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Alakpa, Enateri V.
Publications (3 of 3) Show all publications
Waddell, S. J., de Andrés, M. C., Tsimbouri, P. M., Alakpa, E. V., Cusack, M., Dalby, M. J. & Oreffo, R. O. C. (2018). Biomimetic oyster shell-replicated topography alters the behaviour of human skeletal stem cells. Journal of Tissue Engineering, 9, Article ID 2041731418794007.
Open this publication in new window or tab >>Biomimetic oyster shell-replicated topography alters the behaviour of human skeletal stem cells
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2018 (English)In: Journal of Tissue Engineering, ISSN 2041-7314, E-ISSN 2041-7314, Vol. 9, article id 2041731418794007Article in journal (Refereed) Published
Abstract [en]

The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from Pinctada maxima shells is known to enhance bone formation. However, to date, there is a paucity of information on the role of the topography of P. maxima surfaces, nacre and prism. To investigate this, nacre and prism topographical features were replicated onto polycaprolactone and skeletal stem cell behaviour on the surfaces studied. Skeletal stem cells on nacre surfaces exhibited an increase in cell area, increase in expression of osteogenic markers ALP (p<0.05) and OCN (p<0.01) and increased metabolite intensity (p<0.05), indicating a role of nacre surface to induce osteogenic differentiation, while on prism surfaces, skeletal stem cells did not show alterations in cell area or osteogenic marker expression and a decrease in metabolite intensity (p<0.05), demonstrating a distinct role for the prism surface, with the potential to maintain the skeletal stem cell phenotype.

Place, publisher, year, edition, pages
Sage Publications, 2018
Keywords
Nacre, topography, skeletal stem cell, osteogenic differentiation, bone regeneration
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Cell and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-152207 (URN)10.1177/2041731418794007 (DOI)000443733700001 ()30202512 (PubMedID)
Available from: 2018-10-31 Created: 2018-10-31 Last updated: 2018-10-31Bibliographically approved
Alakpa, E. V., Saeed, A., Chung, P., Riehle, M. O., Gadegaard, N., Dalby, M. J. & Cusack, M. (2018). The Prismatic Topography of Pinctada maxima Shell Retains Stem Cell Multipotency and Plasticity In Vitro. Advanced Biosystems, 2(6), Article ID 1800012.
Open this publication in new window or tab >>The Prismatic Topography of Pinctada maxima Shell Retains Stem Cell Multipotency and Plasticity In Vitro
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2018 (English)In: Advanced Biosystems, ISSN 2366-7478, Vol. 2, no 6, article id 1800012Article in journal (Refereed) Published
Abstract [en]

The shell of the bivalve mollusc Pinctada maxima is composed of the calcium carbonate polymorphs calcite and aragonite (nacre). Mother-of-pearl, or nacre, induces vertebrate cells to undergo osteogenesis and has good osteointegrative qualities in vivo. The calcite counterpart, however, is less researched in terms of the response of vertebrate cells. This study shows that isolation of calcite surface topography from the inherent chemistry allows viable long-term culture of bone marrow derived mesenchymal stem cells (MSCs). Self-renewal is evident from the increased gene expression of the self-renewal markers CD63, CD166, and CD271 indicating that cells cultured on the calcite topography maintain their stem cell phenotype. MSCs also retain their multipotency and can undergo successful differentiation into osteoblasts and adipocytes. When directed to adipogenesis, MSCs cultured on prism replicas are more amenable to differentiation than MSCs cultured on tissue culture polystyrene indicating a higher degree of plasticity in MSCs growing on calcite P. maxima prismatic topography. The study highlights the potential of the calcite topography of P. maxima as a biomimetic design for supporting expansion of MSC populations in vitro, which is of fundamental importance if it meets the demands for autologous MSCs for therapeutic use.

Place, publisher, year, edition, pages
WILEY-V C H VERLAG GMBH, 2018
Keywords
biominerals, calcite, self-renewal, stem cells, topography
National Category
Cell Biology
Identifiers
urn:nbn:se:umu:diva-153145 (URN)10.1002/adbi.201800012 (DOI)000446970600004 ()
Available from: 2018-11-07 Created: 2018-11-07 Last updated: 2018-11-07Bibliographically approved
Alakpa, E. V., Jayawarna, V., Burgess, K. E. V., West, C. C., Peault, B., Ulijn, R. V. & Dalby, M. J. (2017). Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels. Scientific Reports, 7, Article ID 6895.
Open this publication in new window or tab >>Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels
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2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, article id 6895Article in journal (Refereed) Published
Abstract [en]

Differentiation of stem cells to chondrocytes in vitro usually results in a heterogeneous phenotype. This is evident in the often detected over expression of type X collagen which, in hyaline cartilage structure is not characteristic of the mid-zone but of the deep-zone ossifying tissue. Methods to better match cartilage developed in vitro to characteristic in vivo features are therefore highly desirable in regenerative medicine. This study compares phenotype characteristics between pericytes, obtained from human adipose tissue, differentiated using diphenylalanine/serine (F2/S) peptide hydrogels with the more widely used chemical induced method for chondrogenesis. Significantly higher levels of type II collagen were noted when pericytes undergo chondrogenesis in the hydrogel in the absence of induction media. There is also a balanced expression of collagen relative to aggrecan production, a feature which was biased toward collagen production when cells were cultured with induction media. Lastly, metabolic profiles of each system show considerable overlap between both differentiation methods but subtle differences which potentially give rise to their resultant phenotype can be ascertained. The study highlights how material and chemical alterations in the cellular microenvironment have wide ranging effects on resultant tissue type.

Place, publisher, year, edition, pages
Nature Publishing Group, 2017
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-145813 (URN)10.1038/s41598-017-07255-z (DOI)000425969300028 ()28761049 (PubMedID)
Available from: 2018-03-22 Created: 2018-03-22 Last updated: 2018-06-09Bibliographically approved
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