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Aggregatibacter actinomycetemcomitans LPS binds human interleukin-8
Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
Vise andre og tillknytning
2019 (engelsk)Inngår i: Journal of Oral Microbiology, ISSN 2000-2297, E-ISSN 2000-2297, Vol. 11, nr 1Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Various gram-negative species sequester host cytokines using outer membrane proteins or surface modulation by sulfated polysaccharides. An outer membrane lipoprotein (BilRI) of the periodontal pathogen Aggregatibacter actinomycetemcomitans binds several cytokines, including interleukin (IL)-8. Because IL-8 is positively charged at physiological pH, we aimed to determine whether IL-8 interacts with negatively charged lipopolysaccharide (LPS). Binding was investigated using electrophoretic mobility shift assays and microwell-based time-resolved fluorometric immunoassay. LPS from each tested strain of A. actinomycetemcomitans (N = 13), Pseudomonas aeruginosa (N = 1) and Escherichia coli (N = 1) bound IL-8. The K-d value of the A. actinomycetemcomitans LPS-IL-8 interaction varied between 1.2-17 mu M irrespective of the serotype and the amount of phosphorus in LPS and was significantly lower than that of the BilRI-IL-8 interaction. Moreover, IL-8 interacted with whole A. actinomycetemcomitans cells and outer membrane vesicles. Hence, LPS might be involved in binding of IL-8 to the outer membrane of A. actinomycetemcomitans. This raises an interesting question regarding whether other gram-negative periodontal pathogens use LPS for IL-8 sequestering in vivo.

sted, utgiver, år, opplag, sider
TAYLOR & FRANCIS LTD , 2019. Vol. 11, nr 1
Emneord [en]
Lipopolysaccharides, host-pathogen interactions, chemokines, periodontal pathogen, bacterial rulence, outer membrane vesicles
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-154325DOI: 10.1080/20002297.2018.1549931ISI: 000451666300001OAI: oai:DiVA.org:umu-154325DiVA, id: diva2:1271904
Tilgjengelig fra: 2018-12-18 Laget: 2018-12-18 Sist oppdatert: 2018-12-18bibliografisk kontrollert

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