Umeå University's logo

umu.sePublications
Change search
Link to record
Permanent link

Direct link
Alternative names
Publications (10 of 120) Show all publications
Capo, E., Peterson, B. D., Kim, M., Jones, D. S., Acinas, S. G., Amyot, M., . . . Gionfriddo, C. M. (2023). A consensus protocol for the recovery of mercury methylation genes from metagenomes. Molecular Ecology Resources, 23(1), 190-204
Open this publication in new window or tab >>A consensus protocol for the recovery of mercury methylation genes from metagenomes
Show others...
2023 (English)In: Molecular Ecology Resources, ISSN 1755-098X, E-ISSN 1755-0998, Vol. 23, no 1, p. 190-204Article in journal (Refereed) Published
Abstract [en]

Mercury (Hg) methylation genes (hgcAB) mediate the formation of the toxic methylmercury and have been identified from diverse environments, including freshwater and marine ecosystems, Arctic permafrost, forest and paddy soils, coal-ash amended sediments, chlor-alkali plants discharges and geothermal springs. Here we present the first attempt at a standardized protocol for the detection, identification and quantification of hgc genes from metagenomes. Our Hg-cycling microorganisms in aquatic and terrestrial ecosystems (Hg-MATE) database, a catalogue of hgc genes, provides the most accurate information to date on the taxonomic identity and functional/metabolic attributes of microorganisms responsible for Hg methylation in the environment. Furthermore, we introduce “marky-coco”, a ready-to-use bioinformatic pipeline based on de novo single-metagenome assembly, for easy and accurate characterization of hgc genes from environmental samples. We compared the recovery of hgc genes from environmental metagenomes using the marky-coco pipeline with an approach based on coassembly of multiple metagenomes. Our data show similar efficiency in both approaches for most environments except those with high diversity (i.e., paddy soils) for which a coassembly approach was preferred. Finally, we discuss the definition of true hgc genes and methods to normalize hgc gene counts from metagenomes.

Place, publisher, year, edition, pages
John Wiley & Sons, 2023
Keywords
bioinformatics, hg methylation, hg-MATE, hgcAB genes, marky-coco, mercury, metagenomics
National Category
Ecology Environmental Sciences
Identifiers
urn:nbn:se:umu:diva-198622 (URN)10.1111/1755-0998.13687 (DOI)000836019100001 ()35839241 (PubMedID)2-s2.0-85135534585 (Scopus ID)
Funder
Swedish Research Council Formas, 2018-01031
Available from: 2022-08-15 Created: 2022-08-15 Last updated: 2022-12-30Bibliographically approved
Yunda, E., Phan Le, Q. N., Björn, E. & Ramstedt, M. (2023). Biochemical characterization and mercury methylation capacity of Geobacter sulfurreducens biofilms grown in media containing iron hydroxide or fumarate. Biofilm, 6, Article ID 100144.
Open this publication in new window or tab >>Biochemical characterization and mercury methylation capacity of Geobacter sulfurreducens biofilms grown in media containing iron hydroxide or fumarate
2023 (English)In: Biofilm, E-ISSN 2590-2075, Vol. 6, article id 100144Article in journal (Refereed) Published
Abstract [en]

Geobacter species are common in iron-rich environments and can contribute to formation of methylmercury (MeHg), a neurotoxic compound with high bioaccumulation potential formed as a result of bacterial and archaeal physiological activity. Geobacter sulfurreducens can utilize various electron acceptors for growth including iron hydroxides or fumarate. However, it remains poorly understood how the growth on these compounds affects physiological properties of bacterial cells in biofilms, including the capacity to produce MeHg. The purpose of this study was to determine changes in the biochemical composition of G. sulfurreducens during biofilm cultivation in media containing iron hydroxide or fumarate, and to quantify mercury (Hg) methylation capacity of the formed biofilms. Biofilms were characterized by Fourier-transform infrared spectroscopy in the attenuated total reflection mode (ATR-FTIR), Resonance Raman spectroscopy and confocal laser scanning microscopy. MeHg formation was quantified by mass spectrometry after incubation of biofilms with 100 nM Hg. The results of ATR-FTIR experiments showed that in presence of fumarate, G. sulfurreducens biofilm formation was accompanied by variation in content of the energy-reserve polymer glycogen over time, which could be cancelled by the addition of supplementary nutrients (yeast extract). In contrast, biofilms cultivated on Fe(III) hydroxide did not accumulate glycogen. The ATR-FTIR results further suggested that Fe(III) hydroxide surfaces bind cells via phosphate and carboxylate groups of bacteria that form complexes with iron. Furthermore, biofilms grown on Fe(III) hydroxide had higher fraction of oxidized cytochromes and produced two to three times less biomass compared to conditions with fumarate. Normalized to biofilm volume, the content of MeHg was similar in assays with biofilms grown on Fe(III) hydroxide and on fumarate (with yeast extract and without). These results suggest that G. sulfurreducens biofilms produce MeHg irrespectively from glycogen content and cytochrome redox state in the cells, and warrant further investigation of the mechanisms controlling this process.

Place, publisher, year, edition, pages
Elsevier, 2023
National Category
Biochemistry and Molecular Biology Analytical Chemistry
Identifiers
urn:nbn:se:umu:diva-213182 (URN)10.1016/j.bioflm.2023.100144 (DOI)001061813000001 ()37583615 (PubMedID)2-s2.0-85169894143 (Scopus ID)
Funder
The Kempe Foundations, JCK-1917
Available from: 2023-08-22 Created: 2023-08-22 Last updated: 2023-09-20Bibliographically approved
Seelen, E., Liem-Nguyen, V., Wünsch, U., Baumann, Z., Mason, R., Skyllberg, U. & Björn, E. (2023). Dissolved organic matter thiol concentrations determine methylmercury bioavailability across the terrestrial-marine aquatic continuum. Nature Communications, 14(1), Article ID 6728.
Open this publication in new window or tab >>Dissolved organic matter thiol concentrations determine methylmercury bioavailability across the terrestrial-marine aquatic continuum
Show others...
2023 (English)In: Nature Communications, E-ISSN 2041-1723, Vol. 14, no 1, article id 6728Article in journal (Refereed) Published
Abstract [en]

The most critical step for methylmercury (MeHg) bioaccumulation in aquatic food webs is phytoplankton uptake of dissolved MeHg. Dissolved organic matter (DOM) has been known to influence MeHg uptake, but the mechanisms have remained unclear. Here we show that the concentration of DOM-associated thiol functional groups (DOM-RSH) varies substantially across contrasting aquatic systems and dictates MeHg speciation and bioavailability to phytoplankton. Across our 20 study sites, DOM-RSH concentrations decrease 40-fold from terrestrial to marine environments whereas dissolved organic carbon (DOC), the typical proxy for MeHg binding sites in DOM, only has a 5-fold decrease. MeHg accumulation into phytoplankton is shown to be directly linked to the concentration of specific MeHg binding sites (DOM-RSH), rather than DOC. Therefore, MeHg bioavailability increases systematically across the terrestrial-marine aquatic continuum as the DOM-RSH concentration decreases. Our results strongly suggest that measuring DOM-RSH concentrations will improve empirical models in phytoplankton uptake studies and will form a refined basis for modeling MeHg incorporation in aquatic food webs under various environmental conditions.

Place, publisher, year, edition, pages
Springer Nature, 2023
National Category
Oceanography, Hydrology and Water Resources
Identifiers
urn:nbn:se:umu:diva-215944 (URN)10.1038/s41467-023-42463-4 (DOI)2-s2.0-85159600920 (Scopus ID)
Funder
Swedish Research Council, 2016-06459The Kempe Foundations, SMK-1243The Kempe Foundations, SMK-2745
Available from: 2023-11-01 Created: 2023-11-01 Last updated: 2023-11-01Bibliographically approved
Sonke, J. E., Angot, H., Zhang, Y., Poulain, A., Björn, E. & Schartup, A. (2023). Global change effects on biogeochemical mercury cycling. Ambio, 52, 853-876
Open this publication in new window or tab >>Global change effects on biogeochemical mercury cycling
Show others...
2023 (English)In: Ambio, ISSN 0044-7447, E-ISSN 1654-7209, Vol. 52, p. 853-876Article, review/survey (Refereed) Published
Abstract [en]

Past and present anthropogenic mercury (Hg) release to ecosystems causes neurotoxicity and cardiovascular disease in humans with an estimated economic cost of $117 billion USD annually. Humans are primarily exposed to Hg via the consumption of contaminated freshwater and marine fish. The UNEP Minamata Convention on Hg aims to curb Hg release to the environment and is accompanied by global Hg monitoring efforts to track its success. The biogeochemical Hg cycle is a complex cascade of release, dispersal, transformation and bio-uptake processes that link Hg sources to Hg exposure. Global change interacts with the Hg cycle by impacting the physical, biogeochemical and ecological factors that control these processes. In this review we examine how global change such as biome shifts, deforestation, permafrost thaw or ocean stratification will alter Hg cycling and exposure. Based on past declines in Hg release and environmental levels, we expect that future policy impacts should be distinguishable from global change effects at the regional and global scales.

Place, publisher, year, edition, pages
Springer, 2023
Keywords
Climate change, Environment, Exposure, Fish consumption, Minamata Convention, Toxicity
National Category
Environmental Sciences Other Chemistry Topics Ecology
Identifiers
urn:nbn:se:umu:diva-206785 (URN)10.1007/s13280-023-01855-y (DOI)000960249600001 ()36988895 (PubMedID)2-s2.0-85151614239 (Scopus ID)
Funder
EU, Horizon 2020, 860497
Available from: 2023-04-20 Created: 2023-04-20 Last updated: 2023-04-20Bibliographically approved
Li, C., Jiskra, M., Nilsson, M. B., Osterwalder, S., Zhu, W., Mauquoy, D., . . . Bishop, K. (2023). Mercury deposition and redox transformation processes in peatland constrained by mercury stable isotopes. Nature Communications, 14(1), Article ID 7389.
Open this publication in new window or tab >>Mercury deposition and redox transformation processes in peatland constrained by mercury stable isotopes
Show others...
2023 (English)In: Nature Communications, E-ISSN 2041-1723, Vol. 14, no 1, article id 7389Article in journal (Refereed) Published
Abstract [en]

Peatland vegetation takes up mercury (Hg) from the atmosphere, typically contributing to net production and export of neurotoxic methyl-Hg to downstream ecosystems. Chemical reduction processes can slow down methyl-Hg production by releasing Hg from peat back to the atmosphere. The extent of these processes remains, however, unclear. Here we present results from a comprehensive study covering concentrations and isotopic signatures of Hg in an open boreal peatland system to identify post-depositional Hg redox transformation processes. Isotope mass balances suggest photoreduction of HgII is the predominant process by which 30% of annually deposited Hg is emitted back to the atmosphere. Isotopic analyses indicate that above the water table, dark abiotic oxidation decreases peat soil gaseous Hg0 concentrations. Below the water table, supersaturation of gaseous Hg is likely created more by direct photoreduction of rainfall rather than by reduction and release of Hg from the peat soil. Identification and quantification of these light-driven and dark redox processes advance our understanding of the fate of Hg in peatlands, including the potential for mobilization and methylation of HgII.

Place, publisher, year, edition, pages
Nature Publishing Group, 2023
National Category
Geochemistry Environmental Sciences
Identifiers
urn:nbn:se:umu:diva-217026 (URN)10.1038/s41467-023-43164-8 (DOI)2-s2.0-85176776691 (Scopus ID)
Funder
Swedish Research Council, 2018-04695
Available from: 2023-11-23 Created: 2023-11-23 Last updated: 2023-11-24Bibliographically approved
Bouchet, S., Soerensen, A. L., Björn, E., Tessier, E. & Amouroux, D. (2023). Mercury sources and fate in a large brackish ecosystem (the Baltic Sea) depicted by stable isotopes. Environmental Science and Technology, 57(38), 14340-14350
Open this publication in new window or tab >>Mercury sources and fate in a large brackish ecosystem (the Baltic Sea) depicted by stable isotopes
Show others...
2023 (English)In: Environmental Science and Technology, ISSN 0013-936X, E-ISSN 1520-5851, Vol. 57, no 38, p. 14340-14350Article in journal (Refereed) Published
Abstract [en]

Identifying Hg sources to aquatic ecosystems and processes controlling the levels of monomethylmercury (MMHg) is critical for developing efficient policies of Hg emissions reduction. Here we measured Hg concentrations and stable isotopes in sediment, seston, and fishes from the various basins of the Baltic Sea, a large brackish ecosystem presenting extensive gradients in salinity, redox conditions, dissolved organic matter (DOM) composition, and biological activities. We found that Hg mass dependent fractionation (Hg-MDF) values in sediments mostly reflect a mixing between light terrestrial Hg and heavier industrial sources, whereas odd Hg isotope mass independent fractionation (odd Hg-MIF) reveals atmospheric inputs. Seston presents intermediate Hg-MDF and odd Hg-MIF values falling between sediments and fish, but in northern basins, high even Hg-MIF values suggest the preferential accumulation of wet-deposited Hg. Odd Hg-MIF values in fish indicate an overall low extent of MMHg photodegradation due to limited sunlight exposure and penetration but also reveal large spatial differences. The photodegradation extent is lowest in the central basin with recurrent algal blooms due to their shading effect and is highest in the northern, least saline basin with high concentrations of terrestrial DOM. As increased loads of terrestrial DOM are expected in many coastal areas due to global changes, its impact on MMHg photodegradation needs to be better understood and accounted for when predicting future MMHg concentrations in aquatic ecosystems.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2023
Keywords
dissolved organic matter, Hg sources, Hg stable isotopes, MMHg photodegradation
National Category
Environmental Sciences
Identifiers
urn:nbn:se:umu:diva-215088 (URN)10.1021/acs.est.3c03459 (DOI)001066353700001 ()37698522 (PubMedID)2-s2.0-85172425477 (Scopus ID)
Funder
Swedish Research Council Formas, 2014-1088Swedish Research Council Formas, 2021-00942
Available from: 2023-10-13 Created: 2023-10-13 Last updated: 2023-10-13Bibliographically approved
Bouchet, S., Soerensen, A. L., Björn, E., Tessier, E. & Amouroux, D. (2023). Mercury sources and fate in a large brackish ecosystem (The Baltic Sea) depicted by stable isotopes. Environmental Science and Technology, 57(38), 14340-14350
Open this publication in new window or tab >>Mercury sources and fate in a large brackish ecosystem (The Baltic Sea) depicted by stable isotopes
Show others...
2023 (English)In: Environmental Science and Technology, ISSN 0013-936X, E-ISSN 1520-5851, Vol. 57, no 38, p. 14340-14350Article in journal (Refereed) Published
Abstract [en]

Identifying Hg sources to aquatic ecosystems and processes controlling the levels of monomethylmercury (MMHg) is critical for developing efficient policies of Hg emissions reduction. Here we measured Hg concentrations and stable isotopes in sediment, seston, and fishes from the various basins of the Baltic Sea, a large brackish ecosystem presenting extensive gradients in salinity, redox conditions, dissolved organic matter (DOM) composition, and biological activities. We found that Hg mass dependent fractionation (Hg-MDF) values in sediments mostly reflect a mixing between light terrestrial Hg and heavier industrial sources, whereas odd Hg isotope mass independent fractionation (odd Hg-MIF) reveals atmospheric inputs. Seston presents intermediate Hg-MDF and odd Hg-MIF values falling between sediments and fish, but in northern basins, high even Hg-MIF values suggest the preferential accumulation of wet-deposited Hg. Odd Hg-MIF values in fish indicate an overall low extent of MMHg photodegradation due to limited sunlight exposure and penetration but also reveal large spatial differences. The photodegradation extent is lowest in the central basin with recurrent algal blooms due to their shading effect and is highest in the northern, least saline basin with high concentrations of terrestrial DOM. As increased loads of terrestrial DOM are expected in many coastal areas due to global changes, its impact on MMHg photodegradation needs to be better understood and accounted for when predicting future MMHg concentrations in aquatic ecosystems.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2023
Keywords
dissolved organic matter, Hg sources, Hg stable isotopes, MMHg photodegradation
National Category
Environmental Sciences
Identifiers
urn:nbn:se:umu:diva-214988 (URN)10.1021/acs.est.3c03459 (DOI)001066353700001 ()37698522 (PubMedID)2-s2.0-85172425477 (Scopus ID)
Funder
Swedish Research Council Formas, 2014-1088Swedish Research Council Formas, 2021-00942
Available from: 2023-10-13 Created: 2023-10-13 Last updated: 2023-10-13Bibliographically approved
Gutensohn, M., Schaefer, J. K., Maas, T. J., Skyllberg, U. & Björn, E. (2023). Metabolic turnover of cysteine-related thiol compounds at environmentally relevant concentrations by Geobacter sulfurreducens. Frontiers in Microbiology, 13, Article ID 1085214.
Open this publication in new window or tab >>Metabolic turnover of cysteine-related thiol compounds at environmentally relevant concentrations by Geobacter sulfurreducens
Show others...
2023 (English)In: Frontiers in Microbiology, E-ISSN 1664-302X, Vol. 13, article id 1085214Article in journal (Refereed) Published
Abstract [en]

Low-molecular-mass (LMM) thiol compounds are known to be important for many biological processes in various organisms but LMM thiols are understudied in anaerobic bacteria. In this work, we examined the production and turnover of nanomolar concentrations of LMM thiols with a chemical structure related to cysteine by the model iron-reducing bacterium Geobacter sulfurreducens. Our results show that G. sulfurreducens tightly controls the production, excretion and intracellular concentration of thiols depending on cellular growth state and external conditions. The production and cellular export of endogenous cysteine was coupled to the extracellular supply of Fe(II), suggesting that cysteine excretion may play a role in cellular trafficking to iron proteins. Addition of excess exogenous cysteine resulted in a rapid and extensive conversion of cysteine to penicillamine by the cells. Experiments with added isotopically labeled cysteine confirmed that penicillamine was formed by a dimethylation of the C-3 atom of cysteine and not via indirect metabolic responses to cysteine exposure. This is the first report of de novo metabolic synthesis of this compound. Penicillamine formation increased with external exposure to cysteine but the compound did not accumulate intracellularly, which may suggest that it is part of G. sulfurreducens’ metabolic strategy to maintain cysteine homeostasis. Our findings highlight and expand on processes mediating homeostasis of cysteine-like LMM thiols in strict anaerobic bacteria. The formation of penicillamine is particularly noteworthy and this compound warrants more attention in microbial metabolism studies.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2023
Keywords
anaerobe bacteria, cysteine homeostasis, Geobacter sulfurreducens, low-molecular-mass thiols, penicillamine formation
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-204469 (URN)10.3389/fmicb.2022.1085214 (DOI)000920099300001 ()2-s2.0-85146997112 (Scopus ID)
Funder
Swedish Research Council, 2017–04537The Kempe Foundations, SMK-1753The Kempe Foundations, SMK-1243Umeå University
Available from: 2023-02-17 Created: 2023-02-17 Last updated: 2024-01-17Bibliographically approved
Yunda, E., Gutensohn, M., Ramstedt, M. & Björn, E. (2023). Methylmercury formation in biofilms of Geobacter sulfurreducens. Frontiers in Microbiology, 14, Article ID 1079000.
Open this publication in new window or tab >>Methylmercury formation in biofilms of Geobacter sulfurreducens
2023 (English)In: Frontiers in Microbiology, E-ISSN 1664-302X, Vol. 14, article id 1079000Article in journal (Refereed) Published
Abstract [en]

Introduction: Mercury (Hg) is a major environmental pollutant that accumulates in biota predominantly in the form of methylmercury (MeHg). Surface-associated microbial communities (biofilms) represent an important source of MeHg in natural aquatic systems. In this work, we report MeHg formation in biofilms of the iron-reducing bacterium Geobacter sulfurreducens.

Methods: Biofilms were prepared in media with varied nutrient load for 3, 5, or 7 days, and their structural properties were characterized using confocal laser scanning microscopy, cryo-scanning electron microscopy and Fourier-transform infrared spectroscopy.

Results: Biofilms cultivated for 3 days with vitamins in the medium had the highest surface coverage, and they also contained abundant extracellular matrix. Using 3 and 7-days-old biofilms, we demonstrate that G. sulfurreducens biofilms prepared in media with various nutrient load produce MeHg, of which a significant portion is released to the surrounding medium. The Hg methylation rate constant determined in 6-h assays in a low-nutrient assay medium with 3-days-old biofilms was 3.9 ± 2.0 ∙ 10−14  L ∙ cell−1 ∙ h−1, which is three to five times lower than the rates found in assays with planktonic cultures of G. sulfurreducens in this and previous studies. The fraction of MeHg of total Hg within the biofilms was, however, remarkably high (close to 50%), and medium/biofilm partitioning of inorganic Hg (Hg(II)) indicated low accumulation of Hg(II) in biofilms.

Discussion: These findings suggest a high Hg(II) methylation capacity of G. sulfurreducens biofilms and that Hg(II) transfer to the biofilm is the rate-limiting step for MeHg formation in this systems.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2023
Keywords
methylmercury, biofilms, Geobacter sulfurreducens, mercury methylation, methylation rate
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-204111 (URN)10.3389/fmicb.2023.1079000 (DOI)000920863400001 ()2-s2.0-85147124881 (Scopus ID)
Funder
The Kempe Foundations, JCK-1917
Available from: 2023-01-27 Created: 2023-01-27 Last updated: 2024-01-17Bibliographically approved
Wang, B., Hu, H., Bishop, K., Buck, M., Björn, E., Skyllberg, U., . . . Bravo, A. G. (2023). Microbial communities mediating net methylmercury formation along a trophic gradient in a peatland chronosequence. Journal of Hazardous Materials, 442, Article ID 130057.
Open this publication in new window or tab >>Microbial communities mediating net methylmercury formation along a trophic gradient in a peatland chronosequence
Show others...
2023 (English)In: Journal of Hazardous Materials, ISSN 0304-3894, E-ISSN 1873-3336, Vol. 442, article id 130057Article in journal (Refereed) Published
Abstract [en]

Peatlands are generally important sources of methylmercury (MeHg) to adjacent aquatic ecosystems, increasing the risk of human and wildlife exposure to this highly toxic compound. While microorganisms play important roles in mercury (Hg) geochemical cycles where they directly and indirectly affect MeHg formation in peatlands, potential linkages between net MeHg formation and microbial communities involving these microorganisms remain unclear. To address this gap, microbial community composition and specific marker gene transcripts were investigated along a trophic gradient in a geographically constrained peatland chronosequence. Our results showed a clear spatial pattern in microbial community composition along the gradient that was highly driven by peat soil properties and significantly associated with net MeHg formation as approximated by MeHg concentration and %MeHg of total Hg concentration. Known fermentative, syntrophic, methanogenic and iron-reducing metabolic guilds had the strong positive correlations to net MeHg formation, while methanotrophic and methylotrophic microorganisms were negatively correlated. Our results indicated that sulfate reducers did not have a key role in net MeHg formation. Microbial activity as interpreted from 16S rRNA sequences was significantly correlated with MeHg and %MeHg. Our findings shed new light on the role of microbial community in net MeHg formation of peatlands that undergo ontogenetic change.

Keywords
MeHg, Mercury, Mercury methylation, Microbial Community, Peatland trophic gradient
National Category
Forest Science
Identifiers
urn:nbn:se:umu:diva-200070 (URN)10.1016/j.jhazmat.2022.130057 (DOI)000863097300001 ()2-s2.0-85138806376 (Scopus ID)
Funder
Swedish Research Council Formas, 2016–00896Swedish Research Council, 2013–697
Available from: 2022-10-11 Created: 2022-10-11 Last updated: 2023-09-05Bibliographically approved
Projects
Methyl mercury formation in aquatic systems with different pelagic food web structure and productivity - Novel strategies for molecular-level studies in mesocosm experiments [2008-04363_VR]; Umeå UniversityUnderstanding the possible re-activation of high priority contaminants release from pulp fibre sediments in the northern Baltic Sea (REACT) [2012-2090_Formas]; Umeå UniversityDoes climate change threaten fishery ecosystem services in the Baltic Sea via increased mercury contamination of biota? [2014-1088_Formas]; Umeå UniversityA molecular approach to understand bioavailability of methylmercury associated with variable sources of natural dissolved organic matter [2016-06459_VR]; Umeå UniversityMechanistic principles of mercury uptake by methylating bacteria - redefining the function of thiol compounds [2017-04537_VR]; Umeå University
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-9570-8738

Search in DiVA

Show all publications