umu.sePublications
Change search
Link to record
Permanent link

Direct link
BETA
Lindholm, Mark
Publications (5 of 5) Show all publications
Johansson, A., Claesson, R., Höglund-Åberg, C., Haubek, D., Lindholm, M., Jasim, S. & Oscarsson, J. (2019). Genetic Profiling of Aggregatibacter actinomycetemcomitans Serotype B Isolated from Periodontitis Patients Living in Sweden. Pathogens, 8(3), 1-13, Article ID 153.
Open this publication in new window or tab >>Genetic Profiling of Aggregatibacter actinomycetemcomitans Serotype B Isolated from Periodontitis Patients Living in Sweden
Show others...
2019 (English)In: Pathogens, ISSN 2076-0817, Vol. 8, no 3, p. 1-13, article id 153Article in journal (Refereed) Published
Abstract [en]

The bacterium Aggregatibacter actinomycetemcomitans is associated with aggressive forms of periodontitis and with systemic diseases, such as endocarditis. By assessing a Ghanaian longitudinal adolescent cohort, we earlier recognized the cagE gene as a possible diagnostic marker for a subgroup of JP2 and non-JP2 genotype serotype b A. actinomycetemcomitans strains, associated with high leukotoxicity as determined in a semi-quantitative cell assay. This group of A. actinomycetemcomitans is associated with the progression of attachment loss. In the present work, we used conventional polymerase chain reaction (PCR) and quantitative PCR to perform the cagE genotyping of our collection of 116 selected serotype b A. actinomycetemcomitans strains, collected over a period of 15 years from periodontitis patients living in Sweden. The A. actinomycetemcomitans strains carrying cagE (referred to as cagE+; n = 49) were compared to the cagE-negative strains (n = 67), present at larger proportions in the subgingival plaque samples, and were also much more prevalent in the young (≤35 years) compared to in the old (>35 years) group of patients. Our present results underline the potential use of cagE genotyping in the risk assessment of the development of periodontal attachment loss in Swedish adolescents.

Place, publisher, year, edition, pages
Basel, Switzerland: MDPI, 2019
Keywords
Aggregatibacter actinomycetemcomitans; cagE; virB1; virB4; genotype; virulence
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Infectious Diseases
Identifiers
urn:nbn:se:umu:diva-163485 (URN)10.3390/pathogens8030153 (DOI)000488003600042 ()31533208 (PubMedID)
Available from: 2019-09-23 Created: 2019-09-23 Last updated: 2019-10-24Bibliographically approved
Lindholm, M., Aung, K. M., Wai, S. N. & Oscarsson, J. (2019). Role of OmpA1 and OmpA2 in Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus serum resistance. Journal of Oral Microbiology, 11(1), Article ID 1536192.
Open this publication in new window or tab >>Role of OmpA1 and OmpA2 in Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus serum resistance
2019 (English)In: Journal of Oral Microbiology, ISSN 2000-2297, E-ISSN 2000-2297, Vol. 11, no 1, article id 1536192Article in journal (Refereed) Published
Abstract [en]

Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus belong to the HACEK group of fastidious Gram-negative organisms, a recognized cause of infective endocarditis. A. actinomycetemcomitans is also implicated in aggressive forms of periodontitis. We demonstrated that A. aphrophilus strains, as A. actinomycetemcomitans are ubiquitously serum resistant. Both species encode two Outer membrane protein A paralogues, here denoted OmpA1 and OmpA2. As their respective pangenomes contain several OmpA1 and OmpA2 alleles, they represent potential genotypic markers. A naturally competent strain of A. actinomycetemcomitans and A. aphrophilus, respectively were used to elucidate if OmpA1 and OmpA2 contribute to serum resistance. Whereas OmpA1 was critical for survival of A. actinomycetemcomitans D7SS in 50% normal human serum (NHS), serum resistant ompA1 mutants were fortuitously obtained, expressing enhanced levels of OmpA2. Similarly, OmpA1 rather than OmpA2 was a major contributor to serum resistance of A. aphrophilus HK83. Far-Western blot revealed that OmpA1AA, OmpA2AA, and OmpA1AP can bind to C4-binding protein, an inhibitor of classical and mannose-binding lectin (MBL) complement activation. Indeed, ompA1 mutants were susceptible to these pathways, but also to alternative complement activation. This may at least partly reflect a compromised outer membrane integrity but is also consistent with alternative mechanisms involved in OmpA-mediated serum resistance.

Place, publisher, year, edition, pages
Taylor & Francis, 2019
Keywords
Aggregatibacter actinomycetemcomitans, Aggregatibacter aphrophilus, serum resistance, outer membrane protein A
National Category
Microbiology in the medical area
Identifiers
urn:nbn:se:umu:diva-153103 (URN)10.1080/20002297.2018.1536192 (DOI)000448422100001 ()2-s2.0-85055581204 (Scopus ID)
Available from: 2018-11-07 Created: 2018-11-07 Last updated: 2018-11-07Bibliographically approved
Oscarsson, J., Claesson, R., Lindholm, M., Höglund-Åberg, C. & Johansson, A. (2019). Tools of Aggregatibacter actinomycetemcomitans to Evade the Host Response. Journal of Clinical Medicine, 8(7), Article ID 1079.
Open this publication in new window or tab >>Tools of Aggregatibacter actinomycetemcomitans to Evade the Host Response
Show others...
2019 (English)In: Journal of Clinical Medicine, ISSN 2077-0383, Vol. 8, no 7, article id 1079Article in journal (Refereed) Published
Abstract [en]

Periodontitis is an infection-induced inflammatory disease that affects the tooth supporting tissues, i.e., bone and connective tissues. The initiation and progression of this disease depend on dysbiotic ecological changes in the oral microbiome, thereby affecting the severity of disease through multiple immune-inflammatory responses. Aggregatibacter actinomycetemcomitans is a facultative anaerobic Gram-negative bacterium associated with such cellular and molecular mechanisms associated with the pathogenesis of periodontitis. In the present review, we outline virulence mechanisms that help the bacterium to escape the host response. These properties include invasiveness, secretion of exotoxins, serum resistance, and release of outer membrane vesicles. Virulence properties of A. actinomycetemcomitans that can contribute to treatment resistance in the infected individuals and upon translocation to the circulation, also induce pathogenic mechanisms associated with several systemic diseases.

Place, publisher, year, edition, pages
Basel: MDPI, 2019
Keywords
Aggregatibacter actinomycetemcomitans, cytolethal distending toxin, invasiveness, leukotoxin, outer membrane vesicles, serum resistance
National Category
Medical Biotechnology
Research subject
Infectious Diseases
Identifiers
urn:nbn:se:umu:diva-161754 (URN)10.3390/jcm8071079 (DOI)31336649 (PubMedID)
Available from: 2019-07-25 Created: 2019-07-25 Last updated: 2019-07-29Bibliographically approved
Belibasakis, G. N., Maula, T., Bao, K., Lindholm, M., Bostanci, N., Oscarsson, J., . . . Johansson, A. (2019). Virulence and Pathogenicity Properties of Aggregatibacter actinomycetemcomitans. Pathogens, 8(4), Article ID E222.
Open this publication in new window or tab >>Virulence and Pathogenicity Properties of Aggregatibacter actinomycetemcomitans
Show others...
2019 (English)In: Pathogens, ISSN 2076-0817, Vol. 8, no 4, article id E222Article in journal (Refereed) Published
Abstract [en]

Aggregatibacter actinomycetemcomitans is a periodontal pathogen colonizing the oral cavity of a large proportion of the human population. It is equipped with several potent virulence factors that can cause cell death and induce or evade inflammation. Because of the large genetic diversity within the species, both harmless and highly virulent genotypes of the bacterium have emerged. The oral condition and age, as well as the geographic origin of the individual, influence the risk to be colonized by a virulent genotype of the bacterium. In the present review, the virulence and pathogenicity properties of A. actinomycetemcomitans will be addressed.

Place, publisher, year, edition, pages
Basel: MDPI, 2019
Keywords
Aggregatibacter actinomycetemcomitans, biofilm, cytokine binding factors, cytolethal distending toxin, horizontal gene transfer, leukotoxin, lipopolysaccharides, outer membrane vesicles, proteomic
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:umu:diva-165083 (URN)10.3390/pathogens8040222 (DOI)31698835 (PubMedID)2-s2.0-85074732943 (Scopus ID)
Funder
Swedish Research Council, 2017-01198Västerbotten County Council, 7003193
Available from: 2019-11-09 Created: 2019-11-09 Last updated: 2019-11-26Bibliographically approved
Kemoli, A., Gjørup, H., Nørregaard, M.-L. M., Lindholm, M., Mulli, T., Johansson, A. & Haubek, D. (2018). Prevalence and impact of infant oral mutilation on dental occlusion and oral health-related quality of life among Kenyan adolescents from Maasai Mara. BMC Oral Health, 18(1), Article ID 173.
Open this publication in new window or tab >>Prevalence and impact of infant oral mutilation on dental occlusion and oral health-related quality of life among Kenyan adolescents from Maasai Mara
Show others...
2018 (English)In: BMC Oral Health, ISSN 1472-6831, E-ISSN 1472-6831, Vol. 18, no 1, article id 173Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Infant Oral Mutilation (IOM) includes germectomy and early extraction of primary and permanent incisors and canines, primarily in the lower jaw. The aim of the present study was to examine the prevalence and impact of IOM, involving the removal of mandibular permanent incisors and/or canines, on dental occlusion and Oral Health-Related Quality of Life (OHRQoL) among Kenyan adolescents from Maasai Mara.

METHODS: In a cross-sectional study, 284 adolescents (14-18 yrs. of age) participated in an oral examination and an interview, using a structured questionnaire on age, gender, medical history, and IOM practice. For the analysis of the dental occlusion, participants with IOM, in terms of absence of two or more permanent teeth in the mandibular incisor and/or canine tooth segments (IOM group), were compared to participants who had all six incisors and canines present in the oral cavity (control group). OHRQoL was assessed using child perception questionnaire (CPQ11-14).

RESULTS: The majority of the participants (61%) had been exposed to IOM, among whom 164 (95%) had absence of two mandibular central incisors. More individuals in the IOM group had maxillary overjet exceeding 5 mm than in the control group (50.9% vs. 20%, p <  0.001). Nineteen (11%) subjects in the IOM group had mesial occlusion in contrast to none in the control group (p <  0.001). The mean and median total CPQ scores and the mean and median CPQ domain scores were low in both groups with no significant differences between the groups.

CONCLUSIONS: Approximately two-thirds of the study population presented with IOM, with the majority of them missing two mandibular permanent central incisors. Although some participants with IOM had substantial maxillary overjet and mesial occlusion, only few of them showed substantial effect on their OHRQoL.

Keywords
Avulsion, Ebinyo, Germectomy, Life quality, Malocclusion, Tooth bud
National Category
Dentistry
Research subject
Epidemiology
Identifiers
urn:nbn:se:umu:diva-152845 (URN)10.1186/s12903-018-0631-2 (DOI)000448252100004 ()30355318 (PubMedID)2-s2.0-85055507757 (Scopus ID)
Projects
Maasai Mara Science and Development Initiative
Available from: 2018-10-27 Created: 2018-10-27 Last updated: 2018-12-13Bibliographically approved
Organisations

Search in DiVA

Show all publications