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Gilthorpe, Jonathan
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Publications (2 of 2) Show all publications
Andreae, L. C., Lumsden, A. & Gilthorpe, J. D. (2009). Chick Lrrn2, a novel downstream effector of Hoxb1 and Shh, functions in the selective targeting of rhombomere 4 motor neurons. Neural development, 4, 27
Open this publication in new window or tab >>Chick Lrrn2, a novel downstream effector of Hoxb1 and Shh, functions in the selective targeting of rhombomere 4 motor neurons
2009 (English)In: Neural development, ISSN 1749-8104, Vol. 4, p. 27-Article in journal (Refereed) Published
Abstract [en]

Background; Capricious is a Drosophila adhesion molecule that regulates specific targeting of a subset of motor neurons to their muscle target. We set out to identify whether one of its vertebrate homologues, Lrrn2, might play an analogous role in the chick.

Results; We have shown that Lrrn2 is expressed from early development in the prospective rhombomere 4 (r4) of the chick hindbrain. Subsequently, its expression in the hindbrain becomes restricted to a specific group of motor neurons, the branchiomotor neurons of r4, and their pre-muscle target, the second branchial arch (BA2), along with other sites outside the hindbrain. Misexpression of the signalling molecule Sonic hedgehog (Shh) via in ovo electroporation results in upregulation of Lrrn2 exclusively in r4, while the combined expression of Hoxb1 and Shh is sufficient to induce ectopic Lrrn2 in r1/2. Misexpression of Lrrn2 in r2/3 results in axonal rerouting from the r2 exit point to the r4 exit point and BA2, suggesting a direct role in motor axon guidance.

Conclusion; Lrrn2 acts downstream of Hoxb1 and plays a role in the selective targeting of r4 motor neurons to BA2.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:umu:diva-43081 (URN)10.1186/1749-8104-4-27 (DOI)19602272 (PubMedID)
Note
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Available from: 2011-04-18 Created: 2011-04-18 Last updated: 2012-02-14Bibliographically approved
Scholpp, S., Delogu, A., Gilthorpe, J., Peukert, D., Schindler, S. & Lumsden, A. (2009). Her6 regulates the neurogenetic gradient and neuronal identity in the thalamus. Proceedings of the National Academy of Sciences of the United States of America, 106(47), 19895-19900
Open this publication in new window or tab >>Her6 regulates the neurogenetic gradient and neuronal identity in the thalamus
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2009 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 106, no 47, p. 19895-19900Article in journal (Refereed) Published
Abstract [en]

During vertebrate brain development, the onset of neuronal differentiation is under strict temporal control. In the mammalian thalamus and other brain regions, neurogenesis is regulated also in a spatially progressive manner referred to as a neurogenetic gradient, the underlying mechanism of which is unknown. Here we describe the existence of a neurogenetic gradient in the zebrafish thalamus and show that the progression of neurogenesis is controlled by dynamic expression of the bHLH repressor her6. Members of the Hes/Her family are known to regulate proneural genes, such as Neurogenin and Ascl. Here we find that Her6 determines not only the onset of neurogenesis but also the identity of thalamic neurons, marked by proneural and neurotransmitter gene expression: loss of Her6 leads to premature Neurogenin1-mediated genesis of glutamatergic (excitatory) neurons, whereas maintenance of Her6 leads to Ascl1-mediated production of GABAergic (inhibitory) neurons. Thus, the presence or absence of a single upstream regulator of proneural gene expression, Her6, leads to the establishment of discrete neuronal domains in the thalamus.

Place, publisher, year, edition, pages
The National Academy of Sciences, 2009
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:umu:diva-43080 (URN)10.1073/pnas.0910894106 (DOI)19903880 (PubMedID)
Available from: 2011-04-18 Created: 2011-04-18 Last updated: 2017-12-11Bibliographically approved
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