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Guinea Diaz, Manuel
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Publications (3 of 3) Show all publications
Guinea Diaz, M., Hernandez-Verdeja, T., Kremnev, D., Crawford, T., Dubreuil, C. & Strand, Å. (2018). Redox regulation of PEP activity during seedling establishment in Arabidopsis thaliana. Nature Communications, 9, Article ID 50.
Open this publication in new window or tab >>Redox regulation of PEP activity during seedling establishment in Arabidopsis thaliana
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2018 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 9, article id 50Article in journal (Refereed) Published
Abstract [en]

Activation of the plastid-encoded RNA polymerase is tightly controlled and involves a network of phosphorylation and, as yet unidentified, thiol-mediated events. Here, we characterize PLASTID REDOX INSENSITIVE2, a redox-regulated protein required for full PEP-driven transcription. PRIN2 dimers can be reduced into the active monomeric form by thioredoxins through reduction of a disulfide bond. Exposure to light increases the ratio between the monomeric and dimeric forms of PRIN2. Complementation of prin2-2 with different PRIN2 protein variants demonstrates that the monomer is required for light-activated PEP-dependent transcription and that expression of the nuclear-encoded photosynthesis genes is linked to the activity of PEP. Activation of PEP during chloroplast development likely is the source of a retrograde signal that promotes nuclear LHCB expression. Thus, regulation of PRIN2 is the thiol-mediated mechanism required for full PEP activity, with PRIN2 monomerization via reduction by TRXs providing a mechanistic link between photosynthetic electron transport and activation of photosynthetic gene expression.

National Category
Other Biological Topics
Identifiers
urn:nbn:se:umu:diva-144086 (URN)10.1038/s41467-017-02468-2 (DOI)000419308300001 ()29298981 (PubMedID)
Available from: 2018-01-29 Created: 2018-01-29 Last updated: 2018-06-09Bibliographically approved
Blanco, N. E., Guinea-Diaz, M., Whelan, J. & Strand, Å. (2014). Interaction between plastid and mitochondrial retrograde signalling pathways during changes to plastid redox status. Philosophical Transactions of the Royal Society of London. Biological Sciences, 369(1640), Article ID 20130231.
Open this publication in new window or tab >>Interaction between plastid and mitochondrial retrograde signalling pathways during changes to plastid redox status
2014 (English)In: Philosophical Transactions of the Royal Society of London. Biological Sciences, ISSN 0962-8436, E-ISSN 1471-2970, Vol. 369, no 1640, article id 20130231Article in journal (Refereed) Published
Abstract [en]

Mitochondria and chloroplasts depend upon each other; photosynthesis provides substrates for mitochondrial respiration and mitochondrial metabolism is essential for sustaining photosynthetic carbon assimilation. In addition, mitochondrial respiration protects photosynthesis against photoinhibition by dissipating excess redox equivalents from the chloroplasts. Genetic defects in mitochondrial function result in an excessive reduction and energization of the chloroplast. Thus, it is clear that the activities of mitochondria and plastids need to be coordinated, but the manner by which the organelles communicate to coordinate their activities is unknown. The regulator of alternative oxidase (rao1) mutant was isolated as a mutant unable to induce AOX1a expression in response to the inhibitor of the mitochondrial cytochrome c reductase (complex III), antimycin A. RAO1 encodes the nuclear localized cyclin-dependent kinase E1 (CDKE1). Interestingly, the rao1 mutant demonstrates a genome uncoupled phenotype also in response to redox changes in the photosynthetic electron transport chain. Thus, CDKE1 was shown to regulate both LIGHT HARVESTING COMPLEX B (LHCB) and ALTERNATIVE OXIDASE 1 (AOX1a) expression in response to retrograde signals. Our results suggest that CDKE1 is a central nuclear component integrating mitochondrial and plastid retrograde signals and plays a role in regulating energy metabolism during the response to stress.

Place, publisher, year, edition, pages
Royal Society Publishing, 2014
Keywords
redox, retrograde, chloroplast, mitochondria, kinase
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-87866 (URN)10.1098/rstb.2013.0231 (DOI)000332468400011 ()
Note

Special Issue: Changing the light environment: chloroplast signalling and response mechanisms

Available from: 2014-04-14 Created: 2014-04-14 Last updated: 2018-06-08Bibliographically approved
Kremnev, D., Guinea Diaz, M., Dubreuil, C. & Strand, Å. Redox regulation of PEP activity during seedling development in Arabidopsis.
Open this publication in new window or tab >>Redox regulation of PEP activity during seedling development in Arabidopsis
(English)Manuscript (preprint) (Other academic)
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-96176 (URN)
Available from: 2014-11-11 Created: 2014-11-11 Last updated: 2018-06-07Bibliographically approved
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