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Analyzing p-bodies and stress granules in Saccharomyces cerevisae
Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten).
2010 (engelsk)Inngår i: Guide to yeast genetics: functional genomics, proteomics, and other systems analysis / [ed] Jonathan Weissman, Christine Guthrie, Gerald R. Fink, San Diego: Academic Press, 2010, 2, Vol. 470, s. 619-640Kapittel i bok, del av antologi (Fagfellevurdert)
Abstract [en]

Eukaryotic cells contain at least two types of cytoplasmic RNA protein (RNP) granules that contain nontranslating mRNAs. One such RNP granule is a P-body, which contains translationally inactive mRNAs and proteins involved in mRNA degradation and translation repression. A second such RNP granule is a stress granule which also contains mRNAs, some RNA binding proteins and several translation initiation factors, suggesting these granules contain mRNAs stalled in translation initiation. In this chapter, we describe methods to analyze P-bodies and stress granules in Saccharomyces cerevisiae, including procedures to determine if a protein or mRNA can accumulate in either granule, if an environmental perturbation or mutation affects granule size and number, and granule quantification methods.

sted, utgiver, år, opplag, sider
San Diego: Academic Press, 2010, 2. Vol. 470, s. 619-640
Serie
Methods in Enzymology, ISSN 0076-6879 ; 470
Emneord [en]
cytoplasmic processing bodies, messenger-rna decay, translational repression, human-cells, gene-expression, global analysis, body formation, budding yeast, localization, stability
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-43179DOI: 10.1016/S0076-6879(10)70025-2ISI: 000275827900025PubMedID: 20946828ISBN: 978-0-12-375171-3 (tryckt)ISBN: 978-0-12-375172-0 (tryckt)OAI: oai:DiVA.org:umu-43179DiVA, id: diva2:412356
Tilgjengelig fra: 2011-04-22 Laget: 2011-04-22 Sist oppdatert: 2018-06-08bibliografisk kontrollert

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