Umeå University's logo

umu.sePublications
EnglishSvenskaNorsk
Change search
ExportLink to record
Permanent link

Direct link
BETA

Project

Project type/Form of grant
Project grant
Title [sv]
RNA och RNP strukturer och deras betydelse för proteintransport och virusinfektion
Title [en]
RNA and RNP structures: elucidating the molecular function of ribonucleoprotein assemblies in protein targeting and viral infections
Abstract [sv]
Our work focuses on structural and functional studies of RNAs and ribonucleoproteins (RNPs) based on X-ray crystallography. The signal recognition particle (SRP) is a multi-subunit RNP which is essential for protein targeting in all living cells. Step-by-step we elucidated structures of the SRP RNA in complex with the SRP proteins. Recently, we published the first crystal structure of the universally conserved SRP core bound to a signal sequence. Currently, the structural analysis of the SRP core bound to the SRP receptor is in progress. To complement our crystallographic studies we use small-angle X-ray scattering (SAXS) as well as mutagenesis and number of biochemical tools to ultimately gain a detailed understanding of SRP function. Flaviviruses are (+) sense RNA viruses that cause serious disease in humans. The coding region in the flaviviral RNA genome is flanked by long 5? and 3? untranslated regions (UTRs) which contain conserved RNA secondary structures. Some of these structures have been functionally characterized and shown to be essential for viral replication, translation and cytopathicity. We are using the dengue virus as a model system. We are aiming to get the first structural insights into these conserved RNAs and their RNPs. The results from these studies we expect to be of great value for a better understanding of viral life cycle and pathogenesis.
Principal InvestigatorSauer-Eriksson, Elisabeth
Coordinating organisation
Umeå University
Funder
Period
2012-01-01 － 2014-12-31
National Category
Structural BiologyBiochemistry and Molecular BiologyBiophysics
Identifiers
DiVA, id: project:1127Project, id: 2011-06009_VR

Search in DiVA

Structural BiologyBiochemistry and Molecular BiologyBiophysics

Search outside of DiVA

GoogleGoogle Scholar
v. 2.43.0
|
WCAG
|
Umeå University Library
|
Register in DiVA
|
Support
|
Search DiVA Portal