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Mei, Ya-Fang
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Publications (10 of 28) Show all publications
Jing, S., Zhang, J., Cao, M., Liu, M., Yan, Y., Zhao, S., . . . Zhang, Q. (2019). Household Transmission of Human Adenovirus Type 55 in Case of Fatal Acute Respiratory Disease [Letter to the editor]. Emerging Infectious Diseases, 25(9), 1756-1758
Open this publication in new window or tab >>Household Transmission of Human Adenovirus Type 55 in Case of Fatal Acute Respiratory Disease
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2019 (English)In: Emerging Infectious Diseases, ISSN 1080-6040, E-ISSN 1080-6059, Vol. 25, no 9, p. 1756-1758Article in journal, Letter (Refereed) Published
Abstract [en]

We identified a case of fatal acute respiratory disease from household transmission of human adenovirus type 55 (HAdV-55) in Anhui Province, China. Computed tomography showed severe pneumonia. Comparative genomic analysis of HAdV-55 indicated the virus possibly originated in Shanxi Province, China. More attention should be paid to highly contagious HAdV-55.

National Category
Infectious Medicine
Identifiers
urn:nbn:se:umu:diva-163658 (URN)10.3201/eid2509.181937 (DOI)000482626000025 ()31441750 (PubMedID)
Available from: 2019-10-21 Created: 2019-10-21 Last updated: 2019-10-21Bibliographically approved
Keib, A., Mei, Y.-F., Cicin-Sain, L., Busch, D. H. & Dennehy, K. M. (2019). Measuring Antiviral Capacity of T Cell Responses to Adenovirus. Journal of Immunology, 202(2), 618-624
Open this publication in new window or tab >>Measuring Antiviral Capacity of T Cell Responses to Adenovirus
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2019 (English)In: Journal of Immunology, ISSN 0022-1767, E-ISSN 1550-6606, Vol. 202, no 2, p. 618-624Article in journal (Refereed) Published
Abstract [en]

Adenoviruses are a major cause of infectious mortality in children following allogeneic hematopoietic stem cell transplantation, with adoptive transfer of adenovirus-specific T cells being an effective therapeutic approach. We have previously shown that T cells specific for the peptide epitope LTDLGQNLLY were protective. In this study, we aimed to establish a viral dissemination assay to measure the antiviral capacity of T cells specific for this and other peptide epitopes in an infectious setting. We used replication-competent adenovirus 11 (Ad11pGFP) and adenovirus 5 containing adenovirus 35 fiber (Ad5F35GFP) viruses and T cells specific for HLA-A*01-restricted LTDLGQNLLY, HLA-B*07-restricted KPYSGTAYNAL, and HLA-A*02-restricted LLDQLIEEV peptide epitopes. T cells in PBMC from healthy donors were expanded with peptide and IL-2 or treated with IL-2 alone to serve as nonstimulated control cells, and then these expanded or nonstimulated CD8(+) cells were purified and cocultured with autologous monocytes infected with adenovirus at low multiplicity of infection. After 3 d, the number of infected GFP(+) monocytes and, hence, viral dissemination was quantified by flow cytometry. T cells expanded with LTDLGQNLLY peptide from multiple HLA-A*01(+) donors prevented adenovirus dissemination, and nonstimulated T cells did not prevent dissemination, thus, indicating that LTDLGQNLLY-specific T cells have high antiviral capacity. Similarly, expanded KPYSGTAYNAL- and LLDQLIEEV-specific T cells could prevent viral dissemination. However, the frequency of expanded T cells specific for these last two epitopes was variable between donors with consequent variable prevention of adenoviral dissemination. Taken together, we demonstrate that T cells specific for three peptide epitopes, from both structural and nonstructural proteins, can prevent adenoviral dissemination and provide a novel method to measure the antiviral capacity of adenovirus-specific T cell responses.

Place, publisher, year, edition, pages
American Association of Immunologists, 2019
National Category
Immunology in the medical area
Identifiers
urn:nbn:se:umu:diva-155759 (URN)10.4049/jimmunol.1801003 (DOI)000455041800032 ()30530481 (PubMedID)
Available from: 2019-01-28 Created: 2019-01-28 Last updated: 2019-01-28Bibliographically approved
Niittykoski, M., zu Fraunberg, M. v., Martikainen, M., Rauramaa, T., Immonen, A., Koponen, S., . . . Hinkkanen, A. (2017). Immunohistochemical Characterization and Sensitivity to Human Adenovirus Serotypes 3, 5, and 11p of New Cell Lines Derived from Human Diffuse Grade II to IV Gliomas. Translational Oncology, 10(5), 772-779
Open this publication in new window or tab >>Immunohistochemical Characterization and Sensitivity to Human Adenovirus Serotypes 3, 5, and 11p of New Cell Lines Derived from Human Diffuse Grade II to IV Gliomas
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2017 (English)In: Translational Oncology, ISSN 1944-7124, E-ISSN 1936-5233, Vol. 10, no 5, p. 772-779Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Oncolytic adenoviruses show promise in targeting gliomas because they do not replicate in normal brain cells. However, clinical responses occur only in a subset of patients. One explanation could be the heterogenic expression level of virus receptors. Another contributing factor could be variable activity of tumor antiviral defenses in different glioma subtypes. METHODS: We established a collection of primary low-passage cell lines from different glioma subtypes (3 glioblastomas, 3 oligoastrocytomas, and 2 oligodendrogliomas) and assessed them for receptor expression and sensitivity to human adenovirus (HAd) serotypes 3, 5, and 11p. To gauge the impact of antiviral defenses, we also compared the infectivity of the oncolytic adenoviruses in interferon (IFN)-pretreated cells with IFN-sensitive Semliki Forest virus (SFV). RESULTS: Immunostaining revealed generally low expression of HAd5 receptor CAR in both primary tumors and derived cell lines. HAd11p receptor CD46 levels were maintained at moderate levels in both primary tumor samples and derived cell lines. HAd3 receptor DSG-2 was reduced in the cell lines compared to the tumors. Yet, at equal multiplicities of infection, the oncolytic potency of HAd5 in vitro in tumor-derived cells was comparable to HAd11p, whereas HAd3 lysed fewer cells than either of the other two HAd serotypes in 72 hours. IFN blocked replication of SFV, while HAds were rather unaffected. CONCLUSIONS: Adenovirus receptor levels on glioma-derived cell lines did not correlate with infection efficacy and may not be a relevant indicator of clinical oncolytic potency. Adenovirus receptor analysis should be preferentially performed on biopsies obtained perioperatively.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE INC, 2017
National Category
Neurology
Identifiers
urn:nbn:se:umu:diva-140454 (URN)10.1016/j.tranon.2017.07.002 (DOI)000411278400009 ()28797937 (PubMedID)
Available from: 2017-10-26 Created: 2017-10-26 Last updated: 2018-06-09Bibliographically approved
Wu, H. D. & Mei, Y.-F. (2017). Replication-competent adenovirus 11p vector armed with ADP gene at E1 region significantly improved tumour-killing effect on metastatic prostate cells in vitro and in vivo. Paper presented at European-Society-of-Gene-and-Cell-Therapy (ESCGT) Congress, 2017, Berlin, GERMANY. Human Gene Therapy, 28(12), A29-A29
Open this publication in new window or tab >>Replication-competent adenovirus 11p vector armed with ADP gene at E1 region significantly improved tumour-killing effect on metastatic prostate cells in vitro and in vivo
2017 (English)In: Human Gene Therapy, ISSN 1043-0342, E-ISSN 1557-7422, Vol. 28, no 12, p. A29-A29Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
Mary Ann Liebert, 2017
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:umu:diva-143941 (URN)000418410700083 ()
Conference
European-Society-of-Gene-and-Cell-Therapy (ESCGT) Congress, 2017, Berlin, GERMANY
Note

Meeting Abstract: P042

Available from: 2018-01-15 Created: 2018-01-15 Last updated: 2018-06-09Bibliographically approved
Mei, Y.-F., Wu, H., Hultenby, K. & Silver, J. (2016). Complete replication-competent adenovirus 11p vectors with E1 or E3 insertions show improved heat stability. Virology, 497, 198-210
Open this publication in new window or tab >>Complete replication-competent adenovirus 11p vectors with E1 or E3 insertions show improved heat stability
2016 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 497, p. 198-210Article in journal (Refereed) Published
Abstract [en]

Conventional adenovirus vectors harboring E1 or E3 deletions followed by the insertion of an exogenous gene show considerably reduced virion stability. Here, we report strategies to generate complete replication-competent Ad11p(RCAd11p) vectors that overcome the above disadvantage. A GFP cassette was successfully introduced either upstream of E1A or in the E3A region. The resulting vectors showed high expression levels of the hexon and E1genes and also strongly induced the cytopathic effect in targeted cells. When harboring oversized genomes, the RCAd11pE1 and RCAd11pE3 vectors showed significantly improved heat stability in comparison to Ad11pwt; of the three, RCAd11pE3 was the most tolerant to heat treatment. Electron microscopy showed that RCAd11pE3, RCAd11pE1, Ad11pwt, and Ad11pE1 Delmanifested dominant, moderate, minimum, or no full virus particles after heat treatment at 47°C for 5 h. Our results demonstrated that both genome size and the insertion site in the viral genome affect virion stability.

Place, publisher, year, edition, pages
Elsevier, 2016
Keywords
Ad11p vectors, Kinetics, Infection, Morphology, Improved stability
National Category
Immunology in the medical area
Identifiers
urn:nbn:se:umu:diva-126737 (URN)10.1016/j.virol.2016.07.026 (DOI)000383922200020 ()27494367 (PubMedID)
Available from: 2016-10-20 Created: 2016-10-13 Last updated: 2018-06-09Bibliographically approved
Gokumakulapalle, M. & Mei, Y.-F. (2016). Replication-competent human adenovirus 11p vectors can propagate in Vero cells. Virology, 495, 42-51
Open this publication in new window or tab >>Replication-competent human adenovirus 11p vectors can propagate in Vero cells
2016 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 495, p. 42-51Article in journal (Refereed) Published
Abstract [en]

The use of continuous cell lines derived from the African green monkey kidney (AGMK) has led to major advances in virus vaccine development. However, to date, these cells have not been used to facilitate the creation of human adenoviruses because most human adenoviruses undergo abortive infections in them. Here, we report the susceptibility of AGMK-derived cells to adenovirus lip (Ad11p) infection. First, we showed that CD46 molecules, which act as receptors for Ad11p, are expressed in AGMK cells. We then monitored Ad11p replication by measuring GFP expression as an indicator of viral transcription. We found that AGMK-derived cells were as capable as carcinoma cells at propagating full-length replication competent Ad11p (RCAd11p) DNA. Of the AGMK cell lines tested, Vero cells had the greatest capacity for adenovirus production. Thus, AGMK cells can be used to evaluate RCAd11p-mediated gene delivery, and Vero cells can be used for the production of RCAd11pGFP vectors at relatively high yields.

Keywords
Propagate, RCAd11pGFP vectors, Vero cells, LSERT C, 1984, VIRUS RESEARCH, V1, P365, FER C, 1990, JOURNAL OF VIROLOGY, V64, P3661
National Category
Immunology
Identifiers
urn:nbn:se:umu:diva-124213 (URN)10.1016/j.virol.2016.04.029 (DOI)000378659900005 ()27176913 (PubMedID)
Available from: 2016-08-01 Created: 2016-07-28 Last updated: 2018-06-07Bibliographically approved
Strand, M., Carlsson, M., Uvell, H., Islam, K., Edlund, K., Cullman, I., . . . Almqvist, F. (2014). Isolation and characterization of anti-adenoviral secondary metabolites from marine actinobacteria. Marine Drugs, 12(2), 799-821
Open this publication in new window or tab >>Isolation and characterization of anti-adenoviral secondary metabolites from marine actinobacteria
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2014 (English)In: Marine Drugs, ISSN 1660-3397, E-ISSN 1660-3397, Vol. 12, no 2, p. 799-821Article in journal (Refereed) Published
Abstract [en]

Adenovirus infections in immunocompromised patients are associated with high mortality rates. Currently, there are no effective anti-adenoviral therapies available. It is well known that actinobacteria can produce secondary metabolites that are attractive in drug discovery due to their structural diversity and their evolved interaction with biomolecules. Here, we have established an extract library derived from actinobacteria isolated from Vestfjorden, Norway, and performed a screening campaign to discover anti-adenoviral compounds. One extract with anti-adenoviral activity was found to contain a diastereomeric 1:1 mixture of the butenolide secondary alcohols 1a and 1b. By further cultivation and analysis, we could isolate 1a and 1b in different diastereomeric ratio. In addition, three more anti-adenoviral butenolides 2, 3 and 4 with differences in their side-chains were isolated. In this study, the anti-adenoviral activity of these compounds was characterized and substantial differences in the cytotoxic potential between the butenolide analogs were observed. The most potent butenolide analog 3 displayed an EC50 value of 91 μM and no prominent cytotoxicity at 2 mM. Furthermore, we propose a biosynthetic pathway for these compounds based on their relative time of appearance and structure.

Place, publisher, year, edition, pages
MDPI, 2014
Keywords
adenovirus; antiviral; natural products; secondary metabolites; marine actinobacteria; extract screening; butenolides
National Category
Basic Medicine
Identifiers
urn:nbn:se:umu:diva-86525 (URN)10.3390/md12020799 (DOI)000335745100011 ()24477283 (PubMedID)
Available from: 2014-03-03 Created: 2014-02-28 Last updated: 2018-06-08Bibliographically approved
Strand, M., Islam, K., Edlund, K., Öberg, C. T., Allard, A., Bergström, T., . . . Wadell, G. (2012). 2-[4,5-Difluoro-2-(2-fluorobenzoylamino)-benzoylamino]benzoic acid, an antiviral compound with activity against acyclovir-resistant isolates of herpes simplex virus type 1 and 2. Antimicrobial Agents and Chemotherapy, 56(11), 5735-5743
Open this publication in new window or tab >>2-[4,5-Difluoro-2-(2-fluorobenzoylamino)-benzoylamino]benzoic acid, an antiviral compound with activity against acyclovir-resistant isolates of herpes simplex virus type 1 and 2
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2012 (English)In: Antimicrobial Agents and Chemotherapy, ISSN 0066-4804, E-ISSN 1098-6596, Vol. 56, no 11, p. 5735-5743Article in journal (Refereed) Published
Abstract [en]

Herpes simplex viruses (HSV-1 and HSV-2) are responsible for life-long latent infections in humans, with periods of viral reactivation associated with recurring ulcerations in the orofacial and genital tract. In immunosuppressed patients and neonates, HSV infections are associated with severe morbidity, and in some cases even mortality. Today, acyclovir is the standard therapy for management of HSV infections. However, the need for novel antiviral agents is apparent since HSV isolates resistant to acyclovir therapy are frequently isolated in immunosuppressed patients. In this study, we assessed the anti-HSV activity of the anti-adenoviral compounds 2-[2-(2-benzoylamino)-benzoylamino]benzoic acid, (Benzavir-1) and 2-[4,5-difluoro-2-(2-fluorobenzoylamino)-benzoylamino]benzoic acid, (Benzavir-2) on HSV-1 and HSV-2. Both compounds were active against both viruses. Importantly, Benzavir-2 had similar potency to acyclovir against both HSV types and it was active against clinical acyclovir-resistant HSV isolates.

Place, publisher, year, edition, pages
American Society Microbiology, 2012
Keywords
Herpes simplex virus, HSV, inhibitor, 2-[2-(benzoylamino)benzoylamino]benzoic acid, antiviral, Benzavir
National Category
Infectious Medicine
Identifiers
urn:nbn:se:umu:diva-60354 (URN)10.1128/AAC.01072-12 (DOI)000310055800039 ()22908173 (PubMedID)
Available from: 2012-10-09 Created: 2012-10-09 Last updated: 2018-06-08Bibliographically approved
Mei, Y.-f., Harrach, B. & Wadell, G. (2012). Mastadenovirus: adenoviridae. In: Christian Tidona, Gholamreza Darai (Ed.), The Springer Index of Viruses (pp. 33-48). New York: Springer Science+Business Media B.V.
Open this publication in new window or tab >>Mastadenovirus: adenoviridae
2012 (English)In: The Springer Index of Viruses / [ed] Christian Tidona, Gholamreza Darai, New York: Springer Science+Business Media B.V., 2012, p. 33-48Chapter in book (Refereed)
Place, publisher, year, edition, pages
New York: Springer Science+Business Media B.V., 2012
Keywords
medical virology, biomedicine, virology, microbiology
National Category
Microbiology in the medical area
Identifiers
urn:nbn:se:umu:diva-55181 (URN)10.1007/978-0-387-95919-1_4 (DOI)978-0-387-95919-1 (ISBN)
Available from: 2012-05-10 Created: 2012-05-10 Last updated: 2018-06-08Bibliographically approved
Öberg, C. T., Strand, M., Andersson, E. K., Edlund, K., Tran, N. P., Mei, Y.-F., . . . Elofsson, M. (2012). Synthesis, biological evaluation, and structure-activity relationships of 2-[2-(benzoylamino)benzoylamino]benzoic acid analogues as inhibitors of adenovirus replication. Journal of Medicinal Chemistry, 55(7), 3170-3181
Open this publication in new window or tab >>Synthesis, biological evaluation, and structure-activity relationships of 2-[2-(benzoylamino)benzoylamino]benzoic acid analogues as inhibitors of adenovirus replication
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2012 (English)In: Journal of Medicinal Chemistry, ISSN 0022-2623, E-ISSN 1520-4804, Vol. 55, no 7, p. 3170-3181Article in journal (Refereed) Published
Abstract [en]

2-[2-Benzoylamino)benzoylamino]benzoic acid (1) was previously identified as a potent and nontoxic antiadenoviral compound ( Antimicrob. Agents Chemother. 2010 , 54 , 3871 ). Here, the potency of 1 was improved over three generations of compounds. We found that the ortho, ortho substituent pattern and the presence of the carboxylic acid of 1 are favorable for this class of compounds and that the direction of the amide bonds (as in 1) is obligatory. Some variability in the N-terminal moiety was tolerated, but benzamides appear to be preferred. The substituents on the middle and C-terminal rings were varied, resulting in two potent inhibitors, 35g and 35j, with EC(50) = 0.6 μM and low cell toxicity.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2012
Keywords
stem-cell transplantation; immunocompromised host; formazan assay; infection; pcr; recipients; reduction; cidofovir
National Category
Chemical Sciences
Identifiers
urn:nbn:se:umu:diva-54029 (URN)10.1021/jm201636v (DOI)000302591100027 ()22369233 (PubMedID)
Available from: 2012-04-12 Created: 2012-04-12 Last updated: 2018-06-08Bibliographically approved
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