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Nording, Malin L.
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Publications (10 of 46) Show all publications
Bever, C. S., Rand, A. A., Nording, M. L., Taft, D., Kalanetra, K. M., Mills, D. A., . . . Hammock, B. D. (2018). Effects of triclosan in breast milk on the infant fecal microbiome. Chemosphere, 203, 467-473
Open this publication in new window or tab >>Effects of triclosan in breast milk on the infant fecal microbiome
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2018 (English)In: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 203, p. 467-473Article in journal (Refereed) Published
Abstract [en]

Triclosan is frequently used for its antimicrobial properties and has been detected in human serum, urine, and breast milk. Animal and molecular studies have shown that triclosan exerts a wide range of adverse health effects at both high (ppm) and low (ppb) concentrations. Since triclosan is of growing concern to human and environmental health, there is a need to improve extraction procedures and to study additional effects from triclosan exposure. In this study, we have improved triclosan extraction from breast milk by using salt (MgSO4) to reduce emulsion formation and increase water polarity and water (similar to 80%) to enhance the overall extraction efficiency (similar to 3.5 fold). This extraction method was applied to breast milk samples collected from donors who i) recorded their use of triclosan-containing personal care products and ii) provided matching infant stool samples. Of the participants who had detectable amounts of triclosan in their breast milk, nine (75%) of them reported daily use of triclosan-containing personal care products. Levels of triclosan in breast milk were compared to the donor's infant's fecal microbiome. We found that the bacterial diversity in the fecal microbiome of the infants exposed to breast milk with detectable triclosan levels differed compared to their peers exposed to milk containing non-detectable amounts. This finding implies that exogenous chemicals are impacting microbiome diversity.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Triclosan, Breast milk, Fecal microbiome, Infant, Personal care products
National Category
Environmental Sciences
Identifiers
urn:nbn:se:umu:diva-150767 (URN)10.1016/j.chemosphere.2018.03.186 (DOI)000432235000054 ()29635158 (PubMedID)
Available from: 2018-08-31 Created: 2018-08-31 Last updated: 2018-12-11Bibliographically approved
Nording, M. L. (2018). Figuring out how I belong. Science, 359(6383), 1558-1558
Open this publication in new window or tab >>Figuring out how I belong
2018 (English)In: Science, ISSN 0036-8075, E-ISSN 1095-9203, Vol. 359, no 6383, p. 1558-1558Article in journal, Editorial material (Other academic) Published
Place, publisher, year, edition, pages
American Association for the Advancement of Science, 2018
National Category
Other Social Sciences not elsewhere specified
Identifiers
urn:nbn:se:umu:diva-146561 (URN)10.1126/science.359.6383.1558 (DOI)000428657000051 ()29599248 (PubMedID)
Available from: 2018-05-16 Created: 2018-05-16 Last updated: 2018-06-09Bibliographically approved
Gouveia-Figueira, S. C., Karimpour, M., Bosson, J. A., Blomberg, A., Unosson, J., Sehlstedt, M., . . . Nording, M. L. (2018). Mass spectrometry profiling reveals altered plasma levels of monohydroxy fatty acids and related lipids in healthy humans after controlled exposure to biodiesel exhaust. Analytica Chimica Acta, 1018, 62-69
Open this publication in new window or tab >>Mass spectrometry profiling reveals altered plasma levels of monohydroxy fatty acids and related lipids in healthy humans after controlled exposure to biodiesel exhaust
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2018 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 1018, p. 62-69Article in journal (Refereed) Published
Abstract [en]

Experimental human exposure studies are an effective tool to study adverse health effects from acute inhalation of particulate matter and other constituents of air pollution. In this randomized and double-blinded crossover study, we investigated the systemic effect on bioactive lipid metabolite levels after controlled biodiesel exhaust exposure of healthy humans and compared it to filtered air at a separate exposure occasion. Eicosanoids and other oxylipins, as well as endocannabinoids and related lipids, were quantified in plasma from 14 healthy volunteers at baseline and at three subsequent time points (2, 6, and 24 h) after 1 h exposure sessions. Protocols based on liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) methods were developed to detect temporal changes in circulating levels after biodiesel exhaust exposure. The exhaust was generated by a diesel engine fed with an undiluted rapeseed methyl ester fuel. Among the 51 analyzed lipid metabolites, PGF(2 alpha), 9,10-DiHOME, 9-HODE, 5-HETE, 11-HETE, 12-HETE, and DEA displayed significant responsiveness to the biodiesel exhaust exposure as opposed to filtered air. Of these, 9-HODE and 5-HETE at 24 h survived the 10% false discovery rate cutoff (p < 0.003). Hence, the majority of the responsive lipid metabolites were monohydroxy fatty acids. We conclude that it is possible to detect alterations in circulating bioactive lipid metabolites in response to biodiesel exhaust exposure using LC-MS/MS, with emphasis on metabolites with inflammation related properties and implications on cardiovascular health and disease. These observations aid future investigations on air pollution effects, especially with regard to cardiovascular outcomes.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Oxylipin, Endocannabinoid, Eicosanoid, Mass spectrometry, Rapeseed methyl ester, Inflammation
National Category
Occupational Health and Environmental Health Respiratory Medicine and Allergy
Identifiers
urn:nbn:se:umu:diva-148622 (URN)10.1016/j.aca.2018.02.032 (DOI)000428798200008 ()29605135 (PubMedID)
Funder
Swedish Research Council, 2010-303AFA Insurance, 130320
Available from: 2018-06-26 Created: 2018-06-26 Last updated: 2018-06-26Bibliographically approved
Håkansson, I., Gouveia-Figueira, S. C., Ernerudh, J., Vrethem, M., Ghafouri, N., Ghafouri, B. & Nording, M. (2018). Oxylipins in cerebrospinal fluid in clinically isolated syndrome and relapsing remitting multiple sclerosis. Prostaglandins & other lipid mediators, 138, 41-47
Open this publication in new window or tab >>Oxylipins in cerebrospinal fluid in clinically isolated syndrome and relapsing remitting multiple sclerosis
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2018 (English)In: Prostaglandins & other lipid mediators, ISSN 1098-8823, E-ISSN 2212-196X, Vol. 138, p. 41-47Article in journal (Refereed) Published
Abstract [en]

Although oxylipins are involved in inflammation, data on these lipid mediators in multiple sclerosis are sparse. In this study, a panel of oxylipins were analysed swith liquid chromatography tandem mass spectrometry in cerebrospinal fluid (CSF) from 41 treatment naive patients with clinically isolated syndrome (CIS) or relapsing remitting MS (RRMS) and 22 healthy controls. CSF levels of 9-hydroxyoctadecadienoic acid (9-HODE) and 13-hydroxyoctadecadienoic acid (13-HODE) were significantly higher in patients than in healthy controls (9-HODE median 380 nM (interquartile range 330-450 nM) in patients and 290 nM (interquartile range 250-340 nM) in controls, 13-HODE median 930 nM (interquartile range 810-1080 nM) in patients and 690 nM (interquartile range 570-760 nM) in controls, p < 0.001 in Mann-Whitney U tests). 9-HODE and 13-HODE performed well for separation of patients and healthy controls (AUC 0.85 and 0.88, respectively, in ROC curve analysis). However, baseline CSF levels of the oxylipins did not differ between patients with signs of disease activity during one, two and four years of follow-up and patients without. In conclusion, this study indicates that 9-HODE and 13-HODE levels are increased in CSF from CIS and RRMS patients compared with healthy controls, but does not support 9-HODE or 13-HODE as prognostic biomarkers of disease activity in patients during follow-up.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Oxylipins, Hydroxyoctadecadienoic acid, Mass spectrometry, Multiple sclerosis, Clinically isolated ndrome
National Category
Rheumatology and Autoimmunity
Identifiers
urn:nbn:se:umu:diva-153141 (URN)10.1016/j.prostaglandins.2018.08.003 (DOI)000447481000006 ()30118859 (PubMedID)2-s2.0-85051681469 (Scopus ID)
Available from: 2018-11-07 Created: 2018-11-07 Last updated: 2018-11-07Bibliographically approved
Claeson, A.-S., Gouveia-Figueira, S., Häggström, J., Fowler, C. J. & Nording, M. L. (2017). Levels of oxylipins, endocannabinoids and related lipids in plasma before and after low-level exposure to acrolein in healthy individuals and individuals with chemical intolerance. Prostaglandins, Leukotrienes and Essential Fatty Acids, 121, 60-67
Open this publication in new window or tab >>Levels of oxylipins, endocannabinoids and related lipids in plasma before and after low-level exposure to acrolein in healthy individuals and individuals with chemical intolerance
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2017 (English)In: Prostaglandins, Leukotrienes and Essential Fatty Acids, ISSN 0952-3278, E-ISSN 1532-2823, Vol. 121, p. 60-67Article in journal (Refereed) Published
Abstract [en]

Oxylipins and endocannabinoids play important biological roles, including effects upon inflammation. It is not known whether the circulating levels of these lipids are affected by inhalation of the environmental pollutant acrolein. In the present study, we have investigated the consequences of low-level exposure to acrolein on oxylipin, endocannabinoid and related lipid levels in the plasma of healthy individuals and individuals with chemical intolerance (CI), an affliction with a suggested inflammatory origin. Participants were exposed twice (60 min) to heptane and a mixture of heptane and acrolein. Blood samples were collected before exposure, after and 24 h post-exposure. There were no overt effects of acrolein exposure on the oxylipin lipidome or endocannibinoids detectable in the bloodstream at the time points investigated. No relationship between basal levels or levels after exposure to acrolein and CI could be identified. This implicates a minor role of inflammatory mediators on the systemic level in CI.

Keywords
Oxylipins, Endocannabinoids, Plasma, Human exposure, Acrolein, Chemical intolerance
National Category
Occupational Health and Environmental Health
Identifiers
urn:nbn:se:umu:diva-136786 (URN)10.1016/j.plefa.2017.06.004 (DOI)000405763500009 ()
Funder
Swedish Research Council Formas, 2010-1401
Available from: 2017-06-22 Created: 2017-06-22 Last updated: 2018-06-09Bibliographically approved
Gouveia-Figueira, S., Karimpour, M., Bosson, J. A., Blomberg, A., Unosson, J., Pourazar, J., . . . Nording, M. L. (2017). Mass spectrometry profiling of oxylipins, endocannabinoids, and N-acylethanolamines in human lung lavage fluids reveals responsiveness of prostaglandin E2 and associated lipid metabolites to biodiesel exhaust exposure. Analytical and Bioanalytical Chemistry, 409(11), 2967-2980
Open this publication in new window or tab >>Mass spectrometry profiling of oxylipins, endocannabinoids, and N-acylethanolamines in human lung lavage fluids reveals responsiveness of prostaglandin E2 and associated lipid metabolites to biodiesel exhaust exposure
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2017 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 409, no 11, p. 2967-2980Article in journal (Refereed) Published
Abstract [en]

The adverse effects of petrodiesel exhaust exposure on the cardiovascular and respiratory systems are well recognized. While biofuels such as rapeseed methyl ester (RME) biodiesel may have ecological advantages, the exhaust generated may cause adverse health effects. In the current study, we investigated the responses of bioactive lipid mediators in human airways after biodiesel exhaust exposure using lipidomic profiling methods. Lipid mediator levels in lung lavage were assessed following 1-h biodiesel exhaust (average particulate matter concentration, 159 mu g/m(3)) or filtered air exposure in 15 healthy individuals in a double-blinded, randomized, controlled, crossover study design. Bronchoscopy was performed 6 h post exposure and lung lavage fluids, i.e., bronchial wash (BW) and bronchoalveolar lavage (BAL), were sequentially collected. Mass spectrometry methods were used to detect a wide array of oxylipins (including eicosanoids), endocannabinoids, Nacylethanolamines, and related lipid metabolites in the collected BWand BAL samples. Six lipids in the human lung lavage samples were altered following biodiesel exhaust exposure, three from BAL samples and three from BW samples. Of these, elevated levels of PGE2, 12,13-DiHOME, and 13-HODE, all of which were found in BAL samples, reached Bonferroni-corrected significance. This is the first study in humans reporting responses of bioactive lipids following biodiesel exhaust exposure and the most pronounced responses were seen in the more peripheral and alveolar lung compartments, reflected by BAL collection. Since the responsiveness and diagnostic value of a subset of the studied lipid metabolites were established in lavage fluids, we conclude that our mass spectrometry profiling method is useful to assess effects of human exposure to vehicle exhaust.

Place, publisher, year, edition, pages
SPRINGER HEIDELBERG, 2017
Keywords
BAL, BW, Lipidome, Air pollution, Bronchoscopy, Eicosanoid
National Category
Occupational Health and Environmental Health
Identifiers
urn:nbn:se:umu:diva-134210 (URN)10.1007/s00216-017-0243-8 (DOI)000398515900019 ()28235994 (PubMedID)
Available from: 2017-06-20 Created: 2017-06-20 Last updated: 2018-06-09Bibliographically approved
Figueira, J., Gouveia-Figueira, S., Öhman, C., Lif Holgerson, P., Nording, M. L. & Öhman, A. (2017). Metabolite quantification by NMR and LC-MS/MS reveals differences between unstimulated, stimulated, and pure parotid saliva. Journal of Pharmaceutical and Biomedical Analysis, 140, 295-300, Article ID S0731-7085(16)31308-5.
Open this publication in new window or tab >>Metabolite quantification by NMR and LC-MS/MS reveals differences between unstimulated, stimulated, and pure parotid saliva
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2017 (English)In: Journal of Pharmaceutical and Biomedical Analysis, ISSN 0731-7085, E-ISSN 1873-264X, Vol. 140, p. 295-300, article id S0731-7085(16)31308-5Article in journal (Refereed) Published
Abstract [en]

Saliva is a readily available biofluid that is sensitive to metabolic changes and can be collected through rapid and non-invasive collection procedures, and it shows great promise for clinical metabolomic studies. This work studied the metabolite composition of, and the differences between, saliva samples collected by unstimulated spitting/drooling, paraffin chewing-stimulated spitting, and parotid gland suction using targeted nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) for metabolite quantification. As applied here, these two analytical techniques provide complementary metabolite information and together extend the metabolome coverage with robust NMR quantification of soluble metabolites and sensitive targeted LC-MS/MS analysis of bioactive lipids in specific metabolic pathways. The NMR analysis was performed on ultrafiltrated (3kDa cutoff) saliva samples and resulted in a total of 45 quantified metabolites. The LC-MS/MS analysis was performed on both filtered and unfiltered samples and resulted in the quantification of two endocannabinoids (AEA and PEA) and 22 oxylipins, which at present is the most comprehensive targeted analysis of bioactive lipids in human saliva. Important differences in the metabolite composition were observed between the three saliva sample collection methods, which should be taken into consideration when designing metabolomic studies of saliva. Furthermore, the combined use of the two metabolomics platforms (NMR and LC-MS/MS) proved to be viable for research and clinical studies of the salivary metabolome.

Keywords
Eicosanoids, Endocannabinoids, LC-MS/MS, NMR, Oxylipins, Saliva
National Category
Medical and Health Sciences Natural Sciences
Identifiers
urn:nbn:se:umu:diva-134056 (URN)10.1016/j.jpba.2017.03.037 (DOI)000402850500036 ()28380387 (PubMedID)
Available from: 2017-04-26 Created: 2017-04-26 Last updated: 2018-06-09Bibliographically approved
Claeson, A.-S., Gouveia-Figueira, S., Fowler, C. J. & Nording, M. L. (2017). Perceived stress, sensory irritation and levels of prostaglandin F2 alpha in plasma after acrolein exposure: a pilot study. Paper presented at 26th Annual Meeting of the European-Chemoreception-Research-Organization (ECRO), SEP 07-10, 2016, Athens, GREECE. Chemical Senses, 42(2), E28-E28
Open this publication in new window or tab >>Perceived stress, sensory irritation and levels of prostaglandin F2 alpha in plasma after acrolein exposure: a pilot study
2017 (English)In: Chemical Senses, ISSN 0379-864X, E-ISSN 1464-3553, Vol. 42, no 2, p. E28-E28Article in journal, Meeting abstract (Refereed) Published
Place, publisher, year, edition, pages
OXFORD UNIV PRESS, 2017
National Category
Psychology
Identifiers
urn:nbn:se:umu:diva-133544 (URN)000397065700067 ()
Conference
26th Annual Meeting of the European-Chemoreception-Research-Organization (ECRO), SEP 07-10, 2016, Athens, GREECE
Available from: 2017-06-22 Created: 2017-06-22 Last updated: 2018-06-09Bibliographically approved
Gouveia-Figueira, S., Goldin, K., Hashemian, S. A., Lindberg, A., Persson, M., Nording, M. L., . . . Fowler, C. J. (2017). Plasma levels of the endocannabinoid anandamide, related N-acylethanolamines and linoleic acid-derived oxylipins in patients with migraine. Prostaglandins, Leukotrienes and Essential Fatty Acids, 120, 15-24
Open this publication in new window or tab >>Plasma levels of the endocannabinoid anandamide, related N-acylethanolamines and linoleic acid-derived oxylipins in patients with migraine
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2017 (English)In: Prostaglandins, Leukotrienes and Essential Fatty Acids, ISSN 0952-3278, E-ISSN 1532-2823, Vol. 120, p. 15-24Article in journal (Refereed) Published
Abstract [en]

There is evidence that patients with migraine have deficient levels of the endogenous cannabinoid receptor ligand anandamide (AEA). It is not known, however, if this is a localised or generalised phenomenon. In the present study, levels of AEA, related N-acylethanolamines (NAEs) and linoleic acid-derived oxylipins have been measured in the blood of 26 healthy women and 38 women with migraine (26 with aura, 12 without aura) who were matched for age and body-mass index. Blood samples were taken on two occasions: the first sample near the start of the menstrual cycle (when present) and the second approximately fourteen days later. For a subset of migraine patients, two additional blood samples were taken, one during a migraine attack and one approximately 1 month later (to be at the same stage in the menstrual cycle, when present). NAEs and oxylipins were measured by liquid chromatography coupled to mass spectrometry. Twenty-nine lipids were quantified, of which 16 were found to have a high reproducibility of measurement. There were no significant differences in the levels of AEA, the related NAEs stearoylethanolamide and oleoylethanolamide or any of the nine linoleic acid derived oxylipins measured either between migraine patients with vs. without aura, or between controls and migraine patients (after stratification to take into account whether or not the individuals had regular menstruation cycles) in either of the first two samples. Levels of linoleoylethanolamide were lower in the patients with vs. without aura on the second sample but not in the first sample, but the biological importance of this fording is unclear. Due to time-dependent increases in their concentrations ex vivo prior to centrifugation, AEA and oleoylethanolamide levels in the samples collected during migraine attacks were not analysed, but for the other fourteen lipids, there were no significant differences in plasma concentrations during migraine vs. one month later. It is concluded that migraine is not associated with a generalised (as opposed to localised) deficiency in these lipids.

Keywords
Migraine, Endocannabinoid, Anandamide, Stearoylethanolamide, Linoleoylethanolamide, Oxylipin, 9- droxy-10E, 12Z-octadecadienoic acid, Blood plasma
National Category
Other Clinical Medicine
Identifiers
urn:nbn:se:umu:diva-138050 (URN)10.1016/j.plefa.2017.04.005 (DOI)000405880800003 ()28515018 (PubMedID)
Available from: 2017-08-11 Created: 2017-08-11 Last updated: 2018-06-09Bibliographically approved
Weidemann, E., Andersson, P. L., Bidleman, T., Boman, C., Carlin, D. J., Collina, E., . . . Jansson, S. (2016). 14th congress of combustion by-products and their health effects-origin, fate, and health effects of combustion-related air pollutants in the coming era of bio-based energy sources. Environmental science and pollution research international, 23(8), 8141-8159
Open this publication in new window or tab >>14th congress of combustion by-products and their health effects-origin, fate, and health effects of combustion-related air pollutants in the coming era of bio-based energy sources
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2016 (English)In: Environmental science and pollution research international, ISSN 0944-1344, E-ISSN 1614-7499, Vol. 23, no 8, p. 8141-8159Article in journal (Refereed) Published
Abstract [en]

The 14th International Congress on Combustion By-Products and Their Health Effects was held in UmeAyen, Sweden from June 14th to 17th, 2015. The Congress, mainly sponsored by the National Institute of Environmental Health Sciences Superfund Research Program and the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning, focused on the "Origin, fate and health effects of combustion-related air pollutants in the coming era of bio-based energy sources". The international delegates included academic and government researchers, engineers, scientists, policymakers and representatives of industrial partners. The Congress provided a unique forum for the discussion of scientific advances in this research area since it addressed in combination the health-related issues and the environmental implications of combustion by-products. The scientific outcomes of the Congress included the consensus opinions that: (a) there is a correlation between human exposure to particulate matter and increased cardiac and respiratory morbidity and mortality; (b) because currently available data does not support the assessment of differences in health outcomes between biomass smoke and other particulates in outdoor air, the potential human health and environmental impacts of emerging air-pollution sources must be addressed. Assessment will require the development of new approaches to characterize combustion emissions through advanced sampling and analytical methods. The Congress also concluded the need for better and more sustainable e-waste management and improved policies, usage and disposal methods for materials containing flame retardants.

Keywords
Products of incomplete combustion, Human health, Soot, Particles, Polychlorinated dibenzo-p-dioxins, Polychlorinated dibenzofurans, Congress paper
National Category
Environmental Sciences
Identifiers
urn:nbn:se:umu:diva-121585 (URN)10.1007/s11356-016-6308-y (DOI)000374994600105 ()26906006 (PubMedID)
Projects
Bio4Energy
Available from: 2016-06-27 Created: 2016-06-03 Last updated: 2019-09-02Bibliographically approved
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