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Skogström, Oskar
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Publications (6 of 6) Show all publications
Zulfugarov, I. S., Tovuu, A., Kim, C.-Y., Vo, K. T., Ko, S. Y., Hall, M., . . . Lee, C.-H. (2016). Enhanced resistance of PsbS-deficient rice (Oryza sativa L.) to fungal and bacterial pathogens. Journal of Plant Biology, 59(6), 616-626
Open this publication in new window or tab >>Enhanced resistance of PsbS-deficient rice (Oryza sativa L.) to fungal and bacterial pathogens
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2016 (English)In: Journal of Plant Biology, ISSN 1226-9239, Vol. 59, no 6, p. 616-626Article in journal (Refereed) Published
Abstract [en]

The 22-kDa PsbS protein of Photosystem II is involved in nonphotochemical quenching (NPQ) of chlorophyll fluorescence. Genome-wide analysis of the expression pattern in PsbS knockout (KO) rice plants showed that a lack of this protein led to changes in the transcript levels of 406 genes, presumably a result of superoxide produced in the chloroplasts. The top Gene Ontology categories, in which expression was the most differential, included 'Immune response', 'Response to jasmonic acid', and 'MAPK cascade'. From those genes, we randomly selected nine that were up-regulated. Our microarray results were confirmed by quantitative RT-PCR analysis. The KO and PsbS RNAi (knockdown) plants were more resistant to pathogens Magnaporthe oryzae PO6-6 and Xanthomonas oryzae pv. oryzae than either the wild-type plants or PsbS-overexpressing transgenic line. These findings suggest that superoxide production might be the reason that these plants have greater pathogen resistance to fungal and bacterial pathogens in the absence of energy-dependent NPQ. For example, a high level of cell wall lignification in the KO mutants was possibly due to enhanced superoxide production. Our data indicate that certain abiotic stress-induced reactive oxygen species can promote specific signaling pathways, which then activate a defense mechanism against biotic stress in PsbS-KO rice plants.

Place, publisher, year, edition, pages
Springer Berlin/Heidelberg, 2016
Keywords
Biotic stress tolerance, Microarray, Nonphotochemical quenching, PsbS, Rice, Superoxide
National Category
Botany Genetics
Identifiers
urn:nbn:se:umu:diva-129724 (URN)10.1007/s12374-016-0068-6 (DOI)000389986200006 ()
Available from: 2017-01-10 Created: 2017-01-09 Last updated: 2018-06-09Bibliographically approved
Frenkel, M., Külheim, C., Johansson Jänkänpää, H., Skogström, O., Dall Osto, L., Ågren, J., . . . Jansson, S. (2009). Improper excess light energy dissipation in Arabidopsis results in a metabolic reprogramming. BMC Plant Biology, 9(12), 1-16
Open this publication in new window or tab >>Improper excess light energy dissipation in Arabidopsis results in a metabolic reprogramming
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2009 (English)In: BMC Plant Biology, ISSN 1471-2229, E-ISSN 1471-2229, Vol. 9, no 12, p. 1-16Article in journal (Refereed) Published
Abstract [en]

Background: Plant performance is affected by the level of expression of PsbS, a key photoprotective protein involved in the process of feedback de-excitation (FDE), or the qE component of non-photochemical quenching, NPQ.

Results: In studies presented here, under constant laboratory conditions the metabolite profiles of leaves of wild-type Arabidopsis thaliana and plants lacking or overexpressing PsbS were very similar, but under natural conditions their differences in levels of PsbS expression were associated with major changes in metabolite profiles. Some carbohydrates and amino acids differed ten-fold in abundance between PsbS-lacking mutants and over-expressers, with wild-type plants having intermediate amounts, showing that a metabolic shift had occurred. The transcriptomes of the genotypes also varied under field conditions, and the genes induced in plants lacking PsbS were similar to those reportedly induced in plants exposed to ozone stress or treated with methyl jasmonate (MeJA). Genes involved in the biosynthesis of JA were up-regulated, and enzymes involved in this pathway accumulated. JA levels in the undamaged leaves of field-grown plants did not differ between wild-type and PsbS-lacking mutants, but they were higher in the mutants when they were exposed to herbivory.

Conclusion: These findings suggest that lack of FDE results in increased photooxidative stress in the chloroplasts of Arabidopsis plants grown in the field, which elicits a response at the transcriptome level, causing a redirection of metabolism from growth towards defence that resembles a MeJA/JA response.

Keywords
Arabidopsis, PsbS, photosynthesis, abiotic and biotic stress
National Category
Biological Sciences
Research subject
Physiological Botany
Identifiers
urn:nbn:se:umu:diva-48563 (URN)10.1186/1471-2229-9-12 (DOI)000264250500001 ()19171025 (PubMedID)
Funder
FormasSwedish Research Council
Available from: 2011-10-24 Created: 2011-10-24 Last updated: 2018-06-08Bibliographically approved
Street, N. R., Skogström, O., Sjödin, A., Tucker, J., Rodri­guez-Acosta, M., Nilsson, P., . . . Taylor, G. (2006). The genetics and genomics of the drought response in Populus.. The Plant Journal, 48(3), 321-41
Open this publication in new window or tab >>The genetics and genomics of the drought response in Populus.
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2006 (English)In: The Plant Journal, ISSN 0960-7412, Vol. 48, no 3, p. 321-41Article in journal (Refereed) Published
Abstract [en]

The genetic nature of tree adaptation to drought stress was examined by utilizing variation in the drought response of a full-sib second generation (F2) mapping population from a cross between Populus trichocarpa (93-968) and P. deltoides Bart (ILL-129) and known to be highly divergent for a vast range of phenotypic traits. We combined phenotyping, quantitative trait loci (QTL) analysis and microarray experiments to demonstrate that ‘genetical genomics’ can be used to provide information on adaptation at the species level. The grandparents and F2 population were subjected to soil drying, and contrasting responses to drought across genotypes, including leaf coloration, expansion and abscission, were observed, and QTL for these traits mapped. A subset of extreme genotypes exhibiting extreme sensitivity and insensitivity to drought on the basis of leaf abscission were defined, and microarray experiments conducted on these genotypes and the grandparent species. The extreme genotype groups induced a different set of genes: 215 and 125 genes differed in their expression response between groups in control and drought, respectively, suggesting species adaptation at the gene expression level. Co-location of differentially expressed genes with drought-specific and drought-responsive QTLs was examined, and these may represent candidate genes contributing to the variation in drought response.

Keywords
Expressed Sequence Tags, Genome; Plant, Natural Disasters, Populus/*genetics, Quantitative Trait Loci, RNA; Messenger/genetics, Species Specificity
Identifiers
urn:nbn:se:umu:diva-14205 (URN)doi:10.1111/j.1365-313X.2006.02864.x (DOI)17005011 (PubMedID)
Available from: 2007-08-10 Created: 2007-08-10 Last updated: 2018-06-09Bibliographically approved
Sjödin, A., Bylesjö, M., Skogström, O., Eriksson, D., Nilsson, P., Rydén, P., . . . Karlsson, J. (2006). UPSC-BASE: Populus transcriptomics online. The Plant Journal, 48(5), 806-817
Open this publication in new window or tab >>UPSC-BASE: Populus transcriptomics online
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2006 (English)In: The Plant Journal, ISSN 0960-7412, E-ISSN 1365-313X, Vol. 48, no 5, p. 806-817Article in journal (Refereed) Published
Abstract [en]

The increasing accessibility and use of microarrays in transcriptomics has accentuated the need for purpose-designed storage and analysis tools. Here we present UPSC-BASE, a database for analysis and storage of Populus DNA microarray data. A microarray analysis pipeline has also been established to allow consistent and efficient analysis (from small to large scale) of samples in various experimental designs. A range of optimized experimental protocols is provided for each step in generating the data. Within UPSC-BASE, researchers can perform standard and advanced microarray analysis procedures in a user-friendly environment. Background corrections, normalizations, quality-control tools, visualizations, hypothesis tests and export tools are provided without requirements for expert-level knowledge. Although the database has been developed primarily for handling Populus DNA microarrays, most of the tools are generic and can be used for other types of microarray. UPSC-BASE is also a repository of Populus microarray information, providing data from 21 experiments on a total of 407 microarray hybridizations in the public domain of the database. There are also an additional 10 experiments containing 347 hybridizations, where the automatically analysed data are searchable.

 

Place, publisher, year, edition, pages
Oxford: Blackwell, 2006
Keywords
Databases, Genetic, Gene Expression Profiling, Gene Expression Regulation, Plant, Internet, Populus/*genetics
Identifiers
urn:nbn:se:umu:diva-2741 (URN)10.1111/j.1365-313X.2006.02920.x (DOI)
Available from: 2007-11-07 Created: 2007-11-07 Last updated: 2018-06-09Bibliographically approved
Taylor, G., Street, N. R., Tricker, P. J., Sjödin, A., Graham, L., Skogström, O., . . . Jansson, S. (2005). The transcriptome of Populus in elevated CO2.. New Phytologist, 167(1), 143-54
Open this publication in new window or tab >>The transcriptome of Populus in elevated CO2.
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2005 (English)In: New Phytologist, ISSN 0028-646X, Vol. 167, no 1, p. 143-54Article in journal (Refereed) Published
Abstract [en]

The consequences of increasing atmospheric carbon dioxide for long-term adaptation of forest ecosystems remain uncertain, with virtually no studies undertaken at the genetic level. A global analysis using cDNA microarrays was conducted following 6 yr exposure of Populus × euramericana (clone I-214) to elevated [CO2] in a FACE (free-air CO2 enrichment) experiment.

Gene expression was sensitive to elevated [CO2] but the response depended on the developmental age of the leaves, and < 50 transcripts differed significantly between different CO2 environments. For young leaves most differentially expressed genes were upregulated in elevated [CO2], while in semimature leaves most were downregulated in elevated [CO2].

For transcripts related only to the small subunit of Rubisco, upregulation in LPI 3 and downregulation in LPI 6 leaves in elevated CO2 was confirmed by anova. Similar patterns of gene expression for young leaves were also confirmed independently across year 3 and year 6 microarray data, and using real-time RT–PCR.

This study provides the first clues to the long-term genetic expression changes that may occur during long-term plant response to elevated CO2.

Keywords
Adaptation; Physiological, Atmosphere, Carbon Dioxide/*pharmacology, Expressed Sequence Tags, Gene Expression Profiling, Gene Expression Regulation; Plant/*drug effects, Oligonucleotide Array Sequence Analysis, Populus/*drug effects/*genetics/metabolism, Time Factors
Identifiers
urn:nbn:se:umu:diva-14222 (URN)doi:10.1111/j.1469-8137.2005.01450.x (DOI)15948837 (PubMedID)
Available from: 2007-05-24 Created: 2007-05-24 Last updated: 2018-06-09Bibliographically approved
Frenkel, M., Külheim, C., Johansson Jänkänpää, H., Skogström, O., Frigerio, S., Ågren, J., . . . Jansson, S.Improper regulation of light harvesting in Arabidopsis results in a metabolic reprogramming.
Open this publication in new window or tab >>Improper regulation of light harvesting in Arabidopsis results in a metabolic reprogramming
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(English)Manuscript (preprint) (Other (popular science, discussion, etc.))
Identifiers
urn:nbn:se:umu:diva-3206 (URN)
Available from: 2008-05-13 Created: 2008-05-13 Last updated: 2018-06-09Bibliographically approved
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