umu.sePublications
Change search
Link to record
Permanent link

Direct link
BETA
Zeng, Yingxu
Publications (2 of 2) Show all publications
Zeng, Y., Mtintsilana, A., Goedecke, J. H., Micklesfield, L. K., Olsson, T. & Chorell, E. (2019). Alterations in the metabolism of phospholipids, bile acids and branched-chain amino acids predicts development of type 2 diabetes in black South African women: a prospective cohort study. Metabolism: Clinical and Experimental, 95, 57-64
Open this publication in new window or tab >>Alterations in the metabolism of phospholipids, bile acids and branched-chain amino acids predicts development of type 2 diabetes in black South African women: a prospective cohort study
Show others...
2019 (English)In: Metabolism: Clinical and Experimental, ISSN 0026-0495, E-ISSN 1532-8600, Vol. 95, p. 57-64Article in journal (Refereed) Published
Abstract [en]

Background: South Africa (SA) has the highest global projected increase in diabetes risk. Factors typically associated with insulin resistance and type 2 diabetes risk in Caucasians are not significant correlates in black African populations. Therefore, we aimed to identify circulating metabolite patterns that predict type 2 diabetes development in this high-risk, yet understudied SA population.

Methods: We conducted a prospective cohort study in black SA women with normal glucose tolerance (NGT). Participants were followed for 13 years and developed (i) type 2 diabetes (n = 20, NGT-T2D), (ii) impaired glucose tolerance (IGT) (n = 27, NGT-IGT), or (iii) remained NGT (n = 28, NGT-NGT). Mass-spectrometry based metabolomics and multivariate analyses were used to elucidate metabolite patterns at baseline and at follow-up that were associated with type 2 diabetes development.

Results: Metabolites of phospholipid, bile acid and branched-chain amino acid (BCAA) metabolism, differed significantly between the NGT-T2D and NGT-NGT groups. At baseline: the NGT-T2D group had i) a higher lysophosphatidylcholine:lysophosphatidylethanolamine ratio containing linoleic acid (LPC(C18:2):LPE(C18:2)), ii) lower proliferation-related bile acids (ursodeoxycholic- and chenodeoxycholic acid), iii) higher levels of leucine and its catabolic intermediates (ketoleucine and C5-carnitine), compared to the NGT-NGT group. At follow-up: the NGT-T2D group had i) lower LPC(C18:2) levels, ii) higher apoptosis-related bile acids (deoxycholic- and glycodeoxycholic acid), and iii) higher levels of all BCAAs and their catabolic intermediates.

Conclusions: Changes in lysophospholipid metabolism and the bile acid pool occur during the development of type 2 diabetes in black South African women. Further, impaired leucine catabolism precedes valine and isoleucine catabolism in the development of type 2 diabetes. These metabolite patterns can be useful to identify and monitor type 2 diabetes risk >10 years prior to disease onset and provide insight into the pathophysiology of type 2 diabetes in this high risk, but under-studied population.

Place, publisher, year, edition, pages
Elsevier, 2019
Keywords
Type 2 diabetes, Insulin resistance, Metabolomics, Branched-chain amino acids, Phospholipids, Bile acids
National Category
Endocrinology and Diabetes
Identifiers
urn:nbn:se:umu:diva-160603 (URN)10.1016/j.metabol.2019.04.001 (DOI)000470307800007 ()30954560 (PubMedID)
Funder
Swedish Research CouncilWellcome trust
Available from: 2019-06-24 Created: 2019-06-24 Last updated: 2019-06-24Bibliographically approved
Araujo, P., Tilahun, E. & Zeng, Y. (2018). A novel strategy for discriminating marine oils by using the positional distribution (sn-1, sn-2, sn-3) of omega-3 polyunsaturated fatty acids in triacylglycerols. Talanta: The International Journal of Pure and Applied Analytical Chemistry, 182, 32-37
Open this publication in new window or tab >>A novel strategy for discriminating marine oils by using the positional distribution (sn-1, sn-2, sn-3) of omega-3 polyunsaturated fatty acids in triacylglycerols
2018 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 182, p. 32-37Article in journal (Refereed) Published
Abstract [en]

A novel strategy for discriminating genuine and adulterated marine oils is proposed. The strategy consists of i) determining the stereospecific distribution (sn-1, sn-2 and sn-3) of omega 3 polyunsaturated fatty acids (omega-3 PUFA) on the backbone of triacylglycerols by using liquid chromatography tandem mass spectrometry; ii) transforming the qualitative stereospecific information into quantitative data by means of a novel strategy; iii) analyzing the transformed data by principal component analysis. The proposed strategy was tested on pure oils (seal, salmon, cod liver, sandeel, blue whiting, herring), a mixture of blue whiting, herring, sandeel and Norway pout and some intentionally adulterated oils. In addition, some published krill oil data were analyzed to confirm the reliability of the new approach.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Triacylglycerol, Positional distribution, Fish oil, Marine oil, Liquid chromatography mass spectrometry, ulteration
National Category
Ecology
Identifiers
urn:nbn:se:umu:diva-146549 (URN)10.1016/j.talanta.2018.01.030 (DOI)000428229200003 ()29501159 (PubMedID)
Available from: 2018-05-15 Created: 2018-05-15 Last updated: 2019-06-05Bibliographically approved
Organisations

Search in DiVA

Show all publications