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Barange, Deepak Kumar
Publications (3 of 3) Show all publications
Doyle, S. M., Rigal, A., Grones, P., Karady, M., Barange, D. K., Majda, M., . . . Robert, S. (2019). A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN‐FORMED protein polarity and relocalisation in Arabidopsis. New Phytologist, 223(3), 1420-1432
Open this publication in new window or tab >>A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN‐FORMED protein polarity and relocalisation in Arabidopsis
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2019 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 223, no 3, p. 1420-1432Article in journal (Refereed) Published
Abstract [en]

Distribution of auxin within plant tissues is of great importance for developmental plasticity, including root gravitropic growth. Auxin flow is directed by the subcellular polar distribution and dynamic relocalisation of auxin transporters such as the PIN‐FORMED (PIN) efflux carriers, which can be influenced by the main natural plant auxin indole‐3‐acetic acid (IAA). Anthranilic acid (AA) is an important early precursor of IAA and previously published studies with AA analogues have suggested that AA may also regulate PIN localisation.

Using Arabidopsis thaliana as a model species, we studied an AA‐deficient mutant displaying agravitropic root growth, treated seedlings with AA and AA analogues and transformed lines to over‐produce AA while inhibiting its conversion to downstream IAA precursors.

We showed that AA rescues root gravitropic growth in the AA‐deficient mutant at concentrations that do not rescue IAA levels. Overproduction of AA affects root gravitropism without affecting IAA levels. Treatments with, or deficiency in, AA result in defects in PIN polarity and gravistimulus‐induced PIN relocalisation in root cells.

Our results revealed a previously unknown role for AA in the regulation of PIN subcellular localisation and dynamics involved in root gravitropism, which is independent of its better known role in IAA biosynthesis.

Place, publisher, year, edition, pages
John Wiley & Sons, 2019
Keywords
anthranilic acid (AA), Arabidopsis thaliana, auxin transport, PIN polarity, PIN-FORMED proteins, root gravitropism
National Category
Botany
Identifiers
urn:nbn:se:umu:diva-162321 (URN)10.1111/nph.15877 (DOI)000475918000032 ()31038751 (PubMedID)2-s2.0-85068258314 (Scopus ID)
Available from: 2019-08-19 Created: 2019-08-19 Last updated: 2023-03-24Bibliographically approved
Vain, T., Raggi, S., Ferro, N., Barange, D. K., Kieffer, M., Ma, Q., . . . Robert, S. (2019). Selective auxin agonists induce specific AUX/IAA protein degradation to modulate plant development. Proceedings of the National Academy of Sciences of the United States of America, 116(13), 6463-6472
Open this publication in new window or tab >>Selective auxin agonists induce specific AUX/IAA protein degradation to modulate plant development
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2019 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 116, no 13, p. 6463-6472Article in journal (Refereed) Published
Abstract [en]

The plant hormone auxin coordinates almost all aspects of plant development. Throughout plant life, the expression of hundreds of genes involved in auxin regulation is orchestrated via several combinatorial and cell-specific auxin perception systems. An effective approach to dissect these complex pathways is the use of synthetic molecules that target specific processes of auxin activity. Here, we describe synthetic auxins, RubNeddins (RNs), which act as selective auxin agonists. The RN with the greatest potential for dissecting auxin perception was RN4, which we used to reveal a role for the chromatin remodeling ATPase BRAHMA in apical hook development. Therefore, the understanding of RN mode of action paves the way to dissecting specific molecular components involved in auxin-regulated developmental processes.Auxin phytohormones control most aspects of plant development through a complex and interconnected signaling network. In the presence of auxin, AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors are targeted for degradation by the SKP1-CULLIN1-F-BOX (SCF) ubiquitin-protein ligases containing TRANSPORT INHIBITOR RESISTANT 1/AUXIN SIGNALING F-BOX (TIR1/AFB). CULLIN1-neddylation is required for SCFTIR1/AFB functionality, as exemplified by mutants deficient in the NEDD8-activating enzyme subunit AUXIN-RESISTANT 1 (AXR1). Here, we report a chemical biology screen that identifies small molecules requiring AXR1 to modulate plant development. We selected four molecules of interest, RubNeddin 1 to 4 (RN1 to -4), among which RN3 and RN4 trigger selective auxin responses at transcriptional, biochemical, and morphological levels. This selective activity is explained by their ability to consistently promote the interaction between TIR1 and a specific subset of AUX/IAA proteins, stimulating the degradation of particular AUX/IAA combinations. Finally, we performed a genetic screen using RN4, the RN with the greatest potential for dissecting auxin perception, which revealed that the chromatin remodeling ATPase BRAHMA is implicated in auxin-mediated apical hook development. These results demonstrate the power of selective auxin agonists to dissect auxin perception for plant developmental functions, as well as offering opportunities to discover new molecular players involved in auxin responses.

Place, publisher, year, edition, pages
Washington: Proceedings of the National Academy of Sciences of the United States of America (PNAS), 2019
Keywords
auxin, chemical biology, selective agonist, prohormone, hormone perception
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-157293 (URN)10.1073/pnas.1809037116 (DOI)000462382800094 ()30850516 (PubMedID)2-s2.0-85063945983 (Scopus ID)
Funder
Swedish Research Council, 2013-4632Swedish Research Council, 2016-00768VinnovaKnut and Alice Wallenberg FoundationStiftelsen Olle Engkvist ByggmästareThe Kempe FoundationsCarl Tryggers foundation NIH (National Institute of Health), NIH GM43644
Available from: 2019-03-14 Created: 2019-03-14 Last updated: 2023-03-24Bibliographically approved
Barange, D. K., Johnson, M. T., Cairns, A. G., Olsson, R. & Almqvist, F. (2016). Regio- and Stereoselective Alkylation of Pyridine-N-oxides: Synthesis of Substituted Piperidines and Pyridines. Organic Letters, 18(24), 6228-6231
Open this publication in new window or tab >>Regio- and Stereoselective Alkylation of Pyridine-N-oxides: Synthesis of Substituted Piperidines and Pyridines
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2016 (English)In: Organic Letters, ISSN 1523-7060, E-ISSN 1523-7052, Vol. 18, no 24, p. 6228-6231Article in journal (Refereed) Published
Abstract [en]

Regio- and stereoselective addition of alkyl Grignard reagents to pyridine-N-oxides gave C2-alkylated N-hydroxy-1,2,5,6-tetrahydropyridines and trans-2,3-disubstituted N-hydroxy-1,2,5,6-tetrahydropyridines in good to excellent yields. These intermediates were aromatized or alternatively reduced in one-pot methodologies for efficient syntheses of alkylpyridines or piperidines, respectively. These reactions have a broad substrate scope and short reaction times.

National Category
Organic Chemistry
Identifiers
urn:nbn:se:umu:diva-130226 (URN)10.1021/acs.orglett.6b02667 (DOI)000390180300004 ()2-s2.0-85006474723 (Scopus ID)
Available from: 2017-01-16 Created: 2017-01-14 Last updated: 2023-03-23Bibliographically approved
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