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Lipoprotein lipase in mouse kidney: effects of nutritional status and high-fat diet
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Fysiologisk kemi.
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Fysiologisk kemi.
Umeå universitet, Medicinska fakulteten, Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM). Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Fysiologisk kemi.
Vise andre og tillknytning
2019 (engelsk)Inngår i: American Journal of Physiology - Renal Physiology, ISSN 1931-857X, E-ISSN 1522-1466, Vol. 316, nr 3, s. F558-F571Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Activity of lipoprotein lipase (LPL) is high in mouse kidney, but the reason is poorly understood. The aim was to characterize localization, regulation, and function of LPL in kidney of C57BL/6J mice. We found LPL mainly in proximal tubules, localized inside the tubular epithelial cells, under all conditions studied. In fed mice, some LPL, colocalized with the endothelial markers CD31 and GPIHBP1 and could be removed by perfusion with heparin. indicating a vascular location. The role of angiopoietin-like protein 4 (ANGPTL4) for nutritional modulation of LPL activity was studied in wild-type and Angptl4(-/-) mice. In Angptl4(-/-) mice, kidney LPL activity remained high in fasted animals, indicating that ANGPTL4 is involved in suppression of LPL activity on fasting, like in adipose tissue. The amount of ANGPTL4 protein in kidney was low, and the protein appeared smaller in size, compared with ANGPTL4 in heart and adipose tissue. To study the influence of obesity, mice were challenged with high-fat diet for 22 wk, and LPL was studied after an overnight fast compared with fasted mice given food for 3 h. High-fat diet caused blunting of the normal adaptation of LPL activity to feeding/fasting in adipose tissue, but in kidneys this adaptation was lost only in male mice. LPL activity increases to high levels in mouse kidney after feeding, but as no difference in uptake of chylomicron triglycerides in kidneys is found between fasted and fed states, our data confinn that LPL appears to have a minor role for lipid uptake in this organ.

sted, utgiver, år, opplag, sider
American Physiological Society , 2019. Vol. 316, nr 3, s. F558-F571
Emneord [en]
angiopoietin-like protein 4, high-fat diet, lipoprotein lipase, mouse, triglyceride uptake
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-158108DOI: 10.1152/ajprenal.00474.2018ISI: 000462008700015PubMedID: 30698048OAI: oai:DiVA.org:umu-158108DiVA, id: diva2:1304524
Forskningsfinansiär
Swedish Research Council, 2015-02942Swedish Heart Lung Foundation, 2016-245-32MTilgjengelig fra: 2019-04-12 Laget: 2019-04-12 Sist oppdatert: 2019-04-12bibliografisk kontrollert

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Nyrén, RakelMakoveichuk, ElenaMalla, SandhyaNilsson, Stefan K.Ericsson, MadeleneOlivecrona, Gunilla

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