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Prittinen, J., Ylärinne, J., Piltti, J., Karhula, S. S., Rieppo, L., Ojanen, S. P., . . . Qu, C. (2019). Effect of centrifugal force on the development of articular neocartilage with bovine primary chondrocytes. Cell and Tissue Research, 375(3), 629-639
Open this publication in new window or tab >>Effect of centrifugal force on the development of articular neocartilage with bovine primary chondrocytes
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2019 (English)In: Cell and Tissue Research, ISSN 0302-766X, E-ISSN 1432-0878, Vol. 375, no 3, p. 629-639Article in journal (Refereed) Published
Abstract [en]

A lot has been invested into understanding how to assemble cartilage tissue in vitro and various designs have been developed to manufacture cartilage tissue with native-like biological properties. So far, no satisfactory design has been presented. Bovine primary chondrocytes are used to self-assemble scaffold-free constructs to investigate whether mechanical loading by centrifugal force would be useful in manufacturing cartilage tissue in vitro. Six million chondrocytes were laid on top of defatted bone disks placed inside an agarose well in 50-ml culture tubes. The constructs were centrifuged once or three times per day for 15 min at a centrifugal force of 771×g for up to 4 weeks. Control samples were cultured under the same conditions without exposure to centrifugation. The samples were analysed by (immuno)histochemistry, Fourier transform infrared imaging, micro-computed tomography, biochemical and gene expression analyses. Biomechanical testing was also performed. The centrifuged tissues had a more even surface covering a larger area of the bone disk. Fourier transform infrared imaging analysis indicated a higher concentration of collagen in the top and bottom edges in some of the centrifuged samples. Glycosaminoglycan contents increased along the culture, while collagen content remained at a rather constant level. Aggrecan and procollagen α1(II) gene expression levels had no significant differences, while procollagen α2(I) levels were increased significantly. Biomechanical analyses did not reveal remarkable changes. The centrifugation regimes lead to more uniform tissue constructs, whereas improved biological properties of the native tissue could not be obtained by centrifugation.

Place, publisher, year, edition, pages
New York: Springer, 2019
Keywords
Cartilage tissue engineering, Centrifugal force, Osteoarthritis, Primary chondrocyte, Tissue assembly
National Category
Cell and Molecular Biology Orthopaedics Biochemistry and Molecular Biology Cell Biology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Biochemistry; biomechanics; cell research; Orthopaedics
Identifiers
urn:nbn:se:umu:diva-152816 (URN)10.1007/s00441-018-2938-3 (DOI)000460535300006 ()30349935 (PubMedID)
Available from: 2018-11-08 Created: 2018-11-08 Last updated: 2019-04-08Bibliographically approved
Lin, X., Shao, W., Yu, F., Xing, K., Liu, H., Zhang, F., . . . Guo, X. (2019). Individual and combined toxicity of T-2 toxin and deoxynivalenol on human C-28/I2 and rat primary chondrocytes. Journal of Applied Toxicology, 39(2), 343-353, Article ID 30251759.
Open this publication in new window or tab >>Individual and combined toxicity of T-2 toxin and deoxynivalenol on human C-28/I2 and rat primary chondrocytes
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2019 (English)In: Journal of Applied Toxicology, ISSN 0260-437X, E-ISSN 1099-1263, Vol. 39, no 2, p. 343-353, article id 30251759Article in journal (Refereed) Published
Abstract [en]

Deoxynivalenol (DON) and T-2 toxin are prevalent mycotoxin contaminants in the food and feed stuffs worldwide, with non-negligible co-contamination and co-exposure conditions. Meanwhile, they are considerable risk factors for Kashin-Beck disease, a chronic endemic osteochondropathy. The aim of this study was to investigate the individual and combined cytotoxicity of DON and T-2 toxin on proliferating human C-28/I2 and newborn rat primary costal chondrocytes by MTT assay. Four molar concentration combination ratios of DON and T-2 toxin were used, 1:1 for R1 mixture, 10:1 for R10, 100:1 for R100 and 1000:1 for R1000. The toxicological interactions were quantified by the MixLow method. DON, T-2 toxin, and their mixtures all showed a clear dose-dependent toxicity for chondrocytes. The cytotoxicity of T-2 toxin was 285-fold higher than DON was in human chondrocytes, and 22-fold higher in the rat chondrocytes. The combination of DON and T-2 toxin was significantly synergistic at middle and high level concentrations of R10 mixtures in rat chondrocytes, but significantly antagonistic at the low concentrations of R100 mixtures in both cells and at the middle concentrations of R1000 mixtures in rat chondrocytes. These results indicated that the combined toxicity was influenced by the cell sensitivity for toxins, the difference between the combination ratio and equitoxic ratio, the concentrations and other factors.

Place, publisher, year, edition, pages
John Wiley & Sons, 2019
Keywords
T-2 toxin, antagonism, chondrocyte, combined toxicity, deoxynivalenol, synergism
National Category
Pharmacology and Toxicology Cell Biology Cell and Molecular Biology
Research subject
cellforskning; Toxicology
Identifiers
urn:nbn:se:umu:diva-152125 (URN)10.1002/jat.3725 (DOI)000456205000015 ()30251759 (PubMedID)
Available from: 2018-09-27 Created: 2018-09-27 Last updated: 2019-02-26Bibliographically approved
Liu, H., Yu, F., Shao, W., Ding, D., Yu, Z., Chen, F., . . . Guo, X. (2018). Associations between selenium content in hair and Kashin-Beck Disease/Keshan Disease in children in Northwestern China: a prospective cohort study. Biological Trace Element Research, 184(1), 16-23, Article ID 28983831.
Open this publication in new window or tab >>Associations between selenium content in hair and Kashin-Beck Disease/Keshan Disease in children in Northwestern China: a prospective cohort study
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2018 (English)In: Biological Trace Element Research, ISSN 0163-4984, E-ISSN 1559-0720, Vol. 184, no 1, p. 16-23, article id 28983831Article in journal (Refereed) Published
Abstract [en]

The objective of this study was to investigate the relationship between selenium content in hair and the incidence of Kashin-Beck disease (KBD) and Keshan disease (KD) in China. A prospective cohort study was conducted among children aged 5-12 years with different levels of low-selenium (group 1, Se ≤ 110 ng/g; group 2, 110 < Se ≤ 150 ng/g; and group 3, 150 < Se ≤ 200 ng/g) or selenium-supplemented (group 4, Se > 200 ng/g) exposure. A person-years approach was used to calculate the incidence and rate of positive clinical signs. Relative risk (RR), attributable risk, and etiologic fraction were used to determine the strength of association between selenium and disease incidence. Seven new KBD cases were diagnosed during 3-year follow-up. Positive clinical signs of KBD were found in 17.78 (95% confidence interval [CI] 14.27-21.29) cases per 100 person-years in group 1, 13.28 (9.82-16.74) in group 2, 12.95 (9.34-16.56) in group 3, and 8.18 (5.50-10.85) in group 4. Compared with group 4, the RR (95% CI) of groups 1, 2, and 3 were 2.17 (1.48-3.19), 1.62 (1.07-2.47), and 1.58 (1.03-2.43), respectively. Positive clinical signs of KD were 25.90 (18.62-33.18) cases per 100 person-years in group 1, 5.66 (1.26-10.06) in group 2, 4.60 (0.20-9.00) in group 3, and 14.62 (8.54-20.69) in group 4. Compared with group 4, the RR (95% CI) were 1.77 (1.07-2.93), 0.39 (0.16-0.93), and 0.31 (0.11-0.89), respectively. In children, the onset of KBD was negatively correlated with selenium content within a certain range. However, there may be a U-shaped association between selenium content and KD in children.

Place, publisher, year, edition, pages
Springer, 2018
Keywords
Cohort study, Kashin-Beck disease, Keshan disease, Selenium
National Category
Pediatrics Public Health, Global Health, Social Medicine and Epidemiology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Pediatrics
Identifiers
urn:nbn:se:umu:diva-140367 (URN)10.1007/s12011-017-1169-x (DOI)000434723900003 ()28983831 (PubMedID)
Available from: 2017-10-08 Created: 2017-10-08 Last updated: 2018-08-27Bibliographically approved
Lammi, M., Piltti, J., Prittinen, J. & Qu, C. (2018). Challenges in fabrication of tissue-engineered cartilage with correct cellular colonization and extracellular matrix assembly. International Journal of Molecular Sciences, 19(9), Article ID 2700.
Open this publication in new window or tab >>Challenges in fabrication of tissue-engineered cartilage with correct cellular colonization and extracellular matrix assembly
2018 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 19, no 9, article id 2700Article, review/survey (Refereed) Published
Abstract [en]

A correct articular cartilage ultrastructure regarding its structural components and cellularity is important for appropriate performance of tissue-engineered articular cartilage. Various scaffold-based, as well as scaffold-free, culture models have been under development to manufacture functional cartilage tissue. Even decellularized tissues have been considered as a potential choice for cellular seeding and tissue fabrication. Pore size, interconnectivity, and functionalization of the scaffold architecture can be varied. Increased mechanical function requires a dense scaffold, which also easily restricts cellular access within the scaffold at seeding. High pore size enhances nutrient transport, while small pore size improves cellular interactions and scaffold resorption. In scaffold-free cultures, the cells assemble the tissue completely by themselves; in optimized cultures, they should be able to fabricate native-like tissue. Decellularized cartilage has a native ultrastructure, although it is a challenge to obtain proper cellular colonization during cell seeding. Bioprinting can, in principle, provide the tissue with correct cellularity and extracellular matrix content, although it is still an open question as to how the correct molecular interaction and structure of extracellular matrix could be achieved. These are challenges facing the ongoing efforts to manufacture optimal articular cartilage.

Place, publisher, year, edition, pages
MDPI, 2018
Keywords
articular cartilage, cartilage architecture, cell colonization, extracellular matrix, tissue engineering
National Category
Cell and Molecular Biology Orthopaedics Biochemistry and Molecular Biology Cell Biology
Research subject
Biochemistry; cellforskning; Orthopaedics
Identifiers
urn:nbn:se:umu:diva-151888 (URN)10.3390/ijms19092700 (DOI)000449988100236 ()30208585 (PubMedID)2-s2.0-85053359968 (Scopus ID)
Available from: 2018-09-16 Created: 2018-09-16 Last updated: 2018-12-18Bibliographically approved
Li, W., Hirvasniemi, J., Guo, X., Saarakkala, S., Lammi, M. & Qu, C. (2018). Comparison of bone texture between normal individuals and patients with Kashin-Beck disease from plain radiographs in knee. Scientific Reports, 8, Article ID 17510.
Open this publication in new window or tab >>Comparison of bone texture between normal individuals and patients with Kashin-Beck disease from plain radiographs in knee
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2018 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, article id 17510Article in journal (Refereed) Published
Abstract [en]

To compare tibial bone texture between Kashin-Beck disease (KBD) patients and normal individuals from plain radiographs using an advanced image analysis. Plain knee radiographs were obtained from KBD patients (n = 49) and age-matched healthy controls (n = 98). KBD were graded with diagnostic criteria WS/T 207-2010. The textural values related to bone structure from medial and lateral tibial subchondral and trabecular bones were evaluated using entropy of Laplacian-based image (ELap), entropy of local binary patterns (ELBP), homogeneity indices (HI) of local angles (HIMean, HIPerp and HIParal), and fractal dimensions from horizontal (FDHor) and vertical (FDVer) structures. KBD patients were shorter in height and lighter in weight, and their tibial width was wider than controls. Anatomical angle of KBD patients showed more genu valgus. Total KBD patients and subgroups had higher ELap, HIMean, HIPerp and HIParal in detected tibial subchondral and trabecular bones than controls, except ELap in lateral subchondral bone. ELBP, FDHor and FDVer from the detected tibial bone in KBD patients and subgroups were lower than controls, except FDVer in lateral trabecular bone. Our results indicate that micro-scale in bone texture in KBD-affected knees can be quantitatively examined from plain radiographs using an advanced image analysis.

Place, publisher, year, edition, pages
Nature Publishing Group, 2018
Keywords
Kashin-beck disease, Radiology, X-ray, bone texture
National Category
Orthopaedics Radiology, Nuclear Medicine and Medical Imaging
Research subject
Computerized Image Analysis; Orthopaedics; Radiology
Identifiers
urn:nbn:se:umu:diva-153929 (URN)10.1038/s41598-018-35552-8 (DOI)000451748700032 ()30504816 (PubMedID)
Available from: 2018-12-09 Created: 2018-12-09 Last updated: 2018-12-20Bibliographically approved
Piltti, J., Bygdell, J., Qu, C. & Lammi, M. (2018). Effects of long-term hypoxia in human chondrosarcoma cells. Journal of Cellular Biochemistry, 119(2), 2320-2332
Open this publication in new window or tab >>Effects of long-term hypoxia in human chondrosarcoma cells
2018 (English)In: Journal of Cellular Biochemistry, ISSN 0730-2312, E-ISSN 1097-4644, Vol. 119, no 2, p. 2320-2332Article in journal (Refereed) Published
Abstract [en]

The cell-based therapies could be potential methods to treat damaged cartilage tissues. Instead of native hyaline cartilage, the current therapies generate mainly weaker fibrocartilage-type of repair tissue. A correct microenvironment influences the cellular phenotype, and together with external factors it can be used, e.g., to aid the differentiation of mesenchymal stem cells to defined types of differentiated adult cells. In this study, we investigated the effect of long-term exposure to 5% low oxygen atmosphere on human chondrosarcoma HCS-2/8 cells. This atmosphere is close to normal oxygen tension of cartilage tissue. The proteome was analyzed with label-free mass spectrometric method and further bioinformatic analysis. The qRT-PCR method was used to gene expression analysis, and ELISA and dimethylmethylene blue assays for type II collagen and sulfated glycosaminoglycan measurements. The hypoxic atmosphere did not influence cell proliferation, but enhanced slightly ACAN and COL2A1 gene expression. Proteomic screening revealed a number of hypoxia-induced protein level responses. Increased ones included NDUFA4L2, P4HA1, NDRG1, MIF, LDHA, PYGL, while TXNRD1, BAG2, TXN2, AQSTM1, TNFRSF1B and EPHX1 decreased during the long-term hypoxia. Also a number of proteins previously not related to hypoxia changed during the treatment. Of those S100P, RPSS26, NDUFB11, CDV3 and TUBB8 had elevated levels, while ALCAM, HLA-B, EIF1, and ACOT9 were lower in the hypoxia samples. In conclusion, low oxygen condition causes changes in the cellular amounts of several proteins.

Place, publisher, year, edition, pages
John Wiley & Sons, 2018
Keywords
chondrosarcoma, extracellular matrix, hypoxia, label-free quantitative proteomics, S100 proteins
National Category
Cell Biology Cell and Molecular Biology
Research subject
Biochemistry; cellforskning; Medical Cell Biology
Identifiers
urn:nbn:se:umu:diva-138929 (URN)10.1002/jcb.26394 (DOI)000418708300098 ()28865129 (PubMedID)
Funder
Swedish Rheumatism Association, R-567071
Available from: 2017-09-04 Created: 2017-09-04 Last updated: 2018-12-18Bibliographically approved
Ning, Y., Wang, X., Zhang, P., Anatoly, S. V., Prakash, N. T., Li, C., . . . Guo, X. (2018). Imbalance of dietary nutrients and the associated differentially expressed genes and pathways may play important roles in juvenile Kashin-Beck disease. Journal of Trace Elements in Medicine and Biology, 50, 441-460, Article ID 29426639.
Open this publication in new window or tab >>Imbalance of dietary nutrients and the associated differentially expressed genes and pathways may play important roles in juvenile Kashin-Beck disease
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2018 (English)In: Journal of Trace Elements in Medicine and Biology, ISSN 0946-672X, E-ISSN 1878-3252, Vol. 50, p. 441-460, article id 29426639Article in journal (Refereed) Published
Abstract [en]

BACKGROUND:

Kashin-Beck disease (KBD) is a childhood-onset endemic osteoarthropathy in China. Nutrients including trace elements may play active roles in the development of KBD.

OBJECTIVE:

This study aimed to estimate the nutrient intakes of children in endemic areas and to identify the imbalanced nutrients associated differentially expressed genes in the juvenile patients with KBD.

METHODS:

In this cross-sectional study, a consecutive 3 day 24 h semi-quantitative dietary retrospect questionnaire was conducted to estimate the daily nutrient intakes of children using CDGSS 3.0 software. Gene profile analysis was employed to identify differentially expressed genes in peripheral blood mononuclear cells of children with KBD. GOC, CTD, KEGG, and REACTOME databases were used to establish the relationship between nutrients and nutrients-associated differentially expressed genes and pathways. Statistical analyses were accomplished by SPSS 18.0 software.

RESULTS:

Daily Se intakes without supplementation of children were significantly lower in Se-supplemented (Se + ) KBD areas (29.3 ∼ 29.6 mg/d) and non-endemic area (27.8 ± 7.9 mg/d) compared to non-Se-supplemented (Se-) KBD area (32.9 ± 7.9 mg/d, c2 = 20.24, P < .01). Children in Se+ KBD areas were suffering more serious insufficient intake of multiple nutrients, including vitamins-B2/-C/-E, Ca, Fe, Zn and I. Gene profile analysis combined with bioinformatics technique identified 34 nutrients associated differentially expressed genes and 10 significant pathways which are related to the pathological changes in juvenile KBD.

CONCLUSIONS:

Imbalance of dietary nutrients and nutrients-associated differentially expressed genes and pathways may play important roles in the development of juvenile KBD.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Children, Differentially expressed genes, Kashin-Beck disease, Nutrients, Selenium
National Category
Cell and Molecular Biology Nutrition and Dietetics
Research subject
cellforskning; Nutrition; Molecular Cellbiology
Identifiers
urn:nbn:se:umu:diva-147560 (URN)10.1016/j.jtemb.2018.01.012 (DOI)29426639 (PubMedID)
Available from: 2018-05-08 Created: 2018-05-08 Last updated: 2018-11-06Bibliographically approved
Wang, S., Lv, Y., Wang, Y., Du, P., Tan, W., Lammi, M. & Guo, X. (2018). Network analysis of Se-and Zn-related proteins in the serum proteomics expression profile of the endemic dilated cardiomyopathy Keshan disease. Biological Trace Element Research, 183(1), 40-48
Open this publication in new window or tab >>Network analysis of Se-and Zn-related proteins in the serum proteomics expression profile of the endemic dilated cardiomyopathy Keshan disease
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2018 (English)In: Biological Trace Element Research, ISSN 0163-4984, E-ISSN 1559-0720, Vol. 183, no 1, p. 40-48Article in journal (Refereed) Published
Abstract [en]

Keshan disease (KD) is an endemic cardiomyopathy with high mortality. Selenium (Se) and zinc (Zn) deficiencies are closely related to KD. The molecular mechanism of KD pathogenesis is still unclear. There are only few studies on the interaction of trace elements and proteins associated with the pathogenesis of KD. In this study, isobaric tags for relative and absolute quantitation (iTRAQ)-coupled two-dimensional liquid chromatography tandem mass spectrometry (2DLC-MS/MS) technique analysis was used to analyze the differential expression of proteins from serum samples. Comparative Toxicogenomics Database (CTD) was used to screen Se- and Zn-associated proteins. Then, pathway and network analyses of Se- and Zn-associated proteins were constituted by Cytoscape ClueGO and GeneMANIA plugins. One hundred and five differentially expressed proteins were obtained by 2DLC-MS/MS, among them 19 Se- and 3 Zn-associated proteins. Fifty-two pathways were identified from ClueGO and 1 network from GeneMANIA analyses. The results showed that Se-associated proteins STAT3 and MAPK1 and Zn-associated proteins HIF1A and PARP1, the proteins involved in HIF-1 signaling pathway and apoptosis pathway, may play significant roles in the pathogenesis of KD. The approach of this study would be also beneficial for further dissecting molecular mechanism of other trace element-associated disease.

Place, publisher, year, edition, pages
Springer, 2018
Keywords
Cardiomyocyte, GeneGO, GeneMANIA, Keshan disease, Trace element, iTRAQ
National Category
Cardiac and Cardiovascular Systems Biochemistry and Molecular Biology
Research subject
Biochemistry; Medicine, cardiovascular disease
Identifiers
urn:nbn:se:umu:diva-138316 (URN)10.1007/s12011-017-1063-6 (DOI)000429359900006 ()28819918 (PubMedID)
Available from: 2017-08-19 Created: 2017-08-19 Last updated: 2018-06-09Bibliographically approved
Ning, Y., Wang, X., Guo, X., Zhang, P., Qu, P., Zhang, F., . . . Lammi, M. (2018). Nutrients other than selenium are important for promoting children's health in Kashin-Beck disease areas. Biological Trace Element Research, 183(2), 233-244
Open this publication in new window or tab >>Nutrients other than selenium are important for promoting children's health in Kashin-Beck disease areas
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2018 (English)In: Biological Trace Element Research, ISSN 0163-4984, E-ISSN 1559-0720, Vol. 183, no 2, p. 233-244Article in journal (Refereed) Published
Abstract [en]

Overall nutritional status has been proved associated with people's health. The overall nutritional status of children in Kashin-Beck disease (KBD) areas has been overlooked for decades. Therefore, it is worth investigating in the current generation to gather evidence and make suggestions for improvement. A cross-sectional study with three 24-h dietary recalls was conducted to collect raw data on the daily food intake of children. Recorded food was converted into daily nutrient intakes using CDGSS 3.0 software. WHO AnthroPlus software was used to analyse the BMI-for-age z-score (BAZ) for estimating the overall nutrition status of children. All the comparisons and regression analyses were conducted with SPSS 18.0 software. Multiple nutrient intakes among children from the Se-supplemented KBD-endemic were under the estimated average requirement. The protein-to-carbohydrate ratio (P/C ratio) was significantly higher in children from the non-Se-supplemented KBD-endemic area than the other areas (< 0.001). The children's BAZ was negatively associated with age (B = -0.095, P < 0.001) and the number of KBD relatives (B = -0.277, P = 0.04), and it was positively associated with better housing conditions, receiving colostrum, and daily intakes of niacin and zinc by multivariate regression analysis (F = 10.337, R = 0.609, P < 0.001).Compared to non-Se-supplemented KBD-endemic area and non-endemic areas, children in Se-supplemented KBD-endemic areas have an insufficient intake of multiple nutrients. School breakfast and lunch programmes are recommended, and strict implementation is the key to ensuring a positive effect.

Place, publisher, year, edition, pages
Springer, 2018
Keywords
BMI-for-age-z-score, Children, Kashin-Beck disease, Nutrient intake, Overall nutrition
National Category
Nutrition and Dietetics Pediatrics
Research subject
Nutrition; Pediatrics
Identifiers
urn:nbn:se:umu:diva-139577 (URN)10.1007/s12011-017-1154-4 (DOI)000431689100007 ()28921450 (PubMedID)
Available from: 2017-09-19 Created: 2017-09-19 Last updated: 2018-06-09Bibliographically approved
Wang, S., Yan, R., Wang, B., Du, P., Tan, W., Lammi, M. J. & Guo, X. (2018). Prediction of co-expression genes and integrative analysis of gene microarray and proteomics profile of Keshan disease. Scientific Reports, 8, Article ID 231.
Open this publication in new window or tab >>Prediction of co-expression genes and integrative analysis of gene microarray and proteomics profile of Keshan disease
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2018 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, article id 231Article in journal (Refereed) Published
Abstract [en]

Keshan disease (KD) is a kind of endemic cardiomyopathy which has a high mortality. However, molecular mechanism in the pathogenesis of KD remains poorly understood. Serum samples were collected from 112 KD patients and 112 normal controls. Gene microarray was used to screen differently expressed genes. Genevestigator was applied to forecast co-expression genes of significant gene. iTRAQ proteomics analysis was used to verify significant genes and their co-expression genes. GO, COG, IPA and STRING were applied to undertake function categorization, pathway and network analysis separately. We identified 32 differentially expressed genes; IDH2, FEM1A, SSPB1 and their respective 30 co-expression genes; 68 differential proteins in KD. Significant proteins were categorized into 23 biological processes, 16 molecular functions, 16 cellular components, 15 function classes, 13 KD pathways and 1 network. IDH2, FEM1A, SSBP1, CALR, NDUFS2, IDH3A, GAPDH, TCA Cycle II (Eukaryotic) pathway and NADP repair pathway may play important roles in the pathogenesis of KD.

Place, publisher, year, edition, pages
London: Nature Publishing Group, 2018
Keywords
Keshan disease, cardiomyopathy, protoemics, gene array, integrative analysis
National Category
Cardiac and Cardiovascular Systems Cell and Molecular Biology
Research subject
Cardiology; Molecular Biology
Identifiers
urn:nbn:se:umu:diva-143903 (URN)10.1038/s41598-017-18599-x (DOI)000419668400005 ()29321553 (PubMedID)
Available from: 2018-01-14 Created: 2018-01-14 Last updated: 2018-06-09Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-6181-9904

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