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Publications (10 of 14) Show all publications
Schindele, A., Holm, A., Kraft, S., Nylander, K., Allard, A. & Olofsson, K. (2025). Cross-evaluating Epstein-Barr virus, human papilloma virus, human cytomegalovirus and human adenovirus in nasal polyps and turbinate mucosa. Acta Oto-Laryngologica, 145(2), 164-167
Open this publication in new window or tab >>Cross-evaluating Epstein-Barr virus, human papilloma virus, human cytomegalovirus and human adenovirus in nasal polyps and turbinate mucosa
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2025 (English)In: Acta Oto-Laryngologica, ISSN 0001-6489, E-ISSN 1651-2251, Vol. 145, no 2, p. 164-167Article in journal (Refereed) Published
Abstract [en]

Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a common disease in which inflammatory responses to exogenic stressors, such as viral infections, has been recognised. The role of viruses in CRSwNP pathogenesis is unclear.

Aims/objectives: We aimed to characterise Epstein-Barr virus (EBV), human papillomavirus (HPV), human cytomegalovirus (HCMV), and human adenovirus (HAdV) in nasal polyps and adjacent paired healthy turbinate mucosa.

Materials and methods: We used real-time PCR for EBV, HCMV, and HAdV DNA detection, combined PCR/microarrays for HPV detection and genotyping, in samples from 45 patients with CRSwNP. Additionally, we used EBER in situ hybridisation for EBV detection.

Results: EBV detection with EBER-ISH was significantly higher in polyps (36%) versus turbinate mucosa (12%). None of the viral comparisons with PCR between polyps and turbinate mucosa for EBV-, HCMV- or HAdV-DNA showed statistically significant differences. All samples were HPV negative.

Conclusions and significance: We report higher expression of EBV in nasal polyps (36%) than in adjacent healthy turbinate mucosa (12%), using a valid method; EBER-ISH in 45 patients with CRSwNP. EBV might be a possible stressor that can trigger polypoid inflammation.

Place, publisher, year, edition, pages
Taylor & Francis, 2025
Keywords
Chronic rhinosinusitis with nasal polyps, EBER-ISH, Epstein-Barr virus, HAdV, HCMV, HPV, nasal mucosa
National Category
Microbiology in the medical area Otorhinolaryngology
Identifiers
urn:nbn:se:umu:diva-233990 (URN)10.1080/00016489.2024.2445025 (DOI)001387611900001 ()39921355 (PubMedID)2-s2.0-85214259351 (Scopus ID)
Funder
Cancerforskningsfonden i NorrlandRegion Jämtland HärjedalenRegion Västerbotten
Available from: 2025-01-14 Created: 2025-01-14 Last updated: 2025-05-27Bibliographically approved
Erlandsson, A., Werner, M., Holm, A., Schindele, A. & Olofsson, K. (2023). Balloon dilatation versus CO2 laser surgery in subglottic stenosis: a retrospective analysis of therapeutic approaches. Acta Oto-Laryngologica, 143(6), 528-535
Open this publication in new window or tab >>Balloon dilatation versus CO2 laser surgery in subglottic stenosis: a retrospective analysis of therapeutic approaches
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2023 (English)In: Acta Oto-Laryngologica, ISSN 0001-6489, E-ISSN 1651-2251, Vol. 143, no 6, p. 528-535Article in journal (Refereed) Published
Abstract [en]

Background: Subglottic stenosis (SGS) is a narrowing of the airway just below the vocal folds. The cause of SGS and the optimal care for these patients, have remained elusive. Endoscopic surgery of SGS using either balloon or CO2 laser is associated with recurrence.

Aims and objectives: Our aim is to compare surgery free intervals (SFI) between these two methods applied in two different timeframes. The knowledge gained from this project can support decision-making regarding surgical method choice.

Material and methods: Participants were retrospectively identified using medical records between 1999–2021. We used pre-defined broad inclusion criteria to identify cases using the International Classification of Disease (ICD-10). Primary outcome was surgery free intervals.

Results: 141 patients were identified, 63 met the criteria for SGS, and were included in the analysis. Results show no significant difference in SFI, comparing balloon dilatation and CO2 laser.

Conclusion: These findings demonstrate no detected difference in treatment intervals (SFI) when comparing these two commonly used surgical alternatives for SGS.

Significance: The outcome of this report supports surgical freedom of choice based on the surgeon’s experience and skill and ushes for further studies on patient experience regarding these two therapeutic approaches.

Place, publisher, year, edition, pages
Taylor & Francis, 2023
Keywords
balloon dilatation, CO2 laser, idiopathic subglottic stenosis, Subglottic stenosis, surgery free intervals
National Category
Otorhinolaryngology Surgery
Identifiers
urn:nbn:se:umu:diva-212260 (URN)10.1080/00016489.2023.2222756 (DOI)001011786800001 ()37343275 (PubMedID)2-s2.0-85164105528 (Scopus ID)
Funder
Cancerforskningsfonden i Norrland, AMP 18-925Cancerforskningsfonden i Norrland, AMP-19-973Region Västerbotten, RV 967352Region Västerbotten, RV 979607
Available from: 2023-07-20 Created: 2023-07-20 Last updated: 2023-07-20Bibliographically approved
Schindele, A., Holm, A., Nylander, K., Allard, A. & Olofsson, K. (2022). Mapping human papillomavirus, Epstein–Barr virus, cytomegalovirus, adenovirus, and p16 in laryngeal cancer. Discover Oncology, 13(1), Article ID 18.
Open this publication in new window or tab >>Mapping human papillomavirus, Epstein–Barr virus, cytomegalovirus, adenovirus, and p16 in laryngeal cancer
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2022 (English)In: Discover Oncology, E-ISSN 2730-6011, Vol. 13, no 1, article id 18Article in journal (Refereed) Published
Abstract [en]

Purpose: Apart from tobacco and alcohol, viral infections are proposed as risk factors for laryngeal cancer. The occurrence of oncogenic viruses including human papilloma virus (HPV) and Epstein–Barr virus (EBV), in laryngeal squamous cell carcinoma (LSCC) varies in the world. Carcinogenesis is a multi-step process, and the role of viruses in LSCC progression has not been clarified. We aimed to analyze the presence and co-expression of HPV, EBV, human cytomegalovirus (HCMV) and human adenovirus (HAdV) in LSCC. We also investigated if p16 can act as surrogate marker for HPV in LSCC.

Methods: Combined PCR/microarrays (PapilloCheck®) were used for detection and genotyping of HPV DNA, real-time PCR for EBV, HCMV and HAdV DNA detection, and EBER in situ hybridization (EBER-ISH) for EBV detection in tissue from 78 LSCC patients. Additionally, we analyzed p16 expression with immunohistochemistry.

Results: Thirty-three percent (26/78) of LSCC tumor samples were EBV positive, 9% (7/78) HCMV positive and 4% (3/78) HAdV positive. Due to DNA fragmentation, 45 samples could not be analyzed with PapilloCheck®; 9% of the remaining (3/33) were high-risk HPV16 positive and also over-expressed p16. A total of 14% (11/78) of the samples over-expressed p16.

Conclusion: These findings present a mapping of HPV, EBV, HCMV and HAdV, including the HPV surrogate marker p16, in LSCC in this cohort. Except for EBV, which was detected in a third of the samples, data show viral infection to be uncommon, and that p16 does not appear to be a specific surrogate marker for high-risk HPV infection in LSCC.

Place, publisher, year, edition, pages
Springer, 2022
National Category
Cancer and Oncology Otorhinolaryngology
Research subject
Oncology
Identifiers
urn:nbn:se:umu:diva-193581 (URN)10.1007/s12672-022-00475-4 (DOI)000771496000002 ()35312853 (PubMedID)2-s2.0-85126886934 (Scopus ID)
Available from: 2022-04-19 Created: 2022-04-19 Last updated: 2022-10-12Bibliographically approved
Dernstedt, A., Leidig, J., Holm, A., Kerkman, P., Mjösberg, J., Ahlm, C., . . . Forsell, M. N. E. (2021). Regulation of Decay Accelerating Factor Primes Human Germinal Center B Cells for Phagocytosis. Frontiers in Immunology, 11, Article ID 599647.
Open this publication in new window or tab >>Regulation of Decay Accelerating Factor Primes Human Germinal Center B Cells for Phagocytosis
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2021 (English)In: Frontiers in Immunology, E-ISSN 1664-3224, Vol. 11, article id 599647Article in journal (Refereed) Published
Abstract [en]

Germinal centers (GC) are sites for extensive B cell proliferation and homeostasis is maintained by programmed cell death. The complement regulatory protein Decay Accelerating Factor (DAF) blocks complement deposition on host cells and therefore also phagocytosis of cells. Here, we show that B cells downregulate DAF upon BCR engagement and that T cell-dependent stimuli preferentially led to activation of DAF(lo) B cells. Consistent with this, a majority of light and dark zone GC B cells were DAF(lo) and susceptible to complement-dependent phagocytosis, as compared with DAF(hi) GC B cells. We could also show that the DAF(hi) GC B cell subset had increased expression of the plasma cell marker Blimp-1. DAF expression was also modulated during B cell hematopoiesis in the human bone marrow. Collectively, our results reveal a novel role of DAF to pre-prime activated human B cells for phagocytosis prior to apoptosis.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2021
Keywords
human B cell development, germinal center (GC), decay accelerating factor (DAF), complement-mediated phagocytosis, complement regulating proteins
National Category
Immunology in the medical area
Identifiers
urn:nbn:se:umu:diva-179577 (URN)10.3389/fimmu.2020.599647 (DOI)000608470700001 ()33469456 (PubMedID)2-s2.0-85099651060 (Scopus ID)
Funder
NIH (National Institute of Health), U19AI142777-01Swedish Research Council, 2016-06598
Available from: 2021-02-04 Created: 2021-02-04 Last updated: 2025-02-24Bibliographically approved
Holm, A., Allard, A., Eriksson, I., Laurell, G., Nylander, K. & Olofsson, K. (2020). Absence de papillomavirus humain à risque élevé dans le papillome inversé naso-sinusien p16 positif: [Absence of high-risk human papillomavirus in p16 positive inverted sinonasal papilloma]. Annales Francaises d'Oto-Rhino-Laryngologie et de Pathologie Cervico-Faciale, 137(3), 186-191
Open this publication in new window or tab >>Absence de papillomavirus humain à risque élevé dans le papillome inversé naso-sinusien p16 positif: [Absence of high-risk human papillomavirus in p16 positive inverted sinonasal papilloma]
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2020 (French)In: Annales Francaises d'Oto-Rhino-Laryngologie et de Pathologie Cervico-Faciale, ISSN 1879-7261, Vol. 137, no 3, p. 186-191Article in journal (Refereed) Published
Abstract [fr]

Le papillome inversé naso-sinusien (PINS) est une tumeur relativement rare dont l’étiologie est mal connue. Elle se caractérise par une agressivité locale et un fort potentiel de récidive en dépit d’une histologie bénigne.

Objectif: L’objectif de cette étude était d’identifier la présence du papillomavirus humain (HPV) et de son marqueur de substitution, la protéine p16, dans des prélèvements tissulaires de PINS issus d’une cohorte régionale.

Matériels et méthodes: À partir de notre cohorte régionale de 88 patients atteints de PINS traités entre 1984 et 2014, 54 sujets ont été sélectionnés et inclus dans cette étude. La technologie PCR a été réalisée sur 53 prélèvements et la coloration immunohistochimique pour recherche de p16 a été réalisée sur 54 prélèvements. L’ADN a été extrait après confirmation histopathologique du PINS. Un génotypage pour 13 types de HPV à risque élevé, 5 types de HPV à risque oncogène et 6 types de HPV à faible risque a été réalisé à l’aide du test de dépistage HPV PapilloCheck®.

Résultats: L’analyse HPV a été réalisable sur 38 des 53 prélèvements. Sur ces 38 prélèvements, seuls 2 étaient positifs pour HPV 11. L’analyse immunohistochimique a montré que p16 était présent dans l’épithélium de tous les prélèvements, et dans les régions papillomateuses de 37 prélèvements.

Conclusion: Étant donné que seuls 2 sur 38 PINS étaient positifs pour HPV (type 11) et que, dans le même temps, p16 était positif dans l’épithélium de tous les prélèvements et dans 37 des 38 régions papillomateuses, nous avons conclu que p16 ne peut pas être utilisé comme marqueur de substitution pour l’infection HPV à risque élevé dans le PINS. Nous préparons actuellement une étude multicentrique prospective afin d’augmenter la puissance de l’étude et de pouvoir mieux évaluer les implications cliniques de HPV et de p16 dans le PINS.

Place, publisher, year, edition, pages
Elsevier, 2020
National Category
Ophthalmology
Identifiers
urn:nbn:se:umu:diva-197931 (URN)10.1016/j.aforl.2019.10.004 (DOI)2-s2.0-85075428840 (Scopus ID)
Available from: 2022-07-08 Created: 2022-07-08 Last updated: 2022-07-08Bibliographically approved
Holm, A., Allard, A., Eriksson, I., Laurell, G., Nylander, K. & Olofsson, K. (2020). Absence of high-risk human papilloma virus in p16 positive inverted sinonasal papilloma. European Annals of Otorhinolaryngology, Head and Neck Diseases, 137(3), 201-206
Open this publication in new window or tab >>Absence of high-risk human papilloma virus in p16 positive inverted sinonasal papilloma
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2020 (English)In: European Annals of Otorhinolaryngology, Head and Neck Diseases, ISSN 1879-7296, Vol. 137, no 3, p. 201-206Article in journal (Refereed) Published
Abstract [en]

Objectives: Sinonasal inverted papilloma (SIP) is a relatively rare disease, and its etiology is not understood. It is characterized by locally aggressive growth and a strong tendency to recur despite its benign histology.

Aims: The aim of this study was to identify the presence of human papilloma virus (HPV) and its surrogate marker p16 in SIP tissue samples from a regional cohort.

Material and methods: Subjects were identified from our regional center cohort of 88 SIP patients treated between 1984–2014. From these subjects, 54 were included in this study. Of these, 53 biopsies were analyzed with PCR, and 54 samples were immunohistochemically stained for p16. DNA was extracted from histopathologically verified SIP. Genotype screening for 13 high risk-, 5 oncogenic and 6 low risk HPV types was performed using the PapilloCheck® HPV-screening test.

Results: HPV analysis was successful for 38 of 53 samples. Of the 38 successfully analyzed samples, only 2 samples were positive for HPV 11. Notably, p16 was present in the epithelia in all samples, and in the papilloma lesions in 37 samples.

Conclusion: Since only 2 out of 38 SIPs were positive for HPV (type 11), and at the same time p16 was positive in epithelia in all samples and in 37 of 38 papilloma lesions of the samples, it is concluded that p16 cannot be used as a surrogate marker for high-risk HPV-infection in SIP. We are currently planning a prospective, multicenter study in order to increase the study power and in order to be able to better evaluate the clinical implications of HPV-and p16 in SIP.

Place, publisher, year, edition, pages
Elsevier Masson SAS, 2020
Keywords
Human papilloma virus, Inverted nasal papilloma, PapilloCheck®, Immunohistochemistry
National Category
Otorhinolaryngology
Research subject
Oto-Rhino-Laryngology
Identifiers
urn:nbn:se:umu:diva-158487 (URN)10.1016/j.anorl.2017.10.008 (DOI)000534479000011 ()31732387 (PubMedID)2-s2.0-85075518918 (Scopus ID)
Note

Originally included in thesis in manuscript form.

Available from: 2019-04-29 Created: 2019-04-29 Last updated: 2024-07-02Bibliographically approved
Schindele, A., Holm, A., Nylander, K., Allard, A. & Olofsson, K. (2020). Low Epstein-Barr virus count in sinonasal inverted papilloma. Acta Oto-Laryngologica, 140(5), 413-417
Open this publication in new window or tab >>Low Epstein-Barr virus count in sinonasal inverted papilloma
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2020 (English)In: Acta Oto-Laryngologica, ISSN 0001-6489, E-ISSN 1651-2251, Vol. 140, no 5, p. 413-417Article in journal (Refereed) Published
Abstract [en]

Background: Sinonasal inverted papilloma (SIP) is a benign tumour originating from the sinonasal mucosa showing an extensive growth pattern, a high risk of recurrence and a 5–10% risk to malignify. Epstein-Barr virus (EBV) is an oncogenic herpesvirus which infects most individuals via the saliva eliciting a latent infection. Previous studies have been reporting variable data on EBV in SIP, and there is no present appreciation regarding the association between these.

Aims/objectives: The aims were to investigate the presence and count of EBV in SIP and map the viral distribution in the epithelium versus the connective tissue.

Material and method: Fifty-three SIP patients were identified in the Pathology Department register at the University Hospital of Umeå. The biopsies were analysed with Epstein-Barr Encoded Region (EBER) in situ hybridization. EBER-positive cells were counted in the epithelium and connective tissue.

Results: We found EBER-stained cells in 30% of the cases, where 19% of these had an abundance of stained cells, and the rest showed a low count.

Conclusions/significance: These findings demonstrate a low EBV count in SIP. EBV is less likely to be a causative agent in the formation of SIP, or its malignant transformation.

Place, publisher, year, edition, pages
Taylor & Francis, 2020
Keywords
Epstein-Barr virus, sinonasal inverted papilloma, EBER-ISH
National Category
Otorhinolaryngology
Identifiers
urn:nbn:se:umu:diva-168828 (URN)10.1080/00016489.2020.1724330 (DOI)000514736300001 ()32068495 (PubMedID)2-s2.0-85079719070 (Scopus ID)
Funder
Region Västerbotten
Available from: 2020-03-19 Created: 2020-03-19 Last updated: 2023-03-24Bibliographically approved
Holm, A. M. (2019). Human papillomavirus in sinonasal inverted papilloma, recurrent respiratory papilloma and non-malignant tonsils. (Doctoral dissertation). Umeå: Umeå universitet
Open this publication in new window or tab >>Human papillomavirus in sinonasal inverted papilloma, recurrent respiratory papilloma and non-malignant tonsils
2019 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Humant papillomvirus i respiratoriska papillom, inverterade näspapillom och vid godartad sjukdom i halsmandlarna
Abstract [en]

Background: Human papillomavirus (HPV) is known to cause recurrent respiratory papilloma (RRP) and certain types of oropharyngeal cancer. HPV has also been associated with sinonasal inverted papilloma (SIP). HPV transmission routes are under investigation and the conviction is that the infection occurs sexually at an adult stage, however, vertical transmission at birth with a dormant viral condition until disease eruption/co-activation has been stated as a possibility.

Purpose: The purpose of this work was to contribute to the understanding of HPV related chronic diseases in the airway. Specific aims were: 1. To increase understanding regarding changes in the immune system as well as of the glycosaminoglycan hyaluronan in patients with RRP. 2. To evaluate prevalence of HPV and its surrogate marker p16 in SIP as well as HPV, p16 and Epstein-Barr virus (EBV) in benign tonsillar disease. HPV and EBV in non-malignant tonsillar disease were studied due to the fact that incidence of HPV positive tonsillar cancer is increasing and the time of viral infection is unknown.

Methods: A phenotypic characterization of peripheral blood from 16 RRP patients and 12 age-matched controls, using immunoflow cytometry, and monoclonal antibodies against differentiation and activation markers, was performed. The cytokine mRNA profile of monocytes, T helper-, T cytotoxic-, and NK cells was assessed using RT-qPCR. 54 SIP samples were studied of which 53 were available for analyzation with PCR. Genotype screening for 18 high risk and six low risk HPV types was performed using the PapilloCheck® HPV-screening test (a PCR method). 54 samples were immunohistochemically (IHC) stained for p16. Biopsies from vocal folds (VFs) and false vocal folds (FVFs) were collected from 24 patients with RRP, 12 were randomly selected to histochemistry for Hyaluronan (HA) and IHC staining for CD44 in the epithelium, stroma and RRP lesions. The remaining 12 patients were analyzed for HA molecular mass distribution with a gas-phase electrophoretic molecular mobility analyzer (GEMMA). Eight VF samples and four FVF samples were successfully analyzed. Biopsies from 40 non-malignant tonsils were analyzed using Papillocheck® for HPV, IHC for p16 and EBER analysis for EBV.

Results: We found a dominance of cytotoxic T cells, activated NK cells, and high numbers of stressed MIC A/B (MHC class I chain-related molecule A/B) expressing lymphocytes. The HPV analysis was successful for 38 SIP samples and two (5%) were positive for HPV 11. Notably, p16 was present in the epithelia of all samples and in the papilloma portions in 37 of 38 samples. We found extensive HA staining in the stroma of both VFs and FVFs. CD44 was expressed throughout the epithelium, stroma, and RRP lesions in both FVFs and VFs, it did however, not concur with the expression of HA. Very high mass HA was found in both VFs and FVFs, though more variation regarding amounts of HA was seen in the VFs compared to FVFs. No HPV was found in non-malignant tonsils, the p16 levels were low and the counted EBER positive cells showed great variation in numbers.

Conclusions: Our findings demonstrate an immune dysregulation with inverted CD4+/CD8+ ratio and aberrant cytokine mRNA production in RRP patients, compared to healthy controls. We concluded that p16 cannot be used as a surrogate marker for high-risk HPV-infection in SIP and that HPV incidence was low (5%). CD44 does not seem to bind to HA, which might explain the noninflammatory response previously described in RRP. Very high mass HA possibly crosslinked was seen in both VFs and FVFs. A possibility to counteract inflammatory crosslinking of HA may be found for medical treatment options in RRP.

Place, publisher, year, edition, pages
Umeå: Umeå universitet, 2019. p. 56
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 1955
Keywords
Human papillomavirus, recurrent respiratory papillomatosis, sinonasal inverted papilloma, non-malignant tonsillar disease, Epstein-Barr virus, immune system, p16, Hyaluronan
National Category
Otorhinolaryngology
Research subject
Oto-Rhino-Laryngology
Identifiers
urn:nbn:se:umu:diva-158489 (URN)978-91-7601-865-1 (ISBN)
Public defence
2019-05-24, Sal D, Unod T9, Norrlands Universitetssjukhus, Umeå, 09:00 (English)
Opponent
Supervisors
Note

Titel enligt titelblad: Human papillomavirus in recurrent respiratory papilloma, sinonasal inverted papilloma, and non-malignant tonsils

Available from: 2019-05-03 Created: 2019-04-29 Last updated: 2024-07-02Bibliographically approved
Holm, A. (2019). Hyaluronan i respiratoriska papillom. Svensk ÖNH-tidskrift, 3, 13-14
Open this publication in new window or tab >>Hyaluronan i respiratoriska papillom
2019 (Swedish)In: Svensk ÖNH-tidskrift, ISSN 1400-0121, Vol. 3, p. 13-14Article in journal, Editorial material (Other academic) Published
Place, publisher, year, edition, pages
Taylor & Francis, 2019
National Category
Otorhinolaryngology
Research subject
Oto-Rhino-Laryngology
Identifiers
urn:nbn:se:umu:diva-196194 (URN)
Available from: 2022-06-09 Created: 2022-06-09 Last updated: 2022-06-30Bibliographically approved
Holm, A., Schindele, A., Allard, A., Eriksson, I., Sandström, K., Laurell, G., . . . Olofsson, K. (2019). Mapping of Human Papilloma Virus, p16, and Epstein-Barr Virusin Non-Malignant Tonsillar Disease. Laryngoscope Investigative Otolaryngology (LIO), 4(3), 285-291
Open this publication in new window or tab >>Mapping of Human Papilloma Virus, p16, and Epstein-Barr Virusin Non-Malignant Tonsillar Disease
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2019 (English)In: Laryngoscope Investigative Otolaryngology (LIO), E-ISSN 2378-8038, Vol. 4, no 3, p. 285-291Article in journal (Refereed) Published
Abstract [en]

Objectives: Due to their location in the entrance of the aero‐digestive tract, tonsils are steadily exposed to viruses. Human papilloma virus (HPV) and Epstein‐Barr virus (EBV) are two potentially oncogenic viruses that tonsils encounter. The incidence of HPV positive tonsillar cancer is on the rise and it is unknown when infection with HPV occurs.

Aim: To investigate if tonsils are infected with HPV and EBV, to study the co‐expression of HPV and its surrogate marker p16, and to evaluate the number of EBV positive cells in benign tonsillar disease.

Materials and Methods: Tonsils from 40 patients in a university hospital were removed due to hypertrophy, chronic or recurrent infection. These were analyzed for presence of HPV, its surrogate marker p16, and EBV. HPV was studied using PapilloCheck (a PCR method), while p16 was identified in epithelial and lymphoid tissue with immunohistochemistry and EBV using EBER‐ISH (Epstein‐Barr encoding region–in situ hybridization).

Results: HPV was not detected, and p16 was present at low numbers in all epithelial samples as well as in 92.5% of the lymphoid tonsillar samples. At least one EBER‐positive cell was seen in 65% of cases. Larger numbers of EBER‐expressing cells were only seen in two cases.

Conclusion: These findings demonstrate that EBV and HPV infect tonsils independently, but further studies are warranted to confirm their infectious relationship.

Level of Evidence: Cross‐sectional study

Place, publisher, year, edition, pages
Wiley-Blackwell, 2019
Keywords
Human papillomavirus, Epstein-Barr virus, non-malignant tonsillar disease, EBER-ISH, PapilloCheck, immunohistochemistry
National Category
Otorhinolaryngology
Research subject
Oto-Rhino-Laryngology
Identifiers
urn:nbn:se:umu:diva-158485 (URN)10.1002/lio2.260 (DOI)000471907200002 ()31236460 (PubMedID)
Funder
Västerbotten County Council
Available from: 2019-04-29 Created: 2019-04-29 Last updated: 2024-07-02Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-3522-1842

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