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Publications (10 of 56) Show all publications
Demaret, L., Gorzsás, A., Altunayar-Unsalan, C., Unsalan, O. & Yeşilbaş, M. (2024). Biosignature detection on ice using raman spectroscopy: a case study for hydromagnesites from Lake Salda, Turkiye. In: LPSC 2024: program with links to abstracts. Paper presented at LPSC 2024, the 55th Lunar and Planetary Science Conference, The Woodlands, USA/virtual, March 11-15, 2024. Lunar and Planetary Institute, Article ID 2497.
Open this publication in new window or tab >>Biosignature detection on ice using raman spectroscopy: a case study for hydromagnesites from Lake Salda, Turkiye
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2024 (English)In: LPSC 2024: program with links to abstracts, Lunar and Planetary Institute , 2024, article id 2497Conference paper, Oral presentation with published abstract (Other academic)
Abstract [en]

Detection of organic biosignatures by (cryo-)Raman spectroscopy in hydromagnesite-rich samples from Lake Salda, an analogue site for the Mg-carbonate deposits in Jezero Crater.

Place, publisher, year, edition, pages
Lunar and Planetary Institute, 2024
National Category
Inorganic Chemistry Physical Chemistry Geochemistry Geosciences, Multidisciplinary
Research subject
Analytical Chemistry; Physical Chemistry
Identifiers
urn:nbn:se:umu:diva-233190 (URN)
Conference
LPSC 2024, the 55th Lunar and Planetary Science Conference, The Woodlands, USA/virtual, March 11-15, 2024
Funder
Swedish Research Council, VR-2021-05859The Kempe Foundations, JCK22-0065
Available from: 2024-12-25 Created: 2024-12-25 Last updated: 2025-01-03Bibliographically approved
Niemi, C., Takahashi, J., Gorzsás, A. & Gentili, F. G. (2024). Quantitative and qualitative saccharide analysis of North Atlantic brown seaweed by gas chromatography/mass spectrometry and infrared spectroscopy. International Journal of Biological Macromolecules, 254, Article ID 127870.
Open this publication in new window or tab >>Quantitative and qualitative saccharide analysis of North Atlantic brown seaweed by gas chromatography/mass spectrometry and infrared spectroscopy
2024 (English)In: International Journal of Biological Macromolecules, ISSN 0141-8130, E-ISSN 1879-0003, Vol. 254, article id 127870Article in journal (Refereed) Published
Abstract [en]

Brown seaweeds contain a variety of saccharides which have potential industrial uses. The most abundant polysaccharide in brown seaweed is typically alginate, consisting of mannuronic (M) and guluronic acid (G). The ratio of these residues fundamentally determines the physicochemical properties of alginate. In the present study, gas chromatography/mass spectrometry (GC/MS) was used to give a detailed breakdown of the monosaccharide species in North Atlantic brown seaweeds. The anthrone method was used for determination of crystalline cellulose. The experimental data was used to calibrate multivariate prediction models for estimation of total carbohydrates, crystalline cellulose, total alginate and alginate M/G ratio directly in dried, brown seaweed using three types of infrared spectroscopy, using relative error (RE) as a measure of predictive accuracy. Diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) performed well for the estimation of total alginate (RE = 0.12, R2 = 0.82), and attenuated total reflectance (ATR) showed good prediction of M/G ratio (RE = 0.14, R2 = 0.86). Both DRIFTS, ATR and near infrared (NIR) were unable to predict crystalline cellulose and only DRIFTS performed better in determining total carbohydrates. Multivariate spectral analysis is a promising method for easy and rapid characterization of alginate and M/G ratio in seaweed.

Place, publisher, year, edition, pages
Elsevier, 2024
Keywords
Alginate, FTIR, GC/MS, MG ratio, North Atlantic brown seaweed
National Category
Bioenergy Organic Chemistry
Identifiers
urn:nbn:se:umu:diva-219754 (URN)10.1016/j.ijbiomac.2023.127870 (DOI)001120565300001 ()37967607 (PubMedID)2-s2.0-85181751462 (Scopus ID)
Available from: 2024-01-19 Created: 2024-01-19 Last updated: 2025-02-17Bibliographically approved
Wojciechowska, A., Janczak, J., Jarzembska, K. N., Rojek, T., Gorzsás, A., Kersen, Ü., . . . Jezierska, J. (2023). Chelidamic acid tautomers in copper(ii) compounds: one-pot synthesis, crystal structure, spectroscopic and dft studies. Polyhedron, 230, Article ID 116210.
Open this publication in new window or tab >>Chelidamic acid tautomers in copper(ii) compounds: one-pot synthesis, crystal structure, spectroscopic and dft studies
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2023 (English)In: Polyhedron, ISSN 0277-5387, E-ISSN 1873-3719, Vol. 230, article id 116210Article in journal (Refereed) Published
Abstract [en]

Two copper(II) chelidamate compounds with 2,2'-bipyridine were obtained as crystals [Cu(Hcda)(bpy)(H2O)]∙2H2O (1) and [Cu(H2cda)(bpy)(H2O)](H2cda) (2) (H3L = H3cda = chel = chelidamic acid, bpy = 2,2′-bipyridine) through one-pot synthesis. In 1, the tridentate Hcda2− ligand, an enol tautomer of the 4-hydroxypyridine type, and a bpy molecule coordinate the Cu(II). In 2, the same tautomer of the tridentate H2cda− ligand (with one deprotonated carboxylic acid), along with bpy, forms a cationic [Cu(H2cda)(bpy)(H2O)]+ complex. It is charge-balanced by an H2chel− anion, featuring the rare keto tautomer of the 4-pyridone type. The alternately protonated and deprotonated carboxylic groups of both tautomers are involved in hydrogen bonds leading to 1D chains. The adjacent chains are connected to form 2D chain by hydrogen bonds between two different tautomers of chel. As confirmed by the DFT calculations, the crystal structures of 1 and 2 are mainly stabilised by hydrogen bonds and electrostatic interactions, the latter being especially noticeable in the [Cu(H2cda)(bpy)(H2O)]+ cationic complex (2). Additionally, π⋯π stack is formed between aromatic ring fragments. The presence of each particular tautomer in the crystal structures of 1 and 2 can be explained by the relative energies of the respective structural moieties in the aqueous environment, combined with coordination and intermolecular interaction preferences.

Place, publisher, year, edition, pages
Elsevier, 2023
Keywords
Copper(II) complexes, Crystal structures, Spectroscopy (FT-IR, Raman, NIR-Vis-UV, EPR), Tautomers of chelidamic acid, Theoretical calculations
National Category
Physical Chemistry Inorganic Chemistry
Identifiers
urn:nbn:se:umu:diva-201639 (URN)10.1016/j.poly.2022.116210 (DOI)000903908700004 ()2-s2.0-85143124963 (Scopus ID)
Available from: 2022-12-13 Created: 2022-12-13 Last updated: 2023-09-05Bibliographically approved
Miranda, D. A., Marín, K., Sundman, O., Hedenström, M., Quillaguaman, J., Gorzsás, A., . . . Martin, C. (2023). Production and characterization of poly(3-hydroxybutyrate) from Halomonas boliviensis LC1 cultivated in hydrolysates of quinoa stalks. Fermentation, 9(6), Article ID 556.
Open this publication in new window or tab >>Production and characterization of poly(3-hydroxybutyrate) from Halomonas boliviensis LC1 cultivated in hydrolysates of quinoa stalks
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2023 (English)In: Fermentation, E-ISSN 2311-5637, Vol. 9, no 6, article id 556Article in journal (Refereed) Published
Abstract [en]

The global production of fossil-based plastics has reached critical levels, and their substitution with bio-based polymers is an urgent requirement. Poly(3-hydroxybutyrate) (PHB) is a biopolymer that can be produced via microbial cultivation, but efficient microorganisms and low-cost substrates are required. Halomonas boliviensis LC1, a moderately halophilic bacterium, is an effective PHB producer, and hydrolysates of the residual stalks of quinoa (Chenopodium quinoa Willd.) can be considered a cheap source of sugars for microbial fermentation processes in quinoa-producing countries. In this study, H. boliviensis LC1 was adapted to a cellulosic hydrolysate of quinoa stalks obtained via acid-catalyzed hydrothermal pretreatment and enzymatic saccharification. The adapted strain was cultivated in hydrolysates and synthetic media, each of them with two different initial concentrations of glucose. Cell growth, glucose consumption, and PHB formation during cultivation were assessed. The cultivation results showed an initial lag in microbial growth and glucose consumption in the quinoa hydrolysates compared to cultivation in synthetic medium, but after 33 h, the values were comparable for all media. Cultivation in hydrolysates with an initial glucose concentration of 15 g/L resulted in a higher glucose consumption rate (0.15 g/(L h) vs. 0.14 g/(L h)) and volumetric productivity of PHB (14.02 mg/(L h) vs. 10.89 mg/(L h)) than cultivation in hydrolysates with 20 g/L as the initial glucose concentration. During most of the cultivation time, the PHB yield on initial glucose was higher for cultivation in synthetic medium than in hydrolysates. The produced PHBs were characterized using advanced analytical techniques, such as high-performance size-exclusion chromatography (HPSEC), Fourier transform infrared (FTIR) spectroscopy, 1H nuclear magnetic resonance (NMR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). HPSEC revealed that the molecular weight of PHB produced in the cellulosic hydrolysate was lower than that of PHB produced in synthetic medium. TGA showed higher thermal stability for PHB produced in synthetic medium than for that produced in the hydrolysate. The results of the other characterization techniques displayed comparable features for both PHB samples. The presented results show the feasibility of producing PHB from quinoa stalks with H. boliviensis.

Place, publisher, year, edition, pages
MDPI, 2023
Keywords
adaptation, agricultural residues, biopolymers, Halomonas boliviensis, halophilic bacteria, lignocellulosic materials, polyhydroxybutyrate, quinoa
National Category
Biochemistry Molecular Biology
Identifiers
urn:nbn:se:umu:diva-212048 (URN)10.3390/fermentation9060556 (DOI)001017168000001 ()2-s2.0-85163753314 (Scopus ID)
Funder
Swedish Research Council, 2016-05822Bio4Energy
Available from: 2023-07-18 Created: 2023-07-18 Last updated: 2025-02-20Bibliographically approved
Niemi, C., Mortensen, A. M., Rautenberger, R., Matsson, S., Gorzsás, A. & Gentili, F. G. (2023). Rapid and accurate determination of protein content in North Atlantic seaweed by NIR and FTIR spectroscopies. Food Chemistry, 404, Article ID 134700.
Open this publication in new window or tab >>Rapid and accurate determination of protein content in North Atlantic seaweed by NIR and FTIR spectroscopies
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2023 (English)In: Food Chemistry, ISSN 0308-8146, E-ISSN 1873-7072, Vol. 404, article id 134700Article in journal (Refereed) Published
Abstract [en]

Seaweed is considered a potentially sustainable source of protein for human consumption, and rapid, accurate methods for determining seaweed protein contents are needed. Seaweeds contain substances which interfere with common protein estimation methods however. The present study compares the Lowry and BCA protein assays and protein determination by N-ratios to more novel spectroscopic methods. Linear regression of the height or the integrated area under the Amide II band of diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) was used to predict seaweed protein with good prediction performance. Partial least squares regression (PLSR) was performed on both DRIFTS and near-infrared (NIR) spectra, with even higher prediction accuracy. Spectroscopy performed similar to or better than the calculated N-ratio of 4.14 for protein prediction. These spectral prediction methods require minimal sample preparation and chemical use, and are easy to perform, making them environmentally sustainable and economically viable for rapid estimation of seaweed protein.

Place, publisher, year, edition, pages
Elsevier, 2023
Keywords
FTIR, NIR, Protein, Seaweed, Spectroscopy
National Category
Food Science
Identifiers
urn:nbn:se:umu:diva-213592 (URN)10.1016/j.foodchem.2022.134700 (DOI)000930583100001 ()36279781 (PubMedID)2-s2.0-85140336412 (Scopus ID)
Available from: 2023-08-29 Created: 2023-08-29 Last updated: 2023-08-29Bibliographically approved
Ranade, S. S., Seipel, G., Gorzsás, A. & García-Gil, M. R. (2022). Adaptive strategies of Scots pine under shade: Increase in lignin synthesis and ecotypic variation in defense-related gene expression. Physiologia Plantarum, 174(5), Article ID e13792.
Open this publication in new window or tab >>Adaptive strategies of Scots pine under shade: Increase in lignin synthesis and ecotypic variation in defense-related gene expression
2022 (English)In: Physiologia Plantarum, ISSN 0031-9317, E-ISSN 1399-3054, Vol. 174, no 5, article id e13792Article in journal (Refereed) Published
Abstract [en]

Shade is a stressful condition for plants characterized by low Red:Far-Red (R:FR) ratio. The northern latitudes in Sweden daily receive more hours of FR-enriched light (twilight) or shade-like conditions compared to southern forests during the growing season. Scots pine (Pinus sylvestris L.) is a shade-intolerant species. Yet, it is well adapted to this latitudinal variation in light, which is evident by a northward increase in FR requirement to maintain growth. Shade adversely affects plant growth; it makes the plant weak and, therefore, susceptible to pathogen attack. Lignin is involved in plant protection against pathogen invasion mainly by forming a physical barrier. We studied lignin synthesis and expression of defense-related genes (growth-defense trade-offs) under a low R:FR (shade) ratio in Scots pine. A higher number of immunity/defense-related genes were up-regulated in response to shade in northern populations compared to southern ones, which can be viewed as a local adaptation to light quality for optimal growth and survival. Light quality regulates lignin metabolism; light stimulates lignin synthesis, while shade causes a decrease in lignin synthesis in most angiosperms. In contrast, Scots pine shows an increase in lignin synthesis supported by the higher expression of a few key genes in the lignin biosynthetic pathway, a novel finding reported by our study. These findings can be applied to future breeding strategies in forestry to produce disease-resilient trees.

Place, publisher, year, edition, pages
John Wiley & Sons, 2022
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-200830 (URN)10.1111/ppl.13792 (DOI)000868840000001 ()36177740 (PubMedID)2-s2.0-85140584680 (Scopus ID)
Funder
The Kempe Foundations, JCK-1311The Royal Swedish Academy of Agriculture and Forestry (KSLA), KSLA-H14-0150-ADASwedish Research CouncilVinnovaSwedish Research Council Formas, FA-2021/0038
Available from: 2022-11-11 Created: 2022-11-11 Last updated: 2023-03-24Bibliographically approved
Marinovíc, M., Di Falco, M., Aguilar Pontes, M. V., Gorzsás, A., Tsang, A., de Vries, R. P., . . . Hildén, K. (2022). Comparative Analysis of Enzyme Production Patterns of Lignocellulose Degradation of Two White Rot Fungi: Obba rivulosa and Gelatoporia subvermispora. Biomolecules, 12(8), Article ID 1017.
Open this publication in new window or tab >>Comparative Analysis of Enzyme Production Patterns of Lignocellulose Degradation of Two White Rot Fungi: Obba rivulosa and Gelatoporia subvermispora
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2022 (English)In: Biomolecules, E-ISSN 2218-273X, Vol. 12, no 8, article id 1017Article in journal (Refereed) Published
Abstract [en]

The unique ability of basidiomycete white rot fungi to degrade all components of plant cell walls makes them indispensable organisms in the global carbon cycle. In this study, we analyzed the proteomes of two closely related white rot fungi, Obba rivulosa and Gelatoporia subvermispora, during eight-week cultivation on solid spruce wood. Plant cell wall degrading carbohydrate-active enzymes (CAZymes) represented approximately 5% of the total proteins in both species. A core set of orthologous plant cell wall degrading CAZymes was shared between these species on spruce suggesting a conserved plant biomass degradation approach in this clade of basidiomycete fungi. However, differences in time-dependent production of plant cell wall degrading enzymes may be due to differences among initial growth rates of these species on solid spruce wood. The obtained results provide insight into specific enzymes and enzyme sets that are produced during the degradation of solid spruce wood in these fungi. These findings expand the knowledge on enzyme production in nature-mimicking conditions and may contribute to the exploitation of white rot fungi and their enzymes for biotechnological applications.

Place, publisher, year, edition, pages
MDPI, 2022
Keywords
CAZymes, Gelatoporia subvermispora, LC-MS/MS, lignin biodegradation, Obba rivulosa, proteome, white rot fungi
National Category
Microbiology
Identifiers
urn:nbn:se:umu:diva-199374 (URN)10.3390/biom12081017 (DOI)000846147100001 ()35892327 (PubMedID)2-s2.0-85137323191 (Scopus ID)
Funder
EU, FP7, Seventh Framework Programme, 607044
Note

Special Issue: Regulation and Mechanisms of Plant Biomass Degrading Enzymes from Fungi

Available from: 2022-09-26 Created: 2022-09-26 Last updated: 2022-09-26Bibliographically approved
Ranade, S. S., Seipel, G., Gorzsás, A. & García-Gil, M. R. (2022). Enhanced lignin synthesis and ecotypic variation in defense-related gene expression in response to shade in Norway spruce. Plant, Cell and Environment, 45(9), 2671-2681
Open this publication in new window or tab >>Enhanced lignin synthesis and ecotypic variation in defense-related gene expression in response to shade in Norway spruce
2022 (English)In: Plant, Cell and Environment, ISSN 0140-7791, E-ISSN 1365-3040, Vol. 45, no 9, p. 2671-2681Article in journal (Refereed) Published
Abstract [en]

During the growth season, northern forests in Sweden daily receive more hours of far-red (FR)-enriched light or twilight (shade) as compared to southern forests. Norway spruce (shade-tolerant) are adapted to latitudinal variation in twilight characterized by a northward increase in FR requirement to maintain growth. Shade is a stressful condition that affects plant growth and increases plant's susceptibility to pathogen attack. Lignin plays a central role in plant defense and its metabolism is regulated by light wavelength composition (light quality). In the current work, we studied regulation of lignin synthesis and defense-related genes (growth-defense trade-offs) in response to shade in Norway spruce. In most angiosperms, light promotes lignin synthesis, whereas shade decreases lignin production leading to weaker stem, which may make plants more disease susceptible. In contrast, enhanced lignin synthesis was detected in response to shade in Norway spruce. We detected a higher number of immunity/defense-related genes up-regulated in northern populations as compared to south ones in response to shade. Enhanced lignin synthesis coupled with higher defense-related gene expression can be interpreted as an adaptive strategy for better survival in northern populations. Findings will contribute to ensuring deployment of well-adapted genetic material and identifying tree families with enhanced disease resistance.

Place, publisher, year, edition, pages
John Wiley & Sons, 2022
Keywords
conifer, disease resistance, far-red light, immunity, latitudinal cline, light quality, local adaptation, R:FR ratio, red light, RNA sequencing
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-198228 (URN)10.1111/pce.14387 (DOI)000823001500001 ()35775408 (PubMedID)2-s2.0-85133948844 (Scopus ID)
Funder
The Kempe Foundations, JCK‐1311Swedish Research CouncilVinnova
Available from: 2022-07-21 Created: 2022-07-21 Last updated: 2023-03-24Bibliographically approved
Guillon, F., Gierlinger, N., Devaux, M.-F. & Gorzsás, A. (2022). In situ imaging of lignin and related compounds by Raman, Fourier-transform infrared (FTIR) and fluorescence microscopy. Advances in Botanical Research, 104, 215-270
Open this publication in new window or tab >>In situ imaging of lignin and related compounds by Raman, Fourier-transform infrared (FTIR) and fluorescence microscopy
2022 (English)In: Advances in Botanical Research, ISSN 0065-2296, E-ISSN 2162-5948, Vol. 104, p. 215-270Article in journal (Refereed) Published
Abstract [en]

Spectral imaging technologies simultaneously record spectral and spatial information about plant tissues in a noninvasive way. Differences in techniques result in different selection rules and spatial resolutions. This article introduces the basic principles of Raman, Fourier-transform Infrared (FTIR) and autofluorescence imaging and finally compares their strength and drawbacks. The methods result in spectral datasets as bases for image generation. Spectral preprocessing together with univariate and multivariate data analysis approaches are essential for informative lignin imaging and analysis and therefore also briefly illustrated and discussed. Examples of imaging lignin and other aromatic components in a broad range of plant tissues show the potential as well as the limitations of microspectroscopic imaging.

Place, publisher, year, edition, pages
Elsevier, 2022
Keywords
Autofluorescence imaging, FTIR imaging, Hyperspectral imaging, Multispectral imaging, Multivariate analysis, Raman imaging
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:umu:diva-193815 (URN)10.1016/bs.abr.2022.03.009 (DOI)000893291500007 ()2-s2.0-85127496928 (Scopus ID)
Available from: 2022-05-05 Created: 2022-05-05 Last updated: 2025-04-24Bibliographically approved
Chambi, D., Lundqvist, J., Nygren, E., Romero-Soto, L., Marin, K., Gorzsás, A., . . . Martín, C. (2022). Production of Exopolysaccharides by Cultivation of Halotolerant Bacillus atrophaeus BU4 in Glucose-and Xylose-Based Synthetic Media and in Hydrolysates of Quinoa Stalks. Fermentation, 8(2), Article ID 79.
Open this publication in new window or tab >>Production of Exopolysaccharides by Cultivation of Halotolerant Bacillus atrophaeus BU4 in Glucose-and Xylose-Based Synthetic Media and in Hydrolysates of Quinoa Stalks
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2022 (English)In: Fermentation, E-ISSN 2311-5637, Vol. 8, no 2, article id 79Article in journal (Refereed) Published
Abstract [en]

A halotolerant, exopolysaccharide-producing bacterium isolated from the Salar de Uyuni salt flat in Bolivia was identified as Bacillus atrophaeus using next-generation sequencing. Comparisons indicate that the genome most likely (p-value: 0.0024) belongs to a subspecies previously not represented in the database. The growth of the bacterial strain and its ability to produce exopolysaccharides (EPS) in synthetic media with glucose or xylose as carbon sources, and in hydrolysates of quinoa stalks, was investigated. The strain grew well in all synthetic media, but the growth in glucose was better than that in xylose. Sugar consumption was better when initial concentrations were low. The growth was good in enzymatically produced cellulosic hydrolysates but was inhibited in hemicellulosic hydrolysates produced using hydrothermal pretreatment. The EPS yields were up to 0.064 g/g on initial glucose and 0.047 g/g on initial xylose, and was higher in media with relatively low sugar concentrations. The EPS was isolated and purified by a sequential procedure including centrifugation, cold ethanol precipitation, trichloroacetic acid treatment, dialysis, and freeze-drying. Glucose and mannose were the main sugars identified in hydrolyzed EPS. The EPS was characterized by size-exclusion chromatography, Fouriertransform infrared (FTIR) spectroscopy, heteronuclear single-quantum coherence nuclear magnetic resonance (HSQC NMR) spectroscopy, scanning electron microscopy, X-ray diffraction, and thermogravimetric analysis. No major differences were elucidated between EPS resulting from cultivations in glucoseor-xylose-based synthetic media, while some divergences with regard to molecular-weight averages and FTIR and HSQC NMR spectra were detected for EPS from hydrolysate-based media.

Place, publisher, year, edition, pages
MDPI, 2022
Keywords
Bacillus atrophaeus, Exopolysaccharide, Genome sequencing, Halotolerant bacterium, Lignocellulose bioconversion, Quinoa stalk
National Category
Bioprocess Technology
Identifiers
urn:nbn:se:umu:diva-192785 (URN)10.3390/fermentation8020079 (DOI)000871905700001 ()2-s2.0-85124840517 (Scopus ID)
Available from: 2022-03-02 Created: 2022-03-02 Last updated: 2023-09-05Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-2298-8844

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