked currents is different to that of pregnenolone sulphate in medial preoptic nucleus neurons.

Keyword
Patch clamp; sIPSCs; Tau decay; Desensitization; (Rat)
National Category
Obstetrics, Gynecology and Reproductive Medicine
Research subject
Obstetrics and Gynaecology
Identifiers
urn:nbn:se:umu:diva-36809 (URN)10.1016/j.ejphar.2008.12.038 (DOI)19168059 (PubMedID)
Available from: 2010-10-12 Created: 2010-10-12 Last updated: 2017-12-12Bibliographically approved
Strömberg, J., Haage, D., Taube, M., Bäckström, T. & Lundgren, P. (2006). Neurosteroid modulation of allopregnanolone and GABA effect on the GABA-A receptor. Neuroscience, 143(1), 73-81
Open this publication in new window or tab >>Neurosteroid modulation of allopregnanolone and GABA effect on the GABA-A receptor
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2006 (English)In: Neuroscience, ISSN 0306-4522, E-ISSN 1873-7544, Vol. 143, no 1, p. 73-81Article in journal (Refereed) Published
Keyword
Analysis of Variance, Animals, Brain/cytology, Cells; Cultured, Chloride Channels/drug effects/physiology, Chlorides/metabolism, Dose-Response Relationship; Drug, Drug Interactions, Male, Membrane Potentials/drug effects/physiology/radiation effects, Neurons/*drug effects/physiology, Patch-Clamp Techniques/methods, Pregnanolone/*pharmacology, Rats, Rats; Wistar, Receptors; GABA-A/*metabolism, Steroids/*pharmacology, gamma-Aminobutyric Acid/*pharmacology
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:umu:diva-10000 (URN)10.1016/j.neuroscience.2006.07.031 (DOI)16938407 (PubMedID)
Available from: 2008-06-04 Created: 2008-06-04 Last updated: 2017-12-14Bibliographically approved
Strömberg, J., Bäckström, T. & Lundgren, P. (2005). Rapid non-genomic effect of glucocorticoid metabolites and neurosteroids on the gamma-aminobutyric acid-A receptor. European Journal of Neuroscience, 21(8), 2083-2088
Open this publication in new window or tab >>Rapid non-genomic effect of glucocorticoid metabolites and neurosteroids on the gamma-aminobutyric acid-A receptor
2005 (English)In: European Journal of Neuroscience, ISSN 0953-816X, E-ISSN 1460-9568, Vol. 21, no 8, p. 2083-2088Article in journal (Other academic) Published
Keyword
Animals, Cerebral Cortex/*cytology, Chlorides/metabolism, Desoxycorticosterone/*analogs & derivatives/pharmacology, Dose-Response Relationship; Drug, Drug Interactions, Glucocorticoids/*metabolism/*pharmacology, Male, Neurons/*cytology/drug effects/metabolism, Pregnanolone/pharmacology, Rats, Rats; Wistar, Receptors; GABA-A/*metabolism, Synaptosomes/*drug effects, gamma-Aminobutyric Acid/pharmacology
Identifiers
urn:nbn:se:umu:diva-9999 (URN)10.1111/j.1460-9568.2005.04047.x (DOI)15869504 (PubMedID)
Available from: 2008-06-04 Created: 2008-06-04 Last updated: 2017-12-14Bibliographically approved
Lundgren, P., Strömberg, J., Bäckström, T. & Wang, M. (2003). Allopregnanolone-stimulated GABA-mediated chloride ion flux is inhibited by 3beta-hydroxy-5alpha-pregnane-20-one (isoallopregnanolone). Brain Research, 982(1), 45-53
Open this publication in new window or tab >>Allopregnanolone-stimulated GABA-mediated chloride ion flux is inhibited by 3beta-hydroxy-5alpha-pregnane-20-one (isoallopregnanolone)
2003 (English)In: Brain Research, ISSN 0006-8993, E-ISSN 1872-6240, Vol. 982, no 1, p. 45-53Article in journal (Refereed) Published
Identifiers
urn:nbn:se:umu:diva-2183 (URN)10.1016/S0006-8993(03)02939-1 (DOI)12915239 (PubMedID)
Available from: 2007-03-27 Created: 2007-03-27 Last updated: 2017-12-14Bibliographically approved
Bäckström, T., Andersson, A., Andreén, L., Birzniece, V., Bixo, M., Björn, I., . . . Zingmark, E. (2003). Pathogenesis in menstrual cycle-linked CNS disorders.. Annals of the New York Academy of Sciences, 1007, 42-53
Open this publication in new window or tab >>Pathogenesis in menstrual cycle-linked CNS disorders.
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2003 (English)In: Annals of the New York Academy of Sciences, ISSN 0077-8923, E-ISSN 1749-6632, Vol. 1007, p. 42-53Article, review/survey (Other academic) Published
Keyword
Affect/physiology, Animals, Female, Humans, Menstrual Cycle/*physiology, Mood Disorders/*etiology/physiopathology/psychology, Pregnanolone/physiology, Premenstrual Syndrome/*etiology/physiopathology/psychology, Receptors; GABA-A/physiology
Identifiers
urn:nbn:se:umu:diva-16450 (URN)10.1196/annals.1286.005 (DOI)14993039 (PubMedID)
Available from: 2007-09-28 Created: 2007-09-28 Last updated: 2017-12-14Bibliographically approved
Strömberg, J., Lundgren, P., Taube, M., Bäckström, T., Wang, M. & Haage, D.The neuroactive steroid 5beta-pregnane-3beta, 20(R)-diol alters the kinetic properties of the GABA-A receptor differently from pregnenolone-sulfate.
Open this publication in new window or tab >>The neuroactive steroid 5beta-pregnane-3beta, 20(R)-diol alters the kinetic properties of the GABA-A receptor differently from pregnenolone-sulfate
Show others...
(English)Manuscript (Other academic)
Identifiers
urn:nbn:se:umu:diva-2185 (URN)
Available from: 2007-03-27 Created: 2007-03-27 Last updated: 2015-11-11Bibliographically approved
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