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Mismatch repair-independent increase in spontaneous mutagenesis in yeast lacking non-essential subunits of DNA polymerase ε
Department of Biology, Tufts University, Medford, Massachusetts, United States of America. (Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden)
School of Biology, Georgia Institute of Technology, Atlanta, Georgia, United States of America. (Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden)
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk kemi och biofysik.
Department of Biochemistry, Tulane University Health Sciences Center, New Orleans, Louisiana, United States of America.
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2010 (engelsk)Inngår i: PLoS genetics, ISSN 1553-7404, Vol. 6, nr 11, s. e1001209-Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Yeast DNA polymerase ε (Pol ε) is a highly accurate and processive enzyme that participates in nuclear DNA replication of the leading strand template. In addition to a large subunit (Pol2) harboring the polymerase and proofreading exonuclease active sites, Pol ε also has one essential subunit (Dpb2) and two smaller, non-essential subunits (Dpb3 and Dpb4) whose functions are not fully understood. To probe the functions of Dpb3 and Dpb4, here we investigate the consequences of their absence on the biochemical properties of Pol ε in vitro and on genome stability in vivo. The fidelity of DNA synthesis in vitro by purified Pol2/Dpb2, i.e. lacking Dpb3 and Dpb4, is comparable to the four-subunit Pol ε holoenzyme. Nonetheless, deletion of DPB3 and DPB4 elevates spontaneous frameshift and base substitution rates in vivo, to the same extent as the loss of Pol ε proofreading activity in a pol2-4 strain. In contrast to pol2-4, however, the dpb3Δdpb4Δ does not lead to a synergistic increase of mutation rates with defects in DNA mismatch repair. The increased mutation rate in dpb3Δdpb4Δ strains is partly dependent on REV3, as well as the proofreading capacity of Pol δ. Finally, biochemical studies demonstrate that the absence of Dpb3 and Dpb4 destabilizes the interaction between Pol ε and the template DNA during processive DNA synthesis and during processive 3' to 5'exonucleolytic degradation of DNA. Collectively, these data suggest a model wherein Dpb3 and Dpb4 do not directly influence replication fidelity per se, but rather contribute to normal replication fork progression. In their absence, a defective replisome may more frequently leave gaps on the leading strand that are eventually filled by Pol ζ or Pol δ, in a post-replication process that generates errors not corrected by the DNA mismatch repair system.

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Public Library of Science , 2010. Vol. 6, nr 11, s. e1001209-
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URN: urn:nbn:se:umu:diva-41111DOI: 10.1371/journal.pgen.1001209ISI: 000284587100023PubMedID: 21124948OAI: oai:DiVA.org:umu-41111DiVA, id: diva2:404705
Tilgjengelig fra: 2011-03-18 Laget: 2011-03-18 Sist oppdatert: 2018-06-08bibliografisk kontrollert

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