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Ransjö, Maria
Publications (10 of 13) Show all publications
Mladenovic, Z., Sahlin-Platt, A., Andersson, B., Johansson, A., Björn, E. & Ransjö, M. (2013). In vitro study of the biological interface of Bio-Oss: implications of the experimental setup. Clinical Oral Implants Research, 24(3), 329-335
Open this publication in new window or tab >>In vitro study of the biological interface of Bio-Oss: implications of the experimental setup
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2013 (English)In: Clinical Oral Implants Research, ISSN 0905-7161, E-ISSN 1600-0501, Vol. 24, no 3, p. 329-335Article in journal (Refereed) Published
Abstract [en]

Objectives To systematically investigate the biological interface of Bio-Oss by analysing dissolution–precipitation behaviour and osteogenic responses using in vitro experimental systems.

Material and methods Different concentrations (1–100 mg/ml) of Bio-Oss were incubated in cell culture medium for 24 h before elemental concentrations for calcium, phosphorus and silicon in the medium were analysed with inductive coupled plasma-optical emission spectroscopy. Radioactive calcium-45 isotope labelling technique was used to study possible precipitation of calcium on the Bio-Oss particle. Biological interface of Bio-Oss was studied in osteogenic experiments using mineralization medium and three different sources of cells (primary mouse bone marrow stromal cells, primary rat calvarial cells and MC3T3-E1 mouse pre-osteoblast cell line). Cells were fixed and stained with Toulidine blue, von Kossa or Alizarin Red staining for confirmation of extracellular matrix mineralization.

Results Elemental analysis of the cell culture medium demonstrated a significant decrease of calcium and phosphorus and a dose-dependent release of silicon to the medium after incubation with Bio-Oss. A significant decrease of calcium and phosphorus in the medium occurred even at low concentrations of Bio-Oss. Uptake of calcium on the Bio-Oss particle was confirmed with radioactive calcium-45 isotope labelling technique. In osteogenic experiments with Bio-Oss (<1 mg/ml), matrix mineralization around the Bio-Oss particles were demonstrated in all three cell types with von Kossa and Alizarin Red staining.

Conclusion Dissolution–precipitation reactions occur at the surface of Bio-Oss, and osteogenic responses are seen at the biological interface. The concentration of Bio-Oss is a key factor for the experimental in vitro results, and may also have implications for the clinic.

Place, publisher, year, edition, pages
John Wiley & Sons, 2013
Keywords
Bio-Oss, bone graft, cell culture, ICP-OES, interface, mineralization, xenograft
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-47367 (URN)10.1111/j.1600-0501.2011.02334.x (DOI)000314656500013 ()22092546 (PubMedID)
Note

Article first published online: 1 NOV 2011

Available from: 2011-09-20 Created: 2011-09-20 Last updated: 2018-06-08Bibliographically approved
Shchukarev, A., Mladenovic, Z. & Ransjö, M. (2012). Surface characterization of bone graft substitute materials conditioned in cell culture medium. 2. Protein adsorption. Surface and Interface Analysis, 44(8), 919-923
Open this publication in new window or tab >>Surface characterization of bone graft substitute materials conditioned in cell culture medium. 2. Protein adsorption
2012 (English)In: Surface and Interface Analysis, ISSN 0142-2421, E-ISSN 1096-9918, Vol. 44, no 8, p. 919-923Article in journal (Refereed) Published
Abstract [en]

Three bone graft substitute materials (Bioglass 45S5, Bio-Oss (R) and Algipore (R)) were conditioned in a-minimum essential medium (alpha-MEM), with the addition of 10% fetal bovine serum (FBS), for 1 and 7?days. The chemistry of their solid-solution interface was monitored by X-ray photoelectron spectroscopy, using fast-frozen sample technique, and compared to that reported for original alpha-MEM. FBS added to the biological medium causes significant changes in the interface after only 1day of conditioning. Interfacial chemical composition and N 1s spectra show immediate adsorption of proteins at the surface of all three biomaterials, independent of their surface charge and chemical composition. However, the atomic ratio C/N and the C 1s spectra indicate a different orientation of adsorbed serum proteins, which is dependent on the particle's surface charge. Moreover, the adsorption of serum proteins at the surface of Bio-Oss causes a charge reversal at the interface, as evidenced by the change in the atomic ratio of Na/Cl. In addition to the particle's surface charge, the formation of the protein interfacial layer at the surface of the biomaterial seems to be the second major phenomenon important for subsequent cell recognition and the initiation of biomineralization. Copyright (c) 2012 John Wiley & Sons, Ltd.

Place, publisher, year, edition, pages
Hoboken, NJ: Wiley-Blackwell, 2012
Keywords
cryogenic XPS, solid-liquid interface, bone graft substitute, biomaterial, Bio-Oss, Algipore, Bioglass 45S5, serum, protein adsorption
National Category
Chemical Sciences
Identifiers
urn:nbn:se:umu:diva-60088 (URN)10.1002/sia.5012 (DOI)000306662600009 ()
Available from: 2012-10-05 Created: 2012-10-01 Last updated: 2018-06-08Bibliographically approved
Shchukarev, A., Ransjö, M. & Mladenović, Ž. (2011). To build or not to build: the interface of bone graft substitute materials in biological media from the view point of the cells. In: Rosario Pignatello (Ed.), Biomaterials science and engineering (pp. 287-308). Published by InTech
Open this publication in new window or tab >>To build or not to build: the interface of bone graft substitute materials in biological media from the view point of the cells
2011 (English)In: Biomaterials science and engineering / [ed] Rosario Pignatello, Published by InTech , 2011, p. 287-308Chapter in book (Refereed)
Abstract [en]

These contribution books collect reviews and original articles from eminent experts working in the interdisciplinary arena of biomaterial development and use. From their direct and recent experience, the readers can achieve a wide vision on the new and ongoing potentials of different synthetic and engineered biomaterials. Contributions were not selected based on a direct market or clinical interest, than on results coming from very fundamental studies which have been mainly gathered for this book. This fact will also allow to gain a more general view of what and how the various biomaterials can do and work for, along with the methodologies necessary to design, develop and characterize them, without the restrictions necessarily imposed by industrial or profit concerns. The book collects 22 chapters related to recent researches on new materials, particularly dealing with their potential and different applications in biomedicine and clinics: from tissue engineering to polymeric scaffolds, from bone mimetic products to prostheses, up to strategies to manage their interaction with living cells.

Place, publisher, year, edition, pages
Published by InTech, 2011
National Category
Chemical Sciences
Identifiers
urn:nbn:se:umu:diva-46990 (URN)978-953-307-609-6 (ISBN)
Note
First published August, 2011. Printed in Croatia.Available from: 2011-09-19 Created: 2011-09-19 Last updated: 2018-06-08Bibliographically approved
Mladenovic, Z., Sahlin-Platt, A., Bengtsson, Å., Ransjö, M. & Shchukarev, A. (2010). Surface characterization of bone graft substitute materials conditioned in cell culture medium. Surface and Interface Analysis, 42(6-7), 452-456
Open this publication in new window or tab >>Surface characterization of bone graft substitute materials conditioned in cell culture medium
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2010 (English)In: Surface and Interface Analysis, ISSN 0142-2421, E-ISSN 1096-9918, Vol. 42, no 6-7, p. 452-456Article in journal (Refereed) Published
Abstract [en]

Biomaterials are widely used in clinical practice as bone graft substitutes for treating patients with bone defects. A molecular level understanding of the chemical processes at the interface between the biomaterial and the biological environment is crucial to succeed in tissue regeneration and to predict the treatment outcome. In this study, we used three different bone graft substitute materials (BioGlass 45S5—synthetic, Bio-Oss—bovine derived and Algipore—derived from algae) which were incubated in an α-minimum essential medium (α-MEM) during 1, 3 and 7 days. Initial surface composition of the biomaterials and the chemistry of their solid–solution interface were monitored by XPS with a fast-frozen samples technique. The XPS analysis showed that the equilibrium at the solid-solution interface is reached within 24 h. The Na/Cl atomic ratio at equilibrium indicates a negatively charged surface for Bio-Oss. In contrast, the other two materials gained a positive surface charge, which resulted in pronounced adsorption of amino acids at the interface from the medium. The surface chemical reconstruction and charge generation mechanism responsible for this effect are discussed with regard to bulk composition of the materials and possible proliferation and differentiation cell patterns that could be expected at the interface. Copyright © 2010 John Wiley & Sons, Ltd.

Place, publisher, year, edition, pages
John Wiley & Sons, Ltd, 2010
Keywords
XPS, solid–liquid interface, bone graft substitute, biomaterial, Bio-Oss, Algipore, Bioglass 45S5
National Category
Dentistry
Identifiers
urn:nbn:se:umu:diva-35478 (URN)10.1002/sia.3337 (DOI)000281149700004 ()
Available from: 2010-08-19 Created: 2010-08-19 Last updated: 2018-06-08Bibliographically approved
Sahlin-Platt, A., Örtengren, U., Mladenovic, Z. & Ransjö, M. (2008). Effects of Dyract AP and released ionic products on periodontal ligament cells and bone marrow cultures. Dental Materials, 24(12), 1623-1630
Open this publication in new window or tab >>Effects of Dyract AP and released ionic products on periodontal ligament cells and bone marrow cultures
2008 (English)In: Dental Materials, ISSN 0109-5641, E-ISSN 1879-0097, Vol. 24, no 12, p. 1623-1630Article in journal (Refereed) Published
Abstract [en]

OBJECTIVES: The aim of this work was to investigate the release of inorganic ionic products from specimens of the polyacid-modified composite resin Dyract AP (DAP) and furthermore, to analyze the biological effect of DAP and the medium extract in human periodontal ligament (PDL) cells and mouse bone marrow cell (BMC) cultures.

METHODS: Ion release from DAP specimens immersed in cell culture medium was analyzed with inductively coupled plasma optical emission spectroscopy (ICP-OES). Cells were cultured with either DAP specimens or with DAP media extract and effects on cell proliferation, osteoblastic gene expression and mineralization capacity were analyzed with direct-contact tests, neutral red (NR) uptake, quantitative real-time PCR and a bone nodule formation assay.

RESULTS: ICP-OES analysis of DAP extract demonstrated a significant increase in fluoride, strontium and silica. PDL cells demonstrated normal growth pattern in the direct-contact tests with the material. DAP extracts produced a dose-dependent stimulation of cell proliferation and concomitant inhibition of osteoblast specific markers and nodule formation.

SIGNIFICANCE: The compomer may have possible bioactive properties due to ions leaching out from the filler component.

Keywords
Dyract AP; Mouse bone marrow cells; Compomer, Biocompability, Ion release, Gene expression, Human PDL cells
National Category
Dentistry
Identifiers
urn:nbn:se:umu:diva-20783 (URN)10.1016/j.dental.2008.03.024 (DOI)18471872 (PubMedID)
Available from: 2009-03-25 Created: 2009-03-25 Last updated: 2018-06-09Bibliographically approved
Lundgren, S., Sennerby, L., Cricchio, G., Salata, L., Palma, V., Lundqvist, C. & Ransjö, M. (2008). Rekonstruktiv käkkirurgi: Behandling av den atrofiska posteriora maxillan hos partiellt betandade patienter. Tandläkartidningen, 100(5), 70-71
Open this publication in new window or tab >>Rekonstruktiv käkkirurgi: Behandling av den atrofiska posteriora maxillan hos partiellt betandade patienter
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2008 (Swedish)In: Tandläkartidningen, ISSN 0039-6982, Vol. 100, no 5, p. 70-71Article in journal (Other (popular science, discussion, etc.)) Published
National Category
Dentistry
Identifiers
urn:nbn:se:umu:diva-34572 (URN)
Available from: 2010-06-08 Created: 2010-06-08 Last updated: 2018-06-08Bibliographically approved
Ransjö, M. & Lundgren, S. (2006). Det växer fast - om implantat och benbildning.: På bettet hela livet, om  odontologisk vetenskap i Umeå. pp. In: Medicinska fakulteten vid Umeå universitet (Ed.), På bettet hela livet, om  odontologisk vetenskap i Umeå.: En bok från Forskningens dag 2006, Medicinska fakulteten, Umeå universitet. Författare Svante Twetman (pp. 67-82). Umeå: Umeå universitet
Open this publication in new window or tab >>Det växer fast - om implantat och benbildning.: På bettet hela livet, om  odontologisk vetenskap i Umeå. pp
2006 (Swedish)In: På bettet hela livet, om  odontologisk vetenskap i Umeå.: En bok från Forskningens dag 2006, Medicinska fakulteten, Umeå universitet. Författare Svante Twetman / [ed] Medicinska fakulteten vid Umeå universitet, Umeå: Umeå universitet , 2006, p. 67-82Chapter in book (Other (popular science, discussion, etc.))
Place, publisher, year, edition, pages
Umeå: Umeå universitet, 2006
Keywords
implantat, benbildning
National Category
Dentistry
Research subject
Odontology
Identifiers
urn:nbn:se:umu:diva-26087 (URN)
Projects
På bettet hela livet, om odontologisk vetenskap i Umeå
Available from: 2009-09-23 Created: 2009-09-23 Last updated: 2018-06-08Bibliographically approved
Matemba, S., Lie, A. & Ransjö, M. (2006). Regulation of osteoclastogenesis by gap junction communication. Journal of cellular biochemistry, 99(2), 528-37
Open this publication in new window or tab >>Regulation of osteoclastogenesis by gap junction communication
2006 (English)In: Journal of cellular biochemistry, Vol. 99, no 2, p. 528-37Article in journal (Refereed) Published
Abstract [en]

Receptor activator of NF-kappaB ligand (RANKL) is crucial in osteoclastogenesis but signaling events involved in osteoclast differentiation are far from complete and other signals may play a role in osteoclastogenesis. A more direct pathway for cellular crosstalk is provided by gap junction intercellular channel, which allows adjacent cells to exchange second messengers, ions, and cellular metabolites. Here we have investigated the role of gap junction communication in osteoclastogenesis in mouse bone marrow cultures. Immunoreactive sites for the gap junction protein connexin 43 (Cx43) were detected in the marrow stromal cells and in mature osteoclasts. Carbenoxolone (CBX) functionally blocked gap junction communication as demonstrated by a scrape loading Lucifer Yellow dye transfer technique. CBX caused a dose-dependent inhibition (significant > or = 90 microM) of the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells formed in 7- to 8-day marrow cultures stimulated by parathyroid hormone (PTH; 10 nM) or forskolin (FSK; 1 microM). Furthermore, CBX (100 microM) significantly inhibited prostaglandin E2 (PGE2; 10 microM) and 1,25(OH)2-vitamin D3 stimulated osteoclast differentiation in the mouse bone marrow cultures. Consequently, quantitative real-time polymerase chain reaction (PCR) analysis demonstrated that CBX downregulated the expression of osteoclast phenotypic markers, but without having any significant effects on RANK, RANKL, and osteoprotegerin (OPG) mRNA expression. However, the results demonstrated that CBX significantly inhibits RANKL-stimulated (100 ng/ml) osteoclastogenesis in the mouse bone marrow cultures. Taken together, our results suggests that gap junctional diffusion of messenger molecules interacts with signaling pathways downstream RANKL in osteoclast differentiation. Further studies are required to define the precise mechanisms and molecular targets involved. Copyright 2006 Wiley-Liss, Inc.

National Category
Dentistry Dentistry
Identifiers
urn:nbn:se:umu:diva-8526 (URN)16639710 (PubMedID)
Available from: 2007-11-06 Created: 2007-11-06 Last updated: 2018-06-09Bibliographically approved
Smith, R., Ransjö, M., Tatarczuch, L., Song, S., Pagel, C., Morrison, J., . . . Mackie, E. (2004). Activation of protease-activated receptor-2 leads to inhibition of osteoclast differentiation.. Journal of Bone and Mineral Research, 19(3), 507-516
Open this publication in new window or tab >>Activation of protease-activated receptor-2 leads to inhibition of osteoclast differentiation.
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2004 (English)In: Journal of Bone and Mineral Research, ISSN 0884-0431, E-ISSN 1523-4681, Vol. 19, no 3, p. 507-516Article in journal (Refereed) Published
Abstract [en]

PAR-2 is expressed by osteoblasts and activated by proteases present during inflammation. PAR-2 activation inhibited osteoclast differentiation induced by hormones and cytokines in mouse bone marrow cultures and may protect bone from uncontrolled resorption. INTRODUCTION: Protease-activated receptor-2 (PAR-2), which is expressed by osteoblasts, is activated specifically by a small number of proteases, including mast cell tryptase and factor Xa. PAR-2 is also activated by a peptide (RAP) that corresponds to the "tethered ligand" created by cleavage of the receptor's extracellular domain. The effect of activating PAR-2 on osteoclast differentiation was investigated. MATERIALS AND METHODS: Mouse bone marrow cultures have been used to investigate the effect of PAR-2 activation on osteoclast differentiation induced by parathyroid hormone (PTH), 1,25 dihydroxyvitamin D3 [1,25(OH)2D3], and interleukin-11 (IL-11). Expression of PAR-2 by mouse bone marrow, mouse bone marrow stromal cell-enriched cultures, and the RAW264.7 osteoclastogenic cell line was demonstrated by RT-PCR. RESULTS: RAP was shown to inhibit osteoclast differentiation induced by PTH, 1,25(OH)2D3, or IL-11. Semiquantitative RT-PCR was used to investigate expression of mediators of osteoclast differentiation induced by PTH, 1,25(OH)2D3, or IL-11 in mouse bone marrow cultures and primary calvarial osteoblast cultures treated simultaneously with RAP. In bone marrow and osteoblast cultures treated with PTH, 1,25(OH)2D3, or IL-11, RAP inhibited expression of RANKL and significantly suppressed the ratio of RANKL:osteoprotegerin expression. Activation of PAR-2 led to reduced expression of prostaglandin G/H synthase-2 in bone marrow cultures treated with PTH, 1,25(OH)2D3, or IL-11. RAP inhibited PTH- or 1,25(OH)2D3-induced expression of IL-6 in bone marrow cultures. RAP had no effect on osteoclast differentiation in RANKL-treated RAW264.7 cells. CONCLUSION: These observations indicate that PAR-2 activation inhibits osteoclast differentiation by acting on cells of the osteoblast lineage to modulate multiple mediators of the effects of PTH, 1,25(OH)2D3, and IL-11. Therefore, the role of PAR-2 in bone may be to protect it from uncontrolled resorption by limiting levels of osteoclast differentiation.

Identifiers
urn:nbn:se:umu:diva-17337 (URN)10.1359/JBMR.0301248 (DOI)15040840 (PubMedID)
Available from: 2007-11-08 Created: 2007-11-08 Last updated: 2018-06-09Bibliographically approved
Brage, M., Lie, A., Ransjö, M., Kasprzykowski, F., Kasprzykowska, R., Abrahamson, M., . . . Lerner, U. (2004). Osteoclastogenesis is decreased by cysteine proteinase inhibitors. Bone, 34(3), 412-24
Open this publication in new window or tab >>Osteoclastogenesis is decreased by cysteine proteinase inhibitors
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2004 (English)In: Bone, ISSN 8756-3282, E-ISSN 1873-2763, Vol. 34, no 3, p. 412-24Article in journal (Refereed) Published
Abstract [en]

The effects of cystatin C and other cysteine proteinase inhibitors on osteoclast formation and differentiation have been investigated. Cystatin C decreased osteoclast formation stimulated by parathyroid hormone (PTH), 1,25(OH)2-vitamin D3 or interleukin-6 (IL-6) (in the presence of its soluble receptor) as assessed by the number of tartrate-resistant acid phosphatase (TRAP+) multinucleated cells in mouse bone marrow cultures. The inhibitory effect was associated with decreased mRNA expression for the calcitonin receptor as well as decreased number of specific binding sites for 125I-calcitonin, and without any effect on the mRNA expression of receptor activator of nuclear factor kappaB (NF-kappaB) ligand (RANKL). Similarly, the cysteine proteinase inhibitors leupeptin, E-64 and benzyloxycarbonyl-Phe-Ala-diazomethane (Z-FA-CHN2) decreased PTH-stimulated formation of TRAP+ multinucleated cells and binding of 125I-calcitonin. A peptidyl derivative synthesized to mimic part of the proteinase-binding site of cystatin C (benzyloxycarbonyl-Arg-Leu-Val-Gly-diazomethane, or Z-RLVG-CHN2) also decreased PTH-stimulated osteoclast formation. In a 9-day culture, addition of cystatin C during the last 5 days was sufficient to cause substantial inhibition of osteoclast formation. Cystatin C-induced decrease of osteoclast formation was associated with enhanced number of F4/80-positive macrophages and increased mRNA expression of the macrophage receptor c-fms in the bone marrow culture. Osteoclast formation in mouse bone marrow cultures as well as in mouse spleen cell cultures, stimulated by macrophage colony-stimulating factor (M-CSF) and RANKL was also decreased by different cysteine proteinase inhibitors. In addition, cystatin C inhibited M-CSF/RANKL induction of calcitonin receptor mRNA in spleen cell cultures. The inhibitory effect by cystatin C in spleen cells was associated with decreased mRNA expression of RANK and the transcription factor NFAT2. It is concluded that cysteine proteinase inhibitors decrease formation of osteoclasts by interfering at a late stage of pre-osteoclast differentiation.

Identifiers
urn:nbn:se:umu:diva-17339 (URN)10.1016/j.bone.2003.11.018 (DOI)15003789 (PubMedID)
Available from: 2007-11-27 Created: 2007-11-27 Last updated: 2018-06-09Bibliographically approved
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