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Davoine, Céline
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Publications (3 of 3) Show all publications
Davoine, C., Abreu, I. N., Khajeh, K., Blomberg, J., Kidd, B. N., Kazan, K., . . . Björklund, S. (2017). Functional metabolomics as a tool to analyze Mediator function and structure in plants. PLoS ONE, 12(6), Article ID e0179640.
Open this publication in new window or tab >>Functional metabolomics as a tool to analyze Mediator function and structure in plants
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2017 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 6, article id e0179640Article in journal (Refereed) Published
Abstract [en]

Mediator is a multiprotein transcriptional co-regulator complex composed of four modules; Head, Middle, Tail, and Kinase. It conveys signals from promoter-bound transcriptional regulators to RNA polymerase II and thus plays an essential role in eukaryotic gene regulation. We describe subunit localization and activities of Mediator in Arabidopsis through metabolome and transcriptome analyses from a set of Mediator mutants. Functional metabolomic analysis based on the metabolite profiles of Mediator mutants using multivariate statistical analysis and heat-map visualization shows that different subunit mutants display distinct metabolite profiles, which cluster according to the reported localization of the corresponding subunits in yeast. Based on these results, we suggest localization of previously unassigned plant Mediator subunits to specific modules. We also describe novel roles for individual subunits in development, and demonstrate changes in gene expression patterns and specific metabolite levels in med18 and med25, which can explain their phenotypes. We find that med18 displays levels of phytoalexins normally found in wild type plants only after exposure to pathogens. Our results indicate that different Mediator subunits are involved in specific signaling pathways that control developmental processes and tolerance to pathogen infections.

Place, publisher, year, edition, pages
Public Library Science, 2017
National Category
Developmental Biology Genetics
Identifiers
urn:nbn:se:umu:diva-140237 (URN)10.1371/journal.pone.0179640 (DOI)000404135800047 ()28640868 (PubMedID)
Available from: 2017-10-19 Created: 2017-10-19 Last updated: 2018-06-09Bibliographically approved
Fallath, T., Kidd, B. N., Stiller, J., Davoine, C., Björklund, S., Manners, J. M., . . . Schenk, P. M. (2017). MEDIATOR18 and MEDIATOR20 confer susceptibility to Fusarium oxysporum in Arabidopsis thaliana. PLoS ONE, 12(4), Article ID e0176022.
Open this publication in new window or tab >>MEDIATOR18 and MEDIATOR20 confer susceptibility to Fusarium oxysporum in Arabidopsis thaliana
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2017 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 4, article id e0176022Article in journal (Refereed) Published
Abstract [en]

The conserved protein complex known as Mediator conveys transcriptional signals by acting as an intermediary between transcription factors and RNA polymerase II. As a result, Mediator subunits play multiple roles in regulating developmental as well as abiotic and biotic stress pathways. In this report we identify the head domain subunits MEDIATOR18 and MEDIATOR20 as important susceptibility factors for Fusarium oxysporum infection in Arabidopsis thaliana. Mutants of MED18 and MED20 display down-regulation of genes associated with jasmonate signaling and biosynthesis while up-regulation of salicylic acid associated pathogenesis related genes and reactive oxygen producing and scavenging genes. We propose that MED18 and MED20 form a sub-domain within Mediator that controls the balance of salicylic acid and jasmonate associated defense pathways.

Place, publisher, year, edition, pages
PUBLIC LIBRARY SCIENCE, 2017
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:umu:diva-136206 (URN)10.1371/journal.pone.0176022 (DOI)000400308800020 ()28441405 (PubMedID)
Available from: 2017-07-03 Created: 2017-07-03 Last updated: 2018-06-09Bibliographically approved
Elfving, N., Davoine, C., Benlloch, R., Blomberg, J., Brännström, K., Müller, D., . . . Björklund, S. (2011). The Arabidopsis thaliana Med25 mediator subunit integrates environmental cues to control plant development. Proceedings of the National Academy of Sciences of the United States of America, 108(20), 8245-8250
Open this publication in new window or tab >>The Arabidopsis thaliana Med25 mediator subunit integrates environmental cues to control plant development
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2011 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 108, no 20, p. 8245-8250Article in journal (Refereed) Published
Abstract [en]

Development in plants is controlled by abiotic environmental cues such as day length, light quality, temperature, drought, and salinity. These signals are sensed by a variety of systems and transmitted by different signal transduction pathways. Ultimately, these pathways are integrated to control expression of specific target genes, which encode proteins that regulate development and differentiation. The molecular mechanisms for such integration have remained elusive. We here show that a linear 130-amino-acids-long sequence in the Med25 subunit of the Arabidopsis thaliana Mediator is a common target for the drought response element binding protein 2A, zinc finger homeodomain 1, and Myb-like transcription factors which are involved in different stress response pathways. In addition, our results show that Med25 together with drought response element binding protein 2A also function in repression of PhyB-mediated light signaling and thus integrate signals from different regulatory pathways.

Keywords
transcriptional regulation, phytochrome flowering time 1, RNA polymerase II
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-46758 (URN)10.1073/pnas.1002981108 (DOI)21536906 (PubMedID)
Available from: 2011-09-13 Created: 2011-09-13 Last updated: 2018-06-08Bibliographically approved
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