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Liquid-phase measurements of photosynthetic oxygen evolution
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen. Department of Chemistry - Ångström Laboratory, Uppsala University, UppsalaSweden.
2018 (engelsk)Inngår i: Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029, Vol. 1770, s. 197-211Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

This chapter compares two different techniques for monitoring photosynthetic O2 production: the widespread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We describe how a simple membrane inlet for MIMS can be made out of a commercial Clark-type cell, and outline the advantages and drawbacks of the two techniques to guide researchers in deciding which method to use. Protocols and examples are given for measuring O2 evolution rates and for determining the number of chlorophyll molecules per active photosystem II reaction center.

sted, utgiver, år, opplag, sider
2018. Vol. 1770, s. 197-211
Emneord [en]
Photosynthetic water oxidation, O2 evolution, Photosystem II, Clark-type electrode, Membrane inlet mass spectrometry
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Identifikatorer
URN: urn:nbn:se:umu:diva-152593DOI: 10.1007/978-1-4939-7786-4_11PubMedID: 29978403OAI: oai:DiVA.org:umu-152593DiVA, id: diva2:1255912
Tilgjengelig fra: 2018-10-15 Laget: 2018-10-15 Sist oppdatert: 2018-11-09bibliografisk kontrollert

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