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Wahlin, Ylva Britt
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Publications (10 of 25) Show all publications
Danielsson, K., Ebrahimi, M., Nylander, E., Wahlin, Y. B. & Nylander, K. (2017). Alterations in factors involved in differentiation and barrier function in the epithelium in oral and genital lichen planus. Acta Dermato-Venereologica, 97(2), 214-218
Open this publication in new window or tab >>Alterations in factors involved in differentiation and barrier function in the epithelium in oral and genital lichen planus
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2017 (English)In: Acta Dermato-Venereologica, ISSN 0001-5555, E-ISSN 1651-2057, Vol. 97, no 2, p. 214-218Article in journal, Editorial material (Refereed) Published
Abstract [en]

Lichen planus is a chronic recurrent inflammatory disease affecting both skin and mucosa, mainly in oral and/or genital regions. Keratinocytes go through a well-regulated process of proliferation and differentiation, alterations in which may result in defects in the protective epithelial barrier. Long-term barrier impairment might lead to chronic inflammation. In order to broaden our understanding of the differentiation process in mucosal lichen planus, we mapped the expression of 4 factors known to be involved in differentiation. Biopsies were collected from oral and genital lichen planus lesions and normal controls. Altered expression of all 4 factors in epithelium from lichen planus lesions was found, clearly indicating disturbed epithelial differentiation in lichen planus lesions.

Keywords
lichen planus, differentiation, epithelial barrier
National Category
Dentistry Dermatology and Venereal Diseases
Research subject
Medical Cell Biology; Pathology
Identifiers
urn:nbn:se:umu:diva-125780 (URN)10.2340/00015555-2533 (DOI)000393895500009 ()27599552 (PubMedID)
Available from: 2016-09-16 Created: 2016-09-16 Last updated: 2019-05-10Bibliographically approved
Danielsson, K., Coates, P. J., Ebrahimi, M., Nylander, E., Wahlin, Y.-B. & Nylander, K. (2014). Genes Involved in Epithelial Differentiation and Development are Differentially Expressed in Oral and Genital Lichen Planus Epithelium Compared to Normal Epithelium. Acta Dermato-Venereologica, 94(5), 526-530
Open this publication in new window or tab >>Genes Involved in Epithelial Differentiation and Development are Differentially Expressed in Oral and Genital Lichen Planus Epithelium Compared to Normal Epithelium
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2014 (English)In: Acta Dermato-Venereologica, ISSN 0001-5555, E-ISSN 1651-2057, Vol. 94, no 5, p. 526-530Article in journal (Refereed) Published
Abstract [en]

Lichen planus (LP) is a chronic mucocutaneous disease with unknown cause. Patients with LP often have both oral and genital lesions, but these conditions are often considered as separate diseases and treated accordingly. To find out which genes are differently expressed in mucosal LP compared to normal mucosa and establish whether oral and genital LP are in fact the same disease, whole genome expression analysis was performed on epithelium from 13 patients diagnosed with oral and/or genital LP and normal controls. For confirmation of keratin 4 and corneodesmosin expression, quantitative reverse-transcription PCR and immunohistochemistry were used. Many genes involved in epithelial development and differentiation are differently expressed in epithelium from LP compared to normal epithelium. Several of the differentially expressed genes are common for oral and genital LP and the same biological processes are altered which supports the fact that oral and genital LP are manifestations of the same disease. The change in gene expression indicates that differentiation is altered leading to changes in the epithelial barrier.

Keywords
oral lichen planus, genital lichen planus, epithelial differentiation, corneodesmosin, keratin 4
National Category
Cell and Molecular Biology Medical Genetics
Identifiers
urn:nbn:se:umu:diva-87232 (URN)10.2340/00015555-1803 (DOI)000341676400006 ()24626344 (PubMedID)
Available from: 2014-03-25 Created: 2014-03-25 Last updated: 2018-06-08Bibliographically approved
Danielsson, K., Boldrup, L., Rentoft, M., Coates, P., Ebrahimi, M., Nylander, E., . . . Nylander, K. (2013). Autoantibodies and decreased expression of the transcription factor ELF-3 together with increased chemokine pathways support an autoimmune phenotype and altered differentiation in lichen planus located in oral mucosa. Journal of the European Academy of Dermatology and Venereology, 27(11), 1410-1416
Open this publication in new window or tab >>Autoantibodies and decreased expression of the transcription factor ELF-3 together with increased chemokine pathways support an autoimmune phenotype and altered differentiation in lichen planus located in oral mucosa
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2013 (English)In: Journal of the European Academy of Dermatology and Venereology, ISSN 0926-9959, E-ISSN 1468-3083, Vol. 27, no 11, p. 1410-1416Article in journal (Refereed) Published
Abstract [en]

Background  The pathogenesis of oral lichen planus (OLP), a chronic inflammatory disease, is not fully understood. It is known that OLP has autoimmune features, and it is suggested to be an autoimmune disease. ELF-3 is involved in differentiation of keratinocytes and deregulated in different tumours and inflammatory diseases. CXCR-3 and its ligands CXCL-10 and CXCL-11 are increased in autoimmune diseases and linked to Th-1 immune response. Objectives  To analyse and compare expression of ELF-3, CXCR-3, CXCL-10 and CXCL-11 in OLP lesions and controls in whole and microdissected epithelium. Methods  Tissue biopsies from 20 patients clinically and histologically diagnosed with OLP and 20 healthy controls were studied using whole tissues or microdissected epithelium. By the use of qRT-PCR, mRNA levels of ELF-3, CXCR-3, CXCL-10 and CXCL-11 were studied. Western blot was used for analysis of ELF-3 protein expression. Sera from 19 OLP patients and 20 controls were analysed with ELISA in search for autoantibodies. Results  The upregulation of CXCR-3, CXCL-10 and CXCL-11 found in OLP is similar to previous findings showing an autoimmune phenotype in lichen planus (LP) and lichen sclerosus. Decreased expression of the differentiation-related transcription factor ELF-3 was also seen in OLP lesions, and we further demonstrate presence of circulating autoantibodies against the ELF-3 protein in sera from 3 of 19 (16%) LP patients tested. Conclusions  On the basis of these findings, we confirm that OLP shows features of an autoimmune disease and suggest deregulated differentiation of keratinocytes to be one of the causes of the disease phenotype.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2013
National Category
Basic Medicine Dermatology and Venereal Diseases
Identifiers
urn:nbn:se:umu:diva-55418 (URN)10.1111/jdv.12027 (DOI)000325747200011 ()23134363 (PubMedID)
Available from: 2012-05-15 Created: 2012-05-14 Last updated: 2018-06-08Bibliographically approved
Danielsson, K., Ebrahimi, M., Wahlin, Y.-B., Nylander, K. & Boldrup, L. (2013). Reply to increased levels of COX-2 and oral lichen planus by P.D. Pigatto, F. Spaderi, G.P. Bombeccari, G. Guzzi by Danielsson et al. Journal of the European Academy of Dermatology and Venereology, 27(3), 395-396
Open this publication in new window or tab >>Reply to increased levels of COX-2 and oral lichen planus by P.D. Pigatto, F. Spaderi, G.P. Bombeccari, G. Guzzi by Danielsson et al
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2013 (English)In: Journal of the European Academy of Dermatology and Venereology, ISSN 0926-9959, E-ISSN 1468-3083, Vol. 27, no 3, p. 395-396Article in journal, Editorial material (Refereed) Published
Place, publisher, year, edition, pages
Wiley-Blackwell, 2013
National Category
Dentistry Dermatology and Venereal Diseases
Identifiers
urn:nbn:se:umu:diva-57238 (URN)10.1111/j.1468-3083.2012.04508.x (DOI)000315115900044 ()22429324 (PubMedID)
Available from: 2012-07-10 Created: 2012-07-10 Last updated: 2018-06-08Bibliographically approved
Danielsson, K., Wahlin, Y.-B., Gu, X., Boldrup, L. & Nylander, K. (2012). Altered expression of miR-21, miR-125b, and miR-203 indicates a role for these microRNAs in oral lichen planus. Journal of Oral Pathology & Medicine, 41(1), 90-95
Open this publication in new window or tab >>Altered expression of miR-21, miR-125b, and miR-203 indicates a role for these microRNAs in oral lichen planus
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2012 (English)In: Journal of Oral Pathology & Medicine, ISSN 0904-2512, E-ISSN 1600-0714, Vol. 41, no 1, p. 90-95Article in journal (Refereed) Published
Abstract [en]

Background: Oral lichen planus (OLP), which is a chronic inflammatory disease of the oral mucosa with unknown etiology, affects about 2% of the population. MicroRNAs are small non-coding RNAs involved in normal processes such as development and differentiation as well as progression of human diseases. The aim of this study was to investigate the expression of miR-21, miR-125b, and miR-203 and to compare RNA levels of their potential targets, the tumor suppressor p53 and its relative p63, both known to be deregulated in OLP.

Methods: In biopsies from 20 patients with OLP and 20 age- and sex-matched healthy controls, epithelium was laser dissected and analyzed for the expression of miR-21, miR-125b, miR-203, p53, and p63 using qRT/PCR.

Results: Increased expression of miR-21 and miR-203, decreased expression of miR-125, and down-regulation of p53 and ΔNp63 RNA were seen in OLP compared to normal oral mucosa. When comparing microRNA expression to levels of p53 and p63 RNA, a significant negative correlation was seen between ΔNp63 and miR-203 and between miR-21 and p53, respectively.

Conclusion: Results indicate a role for the studied microRNAs in changes seen in OLP.

Keywords
miR-125b;miR-203;miR-21;oral lichen planus
National Category
Dentistry
Research subject
Odontology; Pathology
Identifiers
urn:nbn:se:umu:diva-49757 (URN)10.1111/j.1600-0714.2011.01084.x (DOI)21943223 (PubMedID)
Available from: 2011-11-17 Created: 2011-11-17 Last updated: 2018-06-08Bibliographically approved
Nylander, E., Ebrahimi, M., Wahlin, Y.-B., Boldrup, L. & Nylander, K. (2012). Changes in miRNA expression in sera and correlation to duration of disease in patients with multifocal mucosal lichen planus.. Journal of Oral Pathology & Medicine, 41(1), 86-89
Open this publication in new window or tab >>Changes in miRNA expression in sera and correlation to duration of disease in patients with multifocal mucosal lichen planus.
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2012 (English)In: Journal of Oral Pathology & Medicine, ISSN 0904-2512, E-ISSN 1600-0714, Vol. 41, no 1, p. 86-89Article in journal (Refereed) Published
Abstract [en]

Background: Mucosal lichen planus is a severe variant of lichen planus, Lichen planus (LP), which in many ways affect patients' lives. The aetiology is not fully understood, and there is no treatment clearing the disease once and for all. Oral LP has by the WHO been classified as a precancerous lesion. Micro-RNAs, miRNAs, are non-coding, small single-stranded RNAs involved in biological processes like apoptosis, proliferation, differentiation, metastasis, angiogenesis and immune response.

Methods and Results: In sera from 30 patients with multifocal mucosal LP, 15 miRNAs were identified as significantly differentially expressed compared with controls. The three most up-regulated miRNAs are all connected to oral squamous cell carcinoma or epithelial carcinoma in general.

Discussion: Even if no specific LP-associated miRNA profile was found, data clearly indicate that miRNAs could play a role in the earlier phases of lichen planus.

Place, publisher, year, edition, pages
Wiley, 2012
Keywords
miRNA, multifocal mucosal lichen planus
National Category
Dentistry
Research subject
Odontology
Identifiers
urn:nbn:se:umu:diva-45639 (URN)10.1111/j.1600-0714.2011.01063.x (DOI)21777290 (PubMedID)
Available from: 2011-08-08 Created: 2011-08-08 Last updated: 2018-06-08Bibliographically approved
Danielsson, K., Wahlin, Y.-B., Boldrup, L. & Nylander, K. (2012). Decreased expression of ELF-3 indicating disturbed differentiation in oral lichen planus. Oral Diseases, 18(Special Issue, Suppl. 1), 20-20
Open this publication in new window or tab >>Decreased expression of ELF-3 indicating disturbed differentiation in oral lichen planus
2012 (English)In: Oral Diseases, ISSN 1354-523X, E-ISSN 1601-0825, Vol. 18, no Special Issue, Suppl. 1, p. 20-20Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
Wiley-Blackwell, 2012
National Category
Dentistry
Identifiers
urn:nbn:se:umu:diva-60505 (URN)000308718600091 ()
Available from: 2012-10-17 Created: 2012-10-15 Last updated: 2018-06-08Bibliographically approved
Danielsson, K., Ebrahimi, M., Wahlin, Y.-B., Nylander, K. & Boldrup, L. (2012). Increased levels of COX-2 in oral lichen planus supports an autoimmune cause of the disease. Journal of the European Academy of Dermatology and Venereology, 26(11), 1415-1419
Open this publication in new window or tab >>Increased levels of COX-2 in oral lichen planus supports an autoimmune cause of the disease
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2012 (English)In: Journal of the European Academy of Dermatology and Venereology, ISSN 0926-9959, E-ISSN 1468-3083, Vol. 26, no 11, p. 1415-1419Article in journal (Refereed) Published
Abstract [en]

Background: Oral lichen planus (OLP) is a chronic inflammatory disease for which the pathogenesis is not fully understood. OLP has autoimmune features and auto immunity has been suggested as a potential cause, whereas WHO has classified OLP as a premalignant condition. Association between chronic inflammation and cancer is known and chronic inflammation is one of the characteristics of OLP. A protein connected to inflammation and suggested to be involved in cancer development is cyclooxygenase-2 (COX-2) which can be inhibited by microRNA-26b (miR-26b).

Objective: The aim was to map levels of COX-2 and miR-26b in OLP lesions to see if there was any correlation between expression of COX-2 and its regulator miR-26b in OLP.

Methods: In biopsies from 20 OLP patients and 20 age and gender-matched controls laser- micro dissection of epithelium was performed. Quantitative RT-PCR, immunohistochemistry and Western blot were used in the analysis.

Results: Levels of COX-2 mRNA were significantly higher while levels of miR-26b were significantly lower in OLP lesions compared to controls. Using immunohistochemistry normal oral mucosa samples did not show any expression of COX-2 while OLP samples expressed the protein. No COX-2 protein was detectable with Western blot.

Conclusion: Increased expression of COX-2 and decreased expression of miR-26b in OLP suggests both to play a role in OLP. COX-2 has been connected to both malignant development and autoimmunity but as malignant development of OLP is quite rare we suggest that the increased levels of COX-2 seen here support an autoimmune cause of the disease.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2012
Keywords
Oral lichen planus, MicroRNA, Cox-2
National Category
Dentistry Dermatology and Venereal Diseases
Research subject
Pathology; Odontology
Identifiers
urn:nbn:se:umu:diva-48570 (URN)10.1111/j.1468-3083.2011.04306.x (DOI)000310271000013 ()22017396 (PubMedID)
Available from: 2011-10-25 Created: 2011-10-25 Last updated: 2018-06-08Bibliographically approved
Ebrahimi, M., Lundqvist, L., Wahlin, Y.-B. & Nylander, E. (2012). Mucosal lichen planus a systemic disease requiring multidisciplinary care. Oral Diseases, 18(Special Issue, Suppl. 1), 21-21
Open this publication in new window or tab >>Mucosal lichen planus a systemic disease requiring multidisciplinary care
2012 (English)In: Oral Diseases, ISSN 1354-523X, E-ISSN 1601-0825, Vol. 18, no Special Issue, Suppl. 1, p. 21-21Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
Wiley-Blackwell, 2012
National Category
Dentistry
Identifiers
urn:nbn:se:umu:diva-60506 (URN)000308718600094 ()
Available from: 2012-10-17 Created: 2012-10-15 Last updated: 2018-06-08Bibliographically approved
Ebrahimi, M., Lundqvist, L., Wahlin, Y. B. & Nylander, E. (2012). Mucosal lichen planus, a systemic disease requiring multidisciplinary care: a cross-sectional clinical review from a multidisciplinary perspective. Journal of Lower Genital Tract Disease, 16(4), 377-380
Open this publication in new window or tab >>Mucosal lichen planus, a systemic disease requiring multidisciplinary care: a cross-sectional clinical review from a multidisciplinary perspective
2012 (English)In: Journal of Lower Genital Tract Disease, ISSN 1089-2591, E-ISSN 1526-0976, Vol. 16, no 4, p. 377-380Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: This study aimed to emphasize the importance of seeing mucosal lichen planus (LP) as a systemic disease and not an isolated oral or genital disease and to analyze the proportion of thyroid antibodies among patients with multimucosal LP.

MATERIALS AND METHODS: All patients examined by the authors and diagnosed with mucosal LP within 1 year were consecutively included. Full medical histories were collected with special emphasis on autoimmune and thyroid diseases. Sera were analyzed for thyroid antibodies and underwent serologic test for herpes virus. The control group comprised 83 healthy volunteers matched regarding sex and age.

RESULTS: Of the patients, 120 were included, 89 (74%) of whom were women and 31 (26%) were men. The vast majority of the patients had multifocal lesions, whereas oral lesions solely were found in 28% of women and 36% of men. Of the patients, 28% had at least 1 additional autoimmune disease. Approximately half of the women were treated with levothyroxine owing to thyroid disease. Antibodies against herpes simplex virus were found in 60% of the patients and 44% of the controls (p < .03).

CONCLUSIONS: Lichen planus with mucosal involvement should be considered and taken care of as a systemic disease and not as an isolated oral and/or genital lichen. Contradictory to many former reports, most of our patients have a multimucosal disease that emphasizes the need for a multidisciplinary clinic to get optimal care and treatment.

National Category
Dentistry Dermatology and Venereal Diseases
Identifiers
urn:nbn:se:umu:diva-55770 (URN)10.1097/LGT.0b013e318247a907 (DOI)000309326200008 ()22622344 (PubMedID)
Available from: 2012-05-30 Created: 2012-05-30 Last updated: 2018-06-08Bibliographically approved
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