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Lakehal, A., Dob, A., Novak, O. & Bellini, C. (2019). A DAO1-Mediated Circuit Controls Auxin and Jasmonate Crosstalk Robustness during Adventitious Root Initiation in Arabidopsis. International Journal of Molecular Sciences, 20(18), Article ID 4428.
Open this publication in new window or tab >>A DAO1-Mediated Circuit Controls Auxin and Jasmonate Crosstalk Robustness during Adventitious Root Initiation in Arabidopsis
2019 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 20, no 18, article id 4428Article in journal (Refereed) Published
Abstract [en]

Adventitious rooting is a post-embryonic developmental program governed by a multitude of endogenous and environmental cues. Auxin, along with other phytohormones, integrates and translates these cues into precise molecular signatures to provide a coherent developmental output. Auxin signaling guides every step of adventitious root (AR) development from the early event of cell reprogramming and identity transitions until emergence. We have previously shown that auxin signaling controls the early events of AR initiation (ARI) by modulating the homeostasis of the negative regulator jasmonate (JA). Although considerable knowledge has been acquired about the role of auxin and JA in ARI, the genetic components acting downstream of JA signaling and the mechanistic basis controlling the interaction between these two hormones are not well understood. Here we provide evidence that COI1-dependent JA signaling controls the expression of DAO1 and its closely related paralog DAO2. In addition, we show that the dao1-1 loss of function mutant produces more ARs than the wild type, probably due to its deficiency in accumulating JA and its bioactive metabolite JA-Ile. Together, our data indicate that DAO1 controls a sensitive feedback circuit that stabilizes the auxin and JA crosstalk during ARI.

Place, publisher, year, edition, pages
MDPI, 2019
Keywords
adventitious roots, auxin, auxin oxidation, jasmonates, organogenesis
National Category
Developmental Biology
Identifiers
urn:nbn:se:umu:diva-164948 (URN)10.3390/ijms20184428 (DOI)000489100500107 ()31505771 (PubMedID)
Available from: 2019-11-05 Created: 2019-11-05 Last updated: 2019-11-06Bibliographically approved
Lakehal, A., Dob, A., Rahneshan, Z., Novak, O., Escamez, S., Strnad, M., . . . Bellini, C. (2019). A Jasmonate-mediated molecular network provides cell-reprogramming decisions for organogenesis in Arabidopsis.
Open this publication in new window or tab >>A Jasmonate-mediated molecular network provides cell-reprogramming decisions for organogenesis in Arabidopsis
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2019 (English)Manuscript (preprint) (Other academic)
National Category
Developmental Biology
Identifiers
urn:nbn:se:umu:diva-164951 (URN)
Available from: 2019-11-05 Created: 2019-11-05 Last updated: 2019-11-06
Lakehal, A., Chaabouni, S., Cavel, E., Le Hir, R., Ranjan, A., Rahneshan, Z., . . . Bellini, C. (2019). A Molecular Framework for the Control of Adventitious Rooting by TIR1/AFB2-Aux/IAA-Dependent Auxin Signaling in Arabidopsis. Molecular Plant, 12(11), 1499-1514
Open this publication in new window or tab >>A Molecular Framework for the Control of Adventitious Rooting by TIR1/AFB2-Aux/IAA-Dependent Auxin Signaling in Arabidopsis
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2019 (English)In: Molecular Plant, ISSN 1674-2052, E-ISSN 1752-9867, Vol. 12, no 11, p. 1499-1514Article in journal (Refereed) Published
Abstract [en]

In Arabidopsis thaliana, canonical auxin-dependent gene regulation is mediated by 23 transcription factors from the AUXIN RESPONSE FACTOR (ARF) family that interact with auxin/indole acetic acid repressors (Aux/IAAs), which themselves form co-receptor complexes with one of six TRANSPORT INHIBITOR1/AUXIN-SIGNALLING F-BOX (TIR1/AFB) proteins. Different combinations of co-receptors drive specific sensing outputs, allowing auxin to control a myriad of processes. ARF6 and ARF8 are positive regulators of adventitious root initiation upstream of jasmonate, but the exact auxin co-receptor complexes controlling the transcriptional activity of these proteins has remained unknown. Here, using loss-of-function mutants we show that three Aux/IAA genes, IAA6, IAA9, and IAA17, act additively in the control of adventitious root (AR) initiation. These three IAA proteins interact with ARF6 and/or ARF8 and likely repress their activity in AR development. We show that TIR1 and AFB2 are positive regulators of AR formation and TIR1 plays a dual role in the control of jasmonic acid (JA) biosynthesis and conjugation, as several JA biosynthesis genes are up-regulated in the tir1-1 mutant. These results lead us to propose that in the presence of auxin, TIR1 and AFB2 form specific sensing complexes with IAA6, IAA9, and/or IAA17 to modulate JA homeostasis and control AR initiation.

Place, publisher, year, edition, pages
Elsevier, 2019
Keywords
Arabidopsis, AuxIAA, TIR1/AFB, adventitious roots, jasmonate
National Category
Developmental Biology
Identifiers
urn:nbn:se:umu:diva-164949 (URN)10.1016/j.molp.2019.09.001 (DOI)000494946400011 ()31520787 (PubMedID)
Funder
Swedish Research CouncilVinnovaKnut and Alice Wallenberg FoundationCarl Tryggers foundation The Kempe Foundations
Available from: 2019-11-05 Created: 2019-11-05 Last updated: 2019-11-26Bibliographically approved
Brunoni, F., Ljung, K. & Bellini, C. (2019). Control of root meristem establishment in conifers. Physiologia Plantarum: An International Journal for Plant Biology, 165(1), 81-89
Open this publication in new window or tab >>Control of root meristem establishment in conifers
2019 (English)In: Physiologia Plantarum: An International Journal for Plant Biology, ISSN 0031-9317, E-ISSN 1399-3054, Vol. 165, no 1, p. 81-89Article in journal (Refereed) Published
Abstract [en]

The evolution of terrestrial plant life was made possible by the establishment of a root system, which enabled plants to migrate from aquatic to terrestrial habitats. During evolution, root organization has gradually progressed from a very simple to a highly hierarchical architecture. Roots are initiated during embryogenesis and branch afterward through lateral root formation. Additionally, adventitious roots can be formed post-embryonically from aerial organs. Induction of adventitious roots (ARs) forms the basis of the vegetative propagation via cuttings in horticulture, agriculture and forestry. This method, together with somatic embryogenesis, is routinely used to clonally multiply conifers. In addition to being utilized as propagation techniques, adventitious rooting and somatic embryogenesis have emerged as versatile models to study cellular and molecular mechanisms of embryo formation and organogenesis of coniferous species. Both formation of the embryonic root and the AR primordia require the establishment of auxin gradients within cells that coordinate the developmental response. These processes also share key elements of the genetic regulatory networks that, e.g. are triggering cell fate. This minireview gives an overview of the molecular control mechanisms associated with root development in conifers, from initiation in the embryo to post-embryonic formation in cuttings.

Place, publisher, year, edition, pages
John Wiley & Sons, 2019
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-162780 (URN)10.1111/ppl.12783 (DOI)000457925000009 ()29920700 (PubMedID)
Available from: 2019-09-04 Created: 2019-09-04 Last updated: 2019-09-04Bibliographically approved
Aubry, E., Dinant, S., Vilaine, F., Bellini, C. & Le Hir, R. (2019). Lateral Transport of Organic and Inorganic Solutes. PLANTS, 8(1), Article ID 20.
Open this publication in new window or tab >>Lateral Transport of Organic and Inorganic Solutes
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2019 (English)In: PLANTS, E-ISSN 2223-7747, Vol. 8, no 1, article id 20Article, review/survey (Refereed) Published
Abstract [en]

Organic (e.g., sugars and amino acids) and inorganic (e.g., K+, Na+, PO42−, and SO42−) solutes are transported long-distance throughout plants. Lateral movement of these compounds between the xylem and the phloem, and vice versa, has also been reported in several plant species since the 1930s, and is believed to be important in the overall resource allocation. Studies of Arabidopsis thaliana have provided us with a better knowledge of the anatomical framework in which the lateral transport takes place, and have highlighted the role of specialized vascular and perivascular cells as an interface for solute exchanges. Important breakthroughs have also been made, mainly in Arabidopsis, in identifying some of the proteins involved in the cell-to-cell translocation of solutes, most notably a range of plasma membrane transporters that act in different cell types. Finally, in the future, state-of-art imaging techniques should help to better characterize the lateral transport of these compounds on a cellular level. This review brings the lateral transport of sugars and inorganic solutes back into focus and highlights its importance in terms of our overall understanding of plant resource allocation.

Place, publisher, year, edition, pages
MDPI, 2019
Keywords
phloem, xylem, lateral transport, organic solutes, inorganic solutes
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-156608 (URN)10.3390/plants8010020 (DOI)000457463600010 ()30650538 (PubMedID)
Available from: 2019-02-20 Created: 2019-02-20 Last updated: 2019-02-20Bibliographically approved
Dinant, S., Wolff, N., De Marco, F., Vilaine, F., Gissot, L., Aubry, E., . . . Le Hir, R. (2019). Synchrotron FTIR and Raman spectroscopy provide unique spectral fingerprints for Arabidopsis floral stem vascular tissues. Journal of Experimental Botany, 70(3), 871-883
Open this publication in new window or tab >>Synchrotron FTIR and Raman spectroscopy provide unique spectral fingerprints for Arabidopsis floral stem vascular tissues
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2019 (English)In: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431, Vol. 70, no 3, p. 871-883Article in journal (Refereed) Published
Abstract [en]

Cell walls are highly complex structures that are modified during plant growth and development. For example, the development of phloem and xylem vascular cells, which participate in the transport of sugars and water as well as providing support, can be influenced by cell-specific wall composition. Here, we used synchrotron radiation-based Fourier-transform infrared (SR-FTIR) and Raman spectroscopy to analyse the cell wall composition of floral stem vascular tissues of wild-type Arabidopsis and the double-mutant sweet11-1 sweet12-1, which has impaired sugar transport. The SR-FTIR spectra showed that in addition to modified xylem cell wall composition, phloem cell walls in the double-mutant line were characterized by modified hemicellulose composition. Combining Raman spectroscopy with a classification and regression tree (CART) method identified combinations of Raman shifts that could distinguish xylem vessels and fibers. In addition, the disruption of the SWEET11 and SWEET12 genes impacted on xylem wall composition in a cell-specific manner, with changes in hemicelluloses and cellulose observed at the xylem vessel interface. These results suggest that the facilitated transport of sugars by transporters that exist between vascular parenchyma cells and conducting cells is important in ensuring correct phloem and xylem cell wall composition.

Place, publisher, year, edition, pages
Oxford University Press, 2019
Keywords
Arabidopsis, CART method, cell wall, floral stem, FTIR, multivariate analysis, phloem, Raman spectroscopy, synchrotron radiation, xylem
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-157225 (URN)10.1093/jxb/ery396 (DOI)000459350700014 ()30407539 (PubMedID)
Available from: 2019-03-20 Created: 2019-03-20 Last updated: 2019-03-20Bibliographically approved
Le Hir, R., Castelain, M., Chakraborti, D., Moritz, T., Dinant, S. & Bellini, C. (2017). AtbHLH68 transcription factor contributes to the regulation of ABA homeostasis and drought stress tolerance in Arabidopsis thaliana. Physiologia Plantarum: An International Journal for Plant Biology, 160(3), 312-327
Open this publication in new window or tab >>AtbHLH68 transcription factor contributes to the regulation of ABA homeostasis and drought stress tolerance in Arabidopsis thaliana
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2017 (English)In: Physiologia Plantarum: An International Journal for Plant Biology, ISSN 0031-9317, E-ISSN 1399-3054, Vol. 160, no 3, p. 312-327Article in journal (Refereed) Published
Abstract [en]

Basic helix-loop-helix (bHLH) transcription factors are involved in a wide range of developmental processes and in response to biotic and abiotic stresses. They represent one of the biggest families of transcription factors but only few of them have been functionally characterized. Here we report the characterization of AtbHLH68 and show that, although the knock out mutant did not have an obvious development phenotype, it was slightly more sensitive to drought stress than the Col-0, and AtbHLH68 overexpressing lines displayed defects in lateral root (LR) formation and a significant increased tolerance to drought stress, likely related to an enhanced sensitivity to abscisic acid (ABA) and/or increased ABA content. AtbHLH68 was expressed in the vascular system of Arabidopsis and its expression was modulated by exogenously applied ABA in an organ-specific manner. We showed that the expression of genes involved in ABA metabolism [AtAAO3 (AtALDEHYDE OXIDASE 3) and AtCYP707A3 (AtABSCISIC ACID 8HYDROXYLASE 3)], in ABA-related response to drought-stress (AtMYC2, AtbHLH122 and AtRD29A) or during LRs development (AtMYC2 and AtABI3) was de-regulated in the overexpressing lines. We propose that AtbHLH68 has a function in the regulation of LR elongation, and in the response to drought stress, likely through an ABA-dependent pathway by regulating directly or indirectly components of ABA signaling and/or metabolism.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2017
National Category
Plant Biotechnology
Identifiers
urn:nbn:se:umu:diva-137620 (URN)10.1111/ppl.12549 (DOI)000403458700006 ()28369972 (PubMedID)
Available from: 2017-07-10 Created: 2017-07-10 Last updated: 2018-06-09Bibliographically approved
Pacurar, D. I., Pacurar, M. L., Lakehal, A., Pacurar, A. M., Ranjan, A. & Bellini, C. (2017). The Arabidopsis Cop9 signalosome subunit 4 (CNS4) is involved in adventitious root formation. Scientific Reports, 7, Article ID 628.
Open this publication in new window or tab >>The Arabidopsis Cop9 signalosome subunit 4 (CNS4) is involved in adventitious root formation
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2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, article id 628Article in journal (Refereed) Published
Abstract [en]

The COP9 signalosome (CSN) is an evolutionary conserved multiprotein complex that regulates many aspects of plant development by controlling the activity of CULLIN-RING E3 ubiquitin ligases (CRLs). CRLs ubiquitinate and target for proteasomal degradation a vast number of specific substrate proteins involved in many developmental and physiological processes, including light and hormone signaling and cell division. As a consequence of CSN pleiotropic function, complete loss of CSN activity results in seedling lethality. Therefore, a detailed analysis of CSN physiological functions in adult Arabidopsis plants has been hampered by the early seedling lethality of csn null mutants. Here we report the identification and characterization of a viable allele of the Arabidopsis COP9 signalosome subunit 4 (CSN4). The allele, designated csn4-2035, suppresses the adventitious root (AR) phenotype of the Arabidopsis superroot2-1 mutant, potentially by altering its auxin signaling. Furthermore, we show that although the csn4-2035 mutation affects primary and lateral root (LR) formation in the 2035 suppressor mutant, CSN4 and other subunits of the COP9 complex seem to differentially control AR and LR development.

Place, publisher, year, edition, pages
Nature Publishing Group, 2017
National Category
Plant Biotechnology
Identifiers
urn:nbn:se:umu:diva-134205 (URN)10.1038/s41598-017-00744-1 (DOI)000398162600004 ()28377589 (PubMedID)
Note

Errata Scientific Reports 7:628; doi:10.1038/s41598-017-00744-1

Available from: 2017-06-21 Created: 2017-06-21 Last updated: 2019-11-05Bibliographically approved
Le Hir, R., Spinner, L., Klemens, P. A. W., Chakraborti, D., de Marco, F., Vilaine, F., . . . Bellini, C. (2015). Disruption of the Sugar Transporters AtSWEET11 and AtSWEET12 Affects Vascular Development and Freezing Tolerance in Arabidopsis [Letter to the editor]. Molecular Plant, 8(11), 1687-1690
Open this publication in new window or tab >>Disruption of the Sugar Transporters AtSWEET11 and AtSWEET12 Affects Vascular Development and Freezing Tolerance in Arabidopsis
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2015 (English)In: Molecular Plant, ISSN 1674-2052, E-ISSN 1752-9867, Vol. 8, no 11, p. 1687-1690Article in journal, Letter (Refereed) Published
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-112652 (URN)10.1016/j.molp.2015.08.007 (DOI)000365049200012 ()26358680 (PubMedID)
Available from: 2015-12-14 Created: 2015-12-11 Last updated: 2018-06-07Bibliographically approved
Bellini, C. (2014). Adventious roots. eLS
Open this publication in new window or tab >>Adventious roots
2014 (English)In: eLSArticle, review/survey (Other (popular science, discussion, etc.)) Published
Place, publisher, year, edition, pages
Chichester: John Wiley & Sons, 2014
National Category
Natural Sciences
Identifiers
urn:nbn:se:umu:diva-118597 (URN)10.1002/9780470015902.a0002061.pub2 (DOI)
Available from: 2016-03-23 Created: 2016-03-23 Last updated: 2018-06-07
Projects
Role of WBC and nodulin transmembrane proteins in phloem differentiation and functionning [2008-511_Formas]; Umeå UniversityUnravelling molecular mechanisms regulating adventitious root formation in Arabidospsis thaliana [2010-04359_VR]; Umeå UniversityControl of adventitious root initiation in Arabidopsis thaliana: toward a predictive model [2013-04083_VR]; Umeå UniversityImproving Norway spruce root system architecture for sustainable forestry [2015-923_Formas]; Umeå UniversityControl of adventitious root initiation in Arabidopsis thaliana: deciphering the increasing complexity of molecular cross-talks [2017-03841_VR]; Umeå University
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-2985-6649

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