umu.sePublications
Change search
Link to record
Permanent link

Direct link
BETA
Lindström, Mona
Alternative names
Publications (10 of 18) Show all publications
Chandra, N., Liu, Y., Liu, J.-X., Frängsmyr, L., Wu, N., Silva, L. M., . . . Arnberg, N. (2019). Sulfated Glycosaminoglycans as Viral Decoy Receptors for Human Adenovirus Type 37. Viruses, 11(3), Article ID E247.
Open this publication in new window or tab >>Sulfated Glycosaminoglycans as Viral Decoy Receptors for Human Adenovirus Type 37
Show others...
2019 (English)In: Viruses, ISSN 1999-4915, E-ISSN 1999-4915, Vol. 11, no 3, article id E247Article in journal (Refereed) Published
Abstract [en]

Glycans on plasma membranes and in secretions play important roles in infection by many viruses. Species D human adenovirus type 37 (HAdV-D37) is a major cause of epidemic keratoconjunctivitis (EKC) and infects target cells by interacting with sialic acid (SA)-containing glycans via the fiber knob domain of the viral fiber protein. HAdV-D37 also interacts with sulfated glycosaminoglycans (GAGs), but the outcome of this interaction remains unknown. Here, we investigated the molecular requirements of HAdV-D37 fiber knob:GAG interactions using a GAG microarray and demonstrated that fiber knob interacts with a broad range of sulfated GAGs. These interactions were corroborated in cell-based assays and by surface plasmon resonance analysis. Removal of heparan sulfate (HS) and sulfate groups from human corneal epithelial (HCE) cells by heparinase III and sodium chlorate treatments, respectively, reduced HAdV-D37 binding to cells. Remarkably, removal of HS by heparinase III enhanced the virus infection. Our results suggest that interaction of HAdV-D37 with sulfated GAGs in secretions and on plasma membranes prevents/delays the virus binding to SA-containing receptors and inhibits subsequent infection. We also found abundant HS in the basement membrane of the human corneal epithelium, which may act as a barrier to sub-epithelial infection. Collectively, our findings provide novel insights into the role of GAGs as viral decoy receptors and highlight the therapeutic potential of GAGs and/or GAG-mimetics in HAdV-D37 infection.

Place, publisher, year, edition, pages
MDPI, 2019
Keywords
adenovirus, antiviral drugs, cellular receptor, decoy receptor, epidemic keratoconjunctivitis, glycosaminoglycan, tropism
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:umu:diva-158515 (URN)10.3390/v11030247 (DOI)000464389700003 ()30871026 (PubMedID)
Funder
Knut and Alice Wallenberg Foundation, KAW 2013.0019Swedish Research Council, 2018-02401Västerbotten County CouncilWellcome trust, 099197MA
Available from: 2019-04-29 Created: 2019-04-29 Last updated: 2019-08-28Bibliographically approved
Vicente, A., Byström, B., Lindström, M., Stenevi, U. & Pedrosa Domellöf, F. (2018). Aniridia-related keratopathy: structural changes in naïve and transplanted corneal buttons. PLoS ONE, 13(6), Article ID e0198822.
Open this publication in new window or tab >>Aniridia-related keratopathy: structural changes in naïve and transplanted corneal buttons
Show others...
2018 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 13, no 6, article id e0198822Article in journal (Refereed) Published
Abstract [en]

Background: To study structural changes in naive and surgically treated corneas of aniridia patients with advanced aniridia-related keratopathy (ARK).

Methods and findings: Two naive corneal buttons from patients with advanced ARK submitted to penetrating keratoplasty for the first time, one corneal button from an ARK patient that had undergone a keratolimbal allograft (KLAL), two corneal buttons from ARK patients who had previously undergone centered or decentered transplantation and were now retransplanted and two adult healthy donor control corneas were processed for immunohistochemistry. Antibodies against extracellular matrix components in the stroma and in the epithelial basement membrane (collagen I and IV, collagen receptor alpha 11 integrin and laminin alpha 3 chain), markers of fibrosis, wound healing and vascularization (fibronectin, tenascin-C, vimentin, alpha-SMA and caveolin-1), cell division (Ki-67) and macrophages (CD68) were used. Naive ARK, KLAL ARK corneas and transplanted corneal buttons presented similar histopathological changes with irregular epithelium and disruption or absence of epithelial basal membrane. There was a loss of the orderly pattern of collagen lamellae and absence of collagen I in all ARK corneas. Vascularization was revealed by the presence of caveolin-1 and collagen IV in the pannus of all ARK aniridia corneas. The changes observed in decentered and centered transplants were analogous.

Conclusions: Given the similar pathological features of all cases, conditions inherent to the host seem to play an important role on the pathophysiology of the ARK in the long run.

Place, publisher, year, edition, pages
Public Library of Science, 2018
National Category
Ophthalmology
Identifiers
urn:nbn:se:umu:diva-150386 (URN)10.1371/journal.pone.0198822 (DOI)000434786600055 ()29889891 (PubMedID)2-s2.0-85048312123 (Scopus ID)
Available from: 2018-08-06 Created: 2018-08-06 Last updated: 2019-10-18Bibliographically approved
McLoon, L. K., Vicente, A., Fitzpatrick, K. R., Lindström, M. & Pedrosa Domellöf, F. (2018). Composition, architecture, and functional implications of the connective tissue network of the extraocular muscles. Investigative Ophthalmology and Visual Science, 59(1), 322-329
Open this publication in new window or tab >>Composition, architecture, and functional implications of the connective tissue network of the extraocular muscles
Show others...
2018 (English)In: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 59, no 1, p. 322-329Article in journal (Refereed) Published
Abstract [en]

Purpose: We examined the pattern and extent of connective tissue distribution in the extraocular muscles (EOMs) and determined the ability of the interconnected connective tissues to disseminate force laterally.

Methods: Human EOMs were examined for collagens I, III, IV, and VI; fibronectin; laminin; and elastin using immunohistochemistry. Connective tissue distribution was examined with scanning electron microscopy. Rabbit EOMs were examined for levels of force transmission longitudinally and transversely using in vitro force assessment.

Results: Collagens I, III, and VI localized to the endomysium, perimysium, and epimysium. Collagen IV, fibronectin, and laminin localized to the basal lamina surrounding all myofibers. All collagens localized similarly in the orbital and global layers throughout the muscle length. Elastin had the most irregular pattern and ran longitudinally and circumferentially throughout the length of all EOMs. Scanning electron microscopy showed these elements to be extensively interconnected, from endomysium through the perimysium to the epimysium surrounding the whole muscle. In vitro physiology demonstrated force generation in the lateral dimension, presumably through myofascial transmission, which was always proportional to the force generated in the longitudinally oriented muscles.

Conclusions: A striking connective tissue matrix interconnects all the myofibers and extends, via perimysial connections, to the epimysium. These interconnections are significant and allow measurable force transmission laterally as well as longitudinally, suggesting that they may contribute to the nonlinear force summation seen in motor unit recording studies. This provides strong evidence that separate compartmental movements are unlikely as no region is independent of the rest of the muscle.

Place, publisher, year, edition, pages
Rockville: The Association for Research in Vision and Ophthalmology, 2018
Keywords
extraocular muscles, connective tissue, collagen, muscle force, scanning electron microscopy, perimysium, epimysium
National Category
Ophthalmology
Identifiers
urn:nbn:se:umu:diva-146324 (URN)10.1167/iovs.17-23003 (DOI)000425855900038 ()29346490 (PubMedID)
Available from: 2018-04-05 Created: 2018-04-05 Last updated: 2018-06-09Bibliographically approved
Storm, R. J., Persson, D. B., Skalman, L. N., Frängsmyr, L., Lindström, M., Rankin, G., . . . Arnberg, N. (2017). Human Adenovirus Type 37 Uses αVβ1 and α3β1 Integrins for Infection of Human Corneal Cells. Journal of Virology, 91(5), Article ID e02019-16.
Open this publication in new window or tab >>Human Adenovirus Type 37 Uses αVβ1 and α3β1 Integrins for Infection of Human Corneal Cells
Show others...
2017 (English)In: Journal of Virology, ISSN 0022-538X, E-ISSN 1098-5514, Vol. 91, no 5, article id e02019-16Article in journal (Refereed) Published
Abstract [en]

Epidemic keratoconjunctivitis (EKC) is a severe, contagious ocular disease that affects 20 to 40 million individuals worldwide every year. EKC is mainly caused by six types of human adenovirus (HAdV): HAdV-8, -19, -37, -53, -54, and -56. Of these, HAdV-8, -19, and -37 use sialic acid-containing glycans as cellular receptors. αVβ3, αVβ5, and a few additional integrins facilitate entry and endosomal release of other HAdVs. With the exception of a few biochemical analyses indicating that HAdV-37 can interact physically with αVβ5, little is known about the integrins used by EKC-causing HAdVs. Here, we investigated the overall integrin expression on human corneal cells and found expression of α2, α3, α6, αV, β1, and β4 subunits in human corneal in situ epithelium and/or in a human corneal epithelial (HCE) cell line but no or less accessible expression of α4, α5, β3, or β5. We also identified the integrins used by HAdV-37 through a series of binding and infection competition experiments and different biochemical approaches. Together, our data suggest that HAdV-37 uses αVβ1 and α3β1 integrins for infection of human corneal epithelial cells. Furthermore, to confirm the relevance of these integrins in the HAdV-37 life cycle, we developed a corneal multilayer tissue system and found that HAdV-37 infection correlated well with the patterns of αV, α3, and β1 integrin expression. These results provide further insight into the tropism and pathogenesis of EKC-causing HAdVs and may be of importance for future development of new antiviral drugs.IMPORTANCE Keratitis is a hallmark of EKC, which is caused by six HAdV types (HAdV-8, -19, -37, -53, -54, and -56). HAdV-37 and some other HAdV types interact with integrin αVβ5 in order to enter nonocular human cells. In this study, we found that αVβ5 is not expressed on human corneal epithelial cells, thus proposing other host factors mediate corneal infection. Here, we first characterized integrin expression patterns on corneal tissue and corneal cells. Among the integrins identified, competition binding and infection experiments and biochemical assays pointed out αVβ1 and α3β1 to be of importance for HAdV-37 infection of corneal tissue. In the absence of a good animal model for EKC-causing HAdVs, we also developed an in vitro system with multilayer HCE cells and confirmed the relevance of the suggested integrins during HAdV-37 infection.

Keywords
adenoviruses, cornea, epidemic keratoconjunctivitis, integrins
National Category
Microbiology in the medical area
Identifiers
urn:nbn:se:umu:diva-133501 (URN)10.1128/JVI.02019-16 (DOI)000394356400016 ()27974569 (PubMedID)
Available from: 2017-04-11 Created: 2017-04-11 Last updated: 2018-08-06Bibliographically approved
Pedrosa-Domellöf, F., McLoon, L. K. & Lindström, M. (2016). A sturdy connective tissue network surrounds all the extraocular muscle fibers. Paper presented at Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), MAY 01-05, 2016, Seattle, WA. Investigative Ophthalmology and Visual Science, 57(12)
Open this publication in new window or tab >>A sturdy connective tissue network surrounds all the extraocular muscle fibers
2016 (English)In: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 57, no 12Article in journal, Meeting abstract (Refereed) Published
Place, publisher, year, edition, pages
ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2016
National Category
Ophthalmology
Identifiers
urn:nbn:se:umu:diva-132852 (URN)000394210604175 ()
Conference
Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), MAY 01-05, 2016, Seattle, WA
Available from: 2017-03-29 Created: 2017-03-29 Last updated: 2018-06-09Bibliographically approved
Domellöf, F. P., Parkkonen, K., Lindström, M., Nord, H., von Hoffsten, J. & Li, Z. (2015). Desmin in extraocular muscles. Paper presented at Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), MAY 03-07, 2015, Denver, CO. Investigative Ophthalmology and Visual Science, 56(7)
Open this publication in new window or tab >>Desmin in extraocular muscles
Show others...
2015 (English)In: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 56, no 7Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
ASSOC RESEARCH VISION OPHTHALMOLOGY, 2015
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:umu:diva-111515 (URN)000362882201317 ()
Conference
Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), MAY 03-07, 2015, Denver, CO
Available from: 2015-11-24 Created: 2015-11-13 Last updated: 2018-06-07Bibliographically approved
Lindström, M., Tjust, A. E. & Domellöf, F. P. (2015). Pax7-Positive Cells/Satellite Cells in Human Extraocular Muscles. Investigative Ophthalmology and Visual Science, 56(10), 6132-6143
Open this publication in new window or tab >>Pax7-Positive Cells/Satellite Cells in Human Extraocular Muscles
2015 (English)In: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 56, no 10, p. 6132-6143Article in journal (Refereed) Published
Abstract [en]

PURPOSE. We quantified and investigated the distribution of Pax7-positive cells/satellite cells (SCs) in the human extraocular muscles (EOMs). METHODS. An immunofluorescence multiple-marker method simultaneously combining two SC markers (Pax7, NCAM), detection of the basement membrane (laminin) and cell nuclei (4',6-diamidino-2-phenylindole [DAPI]), was used on the anterior, middle, and posterior portions of EOMs from five healthy donors. Pax7-positive cell and SC content, myonuclear content, myofiber cross-sectional area, and myonuclear domain were analyzed in single cross-sections. Between 3915 and 13,536 myofibers per muscle cross-section and myofibers from the entire EOM cross-section were analyzed for quantification of Pax7-positive cells per myofiber (Pax7/F).

RESULTS. The number of Pax7/F in the human EOMs varies along the length of the muscle with twice as high Pax7/F in the anterior part of the EOMs, but within the range of what has been previously reported for normal adult limb muscles. Furthermore, there are Pax7-positive cells in positions other than the classical SC position and the myonuclear domain size of adult EOMs is noticeably smaller than that previously reported for other adult skeletal muscles.

CONCLUSIONS. Previous data on differences in Pax7-positive cell/SC abundance between EOMs and limb muscles must be reconsidered and the characteristics of different Pax7-positive cell populations further investigated. Higher numbers of Pax7-positive cells in the anterior portion of the EOMs may have a bearing for strabismus surgery involving sectioning of the muscle fibers.

Keywords
satellite cell, myoprogenitor cell, Pax7
National Category
Microbiology in the medical area
Identifiers
urn:nbn:se:umu:diva-116774 (URN)10.1167/iovs.15-16544 (DOI)000368426300057 ()26393672 (PubMedID)
Funder
Swedish Research Council, K2012-63x-20399-06-3
Available from: 2016-02-15 Created: 2016-02-11 Last updated: 2018-06-07Bibliographically approved
Radovanovic, D., Peikert, K., Lindström, M. & Pedrosa Domellöf, F. (2015). Sympathetic innervation of human muscle spindles. Journal of Anatomy, 226(6), 542-548
Open this publication in new window or tab >>Sympathetic innervation of human muscle spindles
2015 (English)In: Journal of Anatomy, ISSN 0021-8782, E-ISSN 1469-7580, Vol. 226, no 6, p. 542-548Article in journal (Refereed) Published
Abstract [en]

The aim of the present study was to investigate the presence of sympathetic innervation in human muscle spindles, using antibodies against neuropeptide Y (NPY), NPY receptors and tyrosine hydroxylase (TH). A total of 232 muscle spindles were immunohistochemically examined. NPY and NPY receptors were found on the intrafusal fibers, on the blood vessels supplying muscle spindles and on free nerve endings in the periaxial space. TH-immunoreactivity was present mainly in the spindle nerve and vessel. This is, to our knowledge, the first morphological study concerning the sympathetic innervation of the human muscle spindles. The results provide anatomical evidence for direct sympathetic innervation of the intrafusal fibers and show that sympathetic innervation is not restricted to the blood vessels supplying spindles. Knowledge about direct sympathetic innervation of the muscle spindle might expand our understanding of motor and proprioceptive dysfunction under stress conditions, for example, chronic muscle pain syndromes.

Keywords
chronic muscle pain, human skeletal muscles, muscle spindle, neuropeptide Y, neuropeptide Y receptors, sympathetic innervation, tyrosine hydroxylase
National Category
Physiology
Identifiers
urn:nbn:se:umu:diva-106121 (URN)10.1111/joa.12309 (DOI)000354884400005 ()25994126 (PubMedID)
Available from: 2015-07-14 Created: 2015-07-09 Last updated: 2018-06-07Bibliographically approved
Janbaz, A. H., Lindström, M., Liu, J. & Pedrosa-Domellöf, F. (2014). Intermediate Filaments in the Human Extraocular Muscles. Investigative Ophthalmology and Visual Science, 55(8), 5151-5159
Open this publication in new window or tab >>Intermediate Filaments in the Human Extraocular Muscles
2014 (English)In: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 55, no 8, p. 5151-5159Article in journal (Refereed) Published
Abstract [en]

PURPOSE.

To investigate the distribution of the intermediate filament (IF) proteins desmin, vimentin, and nestin in human extraocular muscles (EOMs). METHODS. Healthy adult EOM samples were serially sectioned (5 and 1 mu m) and processed for immunohistochemistry, with specific antibodies (Abs) against desmin, vimentin, and nestin and different myosin heavy chains (MyHCs), including the newly characterized Ab MYH7b against MyHC slow tonic. The distribution of desmin was also studied in EOMs at 16 to 18 weeks of gestation.

RESULTS.

Desmin was present in the vast majority of muscle fibers. Notably, muscle fibers that contained MyHC slow tonic were either unlabeled or very weakly labeled with three different Abs against desmin. These muscle fibers had normal cytoarchitecture and intact basement membrane. In fetal muscle, desmin was also absent or weak in myotubes containing MyHC slow tonic. Nestin was detected in a large proportion of muscle fibers in the orbital layer and to some extent also in the global layer, whereas no muscle fibers contained vimentin. Desmin and nestin were enriched at neuromuscular junctions, as in limb muscle. In contrast, some myotendinous junctions lacked desmin or nestin.

CONCLUSIONS.

The human EOMs differed significantly from the other muscles in the body with respect to their IF composition. Desmin, hitherto regarded as a ubiquitous muscle cytoskeletal protein, was absent or only present in trace amounts in a subset of normal muscle fibers in adult and fetal EOMs. Nestin, normally downregulated early in the postnatal period, was present in a high proportion of adult muscle fibers.

Keywords
extraocular muscle, intermediate filament, desmin, myosin heavy chain slow tonic, nestin
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:umu:diva-96638 (URN)10.1167/iovs.14-14316 (DOI)000343145500060 ()25028355 (PubMedID)
Available from: 2014-11-27 Created: 2014-11-24 Last updated: 2018-06-07Bibliographically approved
Österlund, C., Lindström, M., Thornell, L.-E. & Eriksson, P.-O. (2012). Remarkable heterogeneity in myosin heavy-chain composition of the human young masseter compared with young biceps brachii. Histochemistry and Cell Biology, 138(4), 669-682
Open this publication in new window or tab >>Remarkable heterogeneity in myosin heavy-chain composition of the human young masseter compared with young biceps brachii
2012 (English)In: Histochemistry and Cell Biology, ISSN 0948-6143, E-ISSN 1432-119X, Vol. 138, no 4, p. 669-682Article in journal (Refereed) Published
Abstract [en]

Adult human jaw muscles differ from limb and trunk muscles in enzyme-histochemical fibre type composition. Recently, we showed that the human masseter and biceps differ in fibre type pattern already at childhood. The present study explored the myosin heavy-chain (MyHC) expression in the young masseter and biceps muscles by means of gel electrophoresis (GE) and immuno-histochemical (IHC) techniques. Plasticity in MyHC expression during life was evaluated by comparing the results with the previously reported data for adult muscles. In young masseter, GE identified MyHC-I, MyHC-IIa MyHC-IIx and small proportions of MyHC-fetal and MyHC-alpha cardiac. Western blots confirmed the presence of MyHC-I, MyHC-IIa and MyHC-IIx. IHC revealed in the masseter six isomyosins, MyHC-I, MyHC-IIa, MyHC-IIx, MyHC-fetal, MyHC alpha-cardiac and a previously not reported isoform, termed MyHC-IIx'. The majority of the masseter fibres co-expressed two to four isoforms. In the young biceps, both GE and IHC identified MyHC-I, MyHC-IIa and MyHC-IIx. MyHC-I predominated in both muscles. Young masseter showed more slow and less-fast and fetal MyHC than the adult and elderly masseter. These results provide evidence that the young masseter muscle is unique in MyHC composition, expressing MyHC-alpha cardiac and MyHC-fetal isoforms as well as hitherto unrecognized potential spliced isoforms of MyHC-fetal and MyHC-IIx. Differences in masseter MyHC expression between young adult and elderly suggest a shift from childhood to adulthood towards more fast contractile properties. Differences between masseter and biceps are proposed to reflect diverse evolutionary and developmental origins and confirm that the masseter and biceps present separate allotypes of muscle.

National Category
Dentistry
Identifiers
urn:nbn:se:umu:diva-46738 (URN)10.1007/s00418-012-0985-5 (DOI)
Available from: 2011-09-13 Created: 2011-09-13 Last updated: 2018-06-08Bibliographically approved
Organisations

Search in DiVA

Show all publications