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Behndig, Anders
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Publications (10 of 82) Show all publications
Salomonsson, A., Patthey, A., Reuterswärd, C., Jönsson, M., Botling, J., Brunnström, H., . . . Planck, M. (2018). A Nation-Wide Population-Based Mapping of Targetable Alterations in Smoking-Independent Lung Cancer. Journal of Thoracic Oncology, 13(10), S431-S432
Open this publication in new window or tab >>A Nation-Wide Population-Based Mapping of Targetable Alterations in Smoking-Independent Lung Cancer
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2018 (English)In: Journal of Thoracic Oncology, ISSN 1556-0864, E-ISSN 1556-1380, Vol. 13, no 10, p. S431-S432Article in journal, Meeting abstract (Other academic) Published
Abstract [en]

Background: Smoking is by far the most important cause of lung cancer. However, lung cancer among never-smokers is common and increasing [1]. A smoking-independent subgroup of lung adenocarcinoma with certain molecular and clinical features exists [2-3]. Therefore, as 1st project within the Swedish Molecular Initiative against Lung cancer (SMIL) we aim to characterize never-smoking lung cancer for etiological, diagnostic and therapeutic purposes.

Method: Through the Swedish National Lung Cancer Registry [1], we identified all individuals who underwent surgery for lung cancer in Sweden 2005-2014 and who were registered as never-smokers (n=540). At each study site (n=6), clinical data were reviewed by a thoracic oncologist/pulmonologist through patients' medical charts and archived tumor tissues were retrieved and reviewed by a thoracic pathologist. For subsequent studies, we extracted DNA and RNA (using the Qiagen AllPrep kit for FFPE tissue) and constructed tissue microarrays. As a first pre-planned analysis, we performed fusion gene mapping using an RNA-based NanoString nCounter Elements assay, as previously described [4].

Result: In the first 212 (out of 540) analyzed samples, we detected 17 fusions involving ALK, 8 involving RET, and 2 involving NRG1. In addition, MET exon 14 skipping was found in 17 samples. In total, these findings involved 21% of analyzed cases. Additional results from further studies on the cohort will be presented.

Conclusion: SMIL is an ongoing nation-wide molecular research collaboration on lung cancer where we currently collect one of the largest never-smoking lung tumor cohorts worldwide. From the first pre-planned analyses, we conclude that, in a population-based cohort of early stage lung cancer from never-smokers, druggable oncogenic fusions are frequent.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
population-based, never-smoker, gene fusion
National Category
Cancer and Oncology Respiratory Medicine and Allergy
Identifiers
urn:nbn:se:umu:diva-155249 (URN)10.1016/j.jtho.2018.08.497 (DOI)000454014501133 ()
Available from: 2019-01-14 Created: 2019-01-14 Last updated: 2019-01-14Bibliographically approved
Skiljic, D., Petersen, A., Karlsson, J.-O., Behndig, A., Nilsson, S. & Zetterberg, M. (2018). Effects of 17-Estradiol on Activity, Gene and Protein Expression of Superoxide Dismutases in Primary Cultured Human Lens Epithelial Cells. Current Eye Research, 43(5), 639-646
Open this publication in new window or tab >>Effects of 17-Estradiol on Activity, Gene and Protein Expression of Superoxide Dismutases in Primary Cultured Human Lens Epithelial Cells
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2018 (English)In: Current Eye Research, ISSN 0271-3683, E-ISSN 1460-2202, Vol. 43, no 5, p. 639-646Article in journal (Refereed) Published
Abstract [en]

Purpose: Protective effects of estradiol against H2O2-induced oxidative stress have been demonstrated in lens epithelial cells. The purpose of this study was to investigate the effects of 17β-estradiol (E2) on the different superoxide dismutase (SOD) isoenzymes, SOD-1, SOD-2, and SOD-3, as well as estrogen receptors (ERs), ERα and ERβ, in primary cultured human lens epithelial cells (HLECs).

Materials and methods: HLECs were exposed to 0.1 µM or 1 µM E2 for 1.5 h and 24 h after which the effects were studied. Protein expression and immunolocalization of SOD-1, SOD-2, ERα, and ERβ were studied with Western blot and immunocytochemistry. Total SOD activity was measured, and gene expression analyses were performed for SOD1, SOD2, and SOD3.

Results: Increased SOD activity was seen after 1.5 h exposure to both 0.1 µM and 1 µM E2. There were no significant changes in protein or gene expression of the different SODs. Immunolabeling of SOD-1 was evident in the cytosol and nucleus; whereas, SOD-2 was localized in the mitochondria. Both ERα and ERβ were immunolocalized to the nucleus, and mitochondrial localization of ERβ was evident by colocalization with MitoTracker. Both ERα and ERβ showed altered protein expression levels after exposure to E2.

Conclusions: The observed increase in SOD activity after exposure to E2 without accompanying increase in gene or protein expression supports a role for E2 in protection against oxidative stress mediated through non-genomic mechanisms.

Place, publisher, year, edition, pages
Taylor & Francis Group, 2018
Keywords
17-estradiol, cataract, lens epithelial cells, oxidative stress, superoxide dismutase
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-147490 (URN)10.1080/02713683.2018.1437923 (DOI)000430092800010 ()29432033 (PubMedID)
Funder
Swedish Research Council, 2011-3132
Available from: 2018-05-04 Created: 2018-05-04 Last updated: 2018-06-09Bibliographically approved
Farhoudi, D. B., Behndig, A., Mollazadegan, K., Montan, P., Lundström, M. & Kugelberg, M. (2018). Spectacle use after routine cataract surgery and vision-related activity limitation. Acta Ophthalmologica, 96(6), 582-585
Open this publication in new window or tab >>Spectacle use after routine cataract surgery and vision-related activity limitation
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2018 (English)In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 96, no 6, p. 582-585Article in journal (Refereed) Published
Abstract [en]

Purpose: To explore the relationship between acquisition of new spectacles after routine cataract surgery and vision‐related activity limitation (VRAL) postoperatively.

Methods: This cohort study with intervention (survey) included 1329 patients in Sweden who had undergone a second‐eye cataract surgery during March 2013. Data from the Swedish National Cataract Register were used, including evaluations of VRAL through the Catquest‐9SF questionnaire before and 3 months after cataract surgery. Five months after the second‐eye surgery, patients completed another five‐item questionnaire about spectacle use preoperatively and postoperatively including an item on surgeons’ advice about the need for spectacles. These responses were linked to the Rasch‐analysed Catquest‐9SF data to identify correlations with VRAL.

Results: A total of 1239 patients finally participated in the study after excluding those who did not fulfil the inclusion criteria. Patients who were advised about the need for spectacles postoperatively (n = 387) had a greater (p = 0.039) improvement in the postoperative VRAL compared to patients who were not advised (n = 691). Patients who obtained new spectacles postoperatively (n = 512) also had greater improvement (p = 0.032) compared to those who did not (n = 724).

Conclusion: The average improvements in the VRAL after surgery were significantly higher for patients who obtained new distance spectacles postoperatively and for patients who were informed about the need for spectacles by their practitioners.

Place, publisher, year, edition, pages
John Wiley & Sons, 2018
Keywords
cataract surgery, PROM, self-assessed visual function, spectacle use, vision-related activity limitation
National Category
Ophthalmology
Identifiers
urn:nbn:se:umu:diva-153136 (URN)10.1111/aos.13708 (DOI)000447257100006 ()29461682 (PubMedID)
Available from: 2018-11-08 Created: 2018-11-08 Last updated: 2018-11-08Bibliographically approved
Schedin, S., Hallberg, P. & Behndig, A. (2017). Analysis of long-term visual quality with numerical 3D ray tracing after corneal crosslinking treatment. Applied Optics, 56(35), 9787-9792
Open this publication in new window or tab >>Analysis of long-term visual quality with numerical 3D ray tracing after corneal crosslinking treatment
2017 (English)In: Applied Optics, ISSN 1559-128X, E-ISSN 2155-3165, Vol. 56, no 35, p. 9787-9792Article in journal (Refereed) Published
Abstract [en]

A numerical 3D ray tracing model was used to evaluate the long-term visual effects of two regimens of corneal crosslinking (CXL) treatment of 48 patients with the corneal degeneration keratoconus. The 3D ray tracing analyses were based on corneal elevation data measured by Scheimpflug photography. Twenty-two patients were treated with standard CXL applied uniformly across the corneal surface, whereas 26 patients underwent a customized, refined treatment only at local zones on the cornea (photorefractive intrastromal CXL; PiXL). Spot diagrams, spot root-mean-square (RMS) values, and Strehl ratios were evaluated for the patients prior to and 1, 3, 6, and 12 months after treatment. It was found that the group of patients treated with PiXL, on average, tended to attain a long-term improvement of the corneal optical performance, whereas only minor changes of the optical parameters were found for group treated with standard CXL. Our results confirmed that standard CXL treatment stabilizes the corneal optical quality over time, and thus halts the progression of the corneal degeneration. In addition to stabilization, the results showed that a significantly higher proportion of subjects treated with PiXL improved in RMS, 3, 6, and 12 months after treatment, compared to with CXL (p<0.05). This finding indicates that the PiXL treatment might improve optical quality over time.

Place, publisher, year, edition, pages
Optical Society of America, 2017
Keywords
Ophthalmic optics and devices, Geometric optical design, Vision acuity, Visual optics, modeling, Visual optics, refractive anomalies
National Category
Other Physics Topics
Identifiers
urn:nbn:se:umu:diva-142716 (URN)10.1364/AO.56.009787 (DOI)000417553500023 ()29240126 (PubMedID)
Available from: 2017-12-08 Created: 2017-12-08 Last updated: 2018-06-09Bibliographically approved
Behndig, A. (2017). Corneal Collagen Crosslinking for Ectasia after Refractive Surgery. Ophthalmology (Rochester, Minn.), 124(10), 1440-1441
Open this publication in new window or tab >>Corneal Collagen Crosslinking for Ectasia after Refractive Surgery
2017 (English)In: Ophthalmology (Rochester, Minn.), ISSN 0161-6420, E-ISSN 1549-4713, Vol. 124, no 10, p. 1440-1441Article in journal, Editorial material (Other academic) Published
Place, publisher, year, edition, pages
ELSEVIER SCIENCE INC, 2017
National Category
Ophthalmology
Identifiers
urn:nbn:se:umu:diva-140451 (URN)10.1016/j.ophtha.2017.06.011 (DOI)000411130300010 ()28938922 (PubMedID)
Available from: 2017-10-26 Created: 2017-10-26 Last updated: 2018-06-09Bibliographically approved
Grzybowski, A., Schwartz, S. G., Matsuura, K., Tone, S. O., Arshinoff, S., Ng, J. Q., . . . Behndig, A. (2017). Endophthalmitis Prophylaxis in Cataract Surgery: Overview of Current Practice Patterns Around the World. Current pharmaceutical design, 23(4), 565-573
Open this publication in new window or tab >>Endophthalmitis Prophylaxis in Cataract Surgery: Overview of Current Practice Patterns Around the World
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2017 (English)In: Current pharmaceutical design, ISSN 1381-6128, E-ISSN 1873-4286, Vol. 23, no 4, p. 565-573Article, review/survey (Refereed) Published
Abstract [en]

Background: Acute-onset postoperative endophthalmitis after cataract surgery remains a rare but important cause of visual loss. There is no global consensus regarding the optimal strategies for prophylaxis of endophthalmitis and practices vary substantially around the world, especially with respect to the use of intracameral antibiotics. The European Society of Cataract & Refractive Surgeons in a randomized clinical trial (2007) reported an approximately 5-fold reduction in endophthalmitis rates associated with the use of intracameral cefuroxime. Despite this report, the use of intracameral antibiotics has not been universally adopted. Methods: Various endophthalmitis prophylaxis patterns around the world (including the United States, Canada, Australia/New Zealand, Japan, China, India, Indonesia, South Africa, Argentina, Russia, Sweden and Mexico) are compared. Each contributing author was asked to provide similar information, including endophthalmitis rates based on published studies, current practice patterns, and in some cases original survey data. Various methods were used to obtain this information, including literature reviews, expert commentary, and some new survey data not previously published. Results: Many different practice patterns were reported from around the world, specifically with respect to the use of intracameral antibiotics. Conclusion: There is no worldwide consensus regarding endophthalmitis prophylaxis with cataract surgery.

Place, publisher, year, edition, pages
BENTHAM SCIENCE PUBL LTD, 2017
Keywords
Endophthalmitis, endophthalmitis prophylaxis, intracameral antibiotics, topical antibiotics, cataract rgery, cataract surgery complications
National Category
Ophthalmology
Identifiers
urn:nbn:se:umu:diva-133479 (URN)10.2174/1381612822666161216122230 (DOI)000395685300007 ()27981903 (PubMedID)
Available from: 2017-04-11 Created: 2017-04-11 Last updated: 2018-06-09Bibliographically approved
Fredriksson, A. & Behndig, A. (2017). Measurement centration and zone diameter in anterior, posterior and total corneal astigmatism in keratoconus. Acta Ophthalmologica, 95(8), 826-833
Open this publication in new window or tab >>Measurement centration and zone diameter in anterior, posterior and total corneal astigmatism in keratoconus
2017 (English)In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 95, no 8, p. 826-833Article in journal (Refereed) Published
Abstract [en]

Purpose To investigate the central and paracentral astigmatism and the significance of centration and measurement zone diameter compared to a 3-mm pupil-centred measurement zone in keratoconus and in healthy eyes.

Methods Twenty-eight right eyes from 28 KC patients with an inferotemporal cone were selected according to specified criteria based on Oculus Pentacam HR (R) measurements and were matched with healthy control eyes. The flat (K1) and steep (K2) keratometry readings were registered from the Total Corneal Refractive Power' (TCRP) display as well as the anterior and posterior corneal astigmatism displays (ACA and PCA, respectively). Astigmatic power vectors KP0 and KP45 were calculated and analysed for a 6-mm and two 3-mm zones centred on the corneal apex and the pupil, and for 8 paracentral 3-mm zones.

Results The astigmatism was generally higher in KC. Many astigmatic values in KC differed between the 3-mm pupil-centred and the 3- and 6-mm apex-centred zones in KC. In the controls, no corresponding differences between measurement zones were seen, apart from PCA, which differed. The magnitude and direction of KP0 and KP45 varied greatly between the paracentral measurements in KC.

Conclusion Centration and measurement zone diameter have great impacts on the astigmatic values in KC. A small pupil-centred measurement zone should be considered when evaluating the astigmatism in KC.

Place, publisher, year, edition, pages
John Wiley & Sons, 2017
Keywords
anterior cornea, astigmatism, keratoconus, posterior cornea, Scheimpflug imaging
National Category
Ophthalmology
Identifiers
urn:nbn:se:umu:diva-143572 (URN)10.1111/aos.13517 (DOI)000417645900038 ()28692136 (PubMedID)
Available from: 2018-01-04 Created: 2018-01-04 Last updated: 2018-06-09Bibliographically approved
Nuijts, R. M. M., Mencucci, R., Viaud-Quentric, K., Elena, P.-P., Olmiere, C. & Behndig, A. (2017). Ocular tolerance in rabbits after intracameral administration of a fixed combination of tropicamide, phenylephrine, and lidovaine with and without rinsing. Journal of cataract and refractive surgery, 43(5), 673-679
Open this publication in new window or tab >>Ocular tolerance in rabbits after intracameral administration of a fixed combination of tropicamide, phenylephrine, and lidovaine with and without rinsing
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2017 (English)In: Journal of cataract and refractive surgery, ISSN 0886-3350, E-ISSN 1873-4502, Vol. 43, no 5, p. 673-679Article in journal (Refereed) Published
Abstract [en]

Purpose: To evaluate the safety and tolerability of a single intracameral administration of a combined mydriatic (tropicamide and phenylephrine) and anesthetic (lidocaine) formulation (Mydrane) with or without rinsing. Setting: Iris Pharma, La Gaude, France. Design: Experimental study. Methods: Sixty pigmented rabbits received 100 mu L or 200 mu L of the combination product or a placebo (sodium chloride 0.9%) by intracameral injection. For the combination product, separate groups were included with and without rinsing after administration. From day 1 day to day 7, assessments included general clinical and ocular observations, pupil diameter measurements, corneal assessments, confocal microscopy, and electroretinography (ERG). Necropsy examinations were performed at study completion at day 8. Results: Rapid mydriasis, stable 24 minutes after injection and returning to baseline levels by day 1, was induced in all groups that received the combination mydriatic and anesthetic drug. Rinsing had no effect. The combination product induced no adverse effects on the anterior or posterior segment of the eye (ie, no increased corneal thickness and endothelial cell loss, no abnormalities in ERG). Slitlamp examination showed slightly increased anterior chamber inflammation with rinsing in both the study group and placebo group. This observation was not confirmed by aqueous flare examination. No toxic effects of the products were found on histological evaluation. Conclusion: The combination mydriatic and anesthetic drug administered to pigmented rabbits as a single intracameral injection at volumes of 100 mu L and 200 mu L was well tolerated with no ocular adverse effects and no effect on the corneal endothelium.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE INC, 2017
National Category
Ophthalmology Surgery
Identifiers
urn:nbn:se:umu:diva-137818 (URN)10.1016/j.jcrs.2017.03.025 (DOI)000404088800017 ()28602331 (PubMedID)
Available from: 2017-07-20 Created: 2017-07-20 Last updated: 2018-06-09Bibliographically approved
Nordström, M., Schiller, M., Fredriksson, A. & Behndig, A. (2017). Refractive improvements and safety with topography-guided corneal crosslinking for keratoconus: 1-year results. British Journal of Ophthalmology, 101(7), 920-925
Open this publication in new window or tab >>Refractive improvements and safety with topography-guided corneal crosslinking for keratoconus: 1-year results
2017 (English)In: British Journal of Ophthalmology, ISSN 0007-1161, E-ISSN 1468-2079, Vol. 101, no 7, p. 920-925Article in journal (Refereed) Published
Abstract [en]

PURPOSE: To assess the refractive improvements and the corneal endothelial safety of an individualised topography-guided regimen for corneal crosslinking in progressive keratoconus.

METHODS: An open-label prospective randomised clinical trial was performed at the Department of Clinical Sciences, Ophthalmology, Umeå University Hospital, Umeå, Sweden. Thirty-seven patients (50 eyes) with progressive keratoconus planned for corneal crosslinking were included. The patients were randomised to topography-guided crosslinking (photorefractive intrastromal crosslinking (PiXL); n=25) or uniform 9 mm crosslinking (corneal collagen crosslinking (CXL); n=25). Visual acuity, refraction, keratometry (K1, K2 and Kmax) and corneal endothelial morphometry were assessed preoperatively and at 1, 3, 6 and 12 months postoperatively. The PiXL treatment involved an asymmetrical treatment zone centred on the area of maximum corneal steepness with treatment energies ranging from 7.2 to 15.0 J/cm(2); the CXL treatment was a uniform 9 mm 5.4 J/cm(2) pulsed crosslinking. The main outcome measures were changes in refractive errors and corneal endothelial cell density.

RESULTS: The spherical refractive errors decreased (p<0.05) and the visual acuity improved (p<0.01) at 3, 6 and 12 months after PiXL, but not after CXL. The between-groups differences, however, were not significant. K2 and Kmax decreased at 3, 6 and 12 months after PiXL (p<0.01), but not after CXL (p<0.01 when comparing the two treatments). No corneal endothelial cell loss was seen after either treatment.

CONCLUSIONS: Individualised topography-based crosslinking treatment centred on the ectatic cone has the potential to improve the corneal shape in keratoconus with decreased spherical refractive errors and improved visual acuity, without damage to the corneal endothelium.

Place, publisher, year, edition, pages
BMJ Publishing Group Ltd, 2017
Keywords
Cornea, Degeneration, Optics and Refraction
National Category
Ophthalmology
Identifiers
urn:nbn:se:umu:diva-134194 (URN)10.1136/bjophthalmol-2016-309210 (DOI)000404068600013 ()27899371 (PubMedID)
Available from: 2017-04-28 Created: 2017-04-28 Last updated: 2018-06-09Bibliographically approved
Mönestam, E. & Behndig, A. (2016). Change in light scattering caused by glistenings in hydrophobic acrylic intraocular lenses from 10 to 15 years after surgery. Journal of cataract and refractive surgery, 42(6), 864-869
Open this publication in new window or tab >>Change in light scattering caused by glistenings in hydrophobic acrylic intraocular lenses from 10 to 15 years after surgery
2016 (English)In: Journal of cataract and refractive surgery, ISSN 0886-3350, E-ISSN 1873-4502, Vol. 42, no 6, p. 864-869Article in journal (Refereed) Published
Abstract [en]

PURPOSE: To analyze the long-term change in light scattering caused by glistenings in hydrophobic acrylic intraocular lenses (IOLs) and the clinical implications on high-contrast and low-contrast visual acuity.

SETTING: Department of Clinical Sciences/Ophthalmology, Umeå University Hospital, Umeå, Sweden.

DESIGN: Prospective longitudinal case series.

METHODS: The study comprised patients who had an Acrysof MA60BM IOL implanted between 1997 and 1998. Light scattering from the IOLs was assessed with digital analysis using Scheimpflug photography (Pentacam HR) from both the 10- year and 15-year follow-up. Associations were made between corrected distance visual acuity, low-contrast visual acuity (LCVA) 10.0%, LCVA 2.5%, and the degree of light scattering from the IOL.

RESULTS: At the 15-year follow-up, the majority of the 90 patients had an increased amount of light scattering in all parts measured in the IOL than the patients at the 10-year follow-up. A subgroup of 71 patients without coexisting ocular pathology was selected for the visual function analyses. There was no correlation between light scattering and dioptric power. No clinically relevant reduction in visual function was detected in eyes with more pronounced light scattering. Patients with a larger increase in total light scattering of the IOL at the 15-year follow-up had significantly worse LCVA 2.5% than at the 10-year follow-up (P = .042).

CONCLUSIONS: Glistenings continued to increase in hydrophobic acrylic IOLs from 10 to 15 years after surgery. At 15 years, most patients had severe glistenings with a high level of light scattering from the IOL. No clinically significant effect on everyday visual function was found.

FINANCIAL DISCLOSURE: Neither author has a financial or proprietary interest in any material or method mentioned.

Place, publisher, year, edition, pages
Elsevier, 2016
National Category
Ophthalmology Surgery
Identifiers
urn:nbn:se:umu:diva-134185 (URN)10.1016/j.jcrs.2016.02.047 (DOI)000379888700011 ()27373393 (PubMedID)
Available from: 2017-04-28 Created: 2017-04-28 Last updated: 2018-06-09Bibliographically approved
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