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Chen, Yang-Er
Publications (2 of 2) Show all publications
Chen, Y.-E., Yuan, S., Lezhneva, L., Meurer, J., Schwenkert, S., Mamedov, F. & Schröder, W. P. (2019). The Low Molecular Mass Photosystem II Protein PsbTn is Important for Light Acclimation. Plant Physiology, 179(4), 1739-1753
Open this publication in new window or tab >>The Low Molecular Mass Photosystem II Protein PsbTn is Important for Light Acclimation
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2019 (English)In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 179, no 4, p. 1739-1753Article in journal (Refereed) Published
Abstract [en]

Photosystem II (PSII) is a supramolecular complex containing over 30 protein subunits and a large set of cofactors including various pigments and quinones as well as Mn, Ca, Cl, and Fe ions. Eukaryotic PSII complexes contain many subunits not found in their bacterial counterparts, including the proteins PsbP, PsbQ, PsbS, and PsbW, as well as the highly homologous, low molecular mass subunits PsbTn1 and PsbTn2 whose function is currently unknown. To determine the function of PsbTn1 and PsbTn2, we generated single and double psbTn1 and psbTn2 knock-out mutants in Arabidopsis thaliana. Cross-linking and reciprocal co-immunoprecipitation experiments revealed that PsbTn is a lumenal PSII protein situated next to the cytochrome b559 subunit PsbE. The removal of the PsbTn proteins decreased the oxygen evolution rate and PSII core phosphorylation level but increased the susceptibility of PSII to photoinhibition and the production of reactive oxygen species. The assembly and stability of PSII were unaffected, indicating that the deficiencies of the psbTn1 psbTn2 double mutants are due to structural changes. Double mutants exhibited a higher rate of non-photochemical quenching of excited states than the wild type and single mutants, as well as slower state transition kinetics and a lower quantum yield of PSII when grown in the field. Based on these results, we propose that the main function of the PsbTn proteins is to enable PSII to acclimate to light shifts or intense illumination.

Place, publisher, year, edition, pages
Rockville: American Society of Plant Biologists, 2019
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-156389 (URN)10.1104/pp.18.01251 (DOI)000462993100045 ()30538167 (PubMedID)
Funder
Carl Tryggers foundation
Note

Short title: PsbTn protein regulates light adaptation

Available from: 2019-02-14 Created: 2019-02-14 Last updated: 2019-05-23Bibliographically approved
Zhang, Z.-W., Liu, T., Zeng, J., Chen, Y.-E., Yuan, M., Zhang, D.-W., . . . Yuan, S. (2015). Prediction of the next highly pathogenic avian influenza pandemic that can cause illness in humans. Infectious diseases of poverty, 4, Article ID 50.
Open this publication in new window or tab >>Prediction of the next highly pathogenic avian influenza pandemic that can cause illness in humans
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2015 (English)In: Infectious diseases of poverty, ISSN 2049-9957, Vol. 4, article id 50Article in journal (Refereed) Published
Abstract [en]

Background: In recent years, avian influenza viruses (AIVs) have seriously threatened human health. Questions such as: why do AIVs infect humans?, how quickly can an AIV become pandemic?, and which virus is the most dangerous? cannot be sufficiently answered using current bioinformatic studies. Method: Secondary structures and energies of representative 5'-untranslated region (UTR) of the HA gene were calculated. Then their secondary structures and energies were re-calculated after one or two nucleotide substitutions were introduced into the HA 5'-UTR. Phylogenetic trees on the basis of hemagglutinin (HA) and polymerase basic protein 2 (PB2) amino acid sequences and HA 5'-UTR nucleotide sequences were constructed. The connection between the energy and translation efficiency of 5'-UTR was confirmed by in vitro coupled transcription/translation assay. Results: The simplicity of the secondary structure of the 5'-UTR of the HA gene determines the overall virus replication rate and transmission potential. Point mutation assays show that the 5'-UTR sequences of the HA gene in the influenza subtypes H2N2, H3N2, and H7N9 have greater variation potentials than other virus subtypes. Conclusion: Some high-virulent strains of avian influenza might emerge in the next two to three years. The H2N2 subtype, once disappeared in humans, may stage a comeback. The current outbreak of H7N9 may become pandemic and cause even more deaths, if one or two bases are substituted in the 5'-UTR sequence of the HA gene.

Place, publisher, year, edition, pages
BioMed Central, 2015
Keywords
Avian influenza viruses, Hemagglutinin, Point mutation, Translation efficiency, 5'-untranslated region
National Category
Infectious Medicine
Identifiers
urn:nbn:se:umu:diva-114640 (URN)10.1186/s40249-015-0083-8 (DOI)000367174500001 ()26612517 (PubMedID)
Available from: 2016-02-02 Created: 2016-01-25 Last updated: 2018-06-07Bibliographically approved
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