Umeå University's logo

umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Phospholipid removal for enhanced chemical exposomics in human plasma
Department of Environmental Science, Science for Life Laboratory, Stockholm University, Stockholm, Sweden.
Department of Environmental Science, Science for Life Laboratory, Stockholm University, Stockholm, Sweden.
Department of Environmental Science, Science for Life Laboratory, Stockholm University, Stockholm, Sweden; National Facility for Exposomics, Science for Life Laboratory, Stockholm University, Solna, Sweden.
Department of Environmental Science, Science for Life Laboratory, Stockholm University, Stockholm, Sweden; National Facility for Exposomics, Science for Life Laboratory, Stockholm University, Solna, Sweden.
Show others and affiliations
2023 (English)In: Environmental Science and Technology, ISSN 0013-936X, E-ISSN 1520-5851, Vol. 57, no 28, p. 10173-10184Article in journal (Refereed) Published
Abstract [en]

The challenge of chemical exposomics in human plasma is the 1000-fold concentration gap between endogenous substances and environmental pollutants. Phospholipids are the major endogenous small molecules in plasma, thus we validated a chemical exposomics protocol with an optimized phospholipid-removal step prior to targeted and non-targeted liquid chromatography high-resolution mass spectrometry. Increased injection volume with negligible matrix effect permitted sensitive multiclass targeted analysis of 77 priority analytes; median MLOQ = 0.05 ng/mL for 200 μL plasma. In non-targeted acquisition, mean total signal intensities of non-phospholipids were enhanced 6-fold in positive (max 28-fold) and 4-fold in negative mode (max 58-fold) compared to a control method without phospholipid removal. Moreover, 109 and 28% more non-phospholipid molecular features were detected by exposomics in positive and negative mode, respectively, allowing new substances to be annotated that were non-detectable without phospholipid removal. In individual adult plasma (100 μL, n = 34), 28 analytes were detected and quantified among 10 chemical classes, and quantitation of per- and polyfluoroalkyl substances (PFAS) was externally validated by independent targeted analysis. Retrospective discovery and semi-quantification of PFAS-precursors was demonstrated, and widespread fenuron exposure is reported in plasma for the first time. The new exposomics method is complementary to metabolomics protocols, relies on open science resources, and can be scaled to support large studies of the exposome.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2023. Vol. 57, no 28, p. 10173-10184
Keywords [en]
chemical exposome, high-resolution mass spectrometry, liquid chromatography, multiclass targeted, non-targeted, phospholipid, plasma
National Category
Environmental Sciences
Identifiers
URN: urn:nbn:se:umu:diva-212322DOI: 10.1021/acs.est.3c00663ISI: 001021461300001PubMedID: 37394749Scopus ID: 2-s2.0-85164670955OAI: oai:DiVA.org:umu-212322DiVA, id: diva2:1783871
Funder
Swedish Research Council, 2018-03409Swedish Research Council, 2017-00650Available from: 2023-07-25 Created: 2023-07-25 Last updated: 2023-07-25Bibliographically approved

Open Access in DiVA

fulltext(4429 kB)52 downloads
File information
File name FULLTEXT01.pdfFile size 4429 kBChecksum SHA-512
272eebcf78c4b3c7745572af7b023627b0ae8ecffacfa2fc161b007c154034fa6e0f687e07f2c7111594118450a79f327de8929b915265e5b92c19d602279c8d
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records

Bergdahl, Ingvar

Search in DiVA

By author/editor
Bergdahl, Ingvar
By organisation
Section of Sustainable Health
In the same journal
Environmental Science and Technology
Environmental Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 52 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 110 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf