umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
In Vitro Osteogenic Differentiation of Human Mesenchymal Stem Cells from Jawbone Compared with Dental Tissue
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB), Anatomi. Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB), Anatomi.
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB), Anatomi. Umeå universitet, Medicinska fakulteten, Institutionen för kirurgisk och perioperativ vetenskap.
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB), Anatomi.
2017 (engelsk)Inngår i: Tissue Engineering and Regenerative Medicine, ISSN 1738-2696, Vol. 14, nr 6, s. 763-774Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Autologous bone transplantation is the current gold standard for reconstruction of jawbone defects. Bone regeneration using mesenchymal stem cells (MSC) is an interesting alternative to improve the current techniques, which necessitate a second site of surgery resulting in donor site morbidity. In this study, we compared the osteogenic ability of jawbone MSC (JB-MSC) with MSC from tissues with neural crest origin, namely, the dental pulp, apical papilla and periodontal ligament. All four types of MSC were isolated from the same patient (n = 3 donors) to exclude inter-individual variations. The MSC growth and differentiation properties were characterized. The osteogenic differentiation potential in each group of cells was assessed quantitatively to determine if there were any differences between the cell types. All cells expressed the MSC-associated surface markers CD73, CD90, CD105, and CD146 and were negative for CD11b, CD19, CD34, CD45 and HLA-DR. All cell types proliferated at similar rates, exhibited similar clonogenic activity and could differentiate into adipocytes and osteoblasts. An alkaline phosphatase assay, OsteoImageTM assay for mineralization and qRT-PCR measuring the genes runx2, ALP and OCN, indicated that there were no significant differences in the osteogenic differentiation ability between the various MSCs. In conclusion, we show that from a small segment of jawbone it is possible to isolate sufficient quantities of MSC and that these cells can easily be expanded and differentiated into osteoblasts. JB-MSC appear to be good candidates for future bone regeneration applications in the craniofacial region.

sted, utgiver, år, opplag, sider
Korean Tissue Engineering and Regenerative Medicine , 2017. Vol. 14, nr 6, s. 763-774
Emneord [en]
Stem cells from apical papilla, Dental pulp stem cells, Periodontal ligament stem cells, Stem cells from jawbone, Osteogenic differentiation
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-143573DOI: 10.1007/s13770-017-0071-0ISI: 000417074300011OAI: oai:DiVA.org:umu-143573DiVA, id: diva2:1170702
Tilgjengelig fra: 2018-01-04 Laget: 2018-01-04 Sist oppdatert: 2018-06-09bibliografisk kontrollert

Open Access i DiVA

fulltext(1937 kB)120 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 1937 kBChecksum SHA-512
4077ddf46491548192eb665bf63da445ff1474411e059e31971b38e00d3f3cfacdccbf21b699090b181249b9f526a7d757391449b51b57448b293dd918c403be
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekst

Personposter BETA

Pettersson, Linda F.Kingham, Paul J.Wiberg, MikaelKelk, Peyman

Søk i DiVA

Av forfatter/redaktør
Pettersson, Linda F.Kingham, Paul J.Wiberg, MikaelKelk, Peyman
Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 120 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 190 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf