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Urea dilution of serum for reproducible anti-HSV1 IgG avidity index
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Virologi.ORCID-id: 0000-0001-9094-319X
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Virologi. Laboratory Medicine, Clinical Microbiology, Umeå University Hospital, Umeå, Sweden.
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Virologi.
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Virologi. Laboratory Medicine, Clinical Microbiology, Umeå University Hospital, Umeå, Sweden.
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2019 (engelsk)Inngår i: BMC Infectious Diseases, ISSN 1471-2334, E-ISSN 1471-2334, Vol. 19, artikkel-id 164Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content in human sera. Human serum from two distinct cohorts; one a biobank collection (Betula) (n = 28), and one from a clinical diagnostics laboratory at Northern Sweden University Hospital (NUS) (n = 18), were assessed for presence of IgG antibodies against HSV1 by a commercially available ELISA-kit. Addition of urea at the incubation step reduces effective binding, and the ratio between urea treated sample and non-treated sample was used to express an avidity index (AI) for individual samples. AI score ranged between 43.2 and 73.4% among anti-HSV1 positive biobank sera. Clinical samples ranged between 36.3 and 74.9%. Reproducibility expressed as an intraclass correlation coefficient (ICC) was estimated at 0.948 (95% CI: 0.900-0.979) and 0.989 (95% CI 0.969-0.996) in the biobank and clinical samples, respectively. The method allows for AI scoring of anti-HSV1 IgG from individual human sera with a single measurement. The least significant change between two measurements at the p < 0.05 level was estimated at 5.4 and 3.2 points, respectively, for the two assessed cohorts.

sted, utgiver, år, opplag, sider
BioMed Central, 2019. Vol. 19, artikkel-id 164
Emneord [en]
Herpes simplex, IgG, Avidity, ELISA, Primary infection, Reactivated infection
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-157213DOI: 10.1186/s12879-019-3769-xISI: 000459030400003PubMedID: 30764767OAI: oai:DiVA.org:umu-157213DiVA, id: diva2:1298903
Tilgjengelig fra: 2019-03-25 Laget: 2019-03-25 Sist oppdatert: 2019-03-25bibliografisk kontrollert

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Olsson, JanJohansson, JörgenHonkala, EmmaLövheim, HugoElgh, Fredrik

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