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Hernandez-Verdeja, Tamara
Publications (4 of 4) Show all publications
Catalá, R., Carrasco-López, C., Perea-Resa, C., Hernández-Verdeja, T. & Salinas, J. (2019). Emerging Roles of LSM Complexes in Posttranscriptional Regulation of Plant Response to Abiotic Stress. Frontiers in Plant Science, 10, Article ID 167.
Open this publication in new window or tab >>Emerging Roles of LSM Complexes in Posttranscriptional Regulation of Plant Response to Abiotic Stress
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2019 (English)In: Frontiers in Plant Science, ISSN 1664-462X, E-ISSN 1664-462X, Vol. 10, article id 167Article, review/survey (Refereed) Published
Abstract [en]

It has long been assumed that the wide reprogramming of gene expression that modulates plant response to unfavorable environmental conditions is mainly controlled at the transcriptional level. A growing body of evidence, however, indicates that posttranscriptional regulatory mechanisms also play a relevant role in this control. Thus, the LSMs, a family of proteins involved in mRNA metabolism highly conserved in eukaryotes, have emerged as prominent regulators of plant tolerance to abiotic stress. Arabidopsis contains two main LSM ring-shaped heteroheptameric complexes, LSM1-7 and LSM2-8, with different subcellular localization and function. The LSM1-7 ring is part of the cytoplasmic decapping complex that regulates mRNA stability. On the other hand, the LSM2-8 complex accumulates in the nucleus to ensure appropriate levels of U6 snRNA and, therefore, correct pre-mRNA splicing. Recent studies reported unexpected results that led to a fundamental change in the assumed consideration that LSM complexes are mere components of the mRNA decapping and splicing cellular machineries. Indeed, these data have demonstrated that LSM1-7 and LSM2-8 rings operate in Arabidopsis by selecting specific RNA targets, depending on the environmental conditions. This specificity allows them to actively imposing particular gene expression patterns that fine-tune plant responses to abiotic stresses. In this review, we will summarize current and past knowledge on the role of LSM rings in modulating plant physiology, with special focus on their function in abiotic stress responses.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2019
Keywords
LSM complexes, abiotic stress responses, Arabidopsis, posttranscriptional regulation, mRNA decapping, pre-mRNA splicing
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-162505 (URN)10.3389/fpls.2019.00167 (DOI)000458949100001 ()30873189 (PubMedID)
Available from: 2019-08-21 Created: 2019-08-21 Last updated: 2019-08-21Bibliographically approved
Guinea Diaz, M., Hernandez-Verdeja, T., Kremnev, D., Crawford, T., Dubreuil, C. & Strand, Å. (2018). Redox regulation of PEP activity during seedling establishment in Arabidopsis thaliana. Nature Communications, 9, Article ID 50.
Open this publication in new window or tab >>Redox regulation of PEP activity during seedling establishment in Arabidopsis thaliana
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2018 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 9, article id 50Article in journal (Refereed) Published
Abstract [en]

Activation of the plastid-encoded RNA polymerase is tightly controlled and involves a network of phosphorylation and, as yet unidentified, thiol-mediated events. Here, we characterize PLASTID REDOX INSENSITIVE2, a redox-regulated protein required for full PEP-driven transcription. PRIN2 dimers can be reduced into the active monomeric form by thioredoxins through reduction of a disulfide bond. Exposure to light increases the ratio between the monomeric and dimeric forms of PRIN2. Complementation of prin2-2 with different PRIN2 protein variants demonstrates that the monomer is required for light-activated PEP-dependent transcription and that expression of the nuclear-encoded photosynthesis genes is linked to the activity of PEP. Activation of PEP during chloroplast development likely is the source of a retrograde signal that promotes nuclear LHCB expression. Thus, regulation of PRIN2 is the thiol-mediated mechanism required for full PEP activity, with PRIN2 monomerization via reduction by TRXs providing a mechanistic link between photosynthetic electron transport and activation of photosynthetic gene expression.

National Category
Other Biological Topics
Identifiers
urn:nbn:se:umu:diva-144086 (URN)10.1038/s41467-017-02468-2 (DOI)000419308300001 ()29298981 (PubMedID)
Available from: 2018-01-29 Created: 2018-01-29 Last updated: 2018-06-09Bibliographically approved
Hernandez-Verdeja, T. & Strand, Å. (2018). Retrograde Signals Navigate the Path to Chloroplast Development. Plant Physiology, 176(2), 967-976
Open this publication in new window or tab >>Retrograde Signals Navigate the Path to Chloroplast Development
2018 (English)In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 176, no 2, p. 967-976Article in journal (Refereed) Published
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-145152 (URN)10.1104/pp.17.01299 (DOI)000424285500002 ()29254985 (PubMedID)
Available from: 2018-03-02 Created: 2018-03-02 Last updated: 2018-06-09Bibliographically approved
Carrasco-Lopez, C., Hernandez-Verdeja, T., Perea-Resa, C., Abia, D., Catala, R. & Salinas, J. (2017). Environment-dependent regulation of spliceosome activity by the LSM2-8 complex in Arabidopsis. Nucleic Acids Research, 45(12), 7416-7431
Open this publication in new window or tab >>Environment-dependent regulation of spliceosome activity by the LSM2-8 complex in Arabidopsis
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2017 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 45, no 12, p. 7416-7431Article in journal (Refereed) Published
Abstract [en]

Spliceosome activity is tightly regulated to ensure adequate splicing in response to internal and external cues. It has been suggested that core components of the spliceosome, such as the snRNPs, would participate in the control of its activity. The experimental indications supporting this proposition, however, remain scarce, and the operating mechanisms poorly understood. Here, we present genetic and molecular evidence demonstrating that the LSM2-8 complex, the protein moiety of the U6 snRNP, regulates the spliceosome activity in Arabidopsis, and that this regulation is controlled by the environmental conditions. Our results show that the complex ensures the efficiency and accuracy of constitutive and alternative splicing of selected pre-mRNAs, depending on the conditions. Moreover, miss-splicing of most targeted pre-mRNAs leads to the generation of nonsense mediated decay signatures, indicating that the LSM2-8 complex also guarantees adequate levels of the corresponding functional transcripts. Interestingly, the selective role of the complex has relevant physiological implications since it is required for adequate plant adaptation to abiotic stresses. These findings unveil an unanticipated function for the LSM2-8 complex that represents a new layer of posttranscriptional regulation in response to external stimuli in eukaryotes.

Place, publisher, year, edition, pages
Oxford University Press, 2017
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-138541 (URN)10.1093/nar/gkx375 (DOI)000404879000041 ()
Available from: 2017-09-15 Created: 2017-09-15 Last updated: 2018-06-09Bibliographically approved
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