umu.sePublications
Change search
Link to record
Permanent link

Direct link
BETA
Danielsson, Karin
Publications (10 of 17) Show all publications
Danielsson, K., Nylander, E., Sjöström, M. & Ebrahimi, M. (2018). Epstein-Barr virus is not detected in mucosal lichen planus. Medicina Oral, 23(5), e560-e563, Article ID 22617.
Open this publication in new window or tab >>Epstein-Barr virus is not detected in mucosal lichen planus
2018 (English)In: Medicina Oral, ISSN 1698-4447, E-ISSN 1698-6946, Vol. 23, no 5, p. e560-e563, article id 22617Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Lichen planus (LP) is a chronic inflammatory, immunological, mucocutaneous disease can affect skin, genital and oral mucosa. Oral lichen planus (OLP) is the most common noninfectious, chronic inflammatory oral disease affecting 1-2% of the general adult population. World Health Organization (WHO) classifies OLP as a potentially malignant disorder. Epstein Barr virus or human herpesvirus-4, is a member of the herpes virus family and one of the most ubiquitous viruses known to human, infecting approximately 90% of the world's adult population. The virus often infects B lymphocytes resulting in a wide spectrum of mucocutaneous and systemic diseases, ranging from mild lesions to aggressive malignancies. The aim of this study was to investigate expression of the EBV encoded RNAs EBER1 and EBER2 in oral and genital lichen planus and compare results with normal tissues in situ hybridization which is considered the golden standard for detection of EBER.

MATERIAL AND METHODS: A total of 68 biopsies, 25 oral LP, 26 genital LP, 10 oral controls and finally 7 genital controls were analysed using situ hybridization.

RESULT: All samples had RNA as shown by the control slide, whereas no case contained neither EBER1 nor EBER2.

CONCLUSIONS: Based on results from our study EBV is not involved in aetiology of lichen planus.

Place, publisher, year, edition, pages
Medicina Oral, 2018
Keywords
Mucosal lichen planus, Epstein - Barr virus
National Category
Dentistry
Identifiers
urn:nbn:se:umu:diva-151757 (URN)10.4317/medoral.22617 (DOI)000443304400009 ()30148472 (PubMedID)
Available from: 2018-09-13 Created: 2018-09-13 Last updated: 2018-09-14Bibliographically approved
Sgaramella, N., Wilms, T., Boldrup, L., Loljung, L., Gu, X., Coates, P. J., . . . Nylander, K. (2018). Ethnicity based variation in expression of E-cadherin in patients with squamous cell carcinoma of the oral tongue. Oncology Letters, 16(5), 6603-6607
Open this publication in new window or tab >>Ethnicity based variation in expression of E-cadherin in patients with squamous cell carcinoma of the oral tongue
Show others...
2018 (English)In: Oncology Letters, ISSN 1792-1074, E-ISSN 1792-1082, Vol. 16, no 5, p. 6603-6607Article in journal (Refereed) Published
Abstract [en]

The oral tongue is the most common site for tumours within the oral cavity. Despite intense research, there has been no improvement in the survival rate for patients with oral tongue squamous cell carcinoma (OTSCC) during the last decades. Differences between oral cancer patients based on ethno-geographical distribution have been reported. The present study used immunohistochemistry to evaluate commonly used markers of cancer cell phenotypes, E-cadherin, -catenin and cytokeratins 5 and 19, in 120 patients with OTSCC. To evaluate the impact of ethnicity, patients from Sweden and Italy were included. A higher proportion of Swedish patients exhibited high expression of E-cadherin in their tumours (P=0.039), and high levels of E-cadherin in Swedish OTSCC patients that had succumbed to their disease were associated with poor prognosis. These data demonstrated differences in the pathological characteristics of OTSCC between two different European populations. The findings emphasise the need to take ethnicity/geographical location of patients into account when comparing results from different studies of OTSCC.

Place, publisher, year, edition, pages
Spandidos Publications, 2018
Keywords
E-cadherin, beta catenin, squamous cell carcinoma, oral tongue, ethnicity
National Category
Cancer and Oncology
Identifiers
urn:nbn:se:umu:diva-153550 (URN)10.3892/ol.2018.9452 (DOI)000448433500141 ()30405799 (PubMedID)
Funder
Swedish Cancer Society, 17 06 63Västerbotten County Council, MEYS-NPSI-LO1413
Available from: 2018-11-22 Created: 2018-11-22 Last updated: 2019-05-10Bibliographically approved
Danielsson, K., Olah, J., Zohori-Zangeneh, R., Nylander, E. & Ebrahimi, M. (2018). Increased expression of p16 in both oral and genital lichen planus. Medicina Oral, 23(4), e449-e453
Open this publication in new window or tab >>Increased expression of p16 in both oral and genital lichen planus
Show others...
2018 (English)In: Medicina Oral, ISSN 1698-4447, E-ISSN 1698-6946, Vol. 23, no 4, p. e449-e453Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Lichen Planus, LP, is an inflammatory disease of possible autoimmune origin affecting mainly oral and genital mucosa and skin. According to the WHO oral LP is considered a potentially malignant disorders. The p16 tumour suppressor protein can act as an inhibitor of cyclin dependent kinases 4 and 6 and thus down regulate cell cycle progression. Since the discovery of p16 several studies have evaluated its expression in various forms of human cancers. The aim of this study was to evaluate and compare the expression of p16 in oral and genital LP and corresponding healthy mucosa.

MATERIAL AND METHODS: A total of 76 cases of oral LP (OLP), 34 cases of genital LP (GLP), 12 cases of healthy oral and 9 cases of healthy genital mucosa were analysed by the use of immunohistochemistry.

RESULTS: Data showed p16 to be highly expressed in both oral and genital LP, higher than in oral (p=0.000), and genital controls (p=0.002).

CONCLUSIONS: Results suggest that the over-expression of p16 seen in LP play a part in the histopathology of the disease.

Keywords
p16, inflammation, oral, genital, lichen planus, malignant risk
National Category
Dentistry
Identifiers
urn:nbn:se:umu:diva-150603 (URN)10.4317/medoral.22432 (DOI)000437438600011 ()29924765 (PubMedID)
Available from: 2018-08-14 Created: 2018-08-14 Last updated: 2018-08-21Bibliographically approved
Sgaramella, N., Gu, X., Boldrup, L., Coates, P. J., Fåhraeus, R., Califano, L., . . . Nylander, K. (2018). Searching for new targets and treatments in the battle against squamous cell carcinoma of the head and neck, with specific focus on tumours of the tongue. Current Topics in Medicinal Chemistry, 18(3), 214-218
Open this publication in new window or tab >>Searching for new targets and treatments in the battle against squamous cell carcinoma of the head and neck, with specific focus on tumours of the tongue
Show others...
2018 (English)In: Current Topics in Medicinal Chemistry, ISSN 1568-0266, E-ISSN 1873-4294, Vol. 18, no 3, p. 214-218Article, review/survey (Refereed) Published
Abstract [en]

Squamous cell carcinoma of the head and neck, SCCHN, is a heterogeneous group of tumours not only concerning the site of origin but also regarding aetiology. The 5-year survival for the whole group of SCCHN tumours has not significantly improved over the last 20-25 years. Apart from tumour spread to lymph nodes, N status, gains and losses of specific chromosomes are the only factors shown to be independent prognostic markers for these tumours. Worldwide, an increasing number of people ≤ 40 years are seen being affected by tongue SCC, the most common tumour within the SCCHN group. Even without any clinical signs of metastasis, up to 30% of all tongue SCC have histologically detectable spread to lymph nodes. In this mini review, field cancerization, tumour microenvironment, the so called EMT (epithelial mesenchymal transition) process and the role of viruses in development of SCCHN are discussed as well as potential new therapeutic targets. For the group of tongue SCC, with the increasing incidence seen in young patients and particularly women, new data with impact on prognosis and treatment are urgently needed. But as long as data from the analyses of several sub sites are presented as valid for the whole group of tumours, this vital point is missed.

Place, publisher, year, edition, pages
Bentham Science Publishers, 2018
Keywords
squamous cell carcinoma, tongue, prognosis, therapy, miRNA, HPV, EBV, p63
National Category
Cancer and Oncology
Identifiers
urn:nbn:se:umu:diva-148841 (URN)10.2174/1568026618666180116121624 (DOI)000432250800006 ()29345578 (PubMedID)
Available from: 2018-06-12 Created: 2018-06-12 Last updated: 2019-05-10Bibliographically approved
Danielsson, K., Ebrahimi, M., Nylander, E., Wahlin, Y. B. & Nylander, K. (2017). Alterations in factors involved in differentiation and barrier function in the epithelium in oral and genital lichen planus. Acta Dermato-Venereologica, 97(2), 214-218
Open this publication in new window or tab >>Alterations in factors involved in differentiation and barrier function in the epithelium in oral and genital lichen planus
Show others...
2017 (English)In: Acta Dermato-Venereologica, ISSN 0001-5555, E-ISSN 1651-2057, Vol. 97, no 2, p. 214-218Article in journal, Editorial material (Refereed) Published
Abstract [en]

Lichen planus is a chronic recurrent inflammatory disease affecting both skin and mucosa, mainly in oral and/or genital regions. Keratinocytes go through a well-regulated process of proliferation and differentiation, alterations in which may result in defects in the protective epithelial barrier. Long-term barrier impairment might lead to chronic inflammation. In order to broaden our understanding of the differentiation process in mucosal lichen planus, we mapped the expression of 4 factors known to be involved in differentiation. Biopsies were collected from oral and genital lichen planus lesions and normal controls. Altered expression of all 4 factors in epithelium from lichen planus lesions was found, clearly indicating disturbed epithelial differentiation in lichen planus lesions.

Keywords
lichen planus, differentiation, epithelial barrier
National Category
Dentistry Dermatology and Venereal Diseases
Research subject
Medical Cell Biology; Pathology
Identifiers
urn:nbn:se:umu:diva-125780 (URN)10.2340/00015555-2533 (DOI)000393895500009 ()27599552 (PubMedID)
Available from: 2016-09-16 Created: 2016-09-16 Last updated: 2019-05-10Bibliographically approved
Strömberg, N., Esberg, A., Sheng, N., Mårell, L., Löfgren-Burström, A., Danielsson, K. & Källestål, C. (2017). Genetic- and Lifestyle-dependent Dental Caries Defined by the Acidic Proline-rich Protein Genes PRH1 and PRH2. EBioMedicine, 26, 38-46
Open this publication in new window or tab >>Genetic- and Lifestyle-dependent Dental Caries Defined by the Acidic Proline-rich Protein Genes PRH1 and PRH2
Show others...
2017 (English)In: EBioMedicine, ISSN 0360-0637, E-ISSN 2352-3964, Vol. 26, p. 38-46Article in journal (Refereed) Published
Abstract [en]

Dental caries is a chronic infectious disease that affects billions of people with large individual differences in activity. We investigated whether PRH1 and PRH2 polymorphisms in saliva acidic proline-rich protein (PRP) receptors for indigenous bacteria match and predict individual differences in the development of caries. PRH1 and PRH2 variation and adhesion of indigenous and cariogenic (Streptococcus mutans) model bacteria were measured in 452 12-year-old Swedish children along with traditional risk factors and related to caries at baseline and after 5-years. The children grouped into low-to-moderate and high susceptibility phenotypes for caries based on allelic PRH1, PRH2 variation. The low-to-moderate susceptibility children (P1 and P4a-) experienced caries from eating sugar or bad oral hygiene or infection by S. mutans. The high susceptibility P4a (Db, PIF, PRP12) children had more caries despite receiving extra prevention and irrespective of eating sugar or bad oral hygiene or S. mutans-infection. They instead developed 3.9-fold more caries than P1 children from plaque accumulation in general when treated with orthodontic multibrackets; and had basic PRP polymorphisms and low DMBT1-mediated S. mutans adhesion as additional susceptibility traits. The present findings thus suggest genetic autoimmune-like (P4a) and traditional life style (P1) caries, providing a rationale for individualized oral care.

Keywords
Acidic proline-rich proteins, Chronic infections, Dental caries, Host susceptibility, PRH1, PRH2
National Category
Dentistry
Identifiers
urn:nbn:se:umu:diva-143131 (URN)10.1016/j.ebiom.2017.11.019 (DOI)29191562 (PubMedID)
Available from: 2017-12-18 Created: 2017-12-18 Last updated: 2018-06-09Bibliographically approved
Sgaramella, N., Lindell Jonsson, E., Boldrup, L., Califano, L., Coates, P. J., Tartaro, G., . . . Nylander, K. (2016). High expression of podoplanin in squamous cell carcinoma of the tongue occurs predominantly in patients ≤ 40 years but does not correlate with tumour spread. The Journal of Pathology: Clinical Research, 2(1), 3-8
Open this publication in new window or tab >>High expression of podoplanin in squamous cell carcinoma of the tongue occurs predominantly in patients ≤ 40 years but does not correlate with tumour spread
Show others...
2016 (English)In: The Journal of Pathology: Clinical Research, ISSN 2056-4538, Vol. 2, no 1, p. 3-8Article in journal (Refereed) Published
Abstract [en]

More than 30% of patients with squamous cell carcinoma (SCC) of the mobile tongue have clinically undetectable lymph node metastasis. Tumour cells can spread as single cells or collectively. A protein known to play a role in both processes is podoplanin, which is expressed in endothelial cells not only in lymph vessels but also in some aggressive tumours with high invasive and metastatic potential. Here we studied samples from 129 patients with primary SCC of the tongue for expression of podoplanin using immunohistochemistry. mRNA levels were analysed in another 27 cases of tongue SCC with adjacent clinically tumour-free tongue tissue and 14 tongue samples from healthy donors. Higher levels of podoplanin were seen in tumours compared to both normal tongue and clinically normal tongue in the tumour vicinity. No association was found between levels of podoplanin, presence of lymph node metastases or other clinical factors. Patients aged 40 or less were more likely to express high levels of podoplanin protein compared to older patients (p 50.027). We conclude that levels of podoplanin in primary tongue SCCs are not associated with lymph node metastases. However, tongue SCCs arising in young patients (40 years of age) are more likely to express high levels of podoplanin than tongue SCCs that arise in the more elderly. The data suggest that podoplanin has a distinctive role in young patients, who are known to have a poor prognosis: these patients may, therefore, benefit from podoplanin inhibitory therapies.

Place, publisher, year, edition, pages
John Wiley & Sons, 2016
Keywords
squamous cell carcinoma, tongue, podoplanin
National Category
Cancer and Oncology Otorhinolaryngology
Identifiers
urn:nbn:se:umu:diva-121097 (URN)10.1002/cjp2.28 (DOI)000410840100001 ()27499910 (PubMedID)
Available from: 2016-05-26 Created: 2016-05-26 Last updated: 2018-06-07Bibliographically approved
Lundegard, M., Nylander, K. & Danielsson, K. (2015). Difficulties detecting miRNA-203 in human whole saliva by the use of PCR. Medicina Oral, 20(2), E130-E134, Article ID 20172.
Open this publication in new window or tab >>Difficulties detecting miRNA-203 in human whole saliva by the use of PCR
2015 (English)In: Medicina Oral, ISSN 1698-4447, E-ISSN 1698-6946, Vol. 20, no 2, p. E130-E134, article id 20172Article in journal (Refereed) Published
Abstract [en]

Objectives: Oral Lichen Planus (OLP) is a chronic disease of the oral mucosa, and according to the WHO also a pre malignant condition. Micro-RNAs are short non coding RNAs capable of regulating mRNA expression. MiRNA:scan be detected in tissue, blood and human whole saliva (HWS) and recently we have shown miR-203 to be up-regulated in tissue from OLP lesions. Study Design: In order to see whether mRNA as well as miR-203 could be detected also in HWS, saliva from healthy controls and patients with OLP were analysed using two different PCR methods. Results: Results showed low mRNA and miRNA levels in general in HWS samples, making it hard to generate conclusive results. Conclusions: In order to make HWS a valuable source for different analyses, more sensitive PCR techniques capable of detecting very low levels of mRNA and miRNA as well as more efficient methods for extraction of RNA are needed.

Keywords
miRNA-203, saliva, PCR
National Category
Dentistry
Identifiers
urn:nbn:se:umu:diva-102380 (URN)10.4317/medoral.20172 (DOI)000351923500002 ()25475777 (PubMedID)
Available from: 2015-05-07 Created: 2015-04-23 Last updated: 2018-06-07Bibliographically approved
Sgaramella, N., Coates, P. J., Strindlund, K., Loljung, L., Colella, G., Laurell, G., . . . Nylander, K. (2015). Expression of p16 in squamous cell carcinoma of the mobile tongue is independent of HPV infection despite presence of the HPV-receptor syndecan-1. British Journal of Cancer, 113(2), 321-326
Open this publication in new window or tab >>Expression of p16 in squamous cell carcinoma of the mobile tongue is independent of HPV infection despite presence of the HPV-receptor syndecan-1
Show others...
2015 (English)In: British Journal of Cancer, ISSN 0007-0920, E-ISSN 1532-1827, Vol. 113, no 2, p. 321-326Article in journal (Refereed) Published
Abstract [en]

Background: Tongue squamous cell carcinoma (TSCC) is increasing in incidence, especially among young patients and preferably females. Infection with human papilloma virus (HPV) has been suggested as a cause of SCC in the head and neck, and the proportion of oropharyngeal cancers caused by HPV has steadily increased. Methods: Samples from 109 patients with primary TSCC were analysed for the presence of HPV16 by in situ hybridisation and for expression of its surrogate marker p16 and the HPV receptor syndecan-1 by immunhistochemistry. Results: No evidence of HPV16 DNA was observed in the tumours, although one-third showed p16 staining. There was no difference in the expression of the primary HPV receptor, syndecan-1, between TSCC and a group of tonsil SCC. Conclusion: Whereas p16 is expressed in some TSCCs, HPV16 is undetectable, therefore, p16 cannot be used as a surrogate marker for high-risk HPV-infection in this tumour. Despite presence of the HPV-receptor syndecan-1 in TSCC, HPV prefers the tonsillar environment. Lack of p16 associates with worse prognosis primarily in patients aged <= 40 years with tongue SCC. The improved prognosis seen in p16-positive TSCC can be due to induction of a senescent phenotype or an inherent radiosensitivity due to the ability of p16 to inhibit homologous recombination repair.

Place, publisher, year, edition, pages
Nature Publishing Group, 2015
Keywords
squamous cell carcinoma, tongue, HPV, p16, syndecan-1, TRE S, 1995, JOURNAL OF CLINICAL PATHOLOGY, V48, P876 galdi M., 2012, JOURNAL OF ORAL PATHOLOGY & MEDICINE, V41, P16 aturvedi Anil K., 2011, JOURNAL OF CLINICAL ONCOLOGY, V29, P4294
National Category
Cancer and Oncology
Identifiers
urn:nbn:se:umu:diva-106780 (URN)10.1038/bjc.2015.207 (DOI)000357947800017 ()26057450 (PubMedID)
Available from: 2015-08-21 Created: 2015-08-07 Last updated: 2018-06-07Bibliographically approved
Danielsson, K., Coates, P. J., Ebrahimi, M., Nylander, E., Wahlin, Y.-B. & Nylander, K. (2014). Genes Involved in Epithelial Differentiation and Development are Differentially Expressed in Oral and Genital Lichen Planus Epithelium Compared to Normal Epithelium. Acta Dermato-Venereologica, 94(5), 526-530
Open this publication in new window or tab >>Genes Involved in Epithelial Differentiation and Development are Differentially Expressed in Oral and Genital Lichen Planus Epithelium Compared to Normal Epithelium
Show others...
2014 (English)In: Acta Dermato-Venereologica, ISSN 0001-5555, E-ISSN 1651-2057, Vol. 94, no 5, p. 526-530Article in journal (Refereed) Published
Abstract [en]

Lichen planus (LP) is a chronic mucocutaneous disease with unknown cause. Patients with LP often have both oral and genital lesions, but these conditions are often considered as separate diseases and treated accordingly. To find out which genes are differently expressed in mucosal LP compared to normal mucosa and establish whether oral and genital LP are in fact the same disease, whole genome expression analysis was performed on epithelium from 13 patients diagnosed with oral and/or genital LP and normal controls. For confirmation of keratin 4 and corneodesmosin expression, quantitative reverse-transcription PCR and immunohistochemistry were used. Many genes involved in epithelial development and differentiation are differently expressed in epithelium from LP compared to normal epithelium. Several of the differentially expressed genes are common for oral and genital LP and the same biological processes are altered which supports the fact that oral and genital LP are manifestations of the same disease. The change in gene expression indicates that differentiation is altered leading to changes in the epithelial barrier.

Keywords
oral lichen planus, genital lichen planus, epithelial differentiation, corneodesmosin, keratin 4
National Category
Cell and Molecular Biology Medical Genetics
Identifiers
urn:nbn:se:umu:diva-87232 (URN)10.2340/00015555-1803 (DOI)000341676400006 ()24626344 (PubMedID)
Available from: 2014-03-25 Created: 2014-03-25 Last updated: 2018-06-08Bibliographically approved
Organisations

Search in DiVA

Show all publications