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Honkala, Emma
Publications (2 of 2) Show all publications
Olsson, J., Johansson, J., Honkala, E., Blomqvist, B., Kok, E., Weidung, B., . . . Elgh, F. (2019). Urea dilution of serum for reproducible anti-HSV1 IgG avidity index. BMC Infectious Diseases, 19, Article ID 164.
Open this publication in new window or tab >>Urea dilution of serum for reproducible anti-HSV1 IgG avidity index
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2019 (English)In: BMC Infectious Diseases, ISSN 1471-2334, E-ISSN 1471-2334, Vol. 19, article id 164Article in journal (Refereed) Published
Abstract [en]

Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content in human sera. Human serum from two distinct cohorts; one a biobank collection (Betula) (n = 28), and one from a clinical diagnostics laboratory at Northern Sweden University Hospital (NUS) (n = 18), were assessed for presence of IgG antibodies against HSV1 by a commercially available ELISA-kit. Addition of urea at the incubation step reduces effective binding, and the ratio between urea treated sample and non-treated sample was used to express an avidity index (AI) for individual samples. AI score ranged between 43.2 and 73.4% among anti-HSV1 positive biobank sera. Clinical samples ranged between 36.3 and 74.9%. Reproducibility expressed as an intraclass correlation coefficient (ICC) was estimated at 0.948 (95% CI: 0.900-0.979) and 0.989 (95% CI 0.969-0.996) in the biobank and clinical samples, respectively. The method allows for AI scoring of anti-HSV1 IgG from individual human sera with a single measurement. The least significant change between two measurements at the p < 0.05 level was estimated at 5.4 and 3.2 points, respectively, for the two assessed cohorts.

Place, publisher, year, edition, pages
BioMed Central, 2019
Herpes simplex, IgG, Avidity, ELISA, Primary infection, Reactivated infection
National Category
Infectious Medicine
urn:nbn:se:umu:diva-157213 (URN)10.1186/s12879-019-3769-x (DOI)000459030400003 ()30764767 (PubMedID)
Available from: 2019-03-25 Created: 2019-03-25 Last updated: 2019-03-25Bibliographically approved
Olsson, J., Lövheim, H., Honkala, E., Karhunen, P. J., Elgh, F. & Kok, E. H. (2016). HSV presence in brains of individuals without dementia: the TASTY brain series. Disease Models and Mechanisms, 9(11), 1349-1355
Open this publication in new window or tab >>HSV presence in brains of individuals without dementia: the TASTY brain series
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2016 (English)In: Disease Models and Mechanisms, ISSN 1754-8403, E-ISSN 1754-8411, Vol. 9, no 11, p. 1349-1355Article in journal (Refereed) Published
Abstract [en]

Herpes simplex virus (HSV) type 1 affects a majority of the population and recent evidence suggests involvement in Alzheimer's disease aetiology. We investigated the prevalence of HSV type 1 and 2 in the Tampere Autopsy Study (TASTY) brain samples using PCR and sero-positivity in plasma, and associations with Alzheimer's disease neuropathology. HSV was shown to be present in human brain tissue in 11/584 (1.9%) of samples in the TASTY cohort, of which six had Alzheimer's disease neuropathological amyloid beta (A beta) aggregations. Additionally, serological data revealed 86% of serum samples tested were IgG-positive for HSV. In conclusion, we report epidemiological evidence of the presence of HSV in brain tissue free from encephalitis symptoms in a cohort most closely representing the general population (a minimum prevalence of 1.9%). Whereas 6/11 samples with HSV DNA in the brain tissue had A beta aggregations, most of those with A beta aggregations did not have HSV present in the brain tissue.

Herpes simplex virus, Amyloid beta aggregations, Alzheimer's disease, PCR detection, Human brain tissue, Paraffin-embedded samples
National Category
Clinical Laboratory Medicine
urn:nbn:se:umu:diva-129884 (URN)10.1242/dmm.026674 (DOI)000387581000011 ()27664135 (PubMedID)
Available from: 2017-01-16 Created: 2017-01-10 Last updated: 2018-06-09Bibliographically approved

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