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Valeriano, Valerie Diane V.ORCID iD iconorcid.org/0000-0002-4396-346x
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Publications (6 of 6) Show all publications
Valeriano, V. D., Oh, J. K., Bagon, B., Kim, H. & Kang, D.-K. (2019). Comparative genomic analysis of Lactobacillus mucosae LM1 identifies potential niche-specific genes and pathways for gastrointestinal adaptation. Genomics, 111(1), 24-33
Open this publication in new window or tab >>Comparative genomic analysis of Lactobacillus mucosae LM1 identifies potential niche-specific genes and pathways for gastrointestinal adaptation
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2019 (English)In: Genomics, ISSN 0888-7543, E-ISSN 1089-8646, Vol. 111, no 1, p. 24-33Article in journal (Refereed) Published
Abstract [en]

Lactobacillus mucosae is currently of interest as putative probiotics due to their metabolic capabilities and ability to colonize host mucosal niches. L. mucosae LM1 has been studied in its functions in cell adhesion and pathogen inhibition, etc. It demonstrated unique abilities to use energy from carbohydrate and non-carbohydrate sources. Due to these functions, we report the first complete genome sequence of an L. mucosae strain, Lmucosae LM1. Analysis of the pan-genome in comparison with closely-related Lactobacillus species identified a complete glycogen metabolism pathway, as well as folate biosynthesis, complementing previous proteomic data on the LM1 strain. It also revealed common and unique niche-adaptation genes among the various L. mucosae strains. The aim of this study was to derive genomic information that would reveal the probable mechanisms underlying the probiotic effect of L. mucosae LM1, and provide a better understanding of the nature of L. mucosae sp.

Place, publisher, year, edition, pages
Elsevier, 2019
Keywords
Lactobacillus mucosae, Comparative genomics, Probiotics
National Category
Microbiology
Identifiers
urn:nbn:se:umu:diva-145020 (URN)10.1016/j.ygeno.2017.12.009 (DOI)000455225100004 ()
Available from: 2018-02-16 Created: 2018-02-16 Last updated: 2019-01-28Bibliographically approved
Bagon, B. B., Valeriano, V. D., Oh, J. K., Pajarillo, E. A., Cho, C.-S. & Kang, D.-K. (2018). Comparative exoproteome analyses of Lactobacillus spp. reveals species-and strain-specific proteins involved in their extracellular interaction and probiotic potential. Lebensmittel-Wissenschaft + Technologie, 93, 420-426
Open this publication in new window or tab >>Comparative exoproteome analyses of Lactobacillus spp. reveals species-and strain-specific proteins involved in their extracellular interaction and probiotic potential
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2018 (English)In: Lebensmittel-Wissenschaft + Technologie, ISSN 0023-6438, E-ISSN 1096-1127, Vol. 93, p. 420-426Article in journal (Refereed) Published
Abstract [en]

Due to their health-promoting effects, the probiotic applications of lactobacilli have been investigated. Aside from their basic cellular functions, the extracellular activities of probiotic lactobacilli influence their establishment and interaction with the host. Many extracellular proteins play important roles in bacterial colonization and survival in their host's gastrointestinal environment. In this study, we compared the exoproteome of three lactobacillus strains isolated from the gastrointestinal tracts of pigs and chickens, namely, Lactobacillus mucosae LM1, Ljohnsonii PF01, and Ljohnsonii C1–10. Extracellular proteins collected in the mid-logarithmic growth phase were identified and quantified using a Q Exactive™ Orbitrap mass spectrometer (MS). Of 99 total extracellular proteins, 83% belonged to Lmucosae LM1; Ljohnsonii PF01 strains had fewer extracellular proteins. Enolase, which is involved in the glycolysis pathway and has moonlighting functions in the adhesion of probiotic bacteria, was found in the core exoproteomes of the three strains. The most abundant proteins of each strain were phosphoketolase in Lmucosae LM1, aggregation-promoting factor in Ljohnsonii PF01, and levansucrase in Ljohnsonii C1–10. The observed differences between these three exoproteomes in terms of cellular and molecular function elucidate the extracellular activities of these isolates.

Keywords
Extracellular proteome, Lactobacillus johnsonii, Lactobacillus mucosae, Lactobacilli, Probiotics
National Category
Agricultural and Veterinary sciences
Identifiers
urn:nbn:se:umu:diva-146378 (URN)10.1016/j.lwt.2018.03.069 (DOI)000432640600058 ()
Available from: 2018-04-06 Created: 2018-04-06 Last updated: 2018-12-11Bibliographically approved
Valeriano, V. D., Parungao-Balolong, M. & Kang, D.-K. (2017). Probiotic Roles of Lactobacillus spp. in Swine: Insights from Gut Microbiota. Journal of Applied Microbiology, 122(3), 554-567
Open this publication in new window or tab >>Probiotic Roles of Lactobacillus spp. in Swine: Insights from Gut Microbiota
2017 (English)In: Journal of Applied Microbiology, ISSN 1364-5072, E-ISSN 1365-2672, Vol. 122, no 3, p. 554-567Article, review/survey (Other academic) Published
Abstract [en]

The use of lactobacilli as probiotics in swine has been gaining attention due to their ability to improve growth performance and carcass quality, prevent gastrointestinal infection and most importantly, their 'generally recognized as safe' status. Previous studies support the potential of lactobacilli to regulate host immune systems, enhance gut metabolic capacities and maintain balance in the gut microbiota. Research on swine gut microbiota has revealed complex gut microbial community structure and showed the importance of Lactobacillus to the host's health. However, the species- and strain-specific characteristics of lactobacilli that confer probiotic benefits are still not well understood. The diversity of probiotic traits in a complex gut ecosystem makes it challenging to infer the relationships between specific functions of Lactobacillus sp. and host health. In this review, we provide an overview of how lactobacilli play a pivotal role in the swine gut ecosystem and identify key characteristics that influence gut microbial community structure and the health of pigs. In addition, based on recent and ongoing meta-omics and omics research on the gut microbiota of pigs, we suggest a workflow combining culture-dependent and culture-independent approaches for more effective selection of probiotic lactobacilli.

Keywords
Lactobacillus ; intestinal microbiology; lactic acid bacteria; probiotics; veterinary
National Category
Agricultural and Veterinary sciences
Research subject
Microbiology
Identifiers
urn:nbn:se:umu:diva-145897 (URN)10.1111/jam.13364 (DOI)
Available from: 2018-03-20 Created: 2018-03-20 Last updated: 2018-06-09
Pajarillo, E. A., Kim, S. H., Valeriano, V. D., Lee, J. Y. & Kang, D.-K. (2017). Proteomic View of the Crosstalk between Lactobacillus mucosae and Intestinal Epithelial Cells in Co-culture Revealed by Q Exactive-Based Quantitative Proteomics. Frontiers in Microbiology, 8, Article ID 2459.
Open this publication in new window or tab >>Proteomic View of the Crosstalk between Lactobacillus mucosae and Intestinal Epithelial Cells in Co-culture Revealed by Q Exactive-Based Quantitative Proteomics
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2017 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 8, article id 2459Article in journal (Refereed) Published
Abstract [en]

Lactobacilli are bacteria that are beneficial to host health, but information on communication between Lactobacilli and host cells in the intestine is lacking. In this study, we examined the proteomes of the Lactobacillus mucosae strain LM1, as a model of beneficial bacteria, and the intestinal porcine epithelial cell line (IPEC-J2) after co-culture. Label-free proteomics demonstrated the high-throughput capability of the technique, and robust characterization of the functional profiles and changes in the bacteria and intestinal cells was achieved in pure and mixed cultures. After co-culture, we identified totals of 376 and 653 differentially expressed proteins in the LM1 and IPEC-J2 proteomes, respectively. The major proteomic changes in the LM1 strain occurred in the functional categories of transcription, general function, and translation, whereas those in IPEC-J2 cells involved metabolic and cellular processes, and cellular component organization/biogenesis. Among them, elongation factor Tu, glyceraldehyde 3-phosphate dehydrogenase, and phosphocarrier protein HPr, which are known to be involved in bacterial adhesion, were upregulated in LM1. In contrast, proteins involved in tight junction assembly, actin organization, and genetic information processing (i.e., histones and signaling pathways) were significantly upregulated in IPEC-J2 cells. Furthermore, we identified functional pathways that are possibly involved in host–microbe crosstalk and response. These findings will provide novel insights into host–bacteria communication and the molecular mechanism of probiotic establishment in the intestine.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2017
Keywords
Lactobacillus mucosae, adhesion, host–microbe interaction, porcine intestinal epithelial cells, label-free proteomics
National Category
Microbiology
Research subject
Microbiology
Identifiers
urn:nbn:se:umu:diva-145021 (URN)10.3389/fmicb.2017.02459 (DOI)
Available from: 2018-02-16 Created: 2018-02-16 Last updated: 2018-06-09Bibliographically approved
Pajarillo, E. A., Valeriano, V. D., Kim, S. H., Lee, J.-Y. & Kang, D.-K. (2015). Quantitative Proteogenomics and the Reconstruction of the Metabolic Pathway in Lactobacillus mucosae LM1. Hangug chugsan sigpum haghoeji = Korean journal for food science of animal resources, 35(5), 692-702
Open this publication in new window or tab >>Quantitative Proteogenomics and the Reconstruction of the Metabolic Pathway in Lactobacillus mucosae LM1
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2015 (English)In: Hangug chugsan sigpum haghoeji = Korean journal for food science of animal resources, ISSN 1225-8563, Vol. 35, no 5, p. 692-702Article in journal (Refereed) Published
Abstract [en]

Lactobacillus mucosae is a natural resident of the gastrointestinal tract of humans and animals and a potential probiotic bacterium. To understand the global protein expression profile and metabolic features of L. mucosae LM1 in the early stationary phase, the QExactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer was used. Characterization of the intracellular proteome identified 842 proteins, accounting for approximately 35% of the 2,404 protein-coding sequences in the complete genome of L. mucosae LM1. Proteome quantification using QExactive™ Orbitrap MS detected 19 highly abundant proteins (> 1.0% of the intracellular proteome), including CysK (cysteine synthase, 5.41%) and EF-Tu (elongation factor Tu, 4.91%), which are involved in cell survival against environmental stresses. Metabolic pathway annotation of LM1 proteome using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database showed that half of the proteins expressed are important for basic metabolic and biosynthetic processes, and the other half might be structurally important or involved in basic cellular processes. In addition, glycogen biosynthesis was activated in the early stationary phase, which is important for energy storage and maintenance. The proteogenomic data presented in this study provide a suitable reference to understand the protein expression pattern of lactobacilli in standard conditions.

Keywords
Lactobacillus mucosae, QExactiveTM Orbitrap, mass spectrometry, proteome, metabolic pathway
National Category
Agricultural and Veterinary sciences
Research subject
Microbiology
Identifiers
urn:nbn:se:umu:diva-145896 (URN)10.5851/kosfa.2015.35.5.692 (DOI)
Available from: 2018-03-20 Created: 2018-03-20 Last updated: 2018-06-09
Lee, J. H., Valeriano, V. D., Shin, Y.-r., Chae, J. P., Kim, G.-B., Ham, J.-S., . . . Kang, D.-K. (2012). Genome Sequence of Lactobacillus mucosae LM1, Isolated from Piglet Feces. Journal of Bacteriology, 194(§7), 4766
Open this publication in new window or tab >>Genome Sequence of Lactobacillus mucosae LM1, Isolated from Piglet Feces
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2012 (English)In: Journal of Bacteriology, ISSN 0021-9193, E-ISSN 1098-5530, Vol. 194, no §7, p. 4766-Article in journal (Other academic) Published
Abstract [en]

Lactobacillus mucosae LM1,isolated from stool samples of a healthy piglet, displays good in vitro mucin adhesion and antimicrobial activity against pathogenic bacteria.To elucidate its antimicrobial effects and to find its epithelial cell and mucin adhesion genes, the genomic sequence of L. mucosae LM1 was investigated.

Keywords
Lactobacillus, Genome Sequence, Probiotics
National Category
Agricultural and Veterinary sciences
Research subject
Microbiology
Identifiers
urn:nbn:se:umu:diva-145895 (URN)10.1128/JB.01011-12 (DOI)
Available from: 2018-03-20 Created: 2018-03-20 Last updated: 2018-06-09
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-4396-346x

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