umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
A new family of nitrate/nitrite transporters involved in denitrification
Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten). Umeå universitet, Medicinska fakulteten, Molekylär Infektionsmedicin, Sverige (MIMS). Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid- Consejo Superior de Investigaciones Científicas, Madrid, Spain.
2019 (engelsk)Inngår i: International Microbiology, ISSN 1139-6709, E-ISSN 1618-1905, Vol. 22, nr 1, s. 19-28Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Denitrifying bacteria carry out nitrate and nitrite respiration inside and outside the cell, respectively. In Thermus thermophilus, nitrate and nitrite transport processes are carried out by major facilitator superfamily (MFS) transporters. The sequence of the nar operon of nitrate-only respiring strains of T. thermophilus includes two tandemly organized MFS transporter genes (narK and narT) of the NarK1 and NarK2 families. Both can function as nitrate/nitrite antiporters, but NarK has been proposed as more specific for nitrate whereas NarT more specific for nitrite. In some nitrate- and nitrite-respiring strains of the same species, a single MFS transporter (NarO) belonging to a different MFS subfamily appears. To analyze the role of this single MFS in the same genetic context, we transferred the two types of nar operon to the aerobic strain HB27, and further included in both of them the ability to respire nitrite. The new denitrifying strains HB27dn, with two MFS, and HB27dp, with a single one, were used to isolate mutants devoid of transporters. Through in trans complementation experiments, we demonstrate that the NarO single MFS works efficiently in the transport of both nitrate and nitrite.

sted, utgiver, år, opplag, sider
Springer-Verlag New York, 2019. Vol. 22, nr 1, s. 19-28
Emneord [en]
Thermus, Denitrification, Nitrate, Nitrite, Transport, MFS
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-162498DOI: 10.1007/s10123-018-0023-0ISI: 000464866200001PubMedID: 30810929OAI: oai:DiVA.org:umu-162498DiVA, id: diva2:1344809
Tilgjengelig fra: 2019-08-22 Laget: 2019-08-22 Sist oppdatert: 2019-08-22bibliografisk kontrollert

Open Access i DiVA

fulltext(2275 kB)22 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 2275 kBChecksum SHA-512
96175c341ef4c00dc4f90574b1627d733e17c50218c04849bf4ac3ab3505d31a6283f2e692662507b718b2d736b8b4c1720a486a4bee2e99dc27625b6050c3e6
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekstPubMed

Personposter BETA

Alvarez, Laura

Søk i DiVA

Av forfatter/redaktør
Alvarez, LauraBerenguer, Jose
Av organisasjonen
I samme tidsskrift
International Microbiology

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 22 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 141 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf