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Mahmud, A K M Firoj
Publications (3 of 3) Show all publications
Taheri, N., Mahmud, A. K., Sandblad, L., Fällman, M., Wai, S. N. & Fahlgren, A. (2018). Campylobacter jejuni bile exposure influences outer membrane vesicles protein content and bacterial interaction with epithelial cells. Scientific Reports, 8, Article ID 16996.
Open this publication in new window or tab >>Campylobacter jejuni bile exposure influences outer membrane vesicles protein content and bacterial interaction with epithelial cells
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2018 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, article id 16996Article in journal (Refereed) Published
Abstract [en]

Campylobacter jejuni is a prevalent human pathogen and a major cause of bacterial gastroenteritis in the world. In humans, C. jejuni colonizes the intestinal tract and its tolerance to bile is crucial for bacteria to survive and establish infection. C. jejuni produces outer membrane vesicles (OMVs) which have been suggested to be involved in virulence. In this study, the proteome composition of C. jejuni OMVs in response to low concentration of bile was investigated. We showed that exposure of C. jejuni to low concentrations of bile, similar to the concentration in cecum, induced significant changes in the protein profile of OMVs released during growth without affecting the protein profile of the bacteria. This suggests that bile influences a selective packing of the OMVs after bacterial exposure to low bile. A low concentration of bile was found to increase bacterial adhesion to intestinal epithelial cells, likely by an enhanced hydrophobicity of the cell membrane following exposure to bile. The increased bacterial adhesiveness was not associated with increased invasion, instead bile exposure decreased C. jejuni invasion. OMVs released from bacteria upon exposure to low bile showed to increase both adhesion and invasion of non-bile-exposed bacteria into intestinal epithelial cells. These findings suggest that C. jejuni in environments with low concentrations of bile produce OMVs that facilitates colonization of the bacteria, and this could potentially contribute to virulence of C. jejuni in the gut.

Place, publisher, year, edition, pages
Nature Publishing Group, 2018
National Category
Microbiology Cell Biology
Identifiers
urn:nbn:se:umu:diva-153787 (URN)10.1038/s41598-018-35409-0 (DOI)000450411700027 ()
Funder
Carl Tryggers foundation
Available from: 2018-12-03 Created: 2018-12-03 Last updated: 2019-01-18Bibliographically approved
Karlsborn, T., Mahmud, A. K., Tükenmez, H. & Byström, A. S. (2016). Loss of ncm5 and mcm5 wobble uridine side chains results in an altered metabolic profile. Metabolomics, 12(12), Article ID 177.
Open this publication in new window or tab >>Loss of ncm5 and mcm5 wobble uridine side chains results in an altered metabolic profile
2016 (English)In: Metabolomics, ISSN 1573-3882, E-ISSN 1573-3890, Vol. 12, no 12, article id 177Article in journal (Refereed) Published
Abstract [en]

Introduction: The Elongator complex, comprising six subunits (Elp1p-Elp6p), is required for formation of 5-carbamoylmethyl (ncm(5)) and 5-methoxycarbonylmethyl (mcm(5)) side chains on wobble uridines in 11 out of 42 tRNA species in Saccharomyces cerevisiae. Loss of these side chains reduces the efficiency of tRNA decoding during translation, resulting in pleiotropic phenotypes. Overexpression of hypomodified tRNA(s2UUU)(Lys); tRNA(s2UUG)(Gln) and tRNA(s2UUC)(Glu), which in wild-type strains are modified with mcm(5)s(2)U, partially suppress phenotypes of an elp3 Delta strain. Objectives: To identify metabolic alterations in an elp3 Delta strain and elucidate whether these metabolic alterations are suppressed by overexpression of hypomodified tRNA(s2UUU)(Lys); tRNA(s2UUG)(Gln) and tRNA(s2UUC)(Glu). Method: Metabolic profiles were obtained using untargeted GC-TOF-MS of a temperature-sensitive elp3 Delta strain carrying either an empty low-copy vector, an empty high-copy vector, a low-copy vector harboring the wild-type ELP3 gene, or a high-copy vector overexpressing tRNA(s2UUU)(Lys); tRNA(s2UUG)(Gln) and tRNA(s2UUC)(Glu). The temperature sensitive elp3 Delta strain derivatives were cultivated at permissive (30 degrees C) or semi-permissive (34 degrees C) growth conditions. Results: Culturing an elp3 Delta strain at 30 or 34 degrees C resulted in altered metabolism of 36 and 46 %, respectively, of all metabolites detected when compared to an elp3D strain carrying the wild-type ELP3 gene. Overexpression of hypomodified tRNA(s2UUU)(Lys); tRNA(s2UUG)(Gln) and tRNA(s2UUC)(Glu) suppressed a subset of the metabolic alterations observed in the elp3 Delta strain. Conclusion: Our results suggest that the presence of ncm(5)- and mcm(5)-side chains on wobble uridines in tRNA are important for metabolic homeostasis.

Place, publisher, year, edition, pages
Springer, 2016
Keywords
Elongator complex, tRNA wobble uridine modifications, Translation, ELP3, Metabolomics, Metabolic profiling
National Category
Biochemistry and Molecular Biology Endocrinology and Diabetes
Research subject
Molecular Biology
Identifiers
urn:nbn:se:umu:diva-125635 (URN)10.1007/s11306-016-1120-8 (DOI)000389604300002 ()27738410 (PubMedID)
Available from: 2016-09-13 Created: 2016-09-13 Last updated: 2018-06-07Bibliographically approved
Tükenmez, H., Karlsborn, T., Mahmud, A. K., Chen, C., Xu, F. & Byström, A. S.Elongator complex enhances Rnr1p levels in response to DNA damage by influencing Ixr1p expression.
Open this publication in new window or tab >>Elongator complex enhances Rnr1p levels in response to DNA damage by influencing Ixr1p expression
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(English)Manuscript (preprint) (Other academic)
National Category
Genetics
Research subject
Molecular Biology
Identifiers
urn:nbn:se:umu:diva-124966 (URN)
Available from: 2016-08-31 Created: 2016-08-31 Last updated: 2018-06-07
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