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Law, S. R., Chrobok, D., Juvany, M., Delhomme, N., Lindén, P., Brouwer, B., . . . Keech, O. (2018). Darkened leaves use different metabolic strategies for senescence and survival. Plant Physiology, 177(1), 132-150
Open this publication in new window or tab >>Darkened leaves use different metabolic strategies for senescence and survival
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2018 (English)In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 177, no 1, p. 132-150Article in journal (Refereed) Published
Abstract [en]

In plants, an individually darkened leaf initiates senescence much more rapidly than a leaf from a whole darkened plant. Combining transcriptomic and metabolomic approaches in Arabidopsis (Arabidopsis thaliana), we present an overview of the metabolic strategies that are employed in response to different darkening treatments. Under darkened plant conditions, the perception of carbon starvation drove a profound metabolic readjustment in which branched-chain amino acids and potentially monosaccharides released from cell wall loosening became important substrates for maintaining minimal ATP production. Concomitantly, the increased accumulation of amino acids with a high nitrogen-carbon ratio may provide a safety mechanism for the storage of metabolically derived cytotoxic ammonium and a pool of nitrogen for use upon returning to typical growth conditions. Conversely, in individually darkened leaf, the metabolic profiling that followed our 13C-enrichment assays revealed a temporal and differential exchange of metabolites, including sugars and amino acids, between the darkened leaf and the rest of the plant. This active transport could be the basis for a progressive metabolic shift in the substrates fueling mitochondrial activities, which are central to the catabolic reactions facilitating the retrieval of nutrients from the senescing leaf. We propose a model illustrating the specific metabolic strategies employed by leaves in response to these two darkening treatments, which support either rapid senescence or a strong capacity for survival.

Keywords
Arabidopsis thaliana, senescence, metabolism, dark induced senescence, survival
National Category
Botany
Research subject
biology
Identifiers
urn:nbn:se:umu:diva-147675 (URN)10.1104/pp.18.00062 (DOI)000431347500015 ()29523713 (PubMedID)
Available from: 2018-05-14 Created: 2018-05-14 Last updated: 2018-06-09Bibliographically approved
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Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-1093-3317

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