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Conserved nucleotides in an RNA essential for hepatitis B virus replication show distinct mobility patterns
Umeå universitet, Medicinsk fakultet, Medicinsk kemi och biofysik.
Umeå universitet, Medicinsk fakultet, Medicinsk kemi och biofysik.
Vise andre og tillknytning
2007 (engelsk)Inngår i: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 35, nr 20, s. 6854-6861Artikkel i tidsskrift (Fagfellevurdert) Published
sted, utgiver, år, opplag, sider
2007. Vol. 35, nr 20, s. 6854-6861
Identifikatorer
URN: urn:nbn:se:umu:diva-6539DOI: 10.1093/nar/gkm774PubMedID: 17933777OAI: oai:DiVA.org:umu-6539DiVA, id: diva2:146208
Tilgjengelig fra: 2007-12-13 Laget: 2007-12-13 Sist oppdatert: 2018-06-09bibliografisk kontrollert
Inngår i avhandling
1. NMR studies of host-pathogen interactions
Åpne denne publikasjonen i ny fane eller vindu >>NMR studies of host-pathogen interactions
2009 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

This thesis describes the use of Nuclear Magnetic Resonance (NMR) for characterizing two host-pathogen interactions: The behavior of a regulatory RNA of the Hepatitis B virus (HBV) and the attachment of Helicobacter pylori (H. pylori) to the gastric mucosa. NMR is a powerful tool in biomedicine, because molecules ranging from small ligands to biomacromolecules can be studied with atomic resolution. Different NMR experiments are designed to determine structures, or to monitor interactions, folding, stability or motion.

Paper I describes the analysis of the motions of a regulatory RNA of HBV. The NMR structure of the RNA had revealed before that several well-conserved nucleotides adopt multiple conformations. Therefore an analysis of possible underlying motions was undertaken using two different NMR techniques, one of which (off-resonance ROESY) was applied to nucleic acids for the first time. The observed motions suggest an explanation why the structurally poorly defined nucleotides are highly conserved.

In paper II we improved the ROESY NMR experiment, which is used to measure internuclear distances for structure determination of medium-sized molecules. Using a small protein and an organometallic complex as examples, we demonstrated that the new EASY ROESY experiment yields clean spectra that can directly be integrated to derive interatomic distances.

H. pylori, the bacterium involved in peptic ulcer disease and gastric cancer, survives in the harsh acidic environment of the stomach. It possesses many membrane proteins which mediate adherence, raising the question, if their activity is related to membrane composition. In paper III & IV we analyzed therefore the phospholipid composition of H. pylori membranes.

In paper III, an advanced method for the analysis of the phospholipid composition of biological membranes was developed. The two-dimensional semi-constant-time 31P,1H-COSY experiment combines information from phosphorus and hydrogen atoms of phospholipids for their unambiguous identification. Furthermore, the high resolution of the two-dimensional experiment allows the quantification of phospholipids where conventional methods fail.

In paper IV we applied the new experiment to analyze the lipid composition of whole H. pylori cells, their inner and outer membranes, and of vesicles shed by the bacterium. The goal of this study was to characterize the vesicles which are suggested to play a role in the inflammation process. We established that the outer membrane and the vesicles have similar phospholipid compositions, suggesting that the vesicles are largely derived from the outer membrane.

The NMR results presented here elucidate details of molecular systems engaged in pathogenicity, as basis for therapeutic strategies against these pathogens.

sted, utgiver, år, opplag, sider
Umeå: , 2009. s. 48
Serie
Umeå University medical dissertations, ISSN 0346-6612 ; 1275
Emneord
NMR, regulatory RNA, HBV, H.pylori, phospholipids, ROESY
HSV kategori
Forskningsprogram
biokemi
Identifikatorer
urn:nbn:se:umu:diva-25710 (URN)978-91-7264-816-6 (ISBN)
Utgiver:
Medicinsk kemi och biofysik, 901 85, Umeå
Disputas
2009-09-26, KB3A9, plan 3, KBC-huset, Umeå Universität, Umeå, 10:00 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2009-09-08 Laget: 2009-08-31 Sist oppdatert: 2018-06-08bibliografisk kontrollert

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