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Crawford, Tim
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Guinea Diaz, M., Hernandez-Verdeja, T., Kremnev, D., Crawford, T., Dubreuil, C. & Strand, Å. (2018). Redox regulation of PEP activity during seedling establishment in Arabidopsis thaliana. Nature Communications, 9, Article ID 50.
Open this publication in new window or tab >>Redox regulation of PEP activity during seedling establishment in Arabidopsis thaliana
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2018 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 9, article id 50Article in journal (Refereed) Published
Abstract [en]

Activation of the plastid-encoded RNA polymerase is tightly controlled and involves a network of phosphorylation and, as yet unidentified, thiol-mediated events. Here, we characterize PLASTID REDOX INSENSITIVE2, a redox-regulated protein required for full PEP-driven transcription. PRIN2 dimers can be reduced into the active monomeric form by thioredoxins through reduction of a disulfide bond. Exposure to light increases the ratio between the monomeric and dimeric forms of PRIN2. Complementation of prin2-2 with different PRIN2 protein variants demonstrates that the monomer is required for light-activated PEP-dependent transcription and that expression of the nuclear-encoded photosynthesis genes is linked to the activity of PEP. Activation of PEP during chloroplast development likely is the source of a retrograde signal that promotes nuclear LHCB expression. Thus, regulation of PRIN2 is the thiol-mediated mechanism required for full PEP activity, with PRIN2 monomerization via reduction by TRXs providing a mechanistic link between photosynthetic electron transport and activation of photosynthetic gene expression.

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urn:nbn:se:umu:diva-144086 (URN)10.1038/s41467-017-02468-2 (DOI)000419308300001 ()29298981 (PubMedID)
Available from: 2018-01-29 Created: 2018-01-29 Last updated: 2018-06-09Bibliographically approved
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