umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Francisella tularensis: FupA mutation contributes to fluoroquinolone resistance by increasing vesicle secretion and biofilm formation
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Klinisk bakteriologi. Umeå universitet, Medicinska fakulteten, Molekylär Infektionsmedicin, Sverige (MIMS).
Vise andre og tillknytning
2019 (engelsk)Inngår i: Emerging Microbes & Infections, ISSN 2222-1751, Vol. 8, nr 1Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Francisella tularensis is the causative agent in tularemia for which the high prevalence of treatment failure and relapse is a major concern. Directed-evolution experiments revealed that acquisition of fluoroquinolone (FQ) resistance was linked to factors in addition to mutations in DNA gyrase. Here, using F. tularensis live vaccine strain (LVS) as a model, we demonstrated that FupA/B (Fer-Utilization Protein) expression is linked to FQ susceptibility, and that the virulent strain F. tularensis subsp. tularensisSCHU S4 deleted for the homologous FupA protein exhibited even higher FQ resistance. In addition to an increased FQ minimal inhibitory concentration, LVSΔfupA/B displayed tolerance toward bactericidal compounds including ciprofloxacin and gentamicin. Interestingly, the FupA/B deletion was found to promote increased secretion of outer membrane vesicles (OMVs). Mass spectrometry-based quantitative proteomic characterization of vesicles from LVS and LVS∆fupA/B identified 801 proteins, including a subset of 23 proteins exhibiting differential abundance between both strains which may therefore contribute to the reduced antibiotic susceptibility of the FupA/B-deleted strain. We also demonstrated that OMVs are key structural elements of LVSΔfupA/Bbiofilms providing protection against FQ. These results provide a new basis for understanding and tackling antibiotic resistance and/or persistence of Francisella and other pathogenic members of the Thiotrichales class.

sted, utgiver, år, opplag, sider
Taylor & Francis, 2019. Vol. 8, nr 1
Emneord [en]
Francisella, OMVs, biofilms, antibiotics, fluoroquinolones
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-161719DOI: 10.1080/22221751.2019.1615848ISI: 000473826100001PubMedID: 31164053OAI: oai:DiVA.org:umu-161719DiVA, id: diva2:1339223
Tilgjengelig fra: 2019-07-26 Laget: 2019-07-26 Sist oppdatert: 2019-07-26bibliografisk kontrollert

Open Access i DiVA

fulltext(4280 kB)98 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 4280 kBChecksum SHA-512
42db45b2e97be05fb6ec5462debcbf743e23156599fbcbfb763fc2293c3c343f540533794b56ea098a66dbca3ed2abb656f0739c7853164cf71eb56b13683e81
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekstPubMed

Personposter BETA

Lindgren, HelenaSjöstedt, Anders

Søk i DiVA

Av forfatter/redaktør
Lindgren, HelenaSjöstedt, AndersCoute, Yohann
Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 98 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 265 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf