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Zhao, G.-H., Yang, L., Lammi, M. & Guo, X. (2019). A preliminary analysis of microRNA profiles in the subchondral bone between Kashin-Beck disease and primary knee osteoarthritis. Clinical Rheumatology, 38(9), 2637-2645, Article ID 31062252.
Open this publication in new window or tab >>A preliminary analysis of microRNA profiles in the subchondral bone between Kashin-Beck disease and primary knee osteoarthritis
2019 (English)In: Clinical Rheumatology, ISSN 0770-3198, E-ISSN 1434-9949, Vol. 38, no 9, p. 2637-2645, article id 31062252Article in journal (Refereed) Published
Abstract [en]

INTRODUCTION: Kashin-Beck disease (KBD) is a chronic osteochondral disorder primarily associated with cartilage degeneration. The bone texture structure in KBD was also changed but it was not identical to primary knee osteoarthritis (OA). This study investigates the differences in microRNA (miRNA) profiles of subchondral bone collected from patients suffering from KBD in comparison with those with primary knee osteoarthritis (OA).

METHODS: Subchondral bone tissues were taken from four patients with KBD and four patients with primary knee OA undergoing total knee replacement. The miRNA array profiling was performed using an Affymetrix miRNA 4.0 Array, and then the target gene predictions and function annotations of the predicted targets were performed.

RESULTS: Our results showed that 124 miRNAs had lower expression levels in the subchondral bone sampled from KBD patients in comparison with OA patients. Gene ontology (GO) and KEGG pathway analyses of the predicted targets demonstrated numerous significantly enriched GO terms and signal pathways essential for bone development and integrity, such as metabolic processes, PI3K-Akt, and MAPK signaling pathways.

CONCLUSIONS: Our study confirms that a large set of miRNAs are differentially expressed in the subchondral bone of patients with KBD and OA and contributes new insights into potential pathological changes in the subchondral bone of KBD patients.

Keywords
Kashin-Beck disease, MicroRNAs, Osteoarthritis, Subchondral bone
National Category
Cell and Molecular Biology Orthopaedics Biochemistry and Molecular Biology Cell Biology
Research subject
Biochemistry; cell research; Molecular Biology; Orthopaedics
Identifiers
urn:nbn:se:umu:diva-158867 (URN)10.1007/s10067-019-04580-8 (DOI)31062252 (PubMedID)
Available from: 2019-05-13 Created: 2019-05-13 Last updated: 2019-10-09Bibliographically approved
Ning, Y., Wang, X., Lammi, M. & Guo, X. (2019). Changes in the NF-κB signaling pathway in juvenile and adult patients with Kashin-Beck disease. Experimental Cell Research, 379(2), 140-149
Open this publication in new window or tab >>Changes in the NF-κB signaling pathway in juvenile and adult patients with Kashin-Beck disease
2019 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 379, no 2, p. 140-149Article in journal (Refereed) Published
Abstract [en]

To investigate the pathogenesis of Kashin-Beck disease (KBD), we compared the common signaling pathways in peripheral blood mononuclear cells (PBMCs) obtained from healthy juvenile and adults and KBD patients, and also from osteoarthritis (OA) patients. The PBMCs from 12 KBD and 12 healthy juvenile, and those from 20 adult KBD patients and 12 healthy donors were separately collected among the people living in the KBD endemic area. The patients were distinguished according to the national diagnosis criteria. Total RNAs were extracted for the determination of gene expressions by microarray analysis. Ingenuity Pathways Analysis (IPA) was employed to identify the signaling pathways significantly affected by juveniles' and adults' KBD, and OA. The expressions of NFκB-p65, cIAP2 and RANKL in the articular cartilage from both juvenile and adults were detected by immunohistochemistry. NF-κB signaling, apoptosis signaling, death receptor signaling and IL-6 signaling pathways were revealed to be the common affected signaling pathways in the juvenile and adult KBD and the OA. BIRC3 and EGR1 were identified as two common differentially expressed genes. The percentages of positive staining of NFκB-p65, cIAP2 and RANKL were reduced in adult KBD patients but significantly increased in juvenile KBD patients. NF-κB, one of the common signaling pathways between adult and juvenile KBD, was less prominent in the adult KBD patients.

Place, publisher, year, edition, pages
Elsevier, 2019
Keywords
Common differentially expressed genes, Common signaling pathways, Immunohistochemistry, Kashin-Beck disease, Peripheral blood mononuclear cells
National Category
Cell and Molecular Biology Orthopaedics Cell Biology
Research subject
Biochemistry; cell research; Orthopaedics; rheumatology
Identifiers
urn:nbn:se:umu:diva-158869 (URN)10.1016/j.yexcr.2019.04.001 (DOI)000466827300002 ()30951708 (PubMedID)
Available from: 2019-05-13 Created: 2019-05-13 Last updated: 2019-06-13Bibliographically approved
Prittinen, J., Ylärinne, J., Piltti, J., Karhula, S. S., Rieppo, L., Ojanen, S. P., . . . Qu, C. (2019). Effect of centrifugal force on the development of articular neocartilage with bovine primary chondrocytes. Cell and Tissue Research, 375(3), 629-639
Open this publication in new window or tab >>Effect of centrifugal force on the development of articular neocartilage with bovine primary chondrocytes
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2019 (English)In: Cell and Tissue Research, ISSN 0302-766X, E-ISSN 1432-0878, Vol. 375, no 3, p. 629-639Article in journal (Refereed) Published
Abstract [en]

A lot has been invested into understanding how to assemble cartilage tissue in vitro and various designs have been developed to manufacture cartilage tissue with native-like biological properties. So far, no satisfactory design has been presented. Bovine primary chondrocytes are used to self-assemble scaffold-free constructs to investigate whether mechanical loading by centrifugal force would be useful in manufacturing cartilage tissue in vitro. Six million chondrocytes were laid on top of defatted bone disks placed inside an agarose well in 50-ml culture tubes. The constructs were centrifuged once or three times per day for 15 min at a centrifugal force of 771×g for up to 4 weeks. Control samples were cultured under the same conditions without exposure to centrifugation. The samples were analysed by (immuno)histochemistry, Fourier transform infrared imaging, micro-computed tomography, biochemical and gene expression analyses. Biomechanical testing was also performed. The centrifuged tissues had a more even surface covering a larger area of the bone disk. Fourier transform infrared imaging analysis indicated a higher concentration of collagen in the top and bottom edges in some of the centrifuged samples. Glycosaminoglycan contents increased along the culture, while collagen content remained at a rather constant level. Aggrecan and procollagen α1(II) gene expression levels had no significant differences, while procollagen α2(I) levels were increased significantly. Biomechanical analyses did not reveal remarkable changes. The centrifugation regimes lead to more uniform tissue constructs, whereas improved biological properties of the native tissue could not be obtained by centrifugation.

Place, publisher, year, edition, pages
New York: Springer, 2019
Keywords
Cartilage tissue engineering, Centrifugal force, Osteoarthritis, Primary chondrocyte, Tissue assembly
National Category
Cell and Molecular Biology Orthopaedics Biochemistry and Molecular Biology Cell Biology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Biochemistry; biomechanics; cell research; Orthopaedics
Identifiers
urn:nbn:se:umu:diva-152816 (URN)10.1007/s00441-018-2938-3 (DOI)000460535300006 ()30349935 (PubMedID)
Available from: 2018-11-08 Created: 2018-11-08 Last updated: 2019-04-08Bibliographically approved
Zhu, Y.-H., Wang, X.-F., Yang, G., Wei, J., Tan, W.-H., Wang, L.-X., . . . Xu, J.-H. (2019). Efficacy of long-term selenium supplementation in the treatment of chronic Keshan disease with congestive heart failure. Current medical science, 39(2), 237-242
Open this publication in new window or tab >>Efficacy of long-term selenium supplementation in the treatment of chronic Keshan disease with congestive heart failure
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2019 (English)In: Current medical science, ISSN 2096-5230, Vol. 39, no 2, p. 237-242Article in journal (Refereed) Published
Abstract [en]

Few effective treatments for chronic Keshan disease have been available till now. The efficacy of long-term selenium supplementation in the treatment of chronic Keshan disease with congestive heart failure is inconclusive. This study aimed to determine whether selenium supplementation is associated with a decreased risk of cardiac death in chronic Keshan disease with congestive heart failure by ten years of follow-up. A retrospective long-term follow-up analysis was performed on a monitored cohort consisting of 302 chronic Keshan disease patients with a mean age of 40.8±11.4 years. Of the 302 chronic Keshan disease patients, 170 (56.3%) were given selenium supplementation until the end point of follow-up. Cox proportional hazards regression models were used to identify the independent predictors of cardiac events. Our results showed that during the follow-up, there were 101 deaths of patients with chronic Keshan disease in the selenium supplementation group (101/170, 59.4%) and 98 in non-selenium supplementation group (98/132, 74.2%). Multivariate analyses suggested that selenium supplementation was associated with a decreased risk of cardiac death (HR 0.39, 95% CI 0.28-0.53) after adjustment for baseline age, sex, cigarette smoking, family history of Keshan disease, body mass index (BMI), heart rate, electrocardiogram (ECG) abnormalities, blood pressure, initial cardiothoracic ratio, left ventricular ejection fractions (LVEF) and whole-blood selenium concentration. Our ten-year follow-up analysis indicated that selenium supplementation, specifically combined with the use of angiotensin-converting enzyme inhibitor and beta blocker therapy, improved the survival of patients with chronic Keshan disease with congestive heart failure. BMI, selenium deficiency, male, combined ECG abnormalities, LVEF, and fast heart rate increased the risk of cardiac events.

Place, publisher, year, edition, pages
Springer, 2019
Keywords
Keshan disease, follow-up, selenium supplementation
National Category
Cell and Molecular Biology Cardiac and Cardiovascular Systems Nutrition and Dietetics
Research subject
Biochemistry; Cardiology; Nutrition
Identifiers
urn:nbn:se:umu:diva-158868 (URN)10.1007/s11596-019-2025-3 (DOI)000465447300010 ()31016516 (PubMedID)
Available from: 2019-05-13 Created: 2019-05-13 Last updated: 2019-05-20Bibliographically approved
Wang, M., Xue, S., Fang, Q., Zhang, M., He, Y., Zhang, Y., . . . Chen, J. (2019). Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency. Glycoconjugate Journal
Open this publication in new window or tab >>Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency
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2019 (English)In: Glycoconjugate Journal, ISSN 0282-0080, E-ISSN 1573-4986Article in journal (Refereed) Epub ahead of print
Abstract [en]

Kashin-Beck disease (KBD) is an endemic degenerative osteoarthropathy of uncertain etiology. Our study sought to identify a correlation between small proteoglycans decorin and biglycan expression and Kashin-Beck Disease. Immunohistochemistry was used to assess the decorin and biglycan levels in cartilage specimens from both child KBD patients, and rats fed with T-2 toxin under a selenium-deficient condition. Real-time PCR and Western blot were used to assess mRNA and protein levels of decorin and biglycan in rat cartilages, as well as in C28/I2 chondrocytes stimulated by T-2 toxin and selenium in vitro. The result showed that decorin was reduced in all zones of KBD articular cartilage, while the expression of biglycan was prominently increased in KBD cartilage samples. Increased expression of biglycan and reduced expression of decorin were observed at mRNA and protein levels in the cartilage of rats fed with T-2 toxin and selenium- deficiency plus T-2 toxin diet, when compared with the normal diet group. Moreover, In vitro stimulation of C28/I2 cells with T-2 toxin resulted in an upregulation of biglycan and downregulation of decorin, T-2 toxin induction of biglycan and decorin levels were partly rescued by selenium supplement. This study highlights the focal nature of the degenerative changes that occur in KBD cartilage and may suggest that the altered expression pattern of decorin and biglycan have an important role in the onset and pathogenesis of KBD.

Place, publisher, year, edition, pages
Springer, 2019
Keywords
Biglycan, Decorin, Extracellular matrix, Kashin-Beck disease, Selenium, T-2 toxin
National Category
Cell and Molecular Biology Orthopaedics Pharmacology and Toxicology
Research subject
cell research; Biochemistry; Orthopaedics; Toxicology
Identifiers
urn:nbn:se:umu:diva-163127 (URN)10.1007/s10719-019-09889-9 (DOI)31478096 (PubMedID)
Available from: 2019-09-09 Created: 2019-09-09 Last updated: 2019-09-10
Lin, X., Shao, W., Yu, F., Xing, K., Liu, H., Zhang, F., . . . Guo, X. (2019). Individual and combined toxicity of T-2 toxin and deoxynivalenol on human C-28/I2 and rat primary chondrocytes. Journal of Applied Toxicology, 39(2), 343-353, Article ID 30251759.
Open this publication in new window or tab >>Individual and combined toxicity of T-2 toxin and deoxynivalenol on human C-28/I2 and rat primary chondrocytes
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2019 (English)In: Journal of Applied Toxicology, ISSN 0260-437X, E-ISSN 1099-1263, Vol. 39, no 2, p. 343-353, article id 30251759Article in journal (Refereed) Published
Abstract [en]

Deoxynivalenol (DON) and T-2 toxin are prevalent mycotoxin contaminants in the food and feed stuffs worldwide, with non-negligible co-contamination and co-exposure conditions. Meanwhile, they are considerable risk factors for Kashin-Beck disease, a chronic endemic osteochondropathy. The aim of this study was to investigate the individual and combined cytotoxicity of DON and T-2 toxin on proliferating human C-28/I2 and newborn rat primary costal chondrocytes by MTT assay. Four molar concentration combination ratios of DON and T-2 toxin were used, 1:1 for R1 mixture, 10:1 for R10, 100:1 for R100 and 1000:1 for R1000. The toxicological interactions were quantified by the MixLow method. DON, T-2 toxin, and their mixtures all showed a clear dose-dependent toxicity for chondrocytes. The cytotoxicity of T-2 toxin was 285-fold higher than DON was in human chondrocytes, and 22-fold higher in the rat chondrocytes. The combination of DON and T-2 toxin was significantly synergistic at middle and high level concentrations of R10 mixtures in rat chondrocytes, but significantly antagonistic at the low concentrations of R100 mixtures in both cells and at the middle concentrations of R1000 mixtures in rat chondrocytes. These results indicated that the combined toxicity was influenced by the cell sensitivity for toxins, the difference between the combination ratio and equitoxic ratio, the concentrations and other factors.

Place, publisher, year, edition, pages
John Wiley & Sons, 2019
Keywords
T-2 toxin, antagonism, chondrocyte, combined toxicity, deoxynivalenol, synergism
National Category
Pharmacology and Toxicology Cell Biology Cell and Molecular Biology
Research subject
cellforskning; Toxicology
Identifiers
urn:nbn:se:umu:diva-152125 (URN)10.1002/jat.3725 (DOI)000456205000015 ()30251759 (PubMedID)
Available from: 2018-09-27 Created: 2018-09-27 Last updated: 2019-02-26Bibliographically approved
Wang, Y., Wu, C., Yang, Y., Ren, Z., Lammi, M. & Guo, X. (2019). Preliminary exploration of hsa_circ_0032131 levels in peripheral blood as a potential diagnostic biomarker of osteoarthritis. Genetic Testing and Molecular Biomarkers, 23(10), 717-721
Open this publication in new window or tab >>Preliminary exploration of hsa_circ_0032131 levels in peripheral blood as a potential diagnostic biomarker of osteoarthritis
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2019 (English)In: Genetic Testing and Molecular Biomarkers, ISSN 1945-0265, E-ISSN 1945-0257, Vol. 23, no 10, p. 717-721Article in journal (Refereed) Published
Abstract [en]

Background: Osteoarthritis (OA) is a common chronic degenerative joint disease characterized by articular cartilage degeneration and synovitis. CircRNAs are increasingly being recognized as functional endogenous RNAs with a stable structure and high tissue specificity. Recent studies have shown that some circRNAs may be involved in the initiation and progression of OA and that there is differential expression of circRNAs in chondrocytes in vitro isolated from patients with OA.

Purposes: In this study, we aimed to determine if circRNA levels in the peripheral blood of Chinese Han patients with OA would be diagnostic based on the previous in vitro studies.

Methods: We collected peripheral blood samples from 25 patients suffering from OA and 25 healthy controls and measured hsa_circ_0032131_CBC1 RNA levels through quantitative RT-PCR (qRT-PCR). The statistical basis for evaluating the diagnostic value was to calculate the area under the receiver operator characteristic (ROC) curve.

Results: The results of the qRT-PCR for hsa_circ_0032131_CBC1 were consistent with those of the microarray analysis. The ROC curve shows that hsa_circ_0032131 holds diagnostic value for OA (0.8455, p < 0.01).

Conclusions: Our research indicates that differentially expressed circRNAs may be involved in the development of OA and could be used diagnostically.

Place, publisher, year, edition, pages
Mary Ann Liebert, 2019
Keywords
circRNA, diagnostic biomarker, osteoarthritis, peripheral blood
National Category
Cell and Molecular Biology Rheumatology and Autoimmunity Orthopaedics Biomedical Laboratory Science/Technology
Research subject
Molecular Biology; Orthopaedics; rheumatology; Medical Biochemistry
Identifiers
urn:nbn:se:umu:diva-163634 (URN)10.1089/gtmb.2019.0036 (DOI)000486800600001 ()31526191 (PubMedID)
Available from: 2019-10-01 Created: 2019-10-01 Last updated: 2019-11-11Bibliographically approved
Wang, Y., Wu, C., Zhang, F., Zhang, Y., Ren, Z., Lammi, M. & Guo, X. (2019). Screening for differentially expressed circular RNAs in the cartilage of osteoarthritis patients for their diagnostic value. Genetic Testing and Molecular Biomarkers, 23(10), 706-716, Article ID 31502887.
Open this publication in new window or tab >>Screening for differentially expressed circular RNAs in the cartilage of osteoarthritis patients for their diagnostic value
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2019 (English)In: Genetic Testing and Molecular Biomarkers, ISSN 1945-0265, E-ISSN 1945-0257, Vol. 23, no 10, p. 706-716, article id 31502887Article in journal (Refereed) Published
Abstract [en]

Background: Osteoarthritis (OA) is the most prevailing osteoarticular disease, which typically involves chronic cartilage degeneration and synovitis. The latest research shows that circular RNAs (circRNAs) play a role in the development of a variety of diseases, including osteoarthrosis.

Purposes: The aim of this study was to explore the expression of circRNAs in OA chondrocytes and predict biomarkers for diagnosis.

Materials and Methods: The circRNA expression profile was analyzed through use of the Gene Spring software V13.0; differentially expressed circRNAs were screened over a process of OA. We validated the microarray data by quantitative real-time polymerase chain reaction analyses of OA chondrocytes and chondrocytes from normal controls. TargetScan software and miRanda software were used to predict network analysis of circRNA-miRNA interactions in cartilages. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) analyses were applied to predict the functions of differentially expressed circRNAs.

Results: Overall 1380 circRNAs were differentially expressed in OA chondrocytes in contrast to the normal articular cartilages (fold-change ≥2, p ≤ 0.05), consisting of 215 upregulated and 1165 downregulated circRNAs. After analyzing the differentially expressed circRNA genes, the top 20 enriched GO entries and the KEGG pathways were annotated. Furthermore, hsa_circrna_0032131 was identified for further analysis. The circRNA-miRNA network was constructed to represent the 10 most likely target genes associated with the validated circRNA.

Conclusions: Our research suggests that some of the differentially expressed circRNAs in OA chondrocytes compared to normal chondrocytes are etiologically associated with the pathological process of OA. It was found that hsa_circRNA_0032131 likely participates in the initiation and progression of OA and has potential as a diagnostic marker.

Clinical Relevance: To analyze the difference of circRNA expression profiles between OA and normal controls and explore biomarkers for diagnosis.

Place, publisher, year, edition, pages
Mary Ann Liebert, 2019
Keywords
circRNAs, expression profile, functional analysis, osteoarthritis
National Category
Cell and Molecular Biology Orthopaedics Rheumatology and Autoimmunity Biomedical Laboratory Science/Technology
Research subject
Molecular Biology; Clinical Chemistry; Orthopaedics; rheumatology; Medical Biochemistry
Identifiers
urn:nbn:se:umu:diva-163635 (URN)10.1089/gtmb.2019.0108 (DOI)31502887 (PubMedID)
Available from: 2019-10-01 Created: 2019-10-01 Last updated: 2019-10-22
Liu, H., Yu, F., Shao, W., Ding, D., Yu, Z., Chen, F., . . . Guo, X. (2018). Associations between selenium content in hair and Kashin-Beck Disease/Keshan Disease in children in Northwestern China: a prospective cohort study. Biological Trace Element Research, 184(1), 16-23, Article ID 28983831.
Open this publication in new window or tab >>Associations between selenium content in hair and Kashin-Beck Disease/Keshan Disease in children in Northwestern China: a prospective cohort study
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2018 (English)In: Biological Trace Element Research, ISSN 0163-4984, E-ISSN 1559-0720, Vol. 184, no 1, p. 16-23, article id 28983831Article in journal (Refereed) Published
Abstract [en]

The objective of this study was to investigate the relationship between selenium content in hair and the incidence of Kashin-Beck disease (KBD) and Keshan disease (KD) in China. A prospective cohort study was conducted among children aged 5-12 years with different levels of low-selenium (group 1, Se ≤ 110 ng/g; group 2, 110 < Se ≤ 150 ng/g; and group 3, 150 < Se ≤ 200 ng/g) or selenium-supplemented (group 4, Se > 200 ng/g) exposure. A person-years approach was used to calculate the incidence and rate of positive clinical signs. Relative risk (RR), attributable risk, and etiologic fraction were used to determine the strength of association between selenium and disease incidence. Seven new KBD cases were diagnosed during 3-year follow-up. Positive clinical signs of KBD were found in 17.78 (95% confidence interval [CI] 14.27-21.29) cases per 100 person-years in group 1, 13.28 (9.82-16.74) in group 2, 12.95 (9.34-16.56) in group 3, and 8.18 (5.50-10.85) in group 4. Compared with group 4, the RR (95% CI) of groups 1, 2, and 3 were 2.17 (1.48-3.19), 1.62 (1.07-2.47), and 1.58 (1.03-2.43), respectively. Positive clinical signs of KD were 25.90 (18.62-33.18) cases per 100 person-years in group 1, 5.66 (1.26-10.06) in group 2, 4.60 (0.20-9.00) in group 3, and 14.62 (8.54-20.69) in group 4. Compared with group 4, the RR (95% CI) were 1.77 (1.07-2.93), 0.39 (0.16-0.93), and 0.31 (0.11-0.89), respectively. In children, the onset of KBD was negatively correlated with selenium content within a certain range. However, there may be a U-shaped association between selenium content and KD in children.

Place, publisher, year, edition, pages
Springer, 2018
Keywords
Cohort study, Kashin-Beck disease, Keshan disease, Selenium
National Category
Pediatrics Public Health, Global Health, Social Medicine and Epidemiology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Pediatrics
Identifiers
urn:nbn:se:umu:diva-140367 (URN)10.1007/s12011-017-1169-x (DOI)000434723900003 ()28983831 (PubMedID)
Available from: 2017-10-08 Created: 2017-10-08 Last updated: 2018-08-27Bibliographically approved
Lammi, M., Piltti, J., Prittinen, J. & Qu, C. (2018). Challenges in fabrication of tissue-engineered cartilage with correct cellular colonization and extracellular matrix assembly. International Journal of Molecular Sciences, 19(9), Article ID 2700.
Open this publication in new window or tab >>Challenges in fabrication of tissue-engineered cartilage with correct cellular colonization and extracellular matrix assembly
2018 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 19, no 9, article id 2700Article, review/survey (Refereed) Published
Abstract [en]

A correct articular cartilage ultrastructure regarding its structural components and cellularity is important for appropriate performance of tissue-engineered articular cartilage. Various scaffold-based, as well as scaffold-free, culture models have been under development to manufacture functional cartilage tissue. Even decellularized tissues have been considered as a potential choice for cellular seeding and tissue fabrication. Pore size, interconnectivity, and functionalization of the scaffold architecture can be varied. Increased mechanical function requires a dense scaffold, which also easily restricts cellular access within the scaffold at seeding. High pore size enhances nutrient transport, while small pore size improves cellular interactions and scaffold resorption. In scaffold-free cultures, the cells assemble the tissue completely by themselves; in optimized cultures, they should be able to fabricate native-like tissue. Decellularized cartilage has a native ultrastructure, although it is a challenge to obtain proper cellular colonization during cell seeding. Bioprinting can, in principle, provide the tissue with correct cellularity and extracellular matrix content, although it is still an open question as to how the correct molecular interaction and structure of extracellular matrix could be achieved. These are challenges facing the ongoing efforts to manufacture optimal articular cartilage.

Place, publisher, year, edition, pages
MDPI, 2018
Keywords
articular cartilage, cartilage architecture, cell colonization, extracellular matrix, tissue engineering
National Category
Cell and Molecular Biology Orthopaedics Biochemistry and Molecular Biology Cell Biology
Research subject
Biochemistry; cellforskning; Orthopaedics
Identifiers
urn:nbn:se:umu:diva-151888 (URN)10.3390/ijms19092700 (DOI)000449988100236 ()30208585 (PubMedID)2-s2.0-85053359968 (Scopus ID)
Available from: 2018-09-16 Created: 2018-09-16 Last updated: 2018-12-18Bibliographically approved
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Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-6181-9904

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