umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Minor pseudopilin self-assembly primes type II secretion pseudopilus elongation
Unité de Génétique moléculaire, Départements de Microbiologie et Biologie Structurale et Chimie, Institut Pasteur, Paris, France.ORCID-id: 0000-0001-9919-0075
Vise andre og tillknytning
2012 (engelsk)Inngår i: EMBO Journal, ISSN 0261-4189, E-ISSN 1460-2075, Vol. 31, nr 4, s. 1041-1053Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

In Gram-negative bacteria, type II secretion systems (T2SS) assemble inner membrane proteins of the major pseudopilin PulG (GspG) family into periplasmic filaments, which could drive protein secretion in a piston-like manner. Three minor pseudopilins PulI, PulJ and PulK are essential for protein secretion in the Klebsiella oxytoca T2SS, but their molecular function is unknown. Here, we demonstrate that together these proteins prime pseudopilus assembly, without actively controlling its length or secretin channel opening. Using molecular dynamics, bacterial two-hybrid assays, cysteine crosslinking and functional analysis, we show that PulI and PulJ nucleate filament assembly by forming a staggered complex in the plasma membrane. Binding of PulK to this complex results in its partial extraction from the membrane and in a 1-nm shift between their transmembrane segments, equivalent to the major pseudopilin register in the assembled PulG filament. This promotes fully efficient pseudopilus assembly and protein secretion. Therefore, we propose that PulI, PulJ and PulK self-assembly is thermodynamically coupled to the initiation of pseudopilus assembly, possibly setting the assembly machinery in motion.

sted, utgiver, år, opplag, sider
2012. Vol. 31, nr 4, s. 1041-1053
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-156771DOI: 10.1038/emboj.2011.454PubMedID: 22157749OAI: oai:DiVA.org:umu-156771DiVA, id: diva2:1291984
Tilgjengelig fra: 2019-02-26 Laget: 2019-02-26 Sist oppdatert: 2019-03-07bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMed

Personposter BETA

Cisneros, David A.

Søk i DiVA

Av forfatter/redaktør
Cisneros, David A.
I samme tidsskrift
EMBO Journal

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 35 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf