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Robinson, Kathryn M
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Publications (10 of 14) Show all publications
Wang, J., Ding, J., Tan, B., Robinson, K. M., Michelson, I. H., Johansson, A., . . . Ingvarsson, P. K. (2018). A major locus controls local adaptation and adaptive life history variation in a perennial plant. Genome Biology, 19, Article ID 72.
Open this publication in new window or tab >>A major locus controls local adaptation and adaptive life history variation in a perennial plant
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2018 (English)In: Genome Biology, ISSN 1465-6906, E-ISSN 1474-760X, Vol. 19, article id 72Article in journal (Refereed) Published
Abstract [en]

Background: The initiation of growth cessation and dormancy represent critical life history trade offs between survival and growth and have important fitness effects in perennial plants Such adaptive life history traits often show strong local adaptation along environmental gradients but, despite then importance, the genetic architecture of these traits remains poorly understood.

Results: We integrate whole genome re sequencing with environmental and phenotypic data from common garden experiments to investigate the genomic basis of local adaptation across a latitudinal gradient in European aspen (Populus tremula). A single genomic region containing the PtFT2 gene mediates local adaptation in the timing of bud set and explains 65% of the observed genetic variation in bud set This locus is the likely target of a recent selective sweep that originated right before or during colonization of northern Scandinavia following the last glaciation Field and greenhouse experiments confirm that variation in PtFT2 gene expression affects the phenotypic variation in bud set that we observe in wild natural populations.

Conclusions: Our results reveal a major effect locus that determines the timing of bud set and that has facilitated rapid adaptation to shorter growing seasons and colder climates in European aspen. The discovery of a single locus explaining a substantial fraction of the variation in a key life-history trait is remarkable, given that such traits are generally considered to be highly polygenic. These findings provide a dramatic illustration of how loci of large effect for adaptive traits can arise and be maintained over large geographical scales in natural populations.

Place, publisher, year, edition, pages
BioMed Central, 2018
Keywords
Populus tremula, Local adaptation, Genomic basis, PtFT2, Adaptive traits, Selective sweep
National Category
Genetics
Identifiers
urn:nbn:se:umu:diva-150175 (URN)10.1186/s13059-018-1444-y (DOI)000434210500001 ()29866176 (PubMedID)
Funder
Swedish Research CouncilKnut and Alice Wallenberg Foundation
Note

Originally included in thesis in manuscript form.

Available from: 2018-07-18 Created: 2018-07-18 Last updated: 2018-08-14Bibliographically approved
Michelson, I. H., Ingvarsson, P. K., Robinson, K. M., Edlund, E., Eriksson, M. E., Nilsson, O. & Jansson, S. (2018). Autumn senescence in aspen is not triggered by day length. Physiologia Plantarum: An International Journal for Plant Biology, 162(1), 123-134
Open this publication in new window or tab >>Autumn senescence in aspen is not triggered by day length
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2018 (English)In: Physiologia Plantarum: An International Journal for Plant Biology, ISSN 0031-9317, E-ISSN 1399-3054, Vol. 162, no 1, p. 123-134Article in journal (Refereed) Published
Abstract [en]

Autumn senescence in mature aspens, grown under natural conditions, is initiated at almost the same date every year. The mechanism of such precise timing is not understood but we have previously shown that the signal must be derived from light. We studied variation in bud set and autumn senescence in a collection of 116 natural Eurasian aspen (Populus tremula) genotypes, from 12 populations in Sweden and planted in one northern and one southern common garden, to test the hypothesis that onset of autumn senescence is triggered by day length. We confirmed that, although bud set seemed to be triggered by a critical photoperiod/day length, other factors may influence it. The data on initiation of autumn senescence, on the other hand, were incompatible with the trigger being the day length per se, hence the trigger must be some other light-dependent factor.

Place, publisher, year, edition, pages
John Wiley & Sons, 2018
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-143636 (URN)10.1111/ppl.12593 (DOI)000418236000008 ()28591431 (PubMedID)
Available from: 2018-01-08 Created: 2018-01-08 Last updated: 2018-06-09Bibliographically approved
Albrectsen, B. R., Siddique, A. B., Decker, V. H., Unterseher, M. & Robinson, K. M. (2018). Both plant genotype and herbivory shape aspen endophyte communities. Oecologia, 187(2), 535-545
Open this publication in new window or tab >>Both plant genotype and herbivory shape aspen endophyte communities
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2018 (English)In: Oecologia, ISSN 0029-8549, E-ISSN 1432-1939, Vol. 187, no 2, p. 535-545Article in journal (Refereed) Published
Abstract [en]

Salicinoid phenolic glycosides are common defence substances in salicaceous trees and specialist leaf beetles use these compounds for their own defence against predators. Salicinoids vary qualitatively and qualitatively in aspen (Populus tremula) and this variation has a genetic basis. The foliar endophyte mycobiome is plentiful and we hypothesised that it is related to plant genotype, potentially mediated by salicinoid composition, and that interactions with the leaf beetle Chrysomela tremula may alter this relationship. We studied these three-way interactions in controlled greenhouse experiments. Endophytic fungi were isolated from sterilised leaf tissues with and without beetle damage, and from beetles. We confirmed that endophyte composition was influenced by host genotype. Beetle activity added generalist morphs to the mycobiome that overrode the initial host association. Yeast-like genera (Cryptococcus and Rhodotorula) were isolated only from beetle-damaged tissues and from beetles, whereas fast-growing filamentous fungi dominated beetle-free control plants. Competition experiments between filamentous fungi of plant origin and beetle-related yeasts suggested interaction of both stimulating and inhibiting modes of action amongst the fungi. As a result, we detected examples of amensalism, commensalism, parasitism and competition between the morphs tested, but we found no evidence of mutualism, and consequently no co-evolutionary relationship could be demonstrated, between yeasts carried by beetles, host genotype and associated filamentous morphs. Endophyte studies are method-dependent and high-throughput sequencing technology best define the fungal mycobiome, culturing however continues to be a cheap way to provide fundamental ecological insights and it is also required for experimental studies.

Place, publisher, year, edition, pages
Springer, 2018
Keywords
Herbivory, Arboreal endophytes, Salicinoid, Bipartite graphics, Competition
National Category
Botany Ecology
Identifiers
urn:nbn:se:umu:diva-150882 (URN)10.1007/s00442-018-4097-3 (DOI)000435111800016 ()29492690 (PubMedID)2-s2.0-85042590826 (Scopus ID)
Available from: 2018-08-31 Created: 2018-08-31 Last updated: 2018-08-31Bibliographically approved
Lin, Y.-C., Wang, J., Delhomme, N., Schiffthaler, B., Sundström, G., Zuccolo, A., . . . Street, N. R. (2018). Functional and evolutionary genomic inferences in Populus through genome and population sequencing of American and European aspen. Proceedings of the National Academy of Sciences of the United States of America, 115(46), E10970-E10978
Open this publication in new window or tab >>Functional and evolutionary genomic inferences in Populus through genome and population sequencing of American and European aspen
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2018 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 115, no 46, p. E10970-E10978Article in journal (Refereed) Published
Abstract [en]

The Populus genus is one of the major plant model systems, but genomic resources have thus far primarily been available for poplar species, and primarily Populus trichocarpa (Torr. & Gray), which was the first tree with a whole-genome assembly. To further advance evolutionary and functional genomic analyses in Populus, we produced genome assemblies and population genetics resources of two aspen species, Populus tremula L. and Populus tremuloides Michx. The two aspen species have distributions spanning the Northern Hemisphere, where they are keystone species supporting a wide variety of dependent communities and produce a diverse array of secondary metabolites. Our analyses show that the two aspens share a similar genome structure and a highly conserved gene content with P. trichocarpa but display substantially higher levels of heterozygosity. Based on population resequencing data, we observed widespread positive and negative selection acting on both coding and noncoding regions. Furthermore, patterns of genetic diversity and molecular evolution in aspen are influenced by a number of features, such as expression level, coexpression network connectivity, and regulatory variation. To maximize the community utility of these resources, we have integrated all presented data within the PopGenIE web resource (PopGenIE.org).

Place, publisher, year, edition, pages
NATL ACAD SCIENCES, 2018
Keywords
genome assembly, natural selection, coexpression, population genetics, Populus
National Category
Genetics
Identifiers
urn:nbn:se:umu:diva-154950 (URN)10.1073/pnas.1801437115 (DOI)000449934400020 ()30373829 (PubMedID)2-s2.0-85056516875 (Scopus ID)
Available from: 2019-01-07 Created: 2019-01-07 Last updated: 2019-01-07Bibliographically approved
Grimberg, Å., Lager, I., Street, N., Robinson, K. M., Marttila, S., Mähler, N., . . . Bhalerao, R. P. (2018). Storage lipid accumulation is controlled by photoperiodic signal acting via regulators of growth cessation and dormancy in hybrid aspen. New Phytologist, 219(2), 619-630
Open this publication in new window or tab >>Storage lipid accumulation is controlled by photoperiodic signal acting via regulators of growth cessation and dormancy in hybrid aspen
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2018 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 219, no 2, p. 619-630Article in journal (Refereed) Published
Abstract [en]

The signalling pathways that control seasonal modulation of carbon metabolism in perennial plants are poorly understood. Using genetic, metabolic and natural variation approaches, we identify factors mediating photoperiodic control of storage lipid accumulation in the model tree hybrid aspen (Populus tremula x tremuloides). We characterized lipid accumulation in transgenic hybrid aspen with impaired photoperiodic and hormonal responses. Genome-wide association mapping was performed in Swedish aspen (P.tremula) genotypes to determine genetic loci associated with genotype variation in lipid content. Our data show that the storage lipid triacylglycerol (TAG) accumulates in cambial meristem and pith rays of aspen in response to photoperiodic signal controlling growth cessation and dormancy induction. We show that photoperiodic control of TAG accumulation is mediated by the FLOWERING LOCUS T/CONSTANS module, which also controls the induction of growth cessation. Hormonal and chromatin remodelling pathways also contribute to TAG accumulation by photoperiodic signal. Natural variation exists in lipid accumulation that is controlled by input from multiple loci. Our data shed light on how the control of storage metabolism is temporally coordinated with growth cessation and dormancy by photoperiodic signal, and reveals that storage lipid accumulation between seeds and perennating organs of trees may involve distinct regulatory circuits.

Place, publisher, year, edition, pages
John Wiley & Sons, 2018
Keywords
abscisic acid (ABA), dormancy, flowering time, growth cessation, lipid biosynthesis, photoperiod, trees, triacylglycerol
National Category
Forest Science
Identifiers
urn:nbn:se:umu:diva-150755 (URN)10.1111/nph.15197 (DOI)000435948500017 ()29761498 (PubMedID)
Projects
Bio4Energy
Available from: 2018-08-27 Created: 2018-08-27 Last updated: 2019-09-06Bibliographically approved
Soolanayakanahally, R. Y., Guy, R. D., Street, N. R., Robinson, K. M., Silim, S. N., Albrectsen, B. R. & Jansson, S. (2015). Comparative physiology of allopatric Populus species: geographic clines in photosynthesis, height growth, and carbon isotope discrimination in common gardens. Frontiers in Plant Science, 6, Article ID 528.
Open this publication in new window or tab >>Comparative physiology of allopatric Populus species: geographic clines in photosynthesis, height growth, and carbon isotope discrimination in common gardens
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2015 (English)In: Frontiers in Plant Science, ISSN 1664-462X, E-ISSN 1664-462X, Vol. 6, article id 528Article in journal (Refereed) Published
Abstract [en]

Populus species with wide geographic ranges display strong adaptation to local environments. We studied the clinal patterns in phenology and ecophysiology in allopatric Populus species adapted to similar environments on different continents under common garden settings. As a result of climatic adaptation, both Populus tremula L. and Populus balsamifera L. display latitudinal clines in photosynthetic rates (A), whereby high-latitude trees of P. tremula had higher A compared to low-latitude trees and nearly so in P. balsamifera (p = 0.06). Stomatal conductance (g(S)) and chlorophyll content index (CCI) follow similar latitudinal trends. However, foliar nitrogen was positively correlated with latitude in P. balsamifera and negatively correlated in P. tremula. No significant trends in carbon isotope composition of the leaf tissue (delta C-13) were observed for both species; but, intrinsic water-use efficiency (WUEi) was negatively correlated with the latitude of origin in P. balsamifera. In spite of intrinsically higher A, high-latitude trees in both common gardens accomplished less height gain as a result of early bud set. Thus, shoot biomass was determined by height elongation duration (HED), which was well approximated by the number of days available for free growth between bud flush and bud set. We highlight the shortcoming of unreplicated outdoor common gardens for tree improvement and the crucial role of photoperiod in limiting height growth, further complicating interpretation of other secondary effects.

Place, publisher, year, edition, pages
Frontiers Media, 2015
Keywords
comparative physiology, poplar, common garden, latitude, bud set, photosynthesis, carbon isotope discrimination, water-use efficiency
National Category
Forest Science
Identifiers
urn:nbn:se:umu:diva-107300 (URN)10.3389/fpls.2015.00528 (DOI)000358589700001 ()26236324 (PubMedID)
Available from: 2015-08-24 Created: 2015-08-21 Last updated: 2018-06-07Bibliographically approved
Robinson, K. M., Hauzy, C., Loeuille, N. & Albrectsen, B. R. (2015). Relative impacts of environmental variation and evolutionary history on the nestedness and modularity of tree-herbivore networks. Ecology and Evolution, 5(14), 2898-2915
Open this publication in new window or tab >>Relative impacts of environmental variation and evolutionary history on the nestedness and modularity of tree-herbivore networks
2015 (English)In: Ecology and Evolution, ISSN 2045-7758, E-ISSN 2045-7758, Vol. 5, no 14, p. 2898-2915Article in journal (Refereed) Published
Abstract [en]

Nestedness and modularity are measures of ecological networks whose causative effects are little understood. We analyzed antagonistic plant-herbivore bipartite networks using common gardens in two contrasting environments comprised of aspen trees with differing evolutionary histories of defence against herbivores. These networks were tightly connected owing to a high level of specialization of arthropod herbivores that spend a large proportion of the life cycle on aspen. The gardens were separated by ten degrees of latitude with resultant differences in abiotic conditions. We evaluated network metrics and reported similar connectance between gardens but greater numbers of links per species in the northern common garden. Interaction matrices revealed clear nestedness, indicating subsetting of the bipartite interactions into specialist divisions, in both the environmental and evolutionary aspen groups, although nestedness values were only significant in the northern garden. Variation in plant vulnerability, measured as the frequency of herbivore specialization in the aspen population, was significantly partitioned by environment (common garden) but not by evolutionary origin of the aspens. Significant values of modularity were observed in all network matrices. Trait-matching indicated that growth traits, leaf morphology, and phenolic metabolites affected modular structure in both the garden and evolutionary groups, whereas extra-floral nectaries had little influence. Further examination of module configuration revealed that plant vulnerability explained considerable variance in web structure. The contrasting conditions between the two gardens resulted in bottom-up effects of the environment, which most strongly influenced the overall network architecture, however, the aspen groups with dissimilar evolutionary history also showed contrasting degrees of nestedness and modularity. Our research therefore shows that, while evolution does affect the structure of aspen-herbivore bipartite networks, the role of environmental variations is a dominant constraint.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2015
Keywords
antagonism, arthropod, aspen, bipartite networks, degree of specialization, modularity, nestedness, ophic strength
National Category
Ecology
Identifiers
urn:nbn:se:umu:diva-107303 (URN)10.1002/ece3.1559 (DOI)000358452700013 ()
Available from: 2015-08-24 Created: 2015-08-21 Last updated: 2018-06-07Bibliographically approved
Robinson, K., Delhomme, N., Mahler, N., Schiffthaler, B., Önskog, J., Albrectsen, B., . . . Street, N. (2014). Populus tremula (European aspen) shows no evidence of sexual dimorphism. BMC Plant Biology, 14, 276
Open this publication in new window or tab >>Populus tremula (European aspen) shows no evidence of sexual dimorphism
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2014 (English)In: BMC Plant Biology, ISSN 1471-2229, E-ISSN 1471-2229, Vol. 14, p. 276-Article in journal (Refereed) Published
Abstract [en]

Background:

Evolutionary theory suggests that males and females may evolve sexually dimorphic phenotypic and biochemical traits concordant with each sex having different optimal strategies of resource investment to maximise reproductive success and fitness. Such sexual dimorphism would result in sex biased gene expression patterns in non-floral organs for autosomal genes associated with the control and development of such phenotypic traits.

Results:

We examined morphological, biochemical and herbivory traits to test for sexually dimorphic resource allocation strategies within collections of sexually mature and immature Populus tremula (European aspen) trees. In addition we profiled gene expression in mature leaves of sexually mature wild trees using whole-genome oligonucleotide microarrays and RNA-Sequencing.

Conclusions:

We found no evidence of sexual dimorphism or differential resource investment strategies between males and females in either sexually immature or mature trees. Similarly, single-gene differential expression and machine learning approaches revealed no evidence of large-scale sex biased gene expression. However, two significantly differentially expressed genes were identified from the RNA-Seq data, one of which is a robust diagnostic marker of sex in P. tremula.

Keywords
Sexual dimorphism, RNA-Sequencing, transcriptomics, Populus tremula, dioecious
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-96608 (URN)10.1186/s12870-014-0276-5 (DOI)000343265100001 ()
Available from: 2014-11-27 Created: 2014-11-24 Last updated: 2018-06-07Bibliographically approved
Bernhardsson, C., Robinson, K. M., Abreu, I. N., Jansson, S., Albrectsen, B. R. & Ingvarsson, P. K. (2013). Geographic structure in metabolome and herbivore community co-occurs with genetic structure in plant defence genes. Ecology Letters, 16(6), 791-798
Open this publication in new window or tab >>Geographic structure in metabolome and herbivore community co-occurs with genetic structure in plant defence genes
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2013 (English)In: Ecology Letters, ISSN 1461-023X, E-ISSN 1461-0248, Vol. 16, no 6, p. 791-798Article in journal (Refereed) Published
Abstract [en]

Plantherbivore interactions vary across the landscape and have been hypothesised to promote local adaption in plants to the prevailing herbivore regime. Herbivores that feed on European aspen (Populus tremula) change across regional scales and selection on host defence genes may thus change at comparable scales. We have previously observed strong population differentiation in a set of inducible defence genes in Swedish P. tremula. Here, we study the geographic patterns of abundance and diversity of herbivorous insects, the untargeted metabolome of the foliage and genetic variation in a set of wound-induced genes and show that the geographic structure co-occurs in all three data sets. In response to this structure, we observe local maladaptation of herbivores, with fewer herbivores on local trees than on trees originated from more distant localities. Finally, we also identify 28 significant associations between single nucleotide polymorphisms SNPs from defence genes and a number of the herbivore traits and metabolic profiles.

Place, publisher, year, edition, pages
Hoboken: Wiley-Blackwell, 2013
National Category
Ecology Earth and Related Environmental Sciences
Identifiers
urn:nbn:se:umu:diva-73567 (URN)10.1111/ele.12114 (DOI)000318996400009 ()
Available from: 2013-06-25 Created: 2013-06-25 Last updated: 2018-06-08Bibliographically approved
Robinson, K. M., Ingvarsson, P. K., Jansson, S. & Albrectsen, B. R. (2012). Genetic Variation in Functional Traits Influences Arthropod Community Composition in Aspen (Populus tremula L.). PLoS ONE, 7(5), e37679
Open this publication in new window or tab >>Genetic Variation in Functional Traits Influences Arthropod Community Composition in Aspen (Populus tremula L.)
2012 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 7, no 5, p. e37679-Article in journal (Refereed) Published
Abstract [en]

We conducted a study of natural variation in functional leaf traits and herbivory in 116 clones of European aspen, Populus tremula L., the Swedish Aspen (SwAsp) collection, originating from ten degrees of latitude across Sweden and grown in a common garden. In surveys of phytophagous arthropods over two years, we found the aspen canopy supports nearly 100 morphospecies. We identified significant broad-sense heritability of plant functional traits, basic plant defence chemistry, and arthropod community traits. The majority of arthropods were specialists, those coevolved with P. tremula to tolerate and even utilize leaf defence compounds. Arthropod abundance and richness were more closely related to plant growth rates than general chemical defences and relationships were identified between the arthropod community and stem growth, leaf and petiole morphology, anthocyanins, and condensed tannins. Heritable genetic variation in plant traits in young aspen was found to structure arthropod community; however no single trait drives the preferences of arthropod folivores among young aspen genotypes. The influence of natural variation in plant traits on the arthropod community indicates the importance of maintaining genetic variation in wild trees as keystone species for biodiversity. It further suggests that aspen can be a resource for the study of mechanisms of natural resistance to herbivores.

National Category
Ecology
Identifiers
urn:nbn:se:umu:diva-57443 (URN)10.1371/journal.pone.0037679 (DOI)000305342300080 ()
Available from: 2012-07-23 Created: 2012-07-23 Last updated: 2018-06-08Bibliographically approved
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