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Zhang, Y.-P., Wei, H.-H., Wang, W., Xia, R.-Y., Zhou, X.-L., Porr, C. & Lammi, M. (2016). Cross-cultural adaptation and validation of the osteoporosis assessment questionnaire short version (OPAQ-SV) for Chinese osteoporotic fracture females. Clinical Rheumatology, 35(4), 1003-1010.
Open this publication in new window or tab >>Cross-cultural adaptation and validation of the osteoporosis assessment questionnaire short version (OPAQ-SV) for Chinese osteoporotic fracture females
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2016 (English)In: Clinical Rheumatology, ISSN 0770-3198, E-ISSN 1434-9949, Vol. 35, no 4, 1003-1010 p.Article in journal (Refereed) Published
Abstract [en]

The Osteoporosis Assessment Questionnaire Short Version (OPAQ-SV) was cross-culturally adapted to measure health-related quality of life in Chinese osteoporotic fracture females and then validated in China for its psychometric properties. Cross-cultural adaptation, including translation of the original OPAQ-SV into Mandarin Chinese language, was performed according to published guidelines. Validation of the newly cross-culturally adapted OPAQ-SV was conducted by sampling 234 Chinese osteoporotic fracture females and also a control group of 235 Chinese osteoporotic females without fractures, producing robust content, construct, and discriminant validation results. Major categories of reliability were also met: the Cronbach alpha coefficient was 0.975, indicating good internal consistency; the test-retest reliability was 0.80; and principal component analysis resulted in a 6-factor structure explaining 75.847 % of the total variance. Further, the Comparative Fit Index result was 0.922 following the modified model confirmatory factor analysis, and the chi-squared test was 1.98. The root mean squared error of approximation was 0.078. Moreover, significant differences were revealed between females with fractures and those without fractures across all domains (p < 0.001). Overall, the newly cross-culturally adapted OPAQ-SV appears to possess adequate validity and reliability and may be utilized in clinical trials to assess the health-related quality of life in Chinese osteoporotic fracture females.

Place, publisher, year, edition, pages
Springer, 2016
Keyword
Fracture, osteoporosis, quality of life, questionnaire, validation
National Category
Public Health, Global Health, Social Medicine and Epidemiology
Research subject
Public health
Identifiers
urn:nbn:se:umu:diva-118830 (URN)10.1007/s10067-015-3010-2 (DOI)000373155800021 ()26175100 (PubMedID)
Available from: 2016-04-05 Created: 2016-04-05 Last updated: 2017-11-30Bibliographically approved
Yang, L., Zhao, G.-H., Liu, H., Wang, X., Guo, X. & Lammi, M. J. (2016). Field synopsis and meta-analyses of genetic epidemiological evidence for Kashin-Beck disease, an endemic osteoarthropathy in China. Molecular Genetics and Genomics, 291(5), 1823-1833, Article ID 27256326.
Open this publication in new window or tab >>Field synopsis and meta-analyses of genetic epidemiological evidence for Kashin-Beck disease, an endemic osteoarthropathy in China
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2016 (English)In: Molecular Genetics and Genomics, ISSN 1617-4615, E-ISSN 1617-4623, Vol. 291, no 5, 1823-1833 p., 27256326Article in journal (Refereed) Published
Abstract [en]

Kashin-Beck disease (KBD) is a chronic degenerative osteoarthropathy with unclear etiology. To provide current evidence supporting a genetic predisposition for KBD, we conducted a systematic review and meta-analysis of published literature on the genetic epidemiology of KBD. The PubMed, China National Knowledge Infrastructure and Wan Fang Data were searched up to August 2015 for articles published in English and Chinese. Genome-wide and exome sequencing, linkage, and case-control association studies for any genetic variants associated with KBD were included. Meta-analysis was performed for all single nucleotide polymorphisms (SNPs) that were evaluated in two or more studies. The effect size was summarized as odds ratios (ORs) with 95 % confidence intervals (CIs) by fixed and random effects models. A total of 24 articles were systematically reviewed. Eleven short tandem repeats on chromosomes 2, 11 and 12, 34 SNPs in 12 genes, as well as copy number variant 452 were identified as KBD susceptibility factors in individual studies. The meta-analysis of the GPX1 rs1050450, DIO2 rs225014, TrxR2 rs5748469 and HLA-DRB1 rs7745040 failed to reveal any associations with KBD. However, the meta-analysis of HLA-DRB1 rs9275295 allele A was associated with KBD (OR = 1.737, 95 % CI: 1.002-3.012). In addition, seven haplotypes in GPX1, GPX4, HLA-DRB1 and GDF5 genes also showed significant associations with KBD. In conclusions, our study could identify a number of genetic markers associated with KBD. However, the evidence does not currently support a strong association between the specific variants and KBD because of the limited number of studies, and in the future, more rigorous studies are needed to confirm KBD's links with these variants.

Place, publisher, year, edition, pages
Springer Berlin/Heidelberg, 2016
Keyword
Kashin-Beck disease, Genetics, Polymorphism, Systematic review
National Category
Rheumatology and Autoimmunity Medical Genetics
Research subject
Genetics; Medicine, rheumatology
Identifiers
urn:nbn:se:umu:diva-124785 (URN)10.1007/s00438-016-1222-z (DOI)000382145100002 ()27256326 (PubMedID)
Available from: 2016-08-24 Created: 2016-08-24 Last updated: 2018-01-10Bibliographically approved
Yang, L., Liu, H., Guo, X. & Lammi, M. J. (2016). The potential biochemical markers of Kashin-Beck disease: a meta-analysis. Biomarkers, 21(7), 633-638.
Open this publication in new window or tab >>The potential biochemical markers of Kashin-Beck disease: a meta-analysis
2016 (English)In: Biomarkers, ISSN 1354-750X, E-ISSN 1366-5804, Vol. 21, no 7, 633-638 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: The objective of this study is to explore the cytokines in serum, synovial fluid as potential biomarkers of Kashin-Beck disease (KBD) and to further understand the role of these cytokines in the pathogenesis of KBD.

METHODS: A systematic electronic database search was performed from inception up to 15 March 2015. Meta-analysis was performed for cytokines more than one repetition in studies with available data. The effect size was summarized as standardized mean difference (SMD) with 95% confidence intervals (CIs) by a random effect model.

RESULTS: A total of 18 articles were included. The pooled standardized mean differences showed the serum levels of tumor necrosis factor alpha (2.72, 95% CI: 1.8 5-3.59), interleukin-1 beta (1.21, 95% CI: 0.6 1-1.80), and nitric oxide (2.60, 95% CI: 1.5 2-3.68) were significantly higher in adult KBD patients compared with that in healthy controls.

CONCLUSIONS: There was explicit evidence showing that the tumor necrosis factor alpha, interleukin-1 beta and nitric oxide were closely related to the presence of KBD, and these cytokines played a vital role in the pathogenesis of KBD.

Place, publisher, year, edition, pages
Taylor & Francis, 2016
Keyword
Biomarkers, cytokines, Kashin-Beck disease, meta-analysis
National Category
Rheumatology and Autoimmunity Public Health, Global Health, Social Medicine and Epidemiology
Research subject
Public health
Identifiers
urn:nbn:se:umu:diva-119781 (URN)10.3109/1354750X.2016.1171905 (DOI)000385399600009 ()27097773 (PubMedID)
Available from: 2016-04-27 Created: 2016-04-27 Last updated: 2017-11-30Bibliographically approved
Karjalainen, H., Qu, C., Leskelä, S., Rilla, K. & Lammi, M. (2015). Chondrocytic cells express the taurine transporter on their plasma membrane and regulate its expression under anisotonic conditions. Amino Acids, 47(3), 561-570.
Open this publication in new window or tab >>Chondrocytic cells express the taurine transporter on their plasma membrane and regulate its expression under anisotonic conditions
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2015 (English)In: Amino Acids, ISSN 0939-4451, E-ISSN 1438-2199, Vol. 47, no 3, 561-570 p.Article in journal (Refereed) Published
Abstract [en]

Taurine is a small organic osmolyte which participates in cell volume regulation. Chondrocytes have been shown to accumulate and release taurine; in bone, taurine participates in bone metabolism. However, its role in skeletal cells is poorly understood, especially in chondrocytes. This study investigated the regulation of taurine transporter in chondrocytic cells. We examined the transcriptional regulation of the taurine transporter under anisotonia by reporter gene and real-time RT-PCR assays. The effect of providing supplementary taurine on cell viability was evaluated with the lactate dehydrogenase release assay. The localization of the taurine transporter in human chondrosarcoma cells was studied by overexpressing a taurine transporter-enhanced green fluorescent protein. We observed that the transcription of the taurine transporter gene was up-regulated in hypertonic conditions. Hyperosmolarity-related cell death could be partly abolished by taurine supplementation in the medium. As expected, the fluorescently labeled taurine transporter localized at the plasma membrane. In polarized epithelial MDCK cells, the strongest fluorescence signal was located in the lateral cell membrane area. We also observed that the taurine transporter gene was expressed in several human tissues and malignant cell lines. This is the first study to present information on the transcriptional regulation of taurine transporter gene and the localization of the taurine transporter protein in chondrocytic cells.

Place, publisher, year, edition, pages
Vienna, Austria: Springer, 2015
Keyword
taurine transporter, osmotic pressure, cell stretching, chondrocytic cell, human chondrosarcoma
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
cellforskning; Biochemistry
Identifiers
urn:nbn:se:umu:diva-103830 (URN)10.1007/s00726-014-1888-7 (DOI)000349390700011 ()25501278 (PubMedID)
Available from: 2015-06-01 Created: 2015-06-01 Last updated: 2017-12-04Bibliographically approved
Wang, X., Ning, Y., Zhang, F., Yu, F., Tan, W., Lei, Y., . . . Guo, X. (2015). Gene expression signature in endemic osteoarthritis by microarray analysis. International Journal of Molecular Sciences, 16(5), 11465-11481.
Open this publication in new window or tab >>Gene expression signature in endemic osteoarthritis by microarray analysis
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2015 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 16, no 5, 11465-11481 p.Article in journal (Refereed) Published
Abstract [en]

Kashin-Beck Disease (KBD) is an endemic osteochondropathy with an unknown pathogenesis. Diagnosis of KBD is effective only in advanced cases, which eliminates the possibility of early treatment and leads to an inevitable exacerbation of symptoms. Therefore, we aim to identify an accurate blood-based gene signature for the detection of KBD. Previously published gene expression profile data on cartilage and peripheral blood mononuclear cells (PBMCs) from adults with KBD were compared to select potential target genes. Microarray analysis was conducted to evaluate the expression of the target genes in a cohort of 100 KBD patients and 100 healthy controls. A gene expression signature was identified using a training set, which was subsequently validated using an independent test set with a minimum redundancy maximum relevance (mRMR) algorithm and support vector machine (SVM) algorithm. Fifty unique genes were differentially expressed between KBD patients and healthy controls. A 20-gene signature was identified that distinguished between KBD patients and controls with 90% accuracy, 85% sensitivity, and 95% specificity. This study identified a 20-gene signature that accurately distinguishes between patients with KBD and controls using peripheral blood samples. These results promote the further development of blood-based genetic biomarkers for detection of KBD.

Place, publisher, year, edition, pages
Basel, Switzerland: MDPI, 2015
Keyword
Kashin-Beck disease, biomarker, gene expression signature, microarray, peripheral blood mononuclear cells
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Molecular Medicine; Genetics
Identifiers
urn:nbn:se:umu:diva-103826 (URN)10.3390/ijms160511465 (DOI)000356241400135 ()
Available from: 2015-06-01 Created: 2015-06-01 Last updated: 2017-12-04Bibliographically approved
Lappalainen, R., Korhonen, H., Kaitainen, S., Myllymaa, S., Qu, C. & Lammi, M. (2015). Nanostructured coatings for biomedical applications by ultra short pulsed laser deposition (3ed.). In: Mahmoud Aliofkhadzraei (Ed.), Comprehensive guide for nanocoatings technology: Properties and development (pp. 309-323). Nova Science Publishers, Inc..
Open this publication in new window or tab >>Nanostructured coatings for biomedical applications by ultra short pulsed laser deposition
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2015 (English)In: Comprehensive guide for nanocoatings technology: Properties and development / [ed] Mahmoud Aliofkhadzraei, Nova Science Publishers, Inc., 2015, 3, 309-323 p.Chapter in book (Refereed)
Abstract [en]

Ultra short pulsed laser deposition (USPLD) technique is a novel, well-controlled physical vapour deposition method to deposit a wide variety of nanocoatings on solid substrate materials with good adhesion and various surface nanotopographies. Coating materials include ceramics, like Al2O3, TiO2, carbon nitride and amorphous diamond, metals, such as platinum, titanium and Ti6Al4V, as well as polymers, composite materials and so on. In this chapter, we demonstrate and review the possibilities of USPLD for modified biomaterial surfaces in medical applications, such as cell culture plates, electrodes or implants used in orthopaedics and dentistry. The coatings are used to control, e.g., cell growth and proliferation, bacterial adhesion, bioelectrical properties, corrosion, friction and wear.

Place, publisher, year, edition, pages
Nova Science Publishers, Inc., 2015 Edition: 3
Series
Nanotechnology Science and Technology, ISSN 2377-7575
Keyword
Ultra short pulsed laser deposition, nanostructured coatings
National Category
Nano Technology Biomaterials Science Other Physics Topics
Research subject
Materials Science; Physics
Identifiers
urn:nbn:se:umu:diva-108478 (URN)978-1-63482-647-1 (ISBN)978-1-63482-686-0 (ISBN)
Available from: 2015-09-11 Created: 2015-09-11 Last updated: 2017-02-28
Piltti, J., Varjosalo, M., Qu, C., Häyrinen, J. & Lammi, M. (2015). Rho-kinase inhibitor Y-27632 increases cellular proliferation and migration in human foreskin fibroblast cells. Proteomics, 15(17), 2953-2965.
Open this publication in new window or tab >>Rho-kinase inhibitor Y-27632 increases cellular proliferation and migration in human foreskin fibroblast cells
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2015 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 15, no 17, 2953-2965 p.Article in journal (Refereed) Published
Abstract [en]

The idea of direct differentiation of somatic cells into other differentiated cell types has attracted a great interest recently. Rho-kinase inhibitor Y-27632 (ROCKi) is a potential drug molecule, which has been reported to support the gene expressions typical for the chondrocytes, thus restricting their phenotypic conversion to fibroblastic cells upon the cellular expansion. In this study, we have investigated the short-term biological responses of ROCKi to human primary foreskin fibroblasts. The fibroblast cells were exposed to 1 and 10 μM ROCKi treatments. A proteomics analysis revealed expression changes of 56 proteins, and a further protein pathway analysis suggested their association with the cell morphology, the organization, and the increased cellular movement and the proliferation. These functional responses were confirmed by a Cell-IQ time-lapse imaging analysis. Rho-kinase inhibitor treatment increased the cellular proliferation up to twofold during the first 12 h, and a wound model based migration assay showed 50% faster filling of the mechanically generated wound area. Additionally, significantly less vinculin-associated focal adhesions were present in the ROCKi-treated cells. Despite the marked changes in the cell behavior, ROCKi was not able to induce the expression of the chondrocyte-specific genes, such as procollagen α1 (II) and aggrecan.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2015
Keyword
Fibroblast, Label-free quantitative proteomics, Rho-kinase inhibitor Y-27632, actin, time-lapse imaging
National Category
Cell Biology Biochemistry and Molecular Biology
Research subject
Biochemistry; cellforskning
Identifiers
urn:nbn:se:umu:diva-108480 (URN)10.1002/pmic.201400417 (DOI)000360965900009 ()25951301 (PubMedID)
Available from: 2015-09-11 Created: 2015-09-11 Last updated: 2017-12-04Bibliographically approved
Prittinen, J., Jiang, Y., Ylärinne, J., Pakkanen, T., Lammi, M. & Qu, C. (2014). Chondrocyte behavior on nanostructured micropillar polypropylene and polystyrene surfaces. Materials Science and Engineering. C, Materials for Biological Applications, 43, 424-431.
Open this publication in new window or tab >>Chondrocyte behavior on nanostructured micropillar polypropylene and polystyrene surfaces
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2014 (English)In: Materials Science and Engineering. C, Materials for Biological Applications, ISSN 0928-4931, Vol. 43, 424-431 p.Article in journal (Refereed) Published
Abstract [en]

This study was aimed to investigate whether patterned polypropylene (PP) or polystyrene (PS) could enhance the chondrocytes' extracellular matrix (ECM) production and phenotype maintenance. Bovine primary chondrocytes were cultured on smooth PP and PS, as well as on nanostructured micropillar PP (patterned PP) and PS (patterned PS) for 2 weeks. Subsequently, the samples were collected for fluorescein diacetate-based cell viability tests, for immunocytochemical assays of types I and II collagen, actin and vinculin, for scanning electronic microscopic analysis of cell morphology and distribution, and for gene expression assays of Sox9, aggrecan, procollagen α1(II), procollagen α1(X), and procollagen α2(I) using quantitative RT-PCR assays. After two weeks of culture, the bovine primary chondrocytes had attached on both patterned PP and PS, while practically no adhesion was observed on smooth PP. However, the best adhesion of the cells was on smooth PS. The cells, which attached on patterned PP and PS surfaces synthesized types I and II collagen. The chondrocytes' morphology was extended, and an abundant ECM network formed around the attached chondrocytes on both patterned PP and PS. Upon passaging, no significant differences on the chondrocyte-specific gene expression were observed, although the highest expression level of aggrecan was observed on the patterned PS in passage 1 chondrocytes, and the expression level of procollagen α1(II) appeared to decrease in passaged chondrocytes. However, the expressions of procollagen α2(I) were increased in all passaged cell cultures. In conclusion, the bovine primary chondrocytes could be grown on patterned PS and PP surfaces, and they produced extracellular matrix network around the adhered cells. However, neither the patterned PS nor PP could prevent the dedifferentiation of chondrocytes.

Place, publisher, year, edition, pages
Elsevier, 2014
Keyword
Chondrocyte, polypropylene, polystyrene, micropillar, nanostructure
National Category
Materials Chemistry Cell and Molecular Biology
Research subject
Materials Science; cellforskning
Identifiers
urn:nbn:se:umu:diva-103870 (URN)10.1016/j.msec.2014.07.045 (DOI)000342529000052 ()25175232 (PubMedID)
Available from: 2015-06-02 Created: 2015-06-02 Last updated: 2018-01-11Bibliographically approved
Inkinen, S., Liukkonen, J., Ylärinne, J., Puhakka, P., Lammi, M., Virén, T., . . . Töyräs, J. (2014). Collagen and chondrocyte concentrations control ultrasound scattering in agarose scaffolds. Ultrasound in Medicine and Biology, 40(9), 2162-2171.
Open this publication in new window or tab >>Collagen and chondrocyte concentrations control ultrasound scattering in agarose scaffolds
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2014 (English)In: Ultrasound in Medicine and Biology, ISSN 0301-5629, E-ISSN 1879-291X, Vol. 40, no 9, 2162-2171 p.Article in journal (Refereed) Published
Abstract [en]

Ultrasound imaging has been proposed for diagnostics of osteoarthritis and cartilage injuries in vivo. However, the specific contribution of chondrocytes and collagen to ultrasound scattering in articular cartilage has not been systematically studied. We investigated the role of these tissue structures by measuring ultrasound scattering in agarose scaffolds with varying collagen and chondrocyte concentrations. Ultrasound catheters with center frequencies of 9 MHz (7.1–11.0 MHz, −6 dB) and 40 MHz (30.1–45.3 MHz, −6 dB) were applied using an intravascular ultrasound device. Ultrasound backscattering quantified in a region of interest starting right below sample surface differed significantly (p < 0.05) with the concentrations of collagen and chondrocytes. An ultrasound frequency of 40 MHz, as compared with 9 MHz, was more sensitive to variations in collagen and chondrocyte concentrations. The present findings may improve diagnostic interpretation of arthroscopic ultrasound imaging and provide information necessary for development of models describing ultrasound propagation within cartilage.

Place, publisher, year, edition, pages
Elsevier, 2014
Keyword
ultrasound, imaging, articular cartilage, collagen, chondrocyte, scattering, attenuation
National Category
Physical Sciences Radiology, Nuclear Medicine and Medical Imaging
Research subject
Physics; cellforskning
Identifiers
urn:nbn:se:umu:diva-103866 (URN)10.1016/j.ultrasmedbio.2014.03.016 (DOI)000341461100028 ()24972499 (PubMedID)
Available from: 2015-06-02 Created: 2015-06-02 Last updated: 2017-12-04Bibliographically approved
Puhakka, P., Ylärinne, J., Lammi, M., Saarakkala, S., Tiitu, V., Kröger, H., . . . Töyräs, J. (2014). Dependence of light attenuation and backscattering on collagen concentration and chondrocyte density in agarose scaffolds. Physics in Medicine and Biology, 59(21), 6537-6548.
Open this publication in new window or tab >>Dependence of light attenuation and backscattering on collagen concentration and chondrocyte density in agarose scaffolds
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2014 (English)In: Physics in Medicine and Biology, ISSN 0031-9155, E-ISSN 1361-6560, Vol. 59, no 21, 6537-6548 p.Article in journal (Refereed) Published
Abstract [en]

Optical coherence tomography (OCT) has been applied for high resolution imaging of articular cartilage. However, the contribution of individual structural elements of cartilage on OCT signal has not been thoroughly studied. We hypothesize that both collagen and chondrocytes, essential structural components of cartilage, act as important light scatterers and that variation in their concentrations can be detected by OCT through changes in backscattering and attenuation. To evaluate this hypothesis, we established a controlled model system using agarose scaffolds embedded with variable collagen concentrations and chondrocyte densities. Using OCT, we measured the backscattering coefficient (µb) and total attenuation coefficient (µt) in these scaffolds. Along our hypothesis, light backscattering and attenuation in agarose were dependent on collagen concentration and chondrocyte density. Significant correlations were found between µt and chondrocyte density (ρ = 0.853, p < 0.001) and between µt and collagen concentration (ρ = 0.694, p < 0.001). µb correlated significantly with chondrocyte density (ρ = 0.504, p < 0.001) but not with collagen concentration (ρ = 0.103, p = 0.422) of the scaffold. Thus, quantitation of light backscattering and, especially, attenuation could be valuable when evaluating the integrity of soft tissues, such as articular cartilage with OCT.

Place, publisher, year, edition, pages
Institute of Physics Publishing (IOPP), 2014
Keyword
articular cartilage, chondrocyte, collagen, optical coherence tomography, attenuation, backscattering
National Category
Radiology, Nuclear Medicine and Medical Imaging Physical Sciences
Research subject
Physics
Identifiers
urn:nbn:se:umu:diva-103862 (URN)10.1088/0031-9155/59/21/6537 (DOI)000343092900017 ()25310088 (PubMedID)
Available from: 2015-06-02 Created: 2015-06-02 Last updated: 2017-12-04Bibliographically approved
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ORCID iD: ORCID iD iconorcid.org/0000-0002-6181-9904

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