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Publications (10 of 85) Show all publications
Li, J., Zhou, X., Chen, J., Zhu, S., Mateus, A., Eliasson, P., . . . Backman, L. J. (2025). Impact of static myoblast loading on protein secretion linked to tenocyte migration. Journal of Proteome Research, 24(5), 2529-2541
Open this publication in new window or tab >>Impact of static myoblast loading on protein secretion linked to tenocyte migration
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2025 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 24, no 5, p. 2529-2541Article in journal (Refereed) Published
Abstract [en]

Exercise has been shown to promote wound healing, including tendon repair. Myokines released from the exercised muscles are believed to play a significant role in this process. In our previous study, we used an in vitro coculture and loading model to demonstrate that 2% static loading of myoblasts increased the migration and proliferation of cocultured tenocytes─two crucial aspects of wound healing. IGF-1, released from myoblasts in response to 2% static loading, was identified as a contributor to the increased proliferation. However, the factors responsible for the enhanced migration remained unknown. In the current study, we subjected myoblasts in single culture conditions to 2, 5, and 10% static loading and performed proteomic analysis of the cell supernatants. Gene Ontology (GO) analysis revealed that 2% static loading induced the secretion of NBL1, C5, and EFEMP1, which is associated with cell migration and motility. Further investigation by adding exogenous recombinant proteins to human tenocytes showed that NBL1 increased tenocyte migration but not proliferation. This effect was not observed with treatments using C5 and EFEMP1.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2025
Keywords
migration, myokines, static loading, tenocyte, wound healing
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-238095 (URN)10.1021/acs.jproteome.5c00068 (DOI)001462713100001 ()40202163 (PubMedID)2-s2.0-105002785594 (Scopus ID)
Funder
The Kempe Foundations, JCK-2032.2The Kempe Foundations, JCSMK24-00017Magnus Bergvall Foundation, 2023-466
Available from: 2025-04-30 Created: 2025-04-30 Last updated: 2025-05-16Bibliographically approved
Kasi, P. B., Opoku, H., Novikova, L. N., Wiberg, M., Kingham, P. J., Wang, J. & Novikov, L. N. (2025). Quercetin-derived carbon dots promote proliferation and migration of Schwann cells and enhance neurite outgrowth. Colloids and Surfaces B: Biointerfaces, 251, Article ID 114609.
Open this publication in new window or tab >>Quercetin-derived carbon dots promote proliferation and migration of Schwann cells and enhance neurite outgrowth
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2025 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 251, article id 114609Article in journal (Refereed) Published
Abstract [en]

Quercetin, a flavonoid known for its antioxidant properties, has recently garnered attention as a potential neuroprotective agent for treatment of the injured nervous system. The repair of peripheral nerve injuries hinges on the proliferation and migration of Schwann cells, which play a crucial role in supporting axonal growth and myelination. In this study we synthesized Quercetin-derived carbon dots (QCDs) and investigated their effects on cultured Schwann cells and the NG108-15 cell line. QCDs was obtained by solvothermal synthesis and characterized via UV–vis absorption spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The particles demonstrated significant dose-dependent free radical scavenging activity in DPPH and ABTS radical scavenging assays, supported in vitro proliferation and migration of Schwann cells, expression of neurotrophic and angiogenic growth factors, and stimulated neurite outgrowth from NG108-15 cells. Thus, QCDs could serve as a potential novel treatment strategy to promote regeneration in the injured peripheral nervous system.

Place, publisher, year, edition, pages
Elsevier, 2025
Keywords
Nanomedicine, Neurite outgrowth, Quercetin-derived carbon dots, Schwann cells, Solvothermal synthesis
National Category
Surgery Neurosciences
Identifiers
urn:nbn:se:umu:diva-237024 (URN)10.1016/j.colsurfb.2025.114609 (DOI)001446920800001 ()40073625 (PubMedID)2-s2.0-86000649475 (Scopus ID)
Funder
Vinnova, 2017-02130The Kempe Foundations, SMK-21-0015Swedish Research Council, 2020-04437Bertil & Britt Svenssons Stiftelse för Belysningsteknik, 2021höst-14
Available from: 2025-03-31 Created: 2025-03-31 Last updated: 2025-03-31Bibliographically approved
Lauvrud, A. T., Giraudo, M. V., Wiberg, R., Wiberg, M., Kingham, P. J. & Brohlin, M. (2024). The influence of xeno-free culture conditions on the angiogenic and adipogenic differentiation properties of adipose tissue-derived stem cells. Regenerative Therapy, 26, 901-910
Open this publication in new window or tab >>The influence of xeno-free culture conditions on the angiogenic and adipogenic differentiation properties of adipose tissue-derived stem cells
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2024 (English)In: Regenerative Therapy, ISSN 2352-3204, Vol. 26, p. 901-910Article in journal (Refereed) Published
Abstract [en]

Introduction: Before performing cell therapy clinical trials, it is important to understand how cells are influenced by different growth conditions and to find optimal xeno-free medium formulations. In this study we have investigated the properties of adipose tissue-derived stem cells (ASCs) cultured under xeno-free conditions.

Methods: Human lipoaspirate samples were digested to yield the stromal vascular fraction cells which were then seeded in i) Minimum Essential Medium-α (MEM-α) supplemented with 10 % (v/v) fetal bovine serum (FBS), ii) MEM-α supplemented with 2 % (v/v) human platelet lysate (PLT) or iii) PRIME-XV MSC expansion XSFM xeno-free, serum free medium (XV). Flow cytometry for ASCs markers CD73, CD90 and CD105 together with the putative pericyte marker CD146 was performed. Growth rates were monitored over multiple passages and adipogenic differentiation performed at early and expanded passage culture. Growth factor gene expression was analyzed and an in vitro angiogenesis assay performed.

Results: Cells in FBS and PLT grew at similar rates whereas the cells cultured in XV medium proliferated significantly faster up to 60 days in culture. All cultures were >98 % positive for CD73, CD90 and CD105, whereas CD146 expression was significantly higher in XV cells. Adipogenic differentiation was most pronounced in cells which had been cultured in XV medium whilst cells grown in PLT were inferior compared with cells from the FBS cultures. IGF1 gene expression was highest in cells cultured in PLT whilst cells grown in XV medium showed 10-fold lower expression compared with FBS cells. In contrast, HGF gene expression was 90-fold greater in cells cultured in XV medium compared with those cultured in FBS. Conditioned medium from XV cultured cells showed the most angiogenic activity, inducing the greatest endothelial cell network formation and maturation.

Conclusion: Culture under different conditions alters the ASCs characteristics. Since cells cultured in XV medium showed the best adipogenic and angiogenic profile this might be a preferred medium formulation for preparing cells required for reconstructive surgical applications such as cell-assisted fat grafting.

Place, publisher, year, edition, pages
Elsevier, 2024
Keywords
Cell-assisted lipotransfer, Mesenchymal stem cells, Regenerative medicine, Stem cell therapy, Xeno-free
National Category
Medical Biotechnology
Identifiers
urn:nbn:se:umu:diva-230771 (URN)10.1016/j.reth.2024.09.013 (DOI)001338899200001 ()2-s2.0-85205929562 (Scopus ID)
Funder
Vinnova, 2017-02130Umeå UniversityRegion Västerbotten
Available from: 2024-10-10 Created: 2024-10-10 Last updated: 2025-04-24Bibliographically approved
Pérez-Díaz, S., Lindberg, J., Anerillas, L. O., Kingham, P. J., Sund, M., Rask, G., . . . Wiberg, R. (2024). The potential role of collagen type VII in breast cancer proliferation. Cancer Cell International, 24(1), Article ID 254.
Open this publication in new window or tab >>The potential role of collagen type VII in breast cancer proliferation
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2024 (English)In: Cancer Cell International, E-ISSN 1475-2867, Vol. 24, no 1, article id 254Article in journal (Refereed) Published
Abstract [en]

Background: Breast cancer is the most common cancer in women. Cancer cells can persist in a prolonged dormant state for years without any clinical evidence of disease creating an urgent need to better understand the molecular mechanisms leading to relapse. This study aimed to identify extracellular matrix (ECM) components associated with hypoxia-induced breast cancer dormancy. The effects of selected ECM proteins on breast cancer cell proliferation were analyzed, along with their correlation with established prognostic markers in human breast cancer tissue.

Materials and methods: Screening of extracellular matrix proteins was performed in hypoxia-induced dormant MCF-7 breast cancer cells. Proliferation of MCF-7 cells in vitro was subsequently determined in the presence of recombinant ColVII. Adipose tissue-derived mesenchymal stem cells (AdMSCs) subpopulation overexpressing ColVII were indirectly isolated by ColVII receptor integrin-α6 specific antibodies. AdMSCs- MCF-7 3D spheroid cultures were generated to model solid tumour conditions. In addition, the association between ColVII and various prognostic markers was evaluated in clinical samples of human breast cancer tissue.

Results: Dormant MCF-7 cells showed an elevated expression of ColVII while MCF-7 cells cultured on ColVII exhibited reduced proliferation in vitro. In AdMSCs-MCF-7 3D spheroids, a reduced proliferation of MCF-7 cells was observed in Int-α6+/ ColVIIhigh compared with Int-α6-/ ColVIIlow AdMSCs spheroids. In human tissue, high ColVII expression correlated to several positive prognostic markers. Staining for Cytokeratin-5 revealed that ColVIIhigh-expressing cells were predominantly myoepithelial cells.

Conclusion: ColVII is associated with reduced proliferation of breast cancer cells in vitro. ColVII is strongly expressed in myoepithelial cells and in breast cancer tissue the high ColVII expression correlates with several well-known positive prognostic markers, highlighting its potential as a prognostic marker in breast cancer.

Place, publisher, year, edition, pages
Springer Nature, 2024
Keywords
Breast cancer, Collagen type VII, Extracellular matrix, Mesenchymal stem cell
National Category
Cancer and Oncology
Identifiers
urn:nbn:se:umu:diva-228066 (URN)10.1186/s12935-024-03449-4 (DOI)001272366700002 ()2-s2.0-85199024105 (Scopus ID)
Available from: 2024-08-01 Created: 2024-08-01 Last updated: 2025-03-21Bibliographically approved
Nyström, M., Lauvrud, A.-T., Pérez-Díaz, S., Kingham, P. J. & Wiberg, R. (2023). Interaction of adipose-derived stem cells with active and dormant breast cancer cells. Paper presented at Scandinavian Association of Plastic Surgeons Congress, Reykjavik, Iceland, June 13–15, 2022. Journal of Plastic, Reconstructive & Aesthetic Surgery, 83, 69-76
Open this publication in new window or tab >>Interaction of adipose-derived stem cells with active and dormant breast cancer cells
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2023 (English)In: Journal of Plastic, Reconstructive & Aesthetic Surgery, ISSN 1748-6815, E-ISSN 1878-0539, Vol. 83, p. 69-76Article in journal (Refereed) Published
Abstract [en]

Background: Although autologous fat grafting is considered a successful method for the management of contour deformities, the fat graft could potentially induce cancer reappearance by fueling dormant breast cancer cells. Our aim was to characterize the role of adipose-derived stem cells on active and dormant breast cancer cell growth.

Methods: Cobalt chloride was used to induce dormancy in MCF-7 cancer cells. Proliferation of active and dormant cancer cells was determined in the presence of adipose-derived stem cells. A proteome array was used to detect cancer-related protein expression in the cell-conditioned medium. The migration of cancer cells was measured in response to conditioned medium from the adipose-derived stem cells.

Results: The adipose-derived stem cells showed variable effects on active MCF-7 cells growth and inhibited MCF-7 proliferation after the withdrawal of cobalt chloride. Of the 84 different proteins measured in the conditioned medium, only tenascin-C was differentially expressed in the co-cultures. MCF-7 cells alone did not express tenascin-C, whereas co-cultures between MCF-7 and adipose-derived stem cells expressed more tenascin-C versus adipose-derived stem cells alone. The conditioned medium from co-cultures significantly increased the migration of the cancer cells.

Conclusions: Adipose-derived stem cells themselves neither increased the growth or migration of cancer cells, suggesting that autologous fat grafting may be oncologically safe if reconstruction is postponed until there is no evidence of active disease. However, interactions between adipose-derived stem cells and MCF-7 cancer cells could potentially lead to the production of factors, which further promote cancer cell migration.

Place, publisher, year, edition, pages
Elsevier, 2023
Keywords
Adipose-derived stem cells (ASCs), Autologous fat grafting (AFG), Dormant breast cancer cells, Tenascin-C
National Category
Cell and Molecular Biology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:umu:diva-209546 (URN)10.1016/j.bjps.2023.05.006 (DOI)001020879400001 ()37270997 (PubMedID)2-s2.0-85160658685 (Scopus ID)
Conference
Scandinavian Association of Plastic Surgeons Congress, Reykjavik, Iceland, June 13–15, 2022
Funder
Umeå UniversityRegion Västerbotten
Available from: 2023-06-13 Created: 2023-06-13 Last updated: 2024-01-26Bibliographically approved
Anerillas, L. O., Wiberg, M., Kingham, P. J. & Kelk, P. (2023). Platelet lysate for expansion or osteogenic differentiation of bone marrow mesenchymal stem cells for 3D tissue constructs. Regenerative Therapy, 24, 298-310
Open this publication in new window or tab >>Platelet lysate for expansion or osteogenic differentiation of bone marrow mesenchymal stem cells for 3D tissue constructs
2023 (English)In: Regenerative Therapy, E-ISSN 2352-3204, Vol. 24, p. 298-310Article in journal (Refereed) Published
Abstract [en]

Background: The use of mesenchymal stem cells (MSCs) for the development of tissue-engineered constructs has advanced in recent years. However, future clinically approved products require following good manufacturing practice (GMP) guidelines. This includes using alternatives to xenogeneic-derived cell culture supplements to avoid rejection of the transplants. Consequently, human platelet lysate (PLT) has been adopted as an affordable and effective alternative to foetal bovine serum (FBS) in traditional 2D cultures. However, little is known about its effect in more advanced 3D culture systems.

Methods: We evaluated bone marrow MSCs (BMSCs) proliferation and CD marker expression in cells expanded in FBS or PLT-supplemented media. Differentiation capacity of the BMSCs expanded in the presence of the different supplements was evaluated in 3D type I collagen hydrogels. Furthermore, the effects of the supplements on the process of differentiation were analyzed by using qPCR and histological staining.

Results: Cell proliferation was greater in PLT-supplemented media versus FBS. BMSCs expanded in PLT showed similar osteogenic differentiation capacity in 3D compared with FBS expanded cells. In contrast, when cells were 3D differentiated in PLT they showed lower osteogenesis versus the traditional FBS protocol. This was also the case for adipogenic differentiation, in which FBS supplementation was superior to PLT.

Conclusions: PLT is a superior alternative to FBS for the expansion of MSCs without compromising their subsequent differentiation capacity in 3D. However, differentiation in PLT is impaired. Thus, PLT can be used to reduce the time required to expand the necessary cell numbers for development of 3D tissue engineered MSC constructs.

Place, publisher, year, edition, pages
Japanese Society of Regenerative Medicine, 2023
Keywords
3D, Foetal bovine serum, Human platelet lysate, Mesenchymal stem cells, Osteogenesis
National Category
Cell Biology
Identifiers
urn:nbn:se:umu:diva-213420 (URN)10.1016/j.reth.2023.07.011 (DOI)001057841400001 ()37588134 (PubMedID)2-s2.0-85167829822 (Scopus ID)
Funder
Region Västerbotten, 7003459Region Västerbotten, 7003589Umeå University
Available from: 2023-08-25 Created: 2023-08-25 Last updated: 2025-04-24Bibliographically approved
Li, J., Zhou, X., Chen, J., Eliasson, P., Kingham, P. J. & Backman, L. J. (2023). Secretome from myoblasts statically loaded at low intensity promotes tenocyte proliferation via the IGF-1 receptor pathway. The FASEB Journal, 37(10), Article ID e23203.
Open this publication in new window or tab >>Secretome from myoblasts statically loaded at low intensity promotes tenocyte proliferation via the IGF-1 receptor pathway
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2023 (English)In: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 37, no 10, article id e23203Article in journal (Refereed) Published
Abstract [en]

Exercise is widely recognized as beneficial for tendon healing. Recently, it has been described that muscle-derived molecules secreted in response to static exercise influence tendon healing. In this study, the optimal static loading intensity for tendon healing and the composition of secretome released by myoblasts in response to different intensities of static strain were investigated. In an in vitro coculture model, myoblasts were mechanically loaded using a Flexcell Tension System. Tenocytes were seeded on transwell inserts that allowed communication between the tenocytes and myoblasts without direct contact. Proliferation and migration assays, together with RNA sequencing, were used to determine potential cellular signaling pathways. The secretome from myoblasts exposed to 2% static loading increased the proliferation and migration of the cocultured tenocytes. RNA-seq analysis revealed that this loading condition upregulated the expression of numerous genes encoding secretory proteins, including insulin-like growth factor-1 (IGF-1). Confirmation of IGF-1 expression and secretion was carried out using qPCR and enzyme-linked immunosorbt assay (ELISA), revealing a statistically significant upregulation in response to 2% static loading in comparison to both control conditions and higher loading intensities of 5% and 10%. Addition of an inhibitor of the IGF-1 receptor (PQ401) to the tenocytes significantly reduced myoblast secretome-induced tenocyte proliferation. In conclusion, IGF-1 may be an important molecule in the statically loaded myoblast secretome, which is responsible for influencing tenocytes during exercise-induced healing.

Place, publisher, year, edition, pages
John Wiley & Sons, 2023
Keywords
IGF-1, mechanical loading, migration, muscle secretome, proliferation, tenocyte
National Category
Cell and Molecular Biology Physiotherapy
Identifiers
urn:nbn:se:umu:diva-214756 (URN)10.1096/fj.202301097R (DOI)001144986000001 ()37732638 (PubMedID)2-s2.0-85171800001 (Scopus ID)
Funder
Åke Wiberg Foundation, M20-0236Åke Wiberg Foundation, M22-0008The Kempe Foundations, JCK- 2032.2Swedish National Centre for Research in Sports, P2022-0010Swedish National Centre for Research in Sports, P2023-0011
Available from: 2023-10-18 Created: 2023-10-18 Last updated: 2025-05-16Bibliographically approved
Thomson, S. E., Ng, N. Y. .., Riehle, M. O., Kingham, P. J., Dahlin, L. B., Wiberg, M. & Hart, A. M. (2022). Bioengineered nerve conduits and wraps for peripheral nerve repair of the upper limb. Cochrane Database of Systematic Reviews (12), Article ID CD012574.
Open this publication in new window or tab >>Bioengineered nerve conduits and wraps for peripheral nerve repair of the upper limb
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2022 (English)In: Cochrane Database of Systematic Reviews, E-ISSN 1469-493X, no 12, article id CD012574Article, review/survey (Refereed) Published
Abstract [en]

This is a protocol for a Cochrane Review (Intervention). The objectives are as follows: To assess and compare the effects and complication rates of licensed bioengineered nerve conduits or wraps for surgical repair of traumatic peripheral nerve injuries of the upper limb. To compare effects and complications against the current gold surgical standard (nerve autograft).

Place, publisher, year, edition, pages
John Wiley & Sons, 2022
National Category
Neurology
Identifiers
urn:nbn:se:umu:diva-202649 (URN)10.1002/14651858.CD012574.pub2 (DOI)000949395500013 ()36477774 (PubMedID)2-s2.0-85143554403 (Scopus ID)
Available from: 2023-01-16 Created: 2023-01-16 Last updated: 2024-07-04Bibliographically approved
Kumar Kuna, V., Lundgren, A., Anerillas, L. O., Kelk, P., Brohlin, M., Wiberg, M., . . . Novikov, L. N. (2022). Efficacy of Nerve-Derived Hydrogels to Promote Axon Regeneration Is Influenced by the Method of Tissue Decellularization. International Journal of Molecular Sciences, 23(15), Article ID 8746.
Open this publication in new window or tab >>Efficacy of Nerve-Derived Hydrogels to Promote Axon Regeneration Is Influenced by the Method of Tissue Decellularization
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2022 (English)In: International Journal of Molecular Sciences, ISSN 1661-6596, E-ISSN 1422-0067, Vol. 23, no 15, article id 8746Article in journal (Refereed) Published
Abstract [en]

Injuries to large peripheral nerves are often associated with tissue defects and require reconstruction using autologous nerve grafts, which have limited availability and result in donor site morbidity. Peripheral nerve-derived hydrogels could potentially supplement or even replace these grafts. In this study, three decellularization protocols based on the ionic detergents sodium dodecyl sulfate (P1) and sodium deoxycholate (P2), or the organic solvent tri-n-butyl phosphate (P3), were used to prepare hydrogels. All protocols resulted in significantly decreased amounts of genomic DNA, but the P2 hydrogel showed the best preservation of extracellular matrix proteins, cytokines, and chemokines, and reduced levels of sulfated glycosaminoglycans. In vitro P1 and P2 hydrogels supported Schwann cell viability, secretion of VEGF, and neurite outgrowth. Surgical repair of a 10 mm-long rat sciatic nerve gap was performed by implantation of tubular polycaprolactone conduits filled with hydrogels followed by analyses using diffusion tensor imaging and immunostaining for neuronal and glial markers. The results demonstrated that the P2 hydrogel considerably increased the number of axons and the distance of regeneration into the distal nerve stump. In summary, the method used to decellularize nerve tissue affects the efficacy of the resulting hydrogels to support regeneration after nerve injury.

Place, publisher, year, edition, pages
MDPI, 2022
Keywords
MRI, biosynthetic conduit, decellularized nerve tissue, diffusion tensor imaging, nerve-derived hydrogel, peripheral nerve injury
National Category
Neurosciences Surgery Cell and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-199043 (URN)10.3390/ijms23158746 (DOI)000839268700001 ()35955880 (PubMedID)2-s2.0-85137098673 (Scopus ID)
Funder
Region VästerbottenVinnova, 2017-02130
Available from: 2022-09-01 Created: 2022-09-01 Last updated: 2022-10-03Bibliographically approved
Schaakxs, D., Wiberg, M., Kingham, P. J. & Kalbermatten, D. F. (2022). Intramuscular Stem Cell Injection in Combination with Bioengineered Nerve Repair or Nerve Grafting Reduces Muscle Atrophy. Plastic and reconstructive surgery (1963), 149(5), 905E-913E
Open this publication in new window or tab >>Intramuscular Stem Cell Injection in Combination with Bioengineered Nerve Repair or Nerve Grafting Reduces Muscle Atrophy
2022 (English)In: Plastic and reconstructive surgery (1963), ISSN 0032-1052, E-ISSN 1529-4242, Vol. 149, no 5, p. 905E-913EArticle in journal (Refereed) Published
Abstract [en]

Background: Peripheral nerve injuries represent a clinical challenge, especially when they are accompanied by loss of neural tissue. In this study, the authors attempted to attain a better outcome after a peripheral nerve injury by both repairing the nerve lesion and treating the denervated muscle at the same time.

Methods: Rat sciatic nerves were transected to create 10-mm gaps. Repair was performed in five groups (n = 5 rats for each), as follows: group 1, nerve repair using poly-3-hydroxybutyrate strips to connect the proximal and distal stumps, in combination with control growth medium injection in the gastrocnemius muscle; group 2, nerve repair with poly-3-hydroxybutyrate strip seeded with Schwann cell-like differentiated adipose stem cells (differentiated adipose stem cell strip) in combination with growth medium intramuscular injection; group 3, differentiated adipose stem cell strip in combination with intramuscular injection of differentiated adipose stem cells; group 4, repair using autograft (reverse sciatic nerve graft) in combination with intramuscular injection of growth medium; and group 5, autograft in combination with intramuscular injection of differentiated adipose stem cells. Six weeks after nerve injury, the effects of the stem cells on muscle atrophy were assessed.

Results: Poly-3-hydroxybutyrate strips seeded with differentiated adipose stem cells showed a high number of βIII-tubulin-positive axons entering the distal stump and abundant endothelial cells. Group 1 animals exhibited more muscle atrophy than all the other groups, and group 5 animals had the greatest muscle weights and muscle fibers size.

Conclusion: Bioengineering nerve repair in combination with intramuscular stem cell injection is a promising technique to treat nerve lesions and associated muscle atrophy. Clinical Relevance Statement: Nerve injuries and resulting muscle atrophy are a clinical challenge. To optimize functional recovery after a nerve lesion, the authors treated the nerve and muscle at the same time by using regenerative medicine with adipose stem cells and obtained encouraging results for future clinical applications.

Place, publisher, year, edition, pages
Lippincott Williams & Wilkins, 2022
National Category
Surgery Cell and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-194883 (URN)10.1097/PRS.0000000000009031 (DOI)000788119300008 ()35271540 (PubMedID)2-s2.0-85129244800 (Scopus ID)
Funder
Swedish Research CouncilEU, European Research CouncilRegion Västerbotten
Available from: 2022-06-07 Created: 2022-06-07 Last updated: 2023-05-09Bibliographically approved
Projects
Centre for Advanced Medical Products [2017-02130_Vinnova]; Umeå University
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-2596-5936

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