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Juto, Per
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Publications (5 of 5) Show all publications
Evander, M., Eriksson, I., Pettersson, L., Juto, P., Ahlm, C., Olsson, G. E., . . . Allard, A. (2007). Puumala hantavirus viremia diagnosed by real-time reverse transcriptase PCR using samples from patients with hemorrhagic fever and renal syndrome.. Journal of Clinical Microbiology, 45(8), 2491-7
Open this publication in new window or tab >>Puumala hantavirus viremia diagnosed by real-time reverse transcriptase PCR using samples from patients with hemorrhagic fever and renal syndrome.
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2007 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 45, no 8, p. 2491-7Article in journal (Refereed) Published
Abstract [en]

Puumala virus (PUUV) is the endemic hantavirus in northern Sweden and causes nephropathia epidemica (NE), a milder form of hemorrhagic fever with renal syndrome. There is a need for fast and reliable diagnostics to differentiate the disease from other infections. By aligning virus RNA sequences isolated from 11 different bank voles and one human patient, we designed a real-time reverse transcriptase (RT) PCR method for detection of PUUV RNA. The real-time RT-PCR assay showed linearity from 20 to 2 x 10(6) virus copies with a correlation coefficient above 0.98 to 0.99 for all experiments. The detection threshold for PUUV cDNA was two copies per reaction. A two-step qualitative RT-PCR to detect PUUV RNA showed 100% concordance with the real-time RT-PCR assay. PUUV RNA viremia was detected in 33 of 34 PUUV immunoglobulin M (IgM)-positive patients with typical clinical NE disease from the region of endemicity. One PUUV IgM-negative sample had PUUV RNA, and 4 days later, the patient was IgM positive. Of samples with indeterminate IgM, 43% were PUUV RNA positive. The kinetics of antibody titers and PUUV viremia were studied, and five of six NE patients displayed a decrease in PUUV viremia a few days after disease outbreak coupled with an increase in PUUV IgM and IgG. In one patient with continuously high PUUV RNA levels but low IgM and no IgG response, the infection was lethal. These findings demonstrated that real-time RT-PCR is a useful method for diagnosis of PUUV viremia and for detecting PUUV RNA at early time points, before the appearance of IgM antibodies.

Identifiers
urn:nbn:se:umu:diva-20652 (URN)10.1128/JCM.01902-06 (DOI)17537944 (PubMedID)
Available from: 2009-03-24 Created: 2009-03-24 Last updated: 2018-06-09
Sundström, P., Juto, P., Wadell, G., Hallmans, G., Svenningsson, A., Nyström, L., . . . Forsgren, L. (2004). An altered immune response to Epstein-Barr virus in multiple sclerosis: a prospective study.. Neurology, 62(12), 2277-82
Open this publication in new window or tab >>An altered immune response to Epstein-Barr virus in multiple sclerosis: a prospective study.
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2004 (English)In: Neurology, ISSN 1526-632X, Vol. 62, no 12, p. 2277-82Article in journal (Refereed) Published
Keywords
Adolescent, Adult, Aged, Antigens; Viral/immunology, Capsid Proteins/immunology, Epstein-Barr Virus Infections/*immunology, Epstein-Barr Virus Nuclear Antigens/immunology, Female, Herpesvirus 4; Human/*immunology, Humans, Immunoglobulin G/immunology, Male, Middle Aged, Multiple Sclerosis/*immunology/*virology, Prospective Studies, Retrospective Studies
Identifiers
urn:nbn:se:umu:diva-13772 (URN)15210894 (PubMedID)
Available from: 2007-11-23 Created: 2007-11-23 Last updated: 2018-06-09Bibliographically approved
Olsson, G. E., Ahlm, C., Elgh, F., Verlemyr, A.-C., White, N., Juto, P. & Palo, R. T. (2003). Hantavirus antibody occurrence in bank voles (Clethrionomys glareolus) during a vole population cycle. Journal of Wildlife Diseases, 39(2), 299-305
Open this publication in new window or tab >>Hantavirus antibody occurrence in bank voles (Clethrionomys glareolus) during a vole population cycle
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2003 (English)In: Journal of Wildlife Diseases, ISSN 0090-3558, E-ISSN 1943-3700, Vol. 39, no 2, p. 299-305Article in journal (Refereed) Published
Abstract [en]

Puumala virus, genus Hantavirus, is the etiologic agent of nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome. The bank vole (Clethrionomys glareolus) is the natural reservoir species of this hantavirus. We initiated sampling of bank voles at sites of recently identified human nephropathia epidemica cases and paired control sites in the fall of 1995 in coastal areas of northern Sweden. Sites were trapped annually in spring and fall until 1999. Prevalence of antibody to Puumala virus was similar among local bank vole populations in the two types of sites over time. During peak years, however, the absolute number of bank voles was higher in case sites than control sites. Consequently, the likelihood of Puumala virus exposure was increased at case sites during population highs. This would imply that the risk of Puumala virus exposure to conspecifics and humans is habitat and site dependent with a temporal component.

Place, publisher, year, edition, pages
Wildlife Disease Association, 2003
Keywords
Bank vole, Clethrionomys glareolus, habitat, hantavirus, nephropathia epidemica, population dynamics, Puumala virus, rodents
Identifiers
urn:nbn:se:umu:diva-41884 (URN)12910756 (PubMedID)
Available from: 2011-04-01 Created: 2011-04-01 Last updated: 2018-06-08Bibliographically approved
Olsson, G. E., Dalerum, F., Hörnfeldt, B., Elgh, F., Palo, T. R., Juto, P. & Ahlm, C. (2003). Human hantavirus infections, Sweden.. Emerging Infectious Diseases, 9(11), 1395-401
Open this publication in new window or tab >>Human hantavirus infections, Sweden.
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2003 (English)In: Emerging Infectious Diseases, ISSN 1080-6040, E-ISSN 1080-6059, Vol. 9, no 11, p. 1395-401Article in journal (Refereed) Published
Abstract [en]

The prevalent human hantavirus disease in Sweden is nephropathia epidemica, which is caused by Puumala virus and shed by infected bank voles (Clethrionomys glareolus). To evaluate temporal and spatial patterns of this disease, we studied 2,468 reported cases from a highly disease-endemic region in northern Sweden. We found that, in particular, middle-aged men living in rural dwellings near coastal areas were overrepresented. The case-patients were most often infected in late autumn, when engaged in activities near or within manmade rodent refuges. Of 862 case-patients confident about the site of virus exposure, 50% were concentrated within 5% of the study area. The incidence of nephropathia epidemica was significantly correlated with bank vole numbers within monitored rodent populations in part of the region. Understanding this relationship may help forestall future human hantavirus outbreaks.

Identifiers
urn:nbn:se:umu:diva-41881 (URN)14718081 (PubMedID)
Available from: 2011-04-01 Created: 2011-04-01 Last updated: 2018-06-08
Mincheva-Nilsson, L., Hammarström, M.-L., Juto, P. & Hammarström, S. (1990). Human milk contains proteins that stimulate and suppress T lymphocyte proliferation.. Clinical and Experimental Immunology, 79(3), 463-9
Open this publication in new window or tab >>Human milk contains proteins that stimulate and suppress T lymphocyte proliferation.
1990 (English)In: Clinical and Experimental Immunology, ISSN 0009-9104, E-ISSN 1365-2249, Vol. 79, no 3, p. 463-9Article in journal (Refereed) Published
Abstract [en]

The modulatory effect of human milk proteins from colostrum and late milk on the proliferative response of human T lymphocytes activated by mitogens (OKT3 and leucoagglutinin from Phaseolus vulgaris) and alloantigens was studied. High concentrations (10-100 micrograms/ml) of crude colostral milk proteins had an inhibitory effect on T cell growth while low concentrations (0.1-1 microgram/ml) enhanced T cells growth. In contrast, proteins from late milk did not inhibit T lymphocyte proliferation while the enhancing effect was retained. Colostrum was fractionated by ammonium sulphate precipitation and gel filtration on sepharose 6B. The inhibitory activity was recovered in a protein fraction containing lactoferrin as its major component. Lactoferrin was, however, not responsible for the observed inhibition. On the contrary, lactoferrin in most cases augmented the proliferative response induced by polyclonal activators. The inhibitory activity was found to bind concanavalin A-sepharose suggesting an association with glycoprotein. Inhibitory fractions contained glycoproteins of the following molecular sizes 26, 74/76 (doublet), 84, 145 and 160 kD under reducing conditions. The inhibitory effect appeared to be lymphocyte specific since the active fraction did not inhibit the growth of tissue culture cells (HeLa cells and human fibroblasts) or bacteria. Furthermore, the fraction was not toxic for lymphocytes. The inhibitory colostrum factor may prevent the newborn from overreacting immunologically against the environmental antigens encountered at birth.

Keywords
inflammatory-bowel-disease; soluble fas ligand; t-cells; ulcerative-colitis; celiac-disease; crohns-disease; regional specialization; immune-system; human gut; expression
National Category
Immunology
Identifiers
urn:nbn:se:umu:diva-67888 (URN)2317950 (PubMedID)
Available from: 2013-04-07 Created: 2013-04-07 Last updated: 2018-06-08
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