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Nitric oxide-triggered remodeling of chloroplast bioenergetics and thylakoid proteins upon nitrogen starvation in Chlamydomonas  reinhardtii
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2014 (English)In: Plant Cell, Vol. 26, no 1, p. 353-72Article in journal (Refereed) Published
Abstract [en]

Starving microalgae for nitrogen sources is commonly used as a biotechnological tool to boost storage of reduced carbon into starch granules or lipid droplets, but the accompanying changes in bioenergetics have been little studied so far. Here, we report that the selective depletion of Rubisco and cytochrome b6f complex that occurs when Chlamydomonas reinhardtii is starved for nitrogen in the presence of acetate and under normoxic conditions is accompanied by a marked increase in chlororespiratory enzymes, which converts the photosynthetic thylakoid membrane into an intracellular matrix for oxidative catabolism of reductants. Cytochrome b6f subunits and most proteins specifically involved in their biogenesis are selectively degraded, mainly by the FtsH and Clp chloroplast proteases. This regulated degradation pathway does not require light, active photosynthesis, or state transitions but is prevented when respiration is impaired or under phototrophic conditions. We provide genetic and pharmacological evidence that NO production from intracellular nitrite governs this degradation pathway: Addition of a NO scavenger and of two distinct NO producers decrease and increase, respectively, the rate of cytochrome b6f degradation; NO-sensitive fluorescence probes, visualized by confocal microscopy, demonstrate that nitrogen-starved cells produce NO only when the cytochrome b6f degradation pathway is activated.

Place, publisher, year, edition, pages
2014. Vol. 26, no 1, p. 353-72
Keywords [en]
Chlamydomonas reinhardtii/*metabolism/physiology/ultrastructure, Cytochrome b6f Complex/genetics/metabolism, Energy Metabolism, Nitric Oxide/metabolism/*pharmacology, Nitrites/metabolism, Nitrogen/*metabolism, Photosynthesis, Proteolysis, Ribulose-Bisphosphate Carboxylase/genetics/metabolism, Thylakoids/*metabolism
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Biological Sciences
Identifiers
URN: urn:nbn:se:umu:diva-144805ISBN: 1532-298X (Electronic) 1040-4651 (Linking) OAI: oai:DiVA.org:umu-144805DiVA, id: diva2:1183508
Note

Wei, Lili Derrien, Benoit Gautier, Arnaud Houille-Vernes, Laura Boulouis, Alix Saint-Marcoux, Denis Malnoe, Alizee Rappaport, Fabrice de Vitry, Catherine Vallon, Olivier Choquet, Yves Wollman, Francis-Andre eng Research Support, Non-U.S. Gov't 2014/01/30 06:00 Plant Cell. 2014 Jan;26(1):353-72. doi: 10.1105/tpc.113.120121. Epub 2014 Jan 28.

Available from: 2018-02-17 Created: 2018-02-17 Last updated: 2018-06-09

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http://www.ncbi.nlm.nih.gov/pubmed/24474630

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Malnoë, A.

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Citation style
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