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Aubry, E., Dinant, S., Vilaine, F., Bellini, C. & Le Hir, R. (2019). Lateral Transport of Organic and Inorganic Solutes. PLANTS, 8(1), Article ID 20.
Open this publication in new window or tab >>Lateral Transport of Organic and Inorganic Solutes
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2019 (English)In: PLANTS, E-ISSN 2223-7747, Vol. 8, no 1, article id 20Article, review/survey (Refereed) Published
Abstract [en]

Organic (e.g., sugars and amino acids) and inorganic (e.g., K+, Na+, PO42−, and SO42−) solutes are transported long-distance throughout plants. Lateral movement of these compounds between the xylem and the phloem, and vice versa, has also been reported in several plant species since the 1930s, and is believed to be important in the overall resource allocation. Studies of Arabidopsis thaliana have provided us with a better knowledge of the anatomical framework in which the lateral transport takes place, and have highlighted the role of specialized vascular and perivascular cells as an interface for solute exchanges. Important breakthroughs have also been made, mainly in Arabidopsis, in identifying some of the proteins involved in the cell-to-cell translocation of solutes, most notably a range of plasma membrane transporters that act in different cell types. Finally, in the future, state-of-art imaging techniques should help to better characterize the lateral transport of these compounds on a cellular level. This review brings the lateral transport of sugars and inorganic solutes back into focus and highlights its importance in terms of our overall understanding of plant resource allocation.

Place, publisher, year, edition, pages
MDPI, 2019
Keywords
phloem, xylem, lateral transport, organic solutes, inorganic solutes
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-156608 (URN)10.3390/plants8010020 (DOI)000457463600010 ()30650538 (PubMedID)
Available from: 2019-02-20 Created: 2019-02-20 Last updated: 2019-02-20Bibliographically approved
Dinant, S., Wolff, N., De Marco, F., Vilaine, F., Gissot, L., Aubry, E., . . . Le Hir, R. (2019). Synchrotron FTIR and Raman spectroscopy provide unique spectral fingerprints for Arabidopsis floral stem vascular tissues. Journal of Experimental Botany, 70(3), 871-883
Open this publication in new window or tab >>Synchrotron FTIR and Raman spectroscopy provide unique spectral fingerprints for Arabidopsis floral stem vascular tissues
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2019 (English)In: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431, Vol. 70, no 3, p. 871-883Article in journal (Refereed) Published
Abstract [en]

Cell walls are highly complex structures that are modified during plant growth and development. For example, the development of phloem and xylem vascular cells, which participate in the transport of sugars and water as well as providing support, can be influenced by cell-specific wall composition. Here, we used synchrotron radiation-based Fourier-transform infrared (SR-FTIR) and Raman spectroscopy to analyse the cell wall composition of floral stem vascular tissues of wild-type Arabidopsis and the double-mutant sweet11-1 sweet12-1, which has impaired sugar transport. The SR-FTIR spectra showed that in addition to modified xylem cell wall composition, phloem cell walls in the double-mutant line were characterized by modified hemicellulose composition. Combining Raman spectroscopy with a classification and regression tree (CART) method identified combinations of Raman shifts that could distinguish xylem vessels and fibers. In addition, the disruption of the SWEET11 and SWEET12 genes impacted on xylem wall composition in a cell-specific manner, with changes in hemicelluloses and cellulose observed at the xylem vessel interface. These results suggest that the facilitated transport of sugars by transporters that exist between vascular parenchyma cells and conducting cells is important in ensuring correct phloem and xylem cell wall composition.

Place, publisher, year, edition, pages
Oxford University Press, 2019
Keywords
Arabidopsis, CART method, cell wall, floral stem, FTIR, multivariate analysis, phloem, Raman spectroscopy, synchrotron radiation, xylem
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-157225 (URN)10.1093/jxb/ery396 (DOI)000459350700014 ()30407539 (PubMedID)
Available from: 2019-03-20 Created: 2019-03-20 Last updated: 2019-03-20Bibliographically approved
Le Hir, R., Castelain, M., Chakraborti, D., Moritz, T., Dinant, S. & Bellini, C. (2017). AtbHLH68 transcription factor contributes to the regulation of ABA homeostasis and drought stress tolerance in Arabidopsis thaliana. Physiologia Plantarum: An International Journal for Plant Biology, 160(3), 312-327
Open this publication in new window or tab >>AtbHLH68 transcription factor contributes to the regulation of ABA homeostasis and drought stress tolerance in Arabidopsis thaliana
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2017 (English)In: Physiologia Plantarum: An International Journal for Plant Biology, ISSN 0031-9317, E-ISSN 1399-3054, Vol. 160, no 3, p. 312-327Article in journal (Refereed) Published
Abstract [en]

Basic helix-loop-helix (bHLH) transcription factors are involved in a wide range of developmental processes and in response to biotic and abiotic stresses. They represent one of the biggest families of transcription factors but only few of them have been functionally characterized. Here we report the characterization of AtbHLH68 and show that, although the knock out mutant did not have an obvious development phenotype, it was slightly more sensitive to drought stress than the Col-0, and AtbHLH68 overexpressing lines displayed defects in lateral root (LR) formation and a significant increased tolerance to drought stress, likely related to an enhanced sensitivity to abscisic acid (ABA) and/or increased ABA content. AtbHLH68 was expressed in the vascular system of Arabidopsis and its expression was modulated by exogenously applied ABA in an organ-specific manner. We showed that the expression of genes involved in ABA metabolism [AtAAO3 (AtALDEHYDE OXIDASE 3) and AtCYP707A3 (AtABSCISIC ACID 8HYDROXYLASE 3)], in ABA-related response to drought-stress (AtMYC2, AtbHLH122 and AtRD29A) or during LRs development (AtMYC2 and AtABI3) was de-regulated in the overexpressing lines. We propose that AtbHLH68 has a function in the regulation of LR elongation, and in the response to drought stress, likely through an ABA-dependent pathway by regulating directly or indirectly components of ABA signaling and/or metabolism.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2017
National Category
Plant Biotechnology
Identifiers
urn:nbn:se:umu:diva-137620 (URN)10.1111/ppl.12549 (DOI)000403458700006 ()28369972 (PubMedID)
Available from: 2017-07-10 Created: 2017-07-10 Last updated: 2018-06-09Bibliographically approved
Pacurar, D. I., Pacurar, M. L., Lakehal, A., Pacurar, A. M., Ranjan, A. & Bellini, C. (2017). The Arabidopsis Cop9 signalosome subunit 4 (CNS4) is involved in adventitious root formation. Scientific Reports, 7, Article ID 628.
Open this publication in new window or tab >>The Arabidopsis Cop9 signalosome subunit 4 (CNS4) is involved in adventitious root formation
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2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, article id 628Article in journal (Refereed) Published
Abstract [en]

The COP9 signalosome (CSN) is an evolutionary conserved multiprotein complex that regulates many aspects of plant development by controlling the activity of CULLIN-RING E3 ubiquitin ligases (CRLs). CRLs ubiquitinate and target for proteasomal degradation a vast number of specific substrate proteins involved in many developmental and physiological processes, including light and hormone signaling and cell division. As a consequence of CSN pleiotropic function, complete loss of CSN activity results in seedling lethality. Therefore, a detailed analysis of CSN physiological functions in adult Arabidopsis plants has been hampered by the early seedling lethality of csn null mutants. Here we report the identification and characterization of a viable allele of the Arabidopsis COP9 signalosome subunit 4 (CSN4). The allele, designated csn4-2035, suppresses the adventitious root (AR) phenotype of the Arabidopsis superroot2-1 mutant, potentially by altering its auxin signaling. Furthermore, we show that although the csn4-2035 mutation affects primary and lateral root (LR) formation in the 2035 suppressor mutant, CSN4 and other subunits of the COP9 complex seem to differentially control AR and LR development.

Place, publisher, year, edition, pages
Nature Publishing Group, 2017
National Category
Plant Biotechnology
Identifiers
urn:nbn:se:umu:diva-134205 (URN)10.1038/s41598-017-00744-1 (DOI)000398162600004 ()28377589 (PubMedID)
Note

Errata Scientific Reports 7:628; doi:10.1038/s41598-017-00744-1

Available from: 2017-06-21 Created: 2017-06-21 Last updated: 2018-06-09Bibliographically approved
Le Hir, R., Spinner, L., Klemens, P. A. W., Chakraborti, D., de Marco, F., Vilaine, F., . . . Bellini, C. (2015). Disruption of the Sugar Transporters AtSWEET11 and AtSWEET12 Affects Vascular Development and Freezing Tolerance in Arabidopsis [Letter to the editor]. Molecular Plant, 8(11), 1687-1690
Open this publication in new window or tab >>Disruption of the Sugar Transporters AtSWEET11 and AtSWEET12 Affects Vascular Development and Freezing Tolerance in Arabidopsis
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2015 (English)In: Molecular Plant, ISSN 1674-2052, E-ISSN 1752-9867, Vol. 8, no 11, p. 1687-1690Article in journal, Letter (Refereed) Published
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-112652 (URN)10.1016/j.molp.2015.08.007 (DOI)000365049200012 ()26358680 (PubMedID)
Available from: 2015-12-14 Created: 2015-12-11 Last updated: 2018-06-07Bibliographically approved
Bellini, C. (2014). Adventious roots. eLS
Open this publication in new window or tab >>Adventious roots
2014 (English)In: eLSArticle, review/survey (Other (popular science, discussion, etc.)) Published
Place, publisher, year, edition, pages
Chichester: John Wiley & Sons, 2014
National Category
Natural Sciences
Identifiers
urn:nbn:se:umu:diva-118597 (URN)10.1002/9780470015902.a0002061.pub2 (DOI)
Available from: 2016-03-23 Created: 2016-03-23 Last updated: 2018-06-07
Bellini, C., Pacurar, D. I. & Perrone, I. (2014). Adventitious roots and lateral roots: similarities and differences. Annual Review of Plant Biology, 65, 639-666
Open this publication in new window or tab >>Adventitious roots and lateral roots: similarities and differences
2014 (English)In: Annual Review of Plant Biology, ISSN 1543-5008, E-ISSN 1545-2123, Vol. 65, p. 639-666Article, review/survey (Refereed) Published
Abstract [en]

In addition to its role in water and nutrient uptake, the root system is fundamentally important because it anchors a plant to its substrate. Although a wide variety of root systems exist across different species, all plants have a primary root (derived from an embryonic radicle) and different types of lateral roots. Adventitious roots, by comparison, display the same functions as lateral roots but develop from aerial tissues. In addition, they not only develop as an adaptive response to various stresses, such as wounding or flooding, but also are a key limiting component of vegetative propagation. Lateral and adventitious roots share key elements of the genetic and hormonal regulatory networks but are subject to different regulatory mechanisms. In this review, we discuss the developmental processes that give rise to lateral and adventitious roots and highlight knowledge acquired over the past few years about the mechanisms that regulate adventitious root formation.

Keywords
roots, monocotyledons, dicotyledons, phytohormones, environment, vegetative propagation
National Category
Biological Sciences
Identifiers
urn:nbn:se:umu:diva-88643 (URN)10.1146/annurev-arplant-050213-035645 (DOI)000340193000024 ()24555710 (PubMedID)978-0-8243-0665-6 (ISBN)
Available from: 2014-05-12 Created: 2014-05-12 Last updated: 2018-06-07Bibliographically approved
Pacurar, D. I., Perrone, I. & Bellini, C. (2014). Auxin is a central player in the hormone cross-talks that control adventitious rooting. Physiologia Plantarum: An International Journal for Plant Biology, 151(1), 83-96
Open this publication in new window or tab >>Auxin is a central player in the hormone cross-talks that control adventitious rooting
2014 (English)In: Physiologia Plantarum: An International Journal for Plant Biology, ISSN 0031-9317, E-ISSN 1399-3054, Vol. 151, no 1, p. 83-96Article, review/survey (Refereed) Published
Abstract [en]

Vegetative propagation of economically important woody, horticultural and agricultural species rely on an efficient adventitious root (AR) formation. The formation of ARs is a complex genetic trait regulated by the interaction of environmental and endogenous factors among which the phytohormone auxin plays an essential role. This article summarizes the current knowledge related to the intricate network through which auxin controls adventitious rooting. How auxin and recently identified auxin-related compounds affect AR formation in different plant species is discussed. Particular attention is addressed to illustrate how auxin has a central role in the hormone cross-talk leading to AR development. In parallel, we describe the molecular players involved in the control of auxin homeostasis, transport and signaling, for a better understanding of the auxin action during adventitious rooting.

Place, publisher, year, edition, pages
John Wiley & Sons, 2014
Keywords
lob-domain protein, arabidopsis-thaliana, nitric-oxide, in-vitro, indole-3-butyric acid, transcription factor, system architecture, recessive mutation, plant development, response factor
National Category
Botany
Research subject
Physiological Botany; Developmental Biology
Identifiers
urn:nbn:se:umu:diva-88642 (URN)10.1111/ppl.12171 (DOI)000334079300009 ()24547793 (PubMedID)
Available from: 2014-05-12 Created: 2014-05-12 Last updated: 2018-06-07Bibliographically approved
Mauriat, M., Petterle, A., Bellini, C. & Moritz, T. (2014). Gibberellins inhibit adventitious rooting in hybrid aspen and Arabidopsis by affecting auxin transport. The Plant Journal, 78(3), 372-384
Open this publication in new window or tab >>Gibberellins inhibit adventitious rooting in hybrid aspen and Arabidopsis by affecting auxin transport
2014 (English)In: The Plant Journal, ISSN 0960-7412, E-ISSN 1365-313X, Vol. 78, no 3, p. 372-384Article in journal (Refereed) Published
Abstract [en]

Knowledge of processes involved in adventitious rooting is important to improve both fundamental understanding of plant physiology and the propagation of numerous plants. Hybrid aspen (Populus tremula x tremuloides) plants overexpressing a key gibberellin (GA) biosynthesis gene (AtGA20ox1) grow rapidly but have poor rooting efficiency, which restricts their clonal propagation. Therefore, we investigated the molecular basis of adventitious rooting in Populus and the model plant Arabidopsis. The production of adventitious roots (ARs) in tree cuttings is initiated from the basal stem region, and involves the interplay of several endogenous and exogenous factors. The roles of several hormones in this process have been characterized, but the effects of GAs have not been fully investigated. Here, we show that a GA treatment negatively affects the numbers of ARs produced by wild-type hybrid aspen cuttings. Furthermore, both hybrid aspen plants and intact Arabidopsis seedlings overexpressing AtGA20ox1, PttGID1.1 or PttGID1.3 genes (with a 35S promoter) produce few ARs, although ARs develop from the basal stem region of hybrid aspen and the hypocotyl of Arabidopsis. In Arabidopsis, auxin and strigolactones are known to affect AR formation. Our data show that the inhibitory effect of GA treatment on adventitious rooting is not mediated by perturbation of the auxin signalling pathway, or of the strigolactone biosynthetic and signalling pathways. Instead, GAs appear to act by perturbing polar auxin transport, in particular auxin efflux in hybrid aspen, and both efflux and influx in Arabidopsis.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2014
Keywords
adventitious roots, gibberellins, Arabidopsis, Populus, jasmonate, auxin transport, auxin
National Category
Plant Biotechnology
Identifiers
urn:nbn:se:umu:diva-89214 (URN)10.1111/tpj.12478 (DOI)000334824300002 ()
Available from: 2014-05-27 Created: 2014-05-26 Last updated: 2018-06-07Bibliographically approved
Pacurar, D. I., Pacurar, M. L., Bussell, J. D., Schwambach, J., Pop, T. I., Kowalczyk, M., . . . Bellini, C. (2014). Identification of new adventitious rooting mutants amongst suppressors of the Arabidopsis thaliana superroot2 mutation. Journal of Experimental Botany, 65(6), 1605-1618
Open this publication in new window or tab >>Identification of new adventitious rooting mutants amongst suppressors of the Arabidopsis thaliana superroot2 mutation
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2014 (English)In: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431, Vol. 65, no 6, p. 1605-1618Article in journal (Refereed) Published
Abstract [en]

The plant hormone auxin plays a central role in adventitious rooting and is routinely used with many economically important, vegetatively propagated plant species to promote adventitious root initiation and development on cuttings. Nevertheless the molecular mechanisms through which it acts are only starting to emerge. The Arabidopsis superroot2-1 (sur2-1) mutant overproduces auxin and, as a consequence, develops excessive adventitious roots in the hypocotyl. In order to increase the knowledge of adventitious rooting and of auxin signalling pathways and crosstalk, this study performed a screen for suppressors of superroot2-1 phenotype. These suppressors provide a new resource for discovery of genetic players involved in auxin signalling pathways or at the crosstalk of auxin and other hormones or environmental signals. This study reports the identification and characterization of 26 sur2-1 suppressor mutants, several of which were identified as mutations in candidate genes involved in either auxin biosynthesis or signalling. In addition to confirming the role of auxin as a central regulator of adventitious rooting, superroot2 suppressors indicated possible crosstalk with ethylene signalling in this process.

Place, publisher, year, edition, pages
Oxford University Press, 2014
National Category
Natural Sciences
Identifiers
urn:nbn:se:umu:diva-88641 (URN)10.1093/jxb/eru026 (DOI)000334100400015 ()24596172 (PubMedID)
Available from: 2014-05-12 Created: 2014-05-12 Last updated: 2018-06-07Bibliographically approved
Projects
Role of WBC and nodulin transmembrane proteins in phloem differentiation and functionning [2008-511_Formas]; Umeå UniversityUnravelling molecular mechanisms regulating adventitious root formation in Arabidospsis thaliana [2010-04359_VR]; Umeå UniversityControl of adventitious root initiation in Arabidopsis thaliana: toward a predictive model [2013-04083_VR]; Umeå UniversityImproving Norway spruce root system architecture for sustainable forestry [2015-923_Formas]; Umeå UniversityControl of adventitious root initiation in Arabidopsis thaliana: deciphering the increasing complexity of molecular cross-talks [2017-03841_VR]; Umeå University
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-2985-6649

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